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Molecular biological studies on Heat-Shock Responses in Amoeba proteus: I. Detection of Heat-shock Proteins (아메바(Amoebaproteus)의 열충격 대응에 관한 분자생물학적 연구: 1 . 열충격 대응 단백질의 탐색)

  • 홍혜경;최지영안태인
    • The Korean Journal of Zoology
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    • v.37 no.4
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    • pp.554-564
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    • 1994
  • 세균이 세포내 공생하는 xD strain과 모 세포주인 tD strain Amoeba proteus의 열충격 대응의 차이를 알아 보기 위하여 방사선 동위원소로 표지된 아미노산을 Ca2+_less Chalkley's 용액에서 음작용 경로를 통하여 90분 동안 흡수하게 하고, 저온 및 고온 스트레스에 대하여 새로 합성되는 스트레스 대응 단백질의 양상을 1, 2차원 전기영동 및 자기방사 사진법에 의해서 비교하였다 저온(10"C) 충격에 대응하여 아메바는 두 strain 모두 56.0 kDa, pl 6.0 단백질을 강하게 발현하였으며, xD strain에서는 tD strain과 달리 저온 충격 초기에 66 0 kDa, pl 5.5 단백질의 발현이 중단되었다. 한편 고온(33"C) 열충격에 대하여 두 strain 아메바에서 모두 10여종의 단백질이 새합성되는 것으로 확인되었으며, tD 아메바에는 이들 단백질의 새합성이 완만하게 이루어지는데 비하여 xD 아메바에서는 그중 66.0 kDa 단백질이 고온 대응 단백질로서 신속하게 새합성되는 것으로 나타났다. 이외에도 2차원 전기 영동 분석을 통하여 열충격에 의해서 발현이 촉진되는 다수의 단백질들을 탐지하였다 탐지된 아메바의 열충격 단백질은 분자량에 따라 hsp100군 2종, hsp90군, 3종, hsp70군 및 hsp60군 각 1종, 그리고 small csp군 4종으로 분류해 볼 수 있었다 두 분석의 결과를 종합해 보면 tD 아메바에는 저온 및 고온 충격에 대하여 열충격 단백질의 합성이 완만하게 상승하는 데 비하여 xD strain에서는 신속하게 이루어졌다. 이상의 결과로 보아 아메바의 세포내 공생 세균은 숙주의 열충격 대응기작에 변화를 야기한 것으로 판단된다한 것으로 판단된다. 10mg과 20mg의 estrogen 처리구 사이에 유두 직경, 길이 그리고 용적의 증가량에 있어서는 차이가 없었다. 10mg 및 20mg의 estrogen 처리는 초발정일령을 각각 20일 및 124일 단축시켰다. 전체적으로 이러한 결과는 송아지에 estradiol의 삽입은 성장과 유선 발달을 촉진시키고 초발정일령을 단축시킬수 있다는 것을 강력하게 지적한다. 일치하지 않으므로 더욱 정밀한 조사를 실시하여 분류학상의 위치를 정확히 밝혀 볼 필요가 있을 것으로 생각되었다.연한 도구이자 정신활동으로 보게함으로써, 주제 및 연구방법에서 획일성보다 다양성과 창조성이 강조되고 있다. 그리고 연구에 있어서 주제 의 다양성을 통해 보다 현실생활에 밀접하게 연결되어야 할 필요성은 학문이나 과학의 사회 성에 대한 새로운 인식을 가져다 주고 있다. 이러한 지리교육과정의 좌표의 변화된 측면들 을 고려하여, 지리교육과정의 새로운 방향은 다음의 세가지로 모색될 수 있다. 첫째, 爭點中 心 地理敎育課程이다. 사회쟁점에 대한 접근은 쟁점의 이해와 문제해결에의 지리적 관점의 활용을 통해 학습내용의 시사성과 사실성을 높힐 수 있다. 이때 문제해결능력을 통해 현대 시민의 자질 및 능력을 기를 수 있음은 물론, 다른 한편으로 실제세계 즉 학생의 실생활, 사 회, 국가, 세계에서 일어나는 일들과의 관련성을 갖게 함으로써, 내적 동기화와 외적인 자극 을 강력하게 결합할 수 있을 것이다. 이는 개인적 유관적합성과 사회적 유관적합성을 동시 에 확보하는 데 유리할 것이다. 둘째, 思考中心 地理敎育課程이다. 지리교육은 학생들을 지 식 및 기능의 숙달자가 되도록 할 것이 아니라 기본적 문장해독력의 수준을 넘어 능력있는 사고자로 길러내는 것을 목표로 하여야 한다.

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Biodiversity Conservation and the Yellow Sea Large Marine Ecosystem Project (생물다양성 보전과 황해 광역 해양생태계 관리계획)

  • Walton, Mark
    • Journal of the Korean Society for Marine Environment & Energy
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    • v.13 no.4
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    • pp.335-340
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    • 2010
  • The paper describes the objectives of Yellow Sea Large Marine Ecosystem (YSLME) project, focusing on procedural and practical aspects. YSLME is a highly productive sea yet possibly one of the most impacted large marine ecosystems, in terms of anthropogenic stressors, due the enormous coastal population. The aim of the YSLME project is the reduction of ecosystem stress through identification of the environmental problems in the Transboundary Diagnostic Analysis (TDA) that are then addressed in the Strategic Action Programme (SAP). One of the major problems found to be affecting biological diversity is habitat modification through wetland reclamation, conversion and degradation. Since the early 1900's more than 40% of intertidal wetlands have been reclaimed in Korea, and 60% of Chinese coastal wetlands have been converted or reclaimed. Damaging fishing practices, pollution and coastal eutrophication have further degraded the coastal environment reducing the biological diversity. To combat this loss, the YSLME project has mounted a public awareness campaign to raise environmental consciousness targeted at all different levels of society, from politicians at parliamentary workshops, local government officer training events, scientific conferences and involvement of scientists in the project research and reporting, to university and high school students in our visiting internship programmes and environmental camps. We have also built networks through the Yellow Sea Partnership and by liaising and working with other environmental organizations and NGOs. NGO's are recognised as important partners in the environmental conservation as they already have extensive local networks that can be lacking in international organisations. Effective links have been built with many of these NGOs through the small grants programme. Working with WWF's YSESP project and other academic and research institutions we have conducted our own biodiversity assessments that have contributed to the science-based development of the SAP for the YSLME. Our regional targets for biodiversity outlined in the SAP include: Improvements in the densities, distributions and genetic diversity of current populations of all living organisms including endangered and endemic species; Maintenance of habitats according to standards and regulations of 2007; and a reduction in the risk of introduced species. Endorsement of the SAP and its successful implementation, during the proposed second phase of the YSLEM project, will ensure that biological diversity is here to benefit future generations.

Ngugi wa Thiong'o's Pan-Africanism: People's Memory and Alliance to Overcome Postcolonial Nations (응구기와 시옹오의 범아프리카주의 - 포스트식민 국가를 넘어서는 주변부의 기억과 연대)

  • Lee, Hyoseok
    • Cross-Cultural Studies
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    • v.42
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    • pp.107-129
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    • 2016
  • In modern history, there have been several kinds of continental unions or supranational politico-economic unions in the world, such as the United Nations, the European Union, the Union of South American Nations, the African Union, etc. Modern thinkers proposed many pan-isms on their continental base, for example, Pan-Arabism, Pan-Latin Americanism, Pan-Asianism, Pan-Celtism, etc. What is the most common in these pan-isms is that a continental union would be a politico-economic system to overcome the limits of the modern state-nation and to realize a long and happy relationship between member nations and continents. However, the concept of a supranational union differs from that of cosmopolitanism, in that the former presupposes the common cultural and historical heritage in the concerned region or continent. Ngugi wa Thinog'o' Pan-Africanism implies two keywords that are connected to his concepts such as 'decentralization' and 'African languages.' Pan-Africanism supposes that Africa may gain benefits from the union of African nations under the umbrella of anti-colonial efforts to down size the Euro-American influences. Moreover, using African languages enhances self-reliance and self-imagination among the African people. For in the former colonial regimes, the European colonial languages, such as English, French, or Portuguese, were central to the dissemination of European culture and modernity. Ngugi asserts that the African peripheralized languages could reinstate the African cultural heritage and propose an alternative to the Western modernity.

Report for Spreading Culture of Medical Radiation Safety in Korea : Mainly the Activities of the Korean Alliance for Radiation Safety and Culture in Medicine(KARSM) (국내 의료 방사선 안전문화 활동 현황 : 의료방사선안전문화연합회 중심으로)

  • Yoon, Yong-Su;Kim, Jung-Min;Kim, Hyun-Ji;Choi, In-Seok;Sung, Dong-Wook;Do, Kyung-Hyun;Jung, Seung-Eun;Kim, Hyung-Soo
    • Journal of radiological science and technology
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    • v.36 no.3
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    • pp.193-200
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    • 2013
  • There are many concerns about radiation exposure in Korea after Fukushima Nuclear Plant Accident on 2011 in Japan. As some isotope materials are detected in Korea, people get worried about the radioactive material. In addition, the mass media create an air of anxiety that jump on the people's fear instead of scientific approach. Therefore, for curbing this flow, health, medical institute from the world provide a variety of information about medical radiation safety and hold the campaign which can give people the image that medical radiation is safe. At this, the Korean Food and Drug Administration(KFDA) suggested that make the alliance of medical radiation safety and culture on August, 2011. Seven societies and institutions related medical radiation started to research and advertise the culture of medical radiation safety in Korea. In this report, mainly introduce the activities of the Korean Alliance for Radiation Safety and Culture in Medicine(KARSM) for spreading culture of medical radiation safety from 2011 to 2012.

A study on the current status and improvement suggestions of cultural heritage education in primary school Focused on curriculum analysis and teachers' cultural heritage education experiences (초등학교 문화유산 교육 현황과 방향 제언 - 교육 과정 분석 및 교사의 문화유산 교육 경험을 중심으로 -)

  • HAN, Geonsoo;KIM, Dawon
    • Korean Journal of Heritage: History & Science
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    • v.54 no.4
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    • pp.132-151
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    • 2021
  • The purpose of this study is to analyze the current status of and suggest future improvement practices for cultural heritage education in primary school. We analyzed a primary school textbook for each of five subjects (Korean, social studies, music, art, physical education), surveyed ten teachers with cultural heritage education experience, and analyzed the results. Based on the results, we made the following five suggestions: First, the goal of cultural heritage education should be expanded to foster citizenship. Second, in cultural heritage education, it is necessary to discuss what should be included in the educational content and to organize the content systematically in connection with the student environment, local community environment, and subject content. Third, cultural heritage education should be linked with sustainable development so that cultural heritage can be passed on from the present generation to future generations. Cultural heritage has characteristics, such as universal values of humanity, that transcend national boundaries, regional identities, dialogue and understanding between civilizations, and sources of creativity, and thus serves as the foundation for sustainable development. Fourth, for the effective implementation of cultural heritage education in schools, it is necessary to develop step-by-step teacher training programs. The success or failure of cultural heritage education in schools depends on teacher training. Fifth, the degree to which cultural heritage education is integrated into the curriculum should be strengthened, allowing learners to be educated so that they can cultivate knowledge and critical thinking about cultural heritage, enjoy and protect it, and connect with a sustainable society.

Correlation and Spatial Analysis between the number of Confirmed Cases of the COVID-19 and Traffic Volume based on Taxi Movement Data (택시 이동 데이터 기반 COVID-19 확진자 수와 교통량 간의 상관관계 및 공간분석)

  • Jeon, Seung Bae;Kim, Geon;Jeong, Myeong-hun
    • Journal of the Korean Society of Surveying, Geodesy, Photogrammetry and Cartography
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    • v.39 no.6
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    • pp.609-618
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    • 2021
  • The spread and damage of COVID-19 are putting significant pressure on the world, including Korea. Most countries place restrictions on movement and gathering to minimize contact between citizens and these policies have brought new changes to social patterns. This study generated traffic volume data on the scale of a road network using taxi movement data collected in the early stages of the COVID-19 third pandemic to analyze the impact of COVID-19 on movement patterns. After that, correlation analysis was performed with the data of confirmed cases in Daegu Metropolitan City and Local Moran's I was applied to analyze the effect of spatial characteristics. As a result, in terms of the overall road network, the number of confirmed cases showed a negative correlation with taxi driving and at least -0.615. It was confirmed that citizens' movement anxiety was reflected as the number of confirmed cases increased. The commercial and industrial areas in the center of the city confirmed the cold spot with a negative correlation and low-low local Mona's I. However, the road network around medical institutions such as hospitals and spaces with spatial characteristics such as residential complexes was high-high. In the future, this analysis could be used for preventive measures for policymakers due to COVID-19.

Christian Religious Education's Enchanting Duty : A Curriculum of Hope from the Underside of Civic Polarization, Moral Disimagination, and Learned Helplessness (책임을 노래하는 기독교적 종교교육 : 시민적 양극성, 도덕적 무감각, 학습된 무력감의 저변에서 시작된 희망의 교육과정)

  • Le Tran Mai Anh
    • Journal of Christian Education in Korea
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    • v.77
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    • pp.7-27
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    • 2024
  • This study addresses the crucial role of Christian Religious Education (CRE) amidst civic polarization, moral disimagination, and learned helplessness. It begins her personal background as a 1.5-generation Vietnamese American and her academic engagement in immigrant faith and the challenges of teaching faith in violent contexts. The work underscores the public dimension and impact of religious education, highlighting its potential for fostering critical capacities for public engagement. However, that study observes a prevalent disconnection between congregational culture and the aim of public engagement, leading to a form of learned helplessness among students and communities. The researcher draws on Paulo Freire's concepts of "critical hope" and the need for a curriculum that transcends mere content delivery to foster transformative engagement with societal issues. The document critiques the disimigination machine that undermines critical thinking and collective resistance, as articulated by Henry Giroux, and explores the concepts of "learned helplessness" as a barrier to environmental and social activism. The researcher advocates for a theopoetic and theopolitical approach to education that nurtures hope and practical engagement with the world's injustice. She emphasizes small acts of theopoetic and theopolitical hope as transformative practices, using an example from Ferguson, Missouri, to illustrate how public liturgy and protest can mediate hope and justice. The document concludes with a call for a life-long, life-wide, and life-deep curriculum of enchantment towards responsible participation in societal repair, rooted in Christian hope.

Requirement of Cultural City : Focusing on the Cultural Environmental Policy of Nam-gu, Incheon (문화도시의 충족조건: 인천 남구의 문화환경정책을 중심으로)

  • Kim, Eun-Kyoung;Byun, Byung-Seol
    • Journal of the Economic Geographical Society of Korea
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    • v.9 no.3
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    • pp.441-458
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    • 2006
  • Culture is an indispensable element in modern society in improving the quality of life for city dwellers and stimulating the urbanization. The conspicuous emergence of cultural cities, which is a new paradigm, can be understood in that context. Global cities are pursuing cultural cities, and autonomous regions in Korea are also pushing ahead with the urbanization which combines cultural elements. Specifically, Nam-gu of Incheon has set an example of successful cultural city for other autonomous regions. The cultural environmental policy of Nam-gu is deemed to have retained the infrastructure, cultural urban landscape and living space, and a plenty of contents. The real significance of cultural city lies in the pursuit of sustainable urban development as a culture-friendly city. For that, the direction of cultural environmental city has to be firmly set, and related law and system should be strengthened. Above all, it is critical to pursue human-oriented cultural city by showing citizens what roles they have to play, setting the right direction, and improving the partnership.

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Changes in future precipitation over South Korea using a climate scenario (기후시나리오를 이용한 한국의 미래 강수변화)

  • Lee, Sang-Hun;Bae, Deg-Hyo
    • Proceedings of the Korea Water Resources Association Conference
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    • 2012.05a
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    • pp.199-199
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    • 2012
  • 기후변화로 인한 기상재해의 피해가 전 세계적으로 계속 증가하고 있으며, 특히 기후변화로 인한 집중호우는 시민들의 안전, 재산, 인명피해를 일으키고 있다. 이러한 피해를 최소화하기 위해서는 한 신뢰성 높은 미래 기후시나리오가 필수적이며, 미래 기후시나리오를 바탕으로 하여 기후변화로 인한 향후 발생할 수 있는 위험성의 정도를 전망하여 적응대책을 수립할 필요가 있다. 본 연구에서는 기후시나리오를 이용하여 한국의 미래 강수량변화를 전망한다. 본 연구를 수행하기 위하여, A2시나리오의 ECHO-G/S에서 생산된 기후 시나리오를 이용하여 지역 기후모델인 RegCM3에 적용하여 기후 시나리오를 생산하였다. RegCM3에서 생산된 기후시나리오는 Sub-BATS라는 기법을 이용하여 한반도 5km 해상도의 기후시나리오가 생산되었다. 또한 생산된 기후시나리오와 비교분석을 위하여 전구 20km 해상도의 기후시나리오를 이용하여 미래 강수량 변화를 각 계급별로 분석 하였다. 역학적 상세화 방법에 의해 생산된 기후시나리오는 전체적으로 과소 추정되는 경향이 크게 나타나고 있었으며, 일강수량의 경우 관측자료 보다 상당히 작게 나타나는 특징이 있었다. 역학적 상세화에 의한 강수량은 기존의 연구에서도 비슷한 특징이 나타나고 있었다. 결과적으로 역학적 상세와에 의한 기후시나리오를 이용하여 미래 강수량 변화를 분석하는 것은 강수량의 경향성을 분석할 수는 있지만 정량적으로 분석하는 것에는 한계가 있다. 전구 20km 해상도의 기후시나리오는 전체적으로 관측자료와 상당히 유사하게 모의되고 있었으며, 일강수량 또한 상당히 유사하게 나타나고 있었다. 전구 20km 해상도의 기후시나리오를 이용하여 미래 강수량을 분석한 결과, 전반적으로 증가하는 경향이 있었으며, 21세기 후반에는 약 18%의 연강수량 증가가 나타났으며, 그 중에서도 겨울철의 강수량 증가가 38%로 가장 크게 나타났다. 강수일수 변화는 약 5mm이하는 감소하는 경향이 있었으며, 5mm 이상은 증가하는 경향이 나타났다. 그리고 각 계급별 강수량 변화는, 상대적으로 강수량이 적은 10, 30mm/day는 여름철에 비해 겨울철에 강수량 증가가 크게 나타나고 있었으며, 상대적으로 강수량이 큰 50, 80, 100, 130mm/day는 겨울철에 비해 여름철에 강수량 증가가 크게 나타났다. 본 연구에서 나타난 결과는 미래 수자원 영향평가 및 적응대책에 유용하게 쓰일 것이다.

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Public Practice and Christian Education for Covid-Generation: Uncanny and Incarnational Solidarity (코로나세대 공적 기독교교육의 방향성 연구 : 언캐니(Uncanny)와 성육신적 연대)

  • Yunsoo Joo
    • Journal of Christian Education in Korea
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    • v.74
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    • pp.33-55
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    • 2023
  • This study aims to explore the direction of Christian public practice in the post-COVID era, seeking to overcome the uncanny feeling caused by increased division and exclusion during the pandemic period. Firstly, we will investigate the unequal impact of COVID-19 on the labor market and examine ways to achieve economic justice in the post-COVID era. Subsequently, we will deliberate the role of Christianity in establishing publicness in the digital world and virtual spaces. Finally, viewing COVID-19 as a catastrophe caused by an anthropocentric worldview and exploitation driven by greed, we will explore the tasks of Christianity to overcome the crisis of the Anthropocene. Christian public practice should fulfill its mission of care and stewardship not only in social context but also in an ecological dimension. The author proposes "planetary citizenship education" for a harmonious relationship between human species and the Earth they inhabit.