• Journal of the Society of Cosmetic Scientists of Korea
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• v.44 no.1
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• pp.95-101
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• 2018

The epidermis which is stratified by epithelial tissue renewal based on keratinocyte differentiation protects the organism from various environmental insults by forming a physical barrier. Autophagy is a mechanism which mediates lysosomal delivery and degradation of protein aggregates, damaged organelles and intracellular microorganisms. Recent reports have shown that autophagy has critical roles for proper terminal differentiation to stratum corneum via removing metabolic organelles and nuclei. However, whether increasing autophagy can activate epidermal differentiation is unknown. Here, we screened a library of natural single compounds and discovered that betaine specifically increased the LC3 positive cytosolic punctate vesicles and LC3-I to LC3-II conversion in HaCaT human keratinocyte cell line, indicating increased autophagy flux. mTOR pathway, which negatively regulates autophagy, was not affected by betaine treatment, suggesting betaine-induced autophagy through an mTOR-independent pathway. Betaine-induced autophagy was also observed in primary human keratinocyte and skin equivalent. Furthermore, epidermal thickness was increased in skin equivalent under betaine treatment. Overall, our finding suggests that betaine as a novel regulator of autophagy may induce epidermal turnover and improve the skin barrier abnormality of the aged epidermis.

• Korean Journal of Plant Taxonomy
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• v.40 no.4
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• pp.226-233
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• 2010

An intensive study of the embryology of Gymnospermium microrrhynchum was conducted to provide information regarding a discussion of the phylogenetic relationships of the genus, which is yet unstudied. Our results indicated that Gymnospermium is similar to other genera of Berberidaceae in terms of its embryological features. Nevertheless, newly reported and unique features are the well-developed endothelium and the undifferentiated seed coat type. Until the study of Gymnospermium, it may have been considered to be closer to Caulophyllum and Leontice in the tribe Leonticeae. These three genera share many morphological features as well as molecular similarities, by which they are kept in the same tribe, Leonticeae. However, very little detailed embryological data regarding these genera have been published thus far. Gymnospermium was characterized according to the basic type of anther wall formation as well as its glandular tapetum, successive cytokinesis in the microspore mother cell, two-celled mature pollen grains, anatropous and crassinucellate ovules with a nucellar cap, well-developed endothelium, its Polygonum type of embryo sac formation, its nuclear type of endosperm formation, and its undifferentiated seed coat type. In comparison with Nandina, there are many differences, such as the dehiscence of the anther, the cytokinesis in the microspore mother cells, the shape of the megaspore dyad, and the seed characteristics. Although we had no available detailed embryological information regarding Caulophyllum and Leontice, which are genera that are more closely related to Gymnospermium, we could deduce from the phylogenetic relationship that Gymnospermium, Caulophyllum, and Leontice are more closely related to each other than other genera of Berberidaceae on the basis of the seed characteristics.

• Proceedings of the Korea Concrete Institute Conference
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• 2008.04a
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• pp.853-856
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• 2008

Lightweight foamed concretes are mainly used in apartment building construction for building room floor insulation, sound proof and height difference adjustment, etc. However, existing lightweight foamed concretes have problems like volume reduction by foam removal and excessive crack occurrence, etc, and for compensation, they developed improved concrete binders for lightweight foamed concrete with special characteristics by adding admixture materials used in concrete manufacturing. Therefore, this study reviewed the possibility of its practical use by analyzing all the engineering characteristics after producing imitation member proposed as actual binders and piling lightweight foamed concrete as improved lightweight foamed concrete binder through prior study, the results are as follows. Plain in which various pulverulent materials are mixed showed about 230mm of flow value, satisfying the target flow value, and at 100mm member, about 4mm of settlement occurred, showing a settlement depth reduction effect double the OPC. On strength, OPC showed highest value, but the three levels all showed strengths above the specified value of KS standard 0.5 grade. From the analysis of drying shrinkage member crack, plain, about 0.1mm, was shown very excellent against drying shrinkage crack.

• Korean Journal of Agricultural Science
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• v.39 no.4
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• pp.497-502
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• 2012

Since the year 2000, the production and consumption of broccoli have rapidly increased in Korea. And, the average production area and amount were about 1,700 ha and 29,000 ton for the past 5 years. Even with the increase of these cultivation and consumption, more than 95% of the broccoli seeds are currently imported from foreign countries such as Japan and Netherlands. Therefore, development of domestic broccoli varieties is needed to relieve Korean farmers' production cost for broccoli. In this situation, National Institute of Horticultural and Herbal Science (NIHHS) of Korea has tried to develop F1 hybrid varieties from elite lines that were obtained by microspore cultivation method from 2008. As the results, about 850 lines of broccoli were obtained and self-pollinated. Then their ploidy levels of the genome were confirmed to select double haploid (D.H.) lines. And the D.H. lines' horticultural traits were evaluated in open field. After the selection of 17 elite D.H. lines, they were cross-pollinated with a male sterile (MS) line to produce F1 hybrid seeds. After 2 to 3 years field trials of these F1 hybrid varieties at the area of Suwon, Gangneung, and Jeju respectively, two hybrid varieties such as 'Wongyo8011' and 'Wongyo8012' are selected for the application of variety protection. With these 4 years of research, we found that the microspore cultivation method is a powerful tool for the conventional breeding program, especially for the development of various inbred lines and even F1 hybrid varieties in short time.

• Korean Journal of Plant Taxonomy
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• v.49 no.4
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• pp.300-318
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• 2019

Many infraspecific taxa within Lespedeza maximowiczii and hybrids with related species have been described, but taxonomic verification remains controversial. We examined the morphological traits of hybrids (L. chiisanensis and L. patentibicolor) and infraspecific taxa (var. tomentella, elongata, and tricolor) and analyzed their genetic structures using microsatellite loci. Flower and leaflet shapes in var. tomentella and elongata were within the range of variation of those in var. maximowiczii, and individuals in the two former varieties were grouped into var. maximowiczii. Lespedeza maximowiczii var. tricolor was similar to L. buergeri in terms of the structure and flower color, whereas the leaflet and bracteole shapes of var. tricolor were similar to those of var. maximowiczii. Based on the genetic structure (K = 3), var. tricolor had a mixed lineage with L. maximowiczii and L. buergeri. In addition, these formed a distinct lineage at K = 5. For two hybrids, the flower and leaflet structure in L. chiisanensis did not differ from those in L. maximowiczii, whereas the flowers of L. patentibicolor were within the range of variation of L. bicolor. In addition, L. chiisanensis and L. patentibicolor were assigned to L. maximowiczii and L. bicolor, respectively, based on the genetic structure. We treated var. tomentella and elongata as a forma, f. friebeana, because L. friebeana preceded var. tomentella, whereas var. tricolor was treated as a distinct species, L. tricolor. Lespedeza chiisanensis was recognized as a synonym of L. maximowiczii. Lespedeza patentibicolor was considered to be L. bicolor.

• Food Science and Preservation
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• v.20 no.1
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• pp.7-13
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• 2013

Mushrooms have a shorter shelf-life than most vegetables because of their very high respiration rates, sensitivity to enzymatic browning and susceptibility to microbial spoilage. This study was conducted to investigate effects of various packaging materials and precooling on the quality of mushrooms (Agaricus bisporus). Mushrooms were precooled at $4^{\circ}C$ for three hours and packaged using the following packaging materials; 1) polyethylene (PE) film bags of 0.03 mm thickness, 2) polypropylene (PP) film bags of 0.03 mm thickness, and 3) polystyrene (PS) tray+polyvinyl chloride (PVC) wrapper. The physiological changes (weight loss, gas composition, color, firmness, and sensory evaluation) associated with postharvest deterioration were monitored for 17 days at $10^{\circ}C$. The results showed that the PP film bag maintained quality of mushrooms most effectively, especially PP film bags inhibited decreasing firmness. The samples also exhibited smaller decreases in weight loss rate (0.57%) and Hunter L value (84.44) than PS tray+PVC wrapper (7.73%, 82.19) and PE film bags (0.89%, 82.96). Sensory evaluation level in all samples remained relatively constant during the first 5 days of storage. However, PE film bags and PS tray+PVC wrapper showed lower score of flavor, texture and color than PP film bags after 8 days of storage. This study suggested that PP film bag packaging effectively extends shelf-life of mushrooms during storage.

• Proceedings of the Ginseng society Conference
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• 1988.08a
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• pp.1-10
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• 1988

Previously. we reported that the intraperitoneal administration of ginseng crude saponin increased: (I) nuclear RNA polymerase activity. (2) nuclear RNA synthesis. (3) cytoplasmic RNA synthesis. (4) cytoplasmic heavy polyrioosome content. (5) amino acid incorporation in vitro of microsome and polysome isolated rat liver. and (6) the incorporation rate of labeled amino acids into serum protein. In addition, a spectacular increase in the rough endoplasmic reticulum of hepatocyte administered crude saponin for four weeks orally was shown through electron microscopy. An increase in polysomal content in membrane-hound ribosome was shown through ultracentrifugation. Recently, successive intraperitoneal. administration .of $ginsenosid-Rb_2$ was given to streptozotocin (STZ) diaoetic rats of hypoproteinemia. The blood urea nitrogen and hepatic urea concentration were decreased significantly. The total protein and alhumin levels in the serum were increased in comparison to control values. In contrast. the $ginsenoside-Rb_2$ treated group of STZ diahetic rats showed a significant increase in liver RNA. total ribosome and membrane-bound ribosomal contents. The administration of $ginsenoside-Rb_2$ increased the incorporation rate of labeled - precursor into total serum protein. Additionally $ginsenoside-Rb_2$ improved the nitrogen balance of diabetic rats. On the bases of these experimental results, ginseng saponin has a metabolic stimulatory or anabolic action on RNA and protein synthesis.

• Food Science and Preservation
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• v.16 no.5
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• pp.699-704
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• 2009

Commercially produced 'baby leaves' of Brassica campestris var. narinosa (Chinese cabbage) were used in the present study. Baby leaf vegetables were sown on 128 cell plug trays and harvested 30 days after sowing. For mechanical stress experiments, seedlings were thinned to three per cell, selected for uniformity, and watered at the base. Trays were treated with mechanical stress by stroking back and forth 50 times, using a sheet of A4 paper folded to double thickness. Plants were treated between 12:00 and 14:00 daily for 15-20 days. Harvested baby leaf vegetables were packed in MAP salad bags made of P-plus film, $50{\mu}m$ polypropylene (PP) film, and polyethylene terephthalate (PET) boxes. Fresh weight was well-maintained under P-plus and PP film on storage at $8^{\circ}C$. However, loss of fresh weight occurred quickly in PET boxes, and vegetable quality deteriorated rapidly. Stressed leaves were smaller but thicker, with an increased dry weight ratio. We thus suggest that P-plus or PP film is most appropriate packing for marketing of baby leaf vegetables, which should be stored at $8^{\circ}C$. Our data on baby leaf vegetables also make a significant new contribution in that we demonstrate a positive effect of stress touching on baby leaf processability.

• Journal of Life Science
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• v.26 no.8
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• pp.963-969
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• 2016

Kinesin superfamily proteins (KIFs) are microtubule-dependent molecular motor proteins essential for the intracellular transport of organelles and protein complexes in cells. Kinesin 1 is a member of those KIFs that transport various cargoes, including organelles, synaptic vesicles, neurotransmitter receptors, cell signaling molecules, and mRNAs through interaction between its light chain subunit and the cargoes. Kinesin light chains (KLCs) are non-motor subunits that associate with the kinesin heavy chain (KHC) dimer. KLCs interact with many different binding proteins, but their particular binding proteins have not yet been fully identified. We used the yeast two-hybrid assay to identify proteins that interact with the tetratricopeptide repeat (TPR) domain of KLC1. We found an interaction between the TPR domain of KLC1 and Wiskott-Aldrich syndrome protein family member 1 (WAVE1), a member of the WASP/WAVE family involved in regulation of actin cytoskeleton. WAVE1 bound to the six TPR domain-containing regions of KLC1 and did not interact with KHCs (KIF5A, KIF5B, and KIF5C) in the yeast two-hybrid assay. The carboxyl (C)-terminal verprolin-cofilin-acidic (VCA) domain of WAVE1 is essential for interaction with KLC1. Also, other WAVE isoforms (WAVE2 and WAVE3) interacted with KLC1 in the yeast two-hybrid assay. When co-expressed in HEK-293T cells, WAVE1 co-localized with KLC1 and co-immunoprecipitated with KLC1 and KIF5B. These results suggest that kinesin 1 motor protein may transport WAVE complexes or WAVE-coated cargoes in cells.

• Journal of Life Science
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• v.17 no.10
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• pp.1347-1353
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• 2007

The changes of glycoconjuagates (GCs) in rat kidney due to maturation were studied from samples of fetal and postnatal kidneys by lectin histochemistry. Rat kidneys of perinatal ages and adults were fixed in 4% paraformaldehyde and were stained with nine kinds of biotinylated lectins. The immature forms of the renal developmental stage such as vesicles and ureteric bud were observed in the cortex as late as day 14 of postnatal life, but the histological appearance of the weaning kidney was similar to that observed in adults. As for histochemical properties of GCs in the glomeruli, Con A affinity tended to increase with aging, but both RCA-1 and LCA affinities showed a transient increase in immature glomeruli of neonatal rats. DBA affinity with SBA, PNA, BSL-1 and RCA-1, additional Con A one in proximal tubule, were increased in both proximal and distal tubules according to maturation. In contrast to this, transient intensive LCA affinity were demonstrated in immature proximal and distal tubule of neonatal rats. In the collecting tubules, DBA, SBA, PNA and sWGA affinities tended to increase according to maturation, but transient increase for BSL-1, RCA-1 and LCA affinities were detected in neonatal rats. The present results suggest that the mature glycosylation pattern of the kidney undergoes profound changes during maturation and is probably associated with functional maturation of the kidney.