• Journal of Korean Medical classics
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• v.26 no.3
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• pp.53-73
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• 2013

Objective : This study will analyze the constitution of Danxihuqianwan(丹溪虎潛丸) with the dragon-tiger and lead-mercury theory of Su Shi(蘇軾). It would help understand more the usage of Danxihuqianwan over the scope of Sa-nam and bo-buk(瀉南補北) theory that has been well known until now. Methods : This study tried to analyze the explanation about Danxihuqianwan in Yixuerumen(醫學入門) that had used the dragon-tiger and lead-mercury concept with the theory of this kind that appears in Sushenliangfang(蘇沈良方). Results & Conclusion: Based on the internal alchemy theory, Su Shi proclaimed that dragon[龍] corresponds to mercury[汞] Jeong(精) Hyul(血), and tiger[虎] corresponds to lead[鉛] Qi(氣). It is said to be in good position that dragon appears from fire and tiger is hidden in water. Su Shi also expalined the relation between dragon and tiger as the relation of water[水] and fire[火] between heart and kidney. As Li Yan(李梴) did, the dragon-tiger theory could be applied to the analysis about Danxihuqianwan, and it explains well the effect of this prescription that makes tiger hidden in water and thus help cure the diseases such as atrophy syndrome.

• 한국균학회소식:학술대회논문집
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• 2015.05a
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• pp.60-60
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• 2015

Hybrid histidine kinase is a part of two-component system that is required for various stress responses and pathogenesis of pathogenic fungi. In the present study, Tco1, a homologue of human pathogen Cryptococcus neoformans Tco1 encoding a hybrid histidine kinase, was identified in corn smut pathogen Ustilago maydis by bioinformatic analysis. To explore the role of Tco1 in the virulence of U. maydis, mutants in which the tco1 gene was partially deleted were constructed by allelic exchange. The U. maydis tco1 mutants did show unaltered growth rate on axenic medium but were unable to produce conjugation tubes and develop fuzzy filaments, resulting in impaired mating of compatible strains. The expression levels of prf1, pra1, and mfa1 which are involved in the pheromone pathway significantly decreased in the tco1 mutants. In inoculation tests to host, the tco1 mutants showed significantly reduced ability in the production of anthocyanin pigments and tumor development on maize leaves. Overall, the combined results indicated that Tco1 plays important roles in sexual development and virulence of U. maydis by regulating the expression of the genes involved in the pheromone pathway.

• 한국균학회소식:학술대회논문집
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• 2015.05a
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• pp.29-29
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• 2015

Clubroot disease is caused by the soil-born obligate plant pathogen Plasmodiophora brassicae. This pathogen can infect all cruciferous vegetables and oil crops, including Brassica rapa, B. oleracea, B. napus, and other Brassica species. Clubroot disease is now considered to be a major problem in Chinese cabbage production in China, Korea, and Japan. We collected several hundreds of P. brassicae infected galls from Korea, and isolated the single spore from the collection. For establishment of novel isolation, and mass-propagation methods for singe spore isolates of P. brassicae pathogen, we developed new filtration method using both cellulose nitrate filter and syringe filter. Accurate detection of P. brassicae pathogen in the field was done by using real-time PCR in the potential infested soil. When we tested the different pathogenicity on commercial Chinese cabbage varieties, P. brassicae from collected galls showed various morphological patterns about clubroot symptom on roots. To date, 8 CR loci have been identified in the B. rapa genome using the quantitative trait loci (QTL) mapping approach, with different resistant sources and isolates. We are trying to develop the molecular marker systems for detect all 8 CR resistant genes. Especially for the study on the interaction between pathogens and CR loci which are not well understood until now, genome wide association studies are doing using the sequenced inbred lines of Chinese cabbage to detect the novel CR genes.

• 한국균학회소식:학술대회논문집
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• 2015.05a
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• pp.38-38
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• 2015

Throughout the world, clubroot disease is one of the most damaging diseases affecting Brassica oleracea. In order to perform QTL analysis of CR (clubroot resistance) loci in B. oleracea, we constructed a map, and analyzed CR-QTLs using the mean phenotypes of F3 progenies from the cross of a resistant double-haploid cabbage line (Anju) with a susceptible double-haploid broccoli line (GC). We identified one major QTL, pb-Bo(Anju)1 in C2 from Anju and four minor QTLs; pb-Bo(GC)1 in O5 from GC, pb-Bo(Anju)2, -3, -4 in C2, C3, and C7 from Anju, respectively. Additionally, we found that the accumulation of Pb-Bo(Anju)1 allele and the minor CR-QTLs is essential for resistance against various six isolates. Our finding markers closely linked to the CR-QTLs will help marker-assisted selection for CR. At present, we are undergoing toward map-based cloning for Pb-Bo(Anju)1 gene. The preliminary experiment delimited Pb-Bo(Anju)1 locus, encompassing among 450kB.

• 한국균학회소식:학술대회논문집
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• 2015.05a
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• pp.42-42
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• 2015

Plasmodiophora brassicae, the causal agent of clubroot disease, does the most serious damage to the Brassica crops. The limited control approaches make that the identification of clubroot resistance (CR) is more important for developing CR cultivars of the Brassica crops. So far, 8 CR loci were mapped. However, the variation of P. brassicae leads to the rapid erosion of its resistance. To identify novel CR genes, we employed three mapping population, derived from crosses between Chinese cabbage and turnip inbred lines ($59-1{\times}ECD04$ and $BJN3-1{\times}Siloga$) or between Chinese cabbage inbred lines ($BJN3-1{\times}85-I-II$), to perform QTL analysis. Totally, 8 CR loci were indentified and showed race-specific resistance. Physical mapping of these 8 loci suggested that 4 were located previously mapped position, indicating they might be the same allele or different alleles of the same genes. Other 4 loci were found to be novel. Further, CR near isogenic line carrying each CR locus was developed based on the marker assisted selection. Verification of these CR loci was underway. Identification of these novel CR genes would facilitate to breed broad-spectrum and durable CR cultivars of B. rapa by pyramiding strategies.

• 한국균학회소식:학술대회논문집
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• 2015.05a
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• pp.49-49
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• 2015

Over the past decade, clubroot has emerged as a major constraint to canola (Brassica napus) production in central Alberta, Canada. The number of fields with confirmed P. brassicae infestations in Alberta has increased steadily from 12 in 2003 to nearly 2,000 in 2014. Management of clubroot on canola has focused on sanitization of field equipment, soil amendments to reduce viable pathogen populations, long rotations out of susceptible crops and cropping of resistant cultivars. Clubroot resistance is the most effective and economical method of disease mitigation, but the recent identification of isolated P. brassicae populations with novel virulence phenotypes capable of overcoming resistance in most canola cultivars highlights the variable nature and adaptability of the pathogen. Recent studies have shown slight reductions in pathogen populations through crop rotations, but much more substantial reductions in spore populations in heavily infested areas near field entrances using fumigants such as Vapam (metam-sodium) or Basamid (dazomet). Greenhouse trials showed that seedling emergence, plant height and root weight increased, while primary and secondary infection and disease severity decreased with increased Basamid dosage. However, field trials showed some phytotoxicity. Application of Vapam at rates of 0.4 to $1.6mL\;L^{-1}$ soil resulted in 12-16 fold reductions in clubroot severity and primary and secondary infection. Vapam also was effective in reducing clubroot severity and improving canola seed yield under field conditions. These studies underscore the need for good resistance stewardship and for the integration of multiple products and practices for successful management of clubroot on canola.

• 한국균학회소식:학술대회논문집
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• 2015.05a
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• pp.54-54
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• 2015

Crops lack genetic resistance to most necrotrophic soil-borne pathogens and parasitic nematodes that are ubiquitous in agroecosystems worldwide. To overcome this disadvantage, plants recruit and nurture specific group of antagonistic microorganisms from the soil microbiome to defend their roots against pathogens and other pests. The best example of this microbe-based defense of roots is observed in disease-suppressive soils in which the suppressiveness is induced by continuously growing crops that are susceptible to a pathogen. Suppressive soils occur globally yet the microbial basis of most is still poorly described. Fusarium wilt, caused by Fusarium oxysporum f. sp. fragariae is a major disease of strawberry and is naturally suppressed in Korean fields that have undergone continuous strawberry monoculture. Here we show that members of the genus Streptomyces are the specific bacterial components of the microbiome responsible for the suppressiveness that controls Fusarium wilt of strawberry. Furthermore, genome sequencing revealed that Streptomyces griseus, which produces a novel thiopetide antibiotic, is the principal species involved in the suppressiveness. Finally, chemical-genetic studies demonstrated that S. griseus antagonizes F. oxysporum by interfering with fungal cell wall synthesis. An attack by F. oxysporum initiates a defensive "cry for help" by strawberry root and the mustering of microbial defenses led by Streptomyces. These results provide a model for future studies to elucidate the basis of microbially-based defense systems and soil suppressiveness from the field to the molecular level.

• 한국균학회소식:학술대회논문집
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• 2015.05a
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• pp.20-20
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• 2015

Endophytic fungi are microorganisms inhabiting living plant tissues without causing apparent harm to the host. They are drawing increasing attention due to their ability to produce various bioactive compounds as well as their effects on host growth and resistance to biotic and abiotic stresses. As a first step to assess biodiversity of plant associated fungi in Korea and the following evaluation on diverse biological activities, we are collecting endophytic fungi from plant in wild followed by systematic long-term storage in liquid nitrogen. Molecular identification using ITS sequences was also incorporated for pure culture by hyphal tip isolation. As of April 2015, about 1,400 fungal strains had been isolated from about 170 plant taxa. Fungal isolates belonging to Pleosporales, Diaporthales, Glomerellales, Hypocreales, and Xylariales were the most abundant. These collections are being used for several complementary researches, including screening of isolates with novel bioactive compounds or conferring drought stress resistance, phylogenetic and genomic study. Genome sequencing was performed for 3 isolates, one Xylaria sp. strain JS573 producing griseofulvin, an antifungal compound, and two Fusarium spp. strains JS626 and JS1030, which are assumed to be new species found in Korea. More detailed analysis on these genomes will be presented. These collections and genome informations will serve as invaluable resources for identifying novel bioactive materials in addition to expand our knowledge on fungal biodiversity.

• 한국균학회소식:학술대회논문집
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• 2014.05a
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• pp.39-39
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• 2014

In a homothallic filamentous fungus Aspergillus nidulans, sexual and asexual developments are largely affected by the genetic and environmental factors. To regulate the complex subsets of genes involved in the developmental processes accurately, tight regulations of transcription factors are required. The forkhead type transcription factors are the class of regulators that function in a broad spectrum of cellular and developmental processes in many species from yeast to human. Here, we identified the fkhA and fkhB genes that encode a conserved forkhead transcription factors. The fkhA deletion resulted in the complete loss of fruiting body formation under all conditions favoring sexual development, suggesting that the fkhA gene is required for sexual development in A. nidulans. Overexpression of fkhA resulted in enhanced formation of fruiting bodies under induction condition not only in the normal condition but also in the condition of presence of 0.6 M KCl, which strongly inhibits sexual development. To know the function of the fkhB gene, we also generated fkhB knock-out strain in A. nidulans. Deletion of fkhB resulted in abnormal conidiophore formation under standard conditions and delayed sexual development process, suggesting that the fkhB gene plays an important role in conidiophore morphogenesis Taken together, these results suggest that the fkhA gene is necessary and sufficient for regulating sexual development and the fkhB gene is a transcription factor related in asexual developmental process in A. nidulans.

• 한국벤처창업학회:학술대회논문집
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• 2017.04a
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• pp.36-36
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• 2017

기업 간, 브랜드 간의 치열한 경쟁은 날이 갈수록 심해지고 있으며, 좋은 제품을 만들 수 있는 기술력이나 우수한 인력, 양호한 재무능력은 기업이 생존하기 위해 기본적으로 갖추어야 할 필요조건이 되었다. 그런데 기업이 성공하기 위해서는 어떤 능력이 필요할까? 바로 마케팅능력이다. 마케팅 능력이 있어야 충성도 있는 고객을 창출할 수 있고 시장점유율 제고를 통해서 목표하는 바람직한 성과를 산출할 수 있다. 마케팅 부문의 능력이나 지식은 타 부문에 비해서 기업성과에 미치는 영향이 크며, 궁극적으로는 기업의 지속적 경쟁우위를 달성하기 위하여 반드시 갖추어야 할 요소라는 것을 많은 연구들은 보여주고 있다. 이때 기업이나 브랜드가 윤리적인 모습을 소비자에게 보여준다면 경쟁자에 비해서 훨씬 용이하게 충성도를 이끌어 낼 수 있을 것이다. 따라서 마케팅윤리는 매우 중요한 이슈이다. 최근 소비자의 감정을 상하게 하는 국내외 기업행태가 매스컴에 자주 오르내리고 있는데, 이러한 소식을 접한 소비자들은 해당기업에 대해서 부정적인 인식을 가지게 되어 제품이나 서비스를 구매하지 않으며, 주변사람들에게 좋지 않은 입소문을 내기도 하므로 기업의 마케팅 담당자는 윤리적인 측면을 고려하여 의사결정을 내려야한다. 기업의 사회적 책임(CSR)과 마케팅윤리는 상호 연관된 개념으로 보아도 무방하다. 그런데 마케팅의 윤리적인 측면을 고려하다보면 의사결정 상황이 마케팅 분야에만 국한되지 않거나 소비자의 이익과 기업의 이익이 상충하는 경우가 빈번하게 발생하게 되어 의사결정을 어렵게 만든다. 특히, 스타트업의 경우는 기존기업에 비해서 업력이 길지 않고 우선적으로 처리해야하는 업무가 많다보니 마케팅 윤리적 측면을 중요하게 생각하지 않는 경향이 많다. 하지만 마케팅 윤리는 단시간에 구축하거나 보완할 수 있는 것이 아니므로 스타트업의 경영자들은 이 문제를 중요하게 생각하여야 할 것이다. 본 연구는 기존의 마케팅 윤리연구 동향을 분석하여 스타트업의 마케팅윤리 연구의 방향을 제시하고자 한다.