• Korean Journal of Microbiology
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• v.46 no.1
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• pp.33-37
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• 2010

Rhizobacterial strain isolated from barren soil, Bacillus subtilis RFO41 exhibits a high level of phosphate solubilizing activity and produces some phytohormones. Its promoting effect on the growth of Xanthium italicum Moore, a wild plant growing at lakeside barren land and thus a good candidate plant for revegetation of barren lakeside was evaluated in the in situ test for 19 weeks at Lake Paro, Kangwon-do. Strain RFO41 could enhance the dry weight of X. italicum by 67.7%. It also increased the shoot length of X. italicum plant by 21.1% compared to that of uninoculated control. Both growth enhancements had statistical significance. However, the inoculation did not show any effect on the root growth, which might be due to the breakage of tiny root. Denaturing gradient gel electrophoresis analysis showed that the inoculated bacteria were maintained in the soils, and the indigenous bacterial community did not exhibit any significant change. This plant growth promoting capability may be utilized as an environment-friendly and low cost revegetation method, especially for the sensitive areas such as barren lakeside lands.

• Journal of the Korean Society of Tobacco Science
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• v.2 no.2
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• pp.20-37
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• 1980

Among the 34 strains of Nicotinophiles selected in the previous experiments, strain NCT27 identified with Pseudomonas putida and strain NCT30 identified with Arthrobacter oxydans biotype nan thus were Investigated for optimization of growth conditions for nicotine degradation and other cultural characteristics. The compositions of optimized medium were to be following: $KH_2PO_4$ 2.Ogr, KCI 5.Ogr, $MgSO_4$.$7H_2O$ 20mg, $MnSO_4$.$6H_2O$ 0.2mg, $FeSO_4$.$7H_2O$ 1.Omg, Col$^{++}$ (Cobalt Acetate),2.O$\gamma$, N1$^{++}$ (NiSO4,6H2O) 0.5$\gamma$, and yeast extract 80mg per liter. The optimum initial concentrations of nicotine for growth were 0.4% for Pseudomonas and 0.1% for Arthrobacter, respectively. The optimum temperature and pH were 3$0^{\circ}C$ and 7.0 for both of strains. The pH of culture medium of Pseudomonas was changed from acidic condition to basic one in going from the logarithmic growth phase to the stationary growth phase. In contrast with Pseudomonas, it remained constant in case of Arthrobacter. The growth of Arthrobacter was completely inhibited in the nicotine concentration of 0.7&. However, Pseudomonas could grow even in the nicotine concentration of 1.0%. Moreover, it could grow successfully in the tobacco extract media as well as media containing carbon and nitrogen sources other than nicotine. The maximum rates of nicotine degradation were to be 1.22 gr./hr./liter for Pseudomonas and 0.186 gr./hr./liter for Arthrobacter, respectively.

• The Korean Journal of Mycology
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• v.24 no.1 s.76
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• pp.17-24
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• 1996

For screening of biological activities of culture broth of some wood rotting basidiomycetes, antimicrobial activity, plant growth regulating activity, antitumor activity, and various enzyme activities were checked. Coriolus versicolor 5129 and C. pubescens 5131 strains showed inhibition activity against gram-positive bacteria and Lenzites betulina 8029 strain showed the activity against gram-negative bacteria. L. betulina 8085 inhibited the growth of both bacteria and plant pathogenic fungi. All of tested basidiomycetes inhibited the germination and growth of radish and cabbage at concentration of 0.8ml/ml. Especially, Fomitopsis pinicolor 8059 and Fomitella fraxinea 8084 showed strong inhibition activity. In contrast, Bjerkandera adusta 8054 stimulated the growth of cabbage and radish at concentration of 0.4 and 0.2ml/ml. All polysaccharides from tested basidiomycetes showed anti-tumor activity against sarcoma 180 and the stronger antitumor activity was observed in L. betulina 8029 and unidentified 8058 strain. All tested basidiomycetes had also an ability to degrade cellulose and lignin.

• KSBB Journal
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• v.20 no.5 s.94
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• pp.371-375
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• 2005

In the course of our search for compounds effective to multidrug-resistant cancer cells from myxobacteria with the adriamycin-resistant cancer cell line CL02, we found cytotoxic activity against the CL02 cells in culture extract of Sorangium cellulosum JW1045. Activity-guided fractionation of the culture extract led to the isolation of an active principle, chivosazole F, This compound showed high cytotoxic activity against cultured human cancer cells. The $IC_{50}$ values, measured by a SRB assay with different cell lines, ranged from 0.1 to 10 ng/ml. Furthermore chivosazole F was as active against drug-resistant cancer cells CL02 and CP70 as against the corresponding sensitive cells.

• Journal of Life Science
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• v.14 no.1
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• pp.163-166
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• 2004

To investigate the bacterial growth on granular activated carbon (GAC) for the purification of tap water, fixed bed columns with GAC were installed and operated at an empty bed contact time (EBCT) of 1 min$\pm$0.08 min. There was no bacterial breakthrough in the spring. However, the bacterial concentrations of effluent (($10^3$ CFU/ml) were higher than that of the influent ($10^2$ CFU/ml) after 10 day operation in summer. More bacteria were enumerated near the entering point of the tap water, while the bacterial activities were similar throughout the columns. Different bacterial species were detected on coal- and plant-based GAC, although the dominant genus was the same as Acinetobacter.

• Proceedings of the Korean Society for Applied Microbiology Conference
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• 1975.12a
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• pp.181.3-181
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• 1975

서울주변 78지역의 토양과 하수를 수집하여 200ppm의 카드뮴을 함유한 배지에서 생장하는 142주의 세균류를 분리하였다 이들중 1,500ppm의 카드뮴함유 배지에서 증식하는 8주를 선발하고 그 중 1주에 대하여 100 및 500ppm의 카드뮴 함유배지에서의 생장곡선을 얻었다. 또한 0.5, 1, 10및 100ppm의 카드뮴함유 배지에서 배양시간에 따른 균체내 카드뮴의 축적을을 조사한바 0.5ppm에서 최고 59％이였다.(중략)

• Korean Journal Plant Pathology
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• v.12 no.2
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• pp.197-201
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• 1996

우리나라 포도재배 지역인 포천, 남양주 및 수원의 포도나무 줄기혹병에 이병된 포장으로부터 채집한 줄기혹(gall), 수액(sap) 및 근권토양으로부터 56균주의 유사 Agrobacterium를 분리하였으며, 이들 중 7균주가 포도나무 유묘의 줄기에 혹ㅇ르 형성시키는 병원성 Agrobacterium biovar의 선택배지에서의 생장, oxidase의 생성, Na L-tartrate로부터 알칼리의 성장, 2% NaCl 첨가 배지에서 생장, 3-ketolactose의 비생산, mesoerythritol과 ethanol로부터 산을 생성하지 못하는 등 Agrobacterium biovar 3인 A. vitis로 동정되었다.

• Journal of Food Hygiene and Safety
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• v.33 no.3
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• pp.157-161
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• 2018

The objective of this study was to develop software to predict the kinetic behavior and the probability of foodborne bacterial growth on processed meat products. It is designed for rapid application by non-specialists in predictive microbiology. The software, named Foodborne bacteria Animal product Modeling Equipment (FAME), was developed using Javascript and HTML. FAME consists of a kinetic model and a probabilistic model, and it can be used to predict bacterial growth pattern and probability. In addition, validation and editing of model equation are available in FAME. The data used by the software were constructed with 5,400 frankfurter samples for the kinetic model and 345,600 samples for the probabilistic model using a variety of combinations including atmospheric conditions, temperature, NaCl concentrations and $NaNO_2$ concentrations. Using FAME, users can select the concentrations of NaCl and $NaNO_2$ meat products as well as storage conditions (atmosphere and temperature). The software displays bacterial growth patterns and growth probabilities, which facilitate the determination of optimal safety conditions for meat products. FAME is useful in predicting bacterial kinetic behavior and growth probability, especially for quick application, and is designed for use by non-specialists in predictive microbiology.

• Journal of Food Hygiene and Safety
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• v.28 no.3
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• pp.217-221
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• 2013

This study developed predictive models for the kinetic behavior of Staphylococcus aureus on processed cheeses. Mozzarella slice cheese and cheddar slice cheese were inoculated with 0.1 ml of a S. aureus strain mixture (ATCC13565, ATCC14458, ATCC23235, ATCC27664, and NCCP10826). The inoculated samples were then stored at $4^{\circ}C$ (1440 h), $15^{\circ}C$ (288 h), $25^{\circ}C$ (72 h), and $30^{\circ}C$ (48 h), and the growth of all bacteria and of S. aureus were enumerated on tryptic soy agar and mannitol salt agar, respectively. The Baranyi model was fitted to the growth data of S. aureus to calculate growth rate (${\mu}_{max}$; ${\log}CFU{\cdot}g^{-1}{\cdot}h^{-1}$), lag phase duration (LPD; h), lower asymptote (log CFU/g), and upper asymptote (log CFU/g). The growth parameters were further analyzed using the square root model as a function of temperature. The model performance was validated with observed data, and the root mean square error (RMSE) was calculated. At $4^{\circ}C$, S. aureus cell growth was not observed on either processed cheese, but S. aureus growth on the mozzarella and cheddar cheeses was observed at $15^{\circ}C$, $25^{\circ}C$, and $30^{\circ}C$. The ${\mu}_{max}$ values increased, but LPD values decreased as storage temperature increased. In addition, the developed models showed acceptable performance (RMSE = 0.3500-0.5344). This result indicates that the developed kinetic model should be useful in describing the growth pattern of S. aureus in processed cheeses.

• Journal of Food Hygiene and Safety
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• v.38 no.6
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• pp.528-536
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• 2023

In Korea, from January 2023, the Act on Labeling and Advertising of Food was revised to reflect the use-by-date rather than the sell-by-date. Hence, the purpose of this study was to establish a system for calculating the safety factor and determining the recommended use-by-date for each food type, thereby providing a scientific basis for the recommended use-by-date labels. A safety factor calculation technique based on scientific principles was designed through literature review and simulation, and opinions were collected by conducting surveys and discussions including industry and academia, among others. The main considerations in this study were pH, Aw, sterilization, preservatives, packaging for storage improvement, storage temperature, and other external factors. A safety factor of 0.97 was exceptionally applied for frozen products and 1.0 for sterilized products. In addition, a between-sample error value of 0.08 was applied to factors related to product and experimental design. This study suggests that clearly providing a safe use-by-date will help reduce food waste and contribute to carbon neutrality.