• Title/Summary/Keyword: 센서 DNA

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Biosensors: a review (바이오센서)

  • Hwang, Kyo-Seon;Kim, Sang-Kyung;Kim, Tae-Song
    • Journal of Sensor Science and Technology
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    • v.18 no.4
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    • pp.251-262
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    • 2009
  • Biosensors exploit the specific binding between recognition molecule on the biosensor surface and target molecule in analyte and are used in the detection of specific biomolecules such as protein, DNA, cell, virus, etc., with a view towards developing analytical devices. Recently, application field of biosensors have been expanding from diagnosis to biodefense because they can basically serve as high performance devices. This review describes the basic information of biosensors including definition, classification, and operational principle. Moreover, we introduce micro/nano technology-based biosensors with better detection performance than traditional method and their application examples.

Fabrication of a polymerase chain reaction micro-reactor using infrared heating

  • Im, Ki-Sik;Eun, Duk-Soo;Kong, Seong-Ho;Shin, Jang-Kyoo;Lee, Jong-Hyun
    • Journal of Sensor Science and Technology
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    • v.14 no.5
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    • pp.337-342
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    • 2005
  • A silicon-based micro-reactor to amplify small amount of deoxyribonucleic acid (DNA) has been fabricated using micro-electro-mechanical systems (MEMS) technology. Polymerase chain reaction (PCR) of DNA requires a precise and rapid temperature control. A Pt sensor is integrated directly in the chamber for real-time temperature measurement and an infrared lamp is used as external heating source for non-contact and rapid heating. In addition to the real-time temperature sensing, PCR needs a rapid thermocycling for effective PCR. For a fast thermal response, the thermal mass of the reactor chamber is minimized by removal of bulk silicon volume around the reactor using double-side KOH etching. The transparent optical property of silicon in the infrared wavelength range provides an efficient absorption of thermal energy into the reacting sample without being absorbed by silicon reactor chamber. It is confirmed that the fabricated micro-reactor could be heated up in less than 30 sec to the denaturation temperature by the external infrared lamp and cooled down in 30 sec to the annealing temperature by passive cooling.

Development of Microfluidic Chip for Enrichment and DNA Extraction of Bacteria Using Concanavalin A Coated Magnetic Particles (Concanavalin A가 코팅 된 자성 입자를 이용한 미생물 농축 및 유전자 추출 칩 개발)

  • Kwon, Kirok;Gwak, Hogyeong;Hyun, Kyung-A;Jung, Hyo-Il
    • Journal of Sensor Science and Technology
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    • v.27 no.4
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    • pp.237-241
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    • 2018
  • The real-time enrichment and detection of pathogens are serious issues and rapidly evolving field of research because of the ability of these pathogens to cause infectious diseases. In general, bacterial detection is accomplished by conventional colony counting or by polymerase chain reaction (PCR) after DNA extraction. As colony counting requires considerable time to cultivate, PCR is an attractive method for rapid detection. A small number of pathogens can cause diseases. Hence, a pretreatment process, such as enrichment is essential for detecting bacteria in an actual environment. Thus, in this study, we developed a microfluidic chip capable of performing rapid enrichment of bacteria and the extraction of their genes. A lectin, i.e., Concanavalin A (ConA), which shows binding affinity to the surface of most bacteria, was coated on the surface of magnetic particles to nonspecifically capture bacteria. It was subsequently concentrated through magnetic forces in a microfluidic channel. To lyse the captured bacteria, magnetic particles were irradiated by a wavelength of 532nm. The photo-thermal effect on the particles was sufficient for extracting DNA, which was consequently utilized for the identification of bacteria. Our device will help monitor the existence of bacteria in various environmental situations such as water, air, and soil.

Single Interaction Force of Biomolecules Measured with Picoforce AFM (원자 힘 현미경을 이용한 단일 생분자 힘 측정)

  • Jung, Yu-Jin;Park, Joon-Won
    • Journal of the Korean Vacuum Society
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    • v.16 no.1
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    • pp.52-57
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    • 2007
  • The interaction force between biomolecules(DNA-DNA, antigen-antibody, ligand-receptor, protein-protein) defines not only biomolecular function, but also their mechanical properties and hence bio-sensor. Atomic force microscopy(AFM) is nowadays frequently applied to determine interaction forces between biological molecules and biomolecular force measurements, obtained for example using AFM can provide valuable molecular-level information on the interactions between biomolecules. A proper modification of an AFM tip and/or a substrate with biomolecules permits the direct measurement of intermolecular interactions, such as DNA-DNA, protein-protein, and ligand-receptor, etc. and a microcantilever-based sensor appeared as a promising approach for ultra sensitive detection of biomolecular interactions.

Development of DNA Hybridization Detection Sensors and Analysis of Characteristics Using Electrochemical methods (전기화학법을 이용한 DNA Hybridization 검출 센서의 개발 및 특성 해석)

  • Ock, Jin-Young;Kim, Do-Kyun;Chang, Jeong-Soo;Kwon, Young-Soo
    • Proceedings of the KIEE Conference
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    • 2002.11a
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    • pp.260-262
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    • 2002
  • The determination of DNA hybridization can apply the molecular biology research. clinic diagnostics. bioengineering, environment monitoring, food science and other application area. So, The determination of hybridization is very important for the improvement of DNA detection system. In this study, we report the characterization of the DNA hybridization by the electricalchemical methods. The probe oligonucleotide was used to determine the amount of target oligonucleotide in solution using Methylen Blue(MB) as the electrochemical indicators. The cathodic peak currents($I_{peak}$) of MB were linearly related to the concentration of the target oligonucleotide sequence in the range $1[{\mu}M]{\sim}0.1[{\mu}M]$. The detection limit of this approach was 0.01[nM]. As a result, the match oligonucleotide(CR-1) was most stable state and the peak of redox current measured by DNA hybridization detection sensors by using electrochemical method seem to be similar to 1-mer terminal mismatch oligonucleotide(MR-3). The MR-2, MR-3, MR-22 and MR-33 have each mismatching sequence of central and terminal. With this set the role of point mutations was to be investigated. Terminal mismatch oligonucleotide (MR-3, 33) is shown more stable state than central mismatch oligonucleotide(MR-2, 22). And 1-mer mismatch oligonucleotide(MR-2 or 3) is shown more stable state than 2-mer mismatch oligonucleotide(MR-22 or 33).

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Biosensors (바이오센서)

  • 김의락
    • KSBB Journal
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    • v.15 no.5
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    • pp.423-427
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    • 2000
  • Intense research on biosensors has been performed in a number of different institution over the past 15 years, but relatively few commercial products have resultingly, the blood glucose sensor is a good example of a product which penetrated the market. However recently, the development of electrochemical and optical technologies has accelerated the turnover of the research as is illustrated by a rapid increase in the number of point-of-care diagnostic systems and analytical devices. Examples of such biosensors used in the fields of medical diagnostics, bioprocess control, and environmental monitoring are described, and summarized in an introduction to their characteristics, structures, and functions, given.

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Development of the Filterable Water Sampler System for eDNA Filtering and Performance Evaluation of the System through eDNA Monitoring at Catchment Conduit Intake-Reservoir (eDNA 포집용 채수 필터시스템 개발과 집수매거 취수지 내에서의 성능평가)

  • Kwak, Tae-Soo;Kim, Won-Seok;Lee, Sun Ho;Kwak, Ihn-Sil
    • Korean Journal of Ecology and Environment
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    • v.54 no.4
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    • pp.272-279
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    • 2021
  • A pump-type eDNA filtering system that can control voltage and hydraulic pressure respectively has been developed, and applied a filter case that can filter out without damaging the filter. The filtering performance of the developed system was evaluated by comparing the eDNA concentration with the conventional vacuum-pressured filtering method at the catchment conduit intake reservoir. The developed system was divided into a voltage control (manual pump system) method and a pressure control (automatic pump system) method, and the pressure was measured during filtering and the pressure change of each system was compared. The voltage control method started with 65 [KPa] at the beginning of the filtering, and as the filtering time elapsed, the amount of filtrate accumulated in the filter increased, so the pressure gradually increased. As a result of controlling the pressure control method to maintain a constant pressure according to the designed algorithm, there was a difference in the width of the hydraulic pressure fluctuation during the filtering process according to the feedback time of the hydraulic pressure sensor, and it was confirmed that the pressure was converged to the target pressure. The filtering performance of the developed system was confirmed by measuring the eDNA concentration and comparing the voltage control method and the hydraulic control method with the control group. The voltage control method obtained similar results to the control group, but the hydraulic control method showed lower results than the control group. It is considered that the low eDNA concentration in the hydraulic control method is due to the large pressure deviation during filtering and maintaining a constant pressure during the filtering process. Therefore, rather than maintaining a constant pressure during filtering, it was confirmed that a voltage control method in which the pressure is gradually increased as the filtrate increases with the lapse of filtering time is suitable for collecting eDNA. As a result of comparing the average concentration of eDNA in lentic zone and lotic zone as a control group, it was found to be 96.2 [ng µL-1] and 88.4 [ng µL-1l], respectively. The result of comparing the average concentration of eDNA by the pump method was also high in the lentic zone sample as 90.7 [ng µL-1] and 74.8 [ng µL-1] in the lentic zone and the lotic zone, respectively. The high eDNA concentration in the lentic zone is thought to be due to the influence of microorganisms including the remaining eDNA.

바이오 센서 및 랩온어칩

  • 박유근
    • The Magazine of the IEIE
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    • v.31 no.1
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    • pp.58-72
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    • 2004
  • Smart sensors and biochip technologies have received a great deal of attention in recent years not only because of the enormous potential markets in the healthcare expenditures but more importantly because of its great impact on the quality of human life in the future. Collaborative research among BT (Bio Technologies), IT (Information Technologies) and NT (Nano Technologies) will bring us a new paradigm of the healthcare services. Examples include disease prediction based on the genetic tests, personal medicines, point-of-care analysis, rapid and sensitive infectious disease diagnostics, environmental monitoring for chemical or biological warfares, intelligent drug delivery systems etc. In this report, recent accomplishment in the research area on biosensors, DNA chips, Protein Chips and Lab-on-a-chips are reviewed.

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Introduction to research and current trend about nanopore-based nanobiosensor (나노포어 기반 나노바이어센서 기술)

  • Kim, Joo Hyoung;Youn, Yeoan;Lee, Choongman;Yoo, Kyung-Hwa
    • Vacuum Magazine
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    • v.2 no.1
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    • pp.4-9
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    • 2015
  • A nanopore is a very small hole that can be used as single-molecule detector. The detection principle is based on monitoring the ionic current reduced by passage of a molecule through the nanopore as a voltage is applied across the nanopore. Here, we introduce biological nanopores and solid-state nanopores. Then, research and current trend about nanopore-based DNA biosensor and protein analysis are reviewed.

Surface Acoustic Wave Characteristics of Piezoelectric Materials and Protein Immobilization (압전 재료의 탄성표면파 특성과 단백질의 고정화)

  • Chong, Woo-Suk;Hong, Chul-Un;Kim, Gi-Beum
    • Korean Chemical Engineering Research
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    • v.44 no.2
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    • pp.166-171
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    • 2006
  • In this study, in using a piezoelectric material of $Pb(Mg_{1/3}Nb_{2/3})O_3-PbTiO_3$ (PMN-PT), which has a high electromechanical coupling coefficient, we have tried to study about this material can be practically available as a new biosensor to detect protein by using surface acoustic wave (SAW). As the results, the filtering of the center frequency of the PMN-PT substrate is a superior result to that of the $LiTaO_3$ (LT) substrate, but the result was not completely satisfactory. Also this study attempts to develop a sensing method to detect mismatched DNA in order to diagnose cancer. We could directly immobilize the MutS to the NTA using the EDC solution. But, we immobilized MutS using nickel and it is judged that is more effective method to detect mismatched DNA.