• KOREAN JOURNAL OF CROP SCIENCE
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• v.65 no.4
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• pp.508-517
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• 2020

This study aimed to determine the minimum period of aeration treatment of co-digestate to develop it as liquid fertilizer and the chemical changes that occur in the aerobic liquefying process. The co-digestates were divided into three types depending on their additives: swine slurry anaerobic digestate (SS AD), swine slurry 70% + cow slurry 30% anaerobic digestate (SS + CS AD), and swine slurry 70% + apple pomace 30% anaerobic digestate (SS + AP AD). The pH of all co-digestates increased rapidly after 3 days of aerobic treatment, but had slightly decreased in SS AD after 9 days and in SS + CS AD and SS + AP AD after 15 days. All co-digestates showed a strongly reduced pH between 27 and 36 days of aeration treatment. SS AD had lower pH value, dissolved oxygen (DO), NH₄-N, and NO₃-N content under aerobic conditions than other co-digestates. To assess the fully decomposed liquid fertilizer, a germination test was performed on the undiluted and diluted co-digestate using the liquid fertilizer germination index (LFGI) method. The relative germination ratio, relative root elongation, and germination index of SS AD were higher than those of the others. When the LFGI method was used for the germination test, all co-digestates showed an appropriate germination index of 70 after 60 days of aeration treatment. Thus, we suggest that the minimum period of aeration treatment for co-digestates might be 60 days to develop the fully decomposed liquid fertilizer.

• Journal of Sericultural and Entomological Science
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• v.27 no.1
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• pp.37-41
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• 1985

The haemolymph protein, digestive fluid proteins and digestive fluid amylase activity of wild silkworm, Theophila mandarina those of the were studied by polyacrylamide gel electrophoresis. In addition, they was also compared with silkworm. 1. 6 main protein bands in female and 7 main protein bands in male were detected in the larval haemolymph of T. mandarina where as 8 and 7 main protein bands in female and male of B. mori were observed. Some differences in the haemolymph protein ands of T. mandarina and B. mori were observed. 2. 15 protein bands and 12 protein bands were found in the larval digestive fluid of T. mandarina and B. mori respectively. Some differences in the mobility of digestive fluid proteins of T. mandarina and B. mori were noticed. 3. Larval digestive fluid amylases were anionic and moved near the tracking dye in both T. mandarina and B. mori. Mobility of the digestive fluid amylases relative to bromophenol blue were 0.019 and 0.020 in T. mandarina and B. mori respectively.

• Journal of the Korea Organic Resources Recycling Association
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• v.28 no.3
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• pp.5-14
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• 2020

This study aims to survey the effect of sulphuric acid (H₂SO₄) treatment for pH adjustment with 6.5 and 7.0 regarding ammonia volatilization on chemical content change in the aerobic liquefying process of co-digesate swine manure and apple pomace. The digestates of swine manure was aerated with 0.3 ㎥ air/㎥·min for 60 days. The untreated digestate showed the increased pH and decreased contents of electrical conductivity (EC) and total nitrogen (T-N). The untreated digestate had a high concentration of NH₃ with 172.6 mg/L, but, ammonia (NH₃) concentration of H₂SO₄-treated digestate was significantly lower than that of untreated digestate. The H₂SO₄-untreated digestate for retaining aeration showed a decreased concentration of 47.2% of ammonium nitrogen. While, the H₂SO₄-treated digestate had a high concentration of ammonium nitrogen compared to the untreated digestate. Also, the H₂SO₄ treatment affected to increase the contents of nitrogen and phosphoric acid content. Therefore, the pH adjustment with H₂SO₄ might be a useful method for the decreased ammonia concentration and nitrogen maintenance in the aerobic liquefying process of swine manure digesate.

• Journal of the Korea Organic Resources Recycling Association
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• v.28 no.2
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• pp.5-13
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• 2020

This study was conducted to evaluate the effect of optimal aeration time of livestock manure slurry with fruits pomace on germination index. Six co-digestates of livestock manure slurry with fruits was aerated with 0.1 ㎥ air/㎥·min for 54 days. The maturity of digestates was evaluated using the germination method. The germination index(GI) of co-digestate of SS + CS + MP was more than 70 at the 30th day of aeration. The GI of co-digestate of SS + CS was more than 70 at the 36th day of aeration. The GI of digestate of swine manure slurry alone was 70 at the 54th day of aeration. The co-digestate of SS + MP caused to shorten 24 days of aeration period to reach GI of 70, compared to swine manure digestate. These results suggest that the germination index of seed could be used to establish the optimal aeration time for co-digestate of liquid fertilizer.

• Journal of Animal Science and Technology
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• v.48 no.4
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• pp.541-554
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• 2006

Present review is to introduce an omasal sampling technique in rumen proteolysis and to consider some information on the omasal sampling technique with particular emphasis on methodological aspects. Use of the omasal sampling technique provides a new opportunity for accurate estimation of rumen metabolism with overcoming limitations of previous in vivo, in vitro and/or in situ methods. The potential advantages of the present technique compared with post-ruminal sampling techniques include following points; 1) only rumen cannulated animals are required, 2) less endogenous nitrogen (N) is contaminated in omasal digesta and 3) omasal digesta are devoid of exposure to acid peptide hydrolysis occurring in the abomasum. Estimates of soluble non-ammonia N (SNAN) in omasal digesta indicate that the assumptions underlying the in situ method that rapidly degradable N fraction can be degraded at an infinite rate and only insoluble dietary N escapes the rumen may be not valid. Quatitatively higher peptide concentration rather than free amino acid and soluble protein in escapable SNAN suggests that hydrolysis of peptide to amino acid may be the rate-limiting step in rumen proteolysis.

• Journal of The Korean Society of Grassland and Forage Science
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• v.36 no.4
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• pp.393-401
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• 2016

Anaerobic digestion is a collection of naturally occurring processes that convert organic matter and liquid residue, so-called digestate. The use of digestate biofertilizers is one of the important components of integrated nutrient management, as they are renewable sources of plant nutrients for sustainable agriculture. Seeds of Italian ryegrass (Lolium multiflorum L.) were germinated in different concentration of Chlorella in order to investigate it's the effect of Chlorella on growth parameters, seed germination and early growth. The experiment using plug tray was conducted at the green house placed in the Sangji University. The experiment consisted of nine treatments including different concentrations of Chlorella sp. culture solution and non-treated control. The germination percentage at the treatment with 25% Chlorella sp. culture solution was greater than that of control. The 50% concentration of Chlorella sp. culture solution was found to promote a better seedling growth in terms of shoot length, fresh weight and dry weight compared to the anaerobic digestate. Results showed that the best concentration of Chlorella culture solution was achieved by the 50% concentration of Chlorella culture solution treatment. As a conclusion, the application of Chlorella culture solution was found to be able to promote the germination and shoots growth of Italian ryegrass.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.23 no.2
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• pp.167-177
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• 1990

Rapid production of sardine sauce using sardine meal koji and autolysate from sardine meat was investigated. The sardine meal koji was prepared by mixing sterillized sardine meal with $10\%$ flour and $10\%$ soy sauce koji, and cultivating the mixture for 48hrs at $30^{\circ}C$ and $80\%$ R. H. For sardine autolysate preparation, chopped sardine was mixed with water (10:8= sardine:water, w/w) and autolyzed for 6hrs at $550^{\circ}C$. The optimum temperature and salinity were $40^{\circ}C$ and $15\%$ for rapid fermentation of sardine sauce. Sardine sauce were prepared experimentally under 10 kinds of conditions and fermented for 20 days. The excellent effects of sardine meal koji and autolysates from sardine meat on rapid processing of sardine sauce were showed in enhancing its flavor and advancing of nitrogenous compounds.

• Journal of Sericultural and Entomological Science
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• v.24 no.2
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• pp.73-80
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• 1983

1. The varietal difference in amylase activity of the digestive fluid of the 5th instar larvae reared on the artificial diet was investigated, using the parent commercial silkworm varieties of Japanese strain. The amylase activity was large different among silkworm varieties. The activity was strong in Hansaeng-1, Jam 115 and Jam 117, medium in Hansaeng-3, Jam 113, Jam 119, and Jam 201, weak in Jam 107, Jam 121 and Gyeongchu. The amylase of the digestive fluid of ten parent commercial silkworm varieties is possible-(ae) type, compared with ＋(＋$\^$ae/) type of Daizo. 2. To investigate the effect of a-amylase pre-treatment of the artificial diet, larvae were fed with the diet treated by a-amylase during 4th-5th instar periods. The blood sugar content and cocoon qualities were slightly higher in the experimental larvae than those in the control, while showing the slight less body weight, amylase activity and dietary efficient.

• Journal of the Korea Organic Resources Recycling Association
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• v.19 no.1
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• pp.123-130
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• 2011

Objectives of this study were to compare the environmental effects of digestates produced in bioenergy village on the rice paddy field for recycling. Digestates were applied to the soils and the soil properties and the crop responses were analyzed according to the standard methods of soil evaluation. Plant height and the number of tiller showed similar results in both the conventional and digestate treated field, and the yield of rough rice was higher in the field treated with digestates than that with chemical fertilizer. The amounts of nitrogen absorbed in straw and grain were larger in the digestates-treated field than chemical fertilizer-treated one, and efficiency of nitrogen applied was shown to be the highest in 100% treated digestate of the pig manure. Exchangeable cation and pH increased in the soil treated with digestate after harvesting, but salt was not accumulated. With these results, it was concluded that resource recycling in green town can be facilitated through the securement of arable lands for the application of digestates and the proper use of these fertilizers. Long-term effects of digestate application on the soil environment should be sustainingly studied.

• Proceedings of the Korea Society of Poultry Science Conference
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• 2004.11a
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• pp.111-112
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• 2004

참옻나무 추출액이 산란계의 생산성과 계란품질, 장내미생물 변화 및 영양소 소화율에 미치는 영향을 구명하고자 산란졔 Isa brown 57주령 480수를 공시하여 8주간 케이지에서 사양시험을 실시하였다. 산란율, 산란량 및 사료요구율은 참옻나무 추출액 5000 ppm 급여구가 산란율이 무첨가 대비 유의적으로 향상되었으며, 전반적으로 추출물 급여구의 산란율이 증가하였다. 평균난중과 사료섭취량은 처리간 차이가 없었다. 난각강도, 난각후도는 추출액 급여구가 무첨가 대비 유의적으로 개선되었으며, 난황색 및 Haugh unit은 전 기간에서 추출액 첨가구가 개선되는 경향을 보였다. 맹장내 유산균과 E. coli는 처리간 차이를 보이지 않았으며, Salmonella는 무첨가 대비 추출액 급여구가 약간 낮은 경향을 보였으나 통계적 유의성은 인정되지 않았다. 영양소 소화율은 옻나무 추출액 급여구가 무첨가 대비 전반적으로 향상되는 결과를 보였다.