• Title/Summary/Keyword: 송사리

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Japanese Medaka, Oryzias latipes as a Test Animal for Marine Ecotoxicological Evaluation (해양생태독성평가를 위한 표준시험생물로서의 송사리(Oryzias latipes)에 관한 연구)

  • Park Gyung Soo;Yoon Seong Jin;Lee Seung Min;Kim Ae Hyang;Park Soung Yun;Kang Duk Young
    • Korean Journal of Environmental Biology
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    • v.23 no.3 s.59
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    • pp.293-303
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    • 2005

  • Japanese medaka, Oryzias latipes is widely distributed in the North East Asia including Korea, Japan and east China, and commonly used for freshwater toxicity tests and cytotoxicological studies worldwide. In this study, a series of experiments were conducted to identify the potential of the fish as a standard test species for saltwater toxicity evaluation such as marine receiving waters, ocean-dumped materials and sediment pore waters etc. Hatching, growth and mortality rates of the fish were estimated with the wide ranges of salinity from freshwater to seawater (35 psu). Direct exposure of the fertilized eggs in freshwater to the wide ranges of salinity (from 0 to 35 psu) without pre- acclimation to the saltwater revealed no significant differences in hatching rates by salinities (p =0.24). On the other hand, medaka larvae hatched in freshwater and exposed to saltwater directly showed high mortality at > 25 psu treatment groups (p < 0.0001). However, there was no significant difference in mortality of medaka larvae hatched in 13.8 and 14.2 psu at the wide ranges of salinities ($0\~35$ psu). Growth rates of medaka larvae hatched in the above two salinities showed no differences in body length either from 0 to 35 psu treatment groups (p =0.64 for 13.8 psu group and p=0.32 for 14.2 psu group). The number of gill chloride cell in medaka larvae sharply increased when the larvae were exposed to high salinity. Reference tests with zinc chloride revealed 96h $LC_{50}=8.84(7.19\~10.87)mg\;L^{-1}$ using 7~10 day old medaka larvae. These were comparable or better sensitivity in comparison with the other standard test species such as North American sheepshead minnow Cyprinodon variegatus. Based on the results of these experiments, hatching rates and larvalmortality of medaka must be good toxicity parameters for seawater bioassay and the species seems to be a good standard species for both the freshwater and seawater toxicity test.

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The Identification of Alpha-Tubulin as a Biomarker for Diazinon Exposure in Medaka Fish (송사리 모델계에서 다이아지논 노출에 대한 생물 지표로서 알파 튜블린의 동정)

  • Kim, Woo-Keun;Lee, Sung-Kyu;Chon, Tae-Soo;Koh, Sung-Cheol;Kim, Jong-Sang
    • Environmental Analysis Health and Toxicology
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    • v.22 no.4
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    • pp.305-312
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    • 2007

  • 환경오염을 신속하게 모니터링하기 위한 생물지표의 개발은 증가하고 있는 오염의 심각성에 비추어 매우 중요한 과제로 여겨지고 있다. 본 연구에서는 독성물질처리에 의하여 선택적으로 발현이 조절되는 단백질의 동정을 통하여 독성물질에 대한 단백질 생물지표를 발굴하고자 시도하였다. 즉, 송사리(Oryzias latipes)를 유기인계 살충제인 다이아지논(diazinon)에 0, 0.1, 1, 5 mg/L 농도로 24시간 노출시킨 후, 머리와 몸통부분으로 나누어 단백질 발현패턴을 분석하였다. 본 시스템에서 다이아지논 처리에 의하여 유의적으로 발현이 증가된 단백질로서 alpha-tubulin, ribonuclease pancreatic precursor, protein hfq 등을 동정하였으며, 이 가운데 alpha-tubulin과 $hsp90{\beta}$의 발현이 다이아지논 농도에 의존적으로 증가하는 것을 semi-quantitative RT-PCR방법으로 확인하였다. 이와 같이 다이아지논 처리에 특이적으로 발현이 증가된 송사리 단백질들은 노출평가를 위한 생물지표로서 개발에 응용될 수 있을 것으로 평가된다.

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Changes in Susceptibility of Killifish (Oryzias latipes) to Three Pesticides with Growth (농약에 대한 송사리 생육단계별 감수성의 변화)

  • Shin, Chun-Chul;Lee, Sung-Kyu;Kim, Young-Bae;Kim, Young-Hwa;Roh, Jung-Koo
    • Korean Journal of Environmental Agriculture
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    • v.6 no.1
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    • pp.50-60
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    • 1987

  • Sensitivity, expressed in the $96h-LC_{50}$ determined with continuous flow system, was compared for eight developmental stages of Oryzias latipes with butachlor, diazinon and fenitrothion. Significance of the laboratory determined $96h-LC_{50}$ in real field situation was evaluated for butachlor using outdoor model agrosystem. In the laboratory tests, the most sensitive stage was post-larva stage IV(7 days after hatching) with $96h-LC_{50}$ of 0.14, 1.4, and 1.6ppm for butachlor, diazinon and fenitrothion, respectively, whereas the least sensitive stage was early embryo stage(1 day after fertilization), and then the susceptibility was somewhat stable after 21days after hatching. In the model agrosystem test, the highest concentration of 0.53ppm of butachlor in water was obtained at the 2nd day after application. The stages IV and VIII showed mortality of over 50% at 96 hours after application. It is concluded that the conventional use of butachlor in the field would cause significant adverse effect on O. latipes. It is suggested that sensitivity test at the early life stages with fish is an important part of the ecotoxicological assessment of chemicals.

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