• Journal of Animal Science and Technology
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• v.47 no.6
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• pp.937-946
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• 2005

This experiment was conducted to investigate the effects of dietary Bacillus subtilis on growth performance, nutrient digestibility, immunological cells change, fecal noxious gas and carcass meat quality characteristics in finishing pigs. The dietary treatments were 1) CON(basal diet), 2) BS0.1(basal diet+ 0.1% Bacillus subtilis) and 3)BS0.2(basal diet+0.2% Bacillus subtilis). Sixty crossbred(Landrace$\time$ Yorkshire$\time$Duroc) pigs(89.5$\pm$0.11kg average initial body weight) were used in a 42 days growth trial. The pigs were assigned to the treatments according to body weight and each treatment had 5 replicates of 4 pigs per pen in a randomized complete block design. Through the entire experimental period, average daily gain(ADG) and average daily feed intake(ADFI) were not significantly different among the treatments. Pigs fed BS0.1 diet significantly increased their gain/feed compared to pigs fed CON and BS0.2 diets(P<0.05). Also, dry matter(DM) and nitrogen(N) digestibilities were greater in the pigs fed BS0.1 diet than those fed CON diet(P<0.05). There were no significant differences in fecal NH3-N concentration among the treatments. In blood assay for immunological cells change investigations, red blood cells(RBC) counts increased in the pigs fed BS0.2 diet compared to pigs fed CON and BS0.1 diets. There were no significant differences in carcass pH, drip loss, marbling and firmness. However, sensual color and a*(redness) value of meat in the pigs fed BS0.2 diet were higher than in pigs fed CON diet(P<0.05). Therefore, this experiment suggested that Bacillus subtilis supplementation could improve nutrient digestibility, RBC counts and carcass meat color of pigs.

• Journal of Animal Science and Technology
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• v.52 no.4
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• pp.297-304
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• 2010

This study was conducted to investigate the effects of dietary quercetin on feed utilization, blood parameters, and meat quality of Korean native goats. Totally sixteen Korean native goats, 15 kg of average BW aged at 7 months, were employed in the experiment with eight replicates per treatment. One group was fed quercetin at 200 mg/kg level and the other group was fed none as control for 15 days. Dietary inclusion of quercetin did not affect feed intake, water intake, and the amount of urine and feces. Digestibilities of crude fat, NDF, and ADF for 5 days were not affected, but digestibility of crude protein was increased by the dietary inclusion of qurecetin (P<0.05). Quercetin increased rumen total VFA, propionate, and butyrate significantly (P<0.05). Acetate/propionate ratio (A/P) in the quercetin treated group was significantly higher than control. 2,2-Azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) ($ABTS^+$) reducing activity of the loin from goat fed quercetin was higher than that of control. Sensory analysis conducted at 24 hr post mortem revealed that color, texture, and overall acceptability of the loin from goat fed quercetin were significantly preferred to that of control. Feeding quercetin did not influence pH, water holding capacity, 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity, TBARS value, and fatty acid composition of the loin significantly. In conclusion, the dietary inclusion of quercetin increased the digestibility of crude protein, rumen total VFA, propionate, butyrate, and A/P ratio. In addition the higher color and texture preference and ABTS+reducing activity of loin indicating some beneficial effect on enhancement of meat qualityin goats.

• Journal of Animal Science and Technology
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• v.45 no.5
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• pp.787-796
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• 2003

Two experiments were conducted to investigate the effect of germanium biotite (GB) on growth performance, nutrient digestibility and fecal gas emission in pigs. In Exp 1., a total of one hundred nursery pigs (initial body weight 13.12${\pm}$0.15kg) were used in a 21 d growth assay. The five treatments were control (CON, basal diet), GB0.5-200 (basal diet + 0.5% GB, 200mesh), GB1.0-200 (basal diet + 1.0% GB, 200mesh), GB0.5-325 (basal diet + 0.5% GB, 325mesh), GB1.0-325 (basal diet + 1.0% GB, 325mesh). Each treatment had four replicates with five pigs per replicate. ADG, ADFI and gain/feed were not significantly different among the treatments. Fecal NH3-N concentration of pigs fed the GB325 diet was lower than that of pigs fed the GB200 diet (P=0.01). The GB treatments reduced fecal volatile fatty acids significantly compared to the CON (propionic acid, P=0.01; butyric acid, P=0.01; acetic acid, P=0.02). Especially, fecal propionic acid concentration of pigs fed the GB325 diets was lower than that of pigs fed the GB200 diets (P=0.02). In Exp 2., a total of seventy five pigs (initial body weight 21.18${\pm}$0.15kg) were used in a 28 d growth assay. The treatments were same as described for Exp. 1. Each treatment had five replicates with three pigs per replicate. The GB1.0 treatments significantly increased the ADG compared to the GB0.5 treatments (P=0.03). The DM and N digestibility of pigs fed the GB1.0 diets were higher than that for pigs fed the GB0.5 diets (P=0.01). Also, the Ca digestibility of pigs fed the GB diets was higher than that for pigs fed the CON diets (P=0.01). The fecal NH3-N concentrations for the GB treatments were lower than that for the CON (P=0.01). The GB325 treatments significantly decreased the fecal NH3-N concentration compared to the GB200 treatments (P=0.03). The fecal butyric acid concentration for the GB325 treatments was lower than that for the GB200 treatment (P=0.04). In conclusion, the results obtained from these feeding trials suggest that the dietary GB for nursery pigs affects fecal noxious gas emission. In growing pigs, dietary GB was effective to improve ADG and decrease fecal noxious gas emission.

• Korean Journal of Weed Science
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• v.3 no.1
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• pp.14-22
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• 1983

To study ecological characteristics of Cyperus serotinus occuring in Korea its propagules were collected from 6 locations from the northern part to the southern part of Korea (Chuncheon, Suweon, Iri, Jeonju, Gwangju, Milyang) in 1981, cultured and replanted 4 times (May 20, June 5, June 20, July 5) in 1982. They flowered from August 10 to August 29 in the plants planted on May 20 and from August 22 to September 4 in the plants planted on July 5. Plant height, number of tillers and top fresh weight were 85-100cm, 375-1,500 tillers/$m^2$ and 500-1,750g/$m^2$, respectively, when they were planted on May 20, and 58-67cm, 300-625 tillers/ $m^2$ and 125-250g/$m^2$, respectively, when they were planted on July S. Weight of seeds and number of rhizomes per plant were 20-50g/$m^2$ and 20.75-61, respectively, whey, they were planted on May 20, and 5-17.5g/$m^2$ and 51.5-80.25 when they were planted on July 5. Local collections showed. the same morphological characteristics at the level of species identification, but there existed variations among the local collections. Cyperus serotinus from Chuncheon and Suweon were longer in the length of inflorescence, than those from Gwangju and Milyang and rhizomes from Chuncheon and Suweon were thicker than the others. Each of local collections may be regarded as different ecotype based on the above mentioned differences in morphology, growth and flowering response to the planting date. The results appear to imply that Cyperus serotinus weeds occuring in various locations of Korea are different one another in competitive ability with rice crop.

• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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• v.7 no.3
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• pp.181-194
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• 2002

Parasitism is a one-sided relationship between two organisms in which one benefits at the expense of the other. Parasitic dinoflagellates, particularly species of Amoebophrya, have long been thought to be a potential biological agent for controlling harmful algal bloom(HAB). Amoebophrya infections have been reported for over 40 species representing more than 24 dinoflagellate genera including a few toxic species. Parasitic dinoflagellates Amoebophrya spp. have a relatively simple life cycle consisting of an infective dispersal stage (dinospore), an intracellular growth stage(trophont), and an extracellular reproductive stage(vermiform). Biology of dinospores such as infectivity, survival, and ability to successfully infect host cells differs among dinoflagellate host-parasite systems. There are growing reports that Amoebophrya spp.(previously, collectively known as Amoebophrya ceratii) exhibit the strong host specificity and would be a species complex composed of several host-specific taxa, based on the marked differences in host-parasite biology, cross infection, and molecular genetic data. Dinoflagellates become reproductively incompetent and are eventually killed by the parasite once infected. During the infection cycle of the parasite, the infected host exhibits ecophysiologically different patterns from those of uninfected host in various ways. Photosynthetic performance in autotrophic dinoflagellates can be significantly altered following infection by parasitic dinoflagellate Amoebophrya, with the magnitude of the effects over the infection cycle of the parasite depending on the site of infection. Parasitism by the parasitic dinoflagellate Amoebophrya could have significant impacts on host behavior such as diel vertical migration. Parasitic dinoflagellates may not only stimulate rapid cycling of dissolved organic materials and/or trace metals but also would repackage the relatively large sized host biomass into a number of smaller dinospores, thereby leading to better retention of host's material and energy within the microbial loop. To better understand the roles of parasites in plankton ecology and harmful algal dynamics, further research on a variety of dinoflagellate host-parasite systems is needed.

• Journal of The Korean Society of Grassland and Forage Science
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• v.39 no.4
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• pp.258-263
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• 2019

Near infrared reflectance spectroscopy (NIRS) has become increasingly used as a rapid and accurate method of evaluating some chemical compositions in forages. The objective of this study was to evaluate the potential of NIRS, applied to imported forage, to estimate the moisture and chemical parameters for imported hays. A population of 392 imported hay representing a wide range in chemical parameters was used in this study. Samples of forage were scanned at 1 nm intervals over the wavelength range 680-2500nm and the optical data was recorded as log 1/Reflectance(log 1/R), which scanned in intact fresh condition. The spectral data were regressed against a range of chemical parameters using partial least squares(PLS) multivariate analysis in conjunction with spectral math treatments to reduced the effect of extraneous noise. The optimum calibrations were selected based on the highest coefficients of determination in cross validation(R²) and the lowest standard error of cross-validation(SECV). The results of this study showed that NIRS predicted the chemical parameters with very high degree of accuracy. The R² and SECV for imported hay calibration were 0.92(SECV 0.61%) for moisture, 0.98(SECV 0.65%) for acid detergent fiber, 0.97(SECV 0.40%) for neutral detergent fiber, 0.99(SECV 0.06%) for crude protein and 0.97(SECV 3.04%) for relative feed value on a dry matter(%), respectively. Results of this experiment showed the possibility of NIRS method to predict the moisture and chemical composition of imported hay in Korea for routine analysis method to evaluate the feed value.

• Korean Journal of Poultry Science
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• v.30 no.3
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• pp.197-202
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• 2003

Feeding probiotics in broiler chicks still critical in several aspects. Thus, this study was conducted to investigate the impact of feeding multiple probiotics on performance, intestinal microflora, blood cholesterol and ND antibody vaccine titer in broiler chicks. Three hundred twenty one day old male broiler chicks(cobb ${\times}$ cobb) were divided into four levels of multiple probiotics(0, 0.1, 0.2, 0.3%) with five replicates for 35 days. Basal diets contained 21.5, 19.0% CP and 3,100 kcal/kg ME for starting and finishing period, respectively. Weight gain, feed intake and feed conversion were measured weekly. The number of Salmonella, E. coli, Lactobacillus, and yeast were examined from ileum and cecum at the end of experiment. ND vaccine titer, cholesterol were detected from sera. Weight gain of birds fed probiotics were 669.33, 679.75 at the level of 0.1 and 0.2% supplemental groups for starting period. It was also improved in those treatments for finishing period and higher than control for total period. Feed conversion tended to be improved compared to that of control by the supplementation of probiotics for the first three weeks and seemed to show the similar tendency for the rest of two weeks. It was 1.611, 1.621 for the entire feeding period and improved compared with control. Total salmonella, was not decreased in ileal digesta of birds fed the probiotics compared with control, whereas the number of yeast increased in 0.1% treatment. However, the number of Lactobacillus and yeast in cecum was higher than control. Even though the blood cholesterol seem to high in 0.1% probiotics treatment, the ratio of HDL to total cholesterol showed higher than control. ND vaccine titer of birds fed probiotics were significantly higher than control (P<0.05). These results 0.1% multiple probiotics would be possible to improve the performance of broiler chicks and ND vaccine titer.

• Korean Journal of Organic Agriculture
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• v.18 no.3
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• pp.401-418
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• 2010

This experiment was selected a $\beta$-glucan producing mushroom strain and developed industrial media, and used to $\beta$-glucan as an alternative for antibiotics in weaned pigs. Yields of mycelial biomass and extracellular $\beta$-glucan from Youngji (Ganoderma lucidum) mushroom was 8.52g/L and 4.49g/L respectively. Also, we prepared optimum formula for mushroom cultivations. A total of 144 pigs ($8.6{\pm}0.9$ kg average body weight, weaned $20{\pm}3$ days of age) were allotted to 4 different treatment groups and replicated 4 times with 8 pigs per replicate in randomized complete block design. Treatments were T1) NC (negative control, basal diet), T2) PC (positive control, basal diet+0.255% antibiotics), T3) NC+0.2% $\beta$-glucan and T4) PC+0.2% $\beta$-glucan. The T2 and T4 treatments were significantly higher in feed efficiency by antibiotics group (p<0.05), however, there was no significant differences in terms of average daily gain (ADG) and average daily feed intake (ADFI) during phase I (0~14 days). In phase II (15~28 days), Pigs fed with antibiotics and $\beta$-glucan (T4) had greater ADG than other treatments (p<0.05), while no differences were observed in ADFI and feed efficiency. During the whole experiment period, the ADG of T4 treatment was higher than other treatments. Pigs fed with $\beta$-glucan (T3 and T4) had greater diarrhea score and moisture content than other treatments (p<0.05). Pigs fed with $\beta$-glucan (T3 and T4) had greater moisture content than other treatments (p<0.05). However, there was no significant differences in diarrhea score and mortality of weaned pigs. There was marginal reductions in feed cost measured feed cost per weight gain used in antibiotics and $\beta$-glucan added diet during phase I. In the second phase, the treatment supplemented with antibiotics had a significantly lower feed cost per weight gain compared to the other treatments. The results from these experiments suggests that $\beta$-glucan is likely able to improve the growth performance, and reduce feed cost although they do not have similar effects like antibiotics in weaning pigs.

• Proceedings of the Korea Society of Poultry Science Conference
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• 2002.11a
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• pp.64-84
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• 2002

These studies were conducted to evaluate the Properties of lactic acid Producing bacteria(LAB), isolated from broiler and laying hens cecum and select the optimum strains to improve the performance, environment of poultry house, immunity, and intestinal microflora of broiler and laying hens. In experiment I , 23 LAB strains were isolated from broiler and laying hens cecum as a colony form. Six strains were selected by acid tolerance, bile salt tolerance, viability, enzyme release, antagonism, and antibiotics susceptibility. In Experiment II, selected LABs from Ex. 1 were conducted to investigate the effects of feeding various Lactobacillus on performance, nutrients digestibility, intestinal microflora, villi development and observation of epithelium surface, blood chemicals and fecal noxious gas of broiler chicks. One thousand eighty one day old broiler chicks were fed into Lactobacillus crispatus avibrol(LCB), Lactobacillus reuteri avibro2(LRB), Lactobacillus crispatus avihen1(LCH), and Lactobacillus vaginalis avihen2(LVH) at the level of 10$^4$ and 10$\^$7/cfu/g diet. Weight gam of chicks fed Lactobacillus tended to increase from the first week and was higher from 50 to 100g in Lactobacillus treatments than control. Feed intake and feed conversion were not statistically different of all treatments. Dry Matter digestibility of Lactobacillus treatments was prone to improve compared to that of control, but was not significantly different. Protein and Ca digestibility were also tended to improve in Lactobacillus treatments relative that of control. Lactobacillus treatments showed improved tendency in crude ash and fat compared to those of control, whereas phosphorus digestibility was not consistency. Nutrients digestibilities of bird fed LCH were superior to those of other treatments, It showed significantly higher in Ca and P digestibility than control(P〈0.05). Total Lactobacillus spp. of birds fed various Lactobacillus was significantly higher in illeum for five weeks(P〈0.05), but was not different at cecum. Yeast was thought to be not completely attached to intestinal lumen for one week. However, total number of yeast was significantly increased in cecum and illeum of three weeks old chicks (P〈0.05). The number of anaerobes exhibited to tendency the increase in Lactobacillus treatments from one week old of age at both ileum and cecum.

• Korean Journal of Poultry Science
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• v.29 no.3
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• pp.205-211
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• 2002

A feeding trial was conducted to study the effects of a live yeast, Pichia farinosa culture(PF), on the production performance and intestinal microflora in laying hens. One hundred and sixty ISA Brown layers, 21 weeks of age, were randomly allotted to four dietary treatments, with four replicates per treatment. Dietary treatments consisted of four levels (0, 0.1, 0.3, and 0.5%) of PF added to a com-soybean meal based diet. Egg production, egg weight, feed intake and fred conversion ratio(FCR) were measured. Egg qualifies were examined at 25th and 29th weeks of age. A metabolism trial was conducted following the feeding trial, during which intestinal microflora, nutrient digestibility and fecal NH3 gas emission were measured. Egg production of birds fed 0.1 and 0.3% PF were significantly higher than those from birds fed 0 and 0.5% PF(P<0.05). Daily egg mass of 0.3% PF increased significantly compared to that of 0% PF. There was no difference in egg weight among all treatments. Feed conversion ratio was significantly improved as the PF level increased. No significant difference was found in eggshell quality and Haugh unit at both 25 and 29 weeks of age. Viable count of ileal Lactobacillus spp. increased significantly as the rf level increased. However, the total number of yeast and anaerobes in ileum were similar among all treatments. Cecal Lactobacillus spp. and yeast counts showed no difference among all treatments. Fecal NH$_{3}$ gas emission of layers fed PF decreased significantly by the PF supplementation. From the result of this experiment, it could be concluded that dietary supplementation of the live yeast Pichia farinosa improves the laying performance and decreases the fecal ammonia gas emission.