• Journal of Embryo Transfer
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• v.19 no.3
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• pp.275-282
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• 2004

This study was carried out to examine the effects of development media and those change on in vitro development (IVD) of porcine oocytes matured and fertilized in vitro. Putative embryos, which were matured in vitro in modified NCSU-23 (mNCSU-23) supplemented with 10％ porcine follicular fluid (pFF) and were fertilized in mTBM, were developed in vitro as the experimental scheme. The results are as follows. When porcine putative embryos were cultured in vitro in NCSU-23 or CZB/Pig-MEM, the percentage of oocytes cleaved was not different between two systems, but the percentage of blastocysts in NCSU-23 was significantly higher than in CZB/Pig-MEM (P<0.05). And when porcine putative embryos were cultured in vitro in NCSU-23 during 7 days with or without changing media at day 5, which was supplement with or without 10％ fetal bovine serum(FBS), the percentage of oocytes cleaved, blastocysts at day 6, and the cell number of ICM, TE and total of blastocysts at day 7 were not different among three treatments. As a result of this study, it is supposed that NCSU-23 be more favorable, to develop porcine embryos derived from IVM/IVF, than CZB/Pig-MEM, but that demonstration on the effects of changing medium with fresh one stand in need of the more experiments.

• Journal of Bio-Environment Control
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• v.1 no.1
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• pp.52-60
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• 1992

The optimum management of nutrient solution needs the effective combination of fertilizers as well as the accurate control of nutrient solution. This study was attempt to make a supporting system for effective fertilizer combination by using computer and also to develop a EC predicting equation for keeping the EC of solution within the allowable range after application of combined fertilizers. The supporting system consists of three parts : (1) data bases, (2) rules for deciding the kinds and amounts of fertilizers and (3) main control. With input data, the main control automatically constructs the network connecting the related data bases and subsequently executes the operation of searching proper fertilizers through it. For more effective searching, fertilizers are classified into two levels(level 1 and level 2) in consideration of solubility, price, and frequency in use, and searched in that order. The EC prediction equation, a extended form of the Robinson and Stroke's theoretical equation only available for a binary electrolyte, is suggested for predicting the EC of the nutrient solution containing many kinds of inorganic compounds. The comparison of predicted and measured ECs showed good agreements with the high correlation between the predicted EC decrement by ion interaction and the actual one(limiting EC minus measured EC).

• Korean Journal of Plant Tissue Culture
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• v.26 no.2
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• pp.133-136
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• 1999

Different kinds of cytokinins and auxins were tested for both shoot induction and rooting in a rare species, Abeliophyllum distichum Nakai. BA in WPM medium was the most effective in shoot induction, whereas zeatin seemed to be the most suitable for shoot elongation. Kinetin, at the concentration of 2.0~5.0 mg/L showed an effect in shoot induction, but the effect was inferior to BA and zeatin. Rapid shoot elongation could be achieved when the cultures were maintained on the diffuse light condition (below 500 lux) regardless of cytokinin treatments. For in vitro rooting, IBA was investigated as the best type of auxin tested when half strength GD medium was incorporated. The frequency of rooting using the plant growth regulator and medium just mentioned above was revealed as approximately 90%. In addition, the survival rate of rooted plantlets was almost 100% in an artificial soil mixture.

• KSBB Journal
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• v.11 no.2
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• pp.201-210
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• 1996

The production of polyhydroxyalkanoate(PHA) from glucose by batch and fed-batch culture of Pseudomonas sp. HJ was studied. In batch culture using fermentor, 400 rpm of agitalion speed, 2 vvm of aeration rate, 18 hours of inoculum age, and 5% (vlv) of inoculum size were optimal. PHA production was not increased by deficiency of oxygen. In a batch culture, the final call mass was $6.251g/\ell$, and PHA content was 20% of dry cell weight. In a constant feeding fed-batch culture, cell mass increased to $33.24g/\ell$, and PHA content reached 48.9% of dry cell weight. In an intermittent feeding fed-batch culture, cell mass increased to $37.89g/\ell$, and PHA content reached 53.5% of dty cell weight.

• Polymer(Korea)
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• v.34 no.6
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• pp.522-526
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• 2010

Bacterial cellulose is produced by the bacterium Gluconacetobacter xylinus, which forms a nanofibrous pellicle in its culture medium. We studied properties of the bacterial cellulose such as crystallinity, viscosity, morphology, and mechanical properties according to the carbon source. Static cultures of Gluconacetobacter sp. V6 were performed in three kinds of media: standard Hestrin-Schramm medium, and modified medium with either glycerol or molasses as carbon sources. Cell growth and cellulose yield were increased in the glycerol and molasses media. The culture in the glycerol medium improved the physical properties of cellulose such as crystallinity, intrinsic viscosity, and breaking stress. However, the culture in the molasses medium decreased crystallinity, crystallite size, and intrinsic viscosity of cellulose. In summary, the cellulose yield was remarkably improved in the molasses medium, but with inferior structural properties.

• Applied Biological Chemistry
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• v.41 no.3
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• pp.213-218
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• 1998

A bacterium with potent activity of pigment production and protease was isolated and identified as being Bacillus sp. CS-17. Cell growth, protease activity and pigment production of the strain reached to its maximum point after 24 hrs, 48 hrs, 72 hrs, respectively. The best pigment producing ability of Bacillus sp. CS-17 was shown on basal medium for pigment production added 1.0% soybean. The high effcient conditions for pigment production was obtained at culture of pH 8.5, $37^{\circ}C$ and 72 hours. Among the tested 5 gram positive strains and 6 gram negative strains, weak antibacterial activity of pigment concentrated extracts was appeared against growth of B. subtilis, P. aeruginosa, S. typhimurium, E. aerogenes, B. cereus, A. hydrophila.

• Journal of Bio-Environment Control
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• v.15 no.3
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• pp.264-269
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• 2006

This experiment has investigated the effect of growth, yield and fruit quality of fig plant by different concentration of nutrient solution. Nutrient solution for pig plant were three concentrations of the balanced nutrient formula development by Japanese Horticultural Experiment Station. Plant height, number of leaves, stem diameter and number of fruit per plant were the best at 1/2 concentration. However, leaf length and leaf width did not show any difference in other treatment. Fruit length, fruit diameter and soluble solids did not differ from the different concentration of nutrient solutions. However, the fruit weight of fig plant was heavier by hydroponics than by soil culture in 2nd experiment. Early stage growth of fig plant was better at low concentration of nutrient solution and yield was better at high concentration. The result of this experiment will be utilized in the new application for fig plant hydroponics.

• Korean Journal of Food Science and Technology
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• v.30 no.4
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• pp.941-947
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• 1998

We isolated seventy two lactic acid bacteria and two hundred yeast from traditional Andong sikhe. Microorganisms were isolate from 4 kinds of traditional Andong sikhe. The optimum temperature and pH for growth of sikhe yeast, No. SCS 5, was $30^{\circ}C$ and 4.5, respectively. SCS 5 produced $CO_2$ gas and 1.5% of alcohol in malt extract broth. SCS 5 was identified as S. cersvisiae from the observation of shape of vegetative reproduction, morphological and cultural chararteristics, fermentation and assimilation of carbon sources, and physiological characteristics.

• Proceedings of the Korean Society of Developmental Biology Conference
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• 2003.10a
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• pp.142-142
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• 2003

TGF-$\beta$ super family는 난소를 포함하여 여러 기관 및 조직의 성장에 광범위하게 영향을 미치는 것으로 알려져 있으며, TGF-$\beta$ super family는 난자의 매우 초기에 영향을 미치며, 난포의 성장을 촉진하여 초기의 난자의 형태를 이루는데 중요한 역할을 하는 것으로 알려져 있다. 한편 TIMP-1은 난관상피세포로부터 분비되는 난자의 생리활성 촉진인자로 보고되고 있다. 따라서 본 연구는 한우난포란의 체외성숙에 난자의 생리활성 촉진인자를 이용하여 한우 체외성숙 및 체외수정에 미치는 영향을 구명하고자 실시하였다. 한우 난포란은 도축암소의 난소를 채취하여 회수하였으며, 체외성숙은 HP-TCM를 기본 배양액으로 TGF-$\beta$ 0.1, 1, 10 ng/ml를 첨가하여 실시하였고, TIMP-1은 0.05, 0.5, 5ng/ml를 첨가하여 6, 12, 24시간 동안 체외성숙시켰다. 체외성숙된 난자는 체외성숙율을 검사하기 위하여, 0.1% aceto-orcein으로 염색을 실시하여 핵의 변화를 검사하였다. (중략)

• 한국생물공학회:학술대회논문집
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• 2003.04a
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• pp.348-350
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• 2003

Using acclimated sludges with inorganic nitrogen medium for three months and raw sludges, we investigated characteristics of biological nitrogen removal. As results, the acclimated sludges accomplished nitrification efficiently. But raw sludges have hardly done ammonium-nitrification and there was a increasing of ammonium concentration in flask with raw sludges for established experimental period of 72 hrs.