• Korean journal of applied entomology
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• v.35 no.3
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• pp.232-237
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• 1996

Toxicity of 5 acaricides, 4 insecticides. and 6 fungicides to the twospotted spider mite, Tetranychus urticae Koch, and its predator Amblyseius womersleyi Schicha was assessed in a laboratory using a leaf disk bioassay. Dicofol was equally very toxic to aduly females of T. urticae and A. womersleyi, and caused 46% and 40% mortality of eggs of those two species, respectively. Adult females of A. womersleyi dipped in Abamectin solution showed low mortality(16.6%), while all T. urticae females died within 24 hours after dipping. Three ovicidal acaricides (Tetradifon, Clofentezine, and Hexythiazox) showed no mortality of predator eggs, but more than 90% mortality of T. urticae eggs. Four insecticides (Teflubenzuron, Triflumuron, Diflubenzuron, and Imidacloprid) and three fungicides (Propineb, Fenarimol, and Polyoxin-B captan) were non toxic to the A. womersleyi adult females. The other fungicides (Chlorothalonil, Nuarimol.Mancozeb, and Folpet) were a little toxic to adult females of the predator, showing$\leq$23.3% mortality, It may be suggested from these results that 4 insecticides, 4 acaricides, and 6 fungicides described could be incorporated into the integrated pest management system with A. womersleyi in apple orchard.

• Korean Journal of Medicinal Crop Science
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• v.12 no.1
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• pp.24-30
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• 2004

The immune activities of Ganoderma lucidum Mycelium added garlic extracts (GAM), Ganoderma lucidum Mycelium (GM), garlic extracts (GS) and standard $({\beta}-glucan)$ were compared. GAM enhanced the growth of human immune T cell up to $1.25{\sim}1.46$ times, compared to control group. GAM showed relatively lower cytotoxicity in using normal human lung cell, while GAM showed the most potent inhibitory effect on the human lung carcinoma, compared to GM and GS. The selectivity of GAM was also higher than that of GM and GS. GAM increased the secretion of cytokines, IL-6 and TNF- from human B cell as well as the growth of human immune cells. It can imply that GAM has higher immune activity than GM or GS.

• Ecology and Resilient Infrastructure
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• v.2 no.2
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• pp.147-153
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• 2015

Various adverse effects can occur due to direct exposure from toxic substances when toxic materials are used to restore river ecosystems. Thus, this study performed analysis on the development of toxicity in terms of survival and abnormality rates using embryos of Bombina orientalis living in Korea to analyze the toxicity of materials used in the river projects. The results showed that the toxicity in cement (C group) was the strongest whereas the toxicity in plant-based polyurethane (P1 group) was the weakest. Survival rates of B. orientalis embryos were 100%, 94 - 95%, 66 - 89% and 0% in control, P1, polyurethane (P2) and C groups, respectively. Abnormalities of embryos were 10.5%, 5.3 - 10.5%, 26.3 - 27.8% and 35.7% in control, P1, P2 and C groups, respectively. Furthermore, we verified that having a sufficient curing time reduced toxic substances that were extracted. The above result suggest that cement and polyurethane hamper the early development of amphibians. In conclusion, it is highly important to review biological safety with respect to the selection of materials used to restore rivers. This study shows the importance of the selection of eco-friendly materials and processes.

• Proceedings of the Korean Institute of Intelligent Systems Conference
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• 2002.12a
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• pp.478-481
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• 2002

본 논문에서는 생명체의 면역계에서 중요한 역할을 하는 세포독성 T세포의 생성과정의 하나인 긍정선택(positive selection)과 부정 선택(negative selection)을 모델링하여 침입에 의한 데이터 변경과 바이러스에 의한 데이터 감염 등을 탐지할 때 가장 중요한 요소인 변경 검사 알고리즘을 개발하였다. 제안한 알고리즘은 면역세포의 생성시 MHC 인식부를 형성해 주는 긍정 선택을 자기 인식 알고리즘으로 구현하여 컴퓨터에서 자기로 인식해야하는 파일이나 기능에 대해 MHC 인식부를 형성하고, 또한 항원 인식부를 형성하는 부정 선택을 이용해 변형 검지기(anomaly detector)를 구성한다. 따라서 제안한 알고리즘은 실제 면역세포와 마찬가지로 자신과 침입자 모두에 대한 인식기를 가지고 변경을 탐지하게 된다. 시뮬레이션을 통하여 자기파일의 일부가 변경되었을 때와 블록이 변경되었을 때에 대하여 두 가지 방법을 이용한 변경 검사 알고리즘의 특성과 유효성을 밝힌다.

• Korean journal of applied entomology
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• v.18 no.4 s.41
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• pp.149-152
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• 1979

The relative toxicity of some of the insecticides which have been used for the control of paddy rice insect pests in Korea was evaluated in the laboratory with the brown planthopper (BPH) Nilaparvata lugens, and a predaceous paddy spider Pirata subpiraticus. In order of the relative toxicity (LD5O value to spider/LD50 value to BPH) were PAP (0.4), MPP(0.1), MEP(1.8), diazinon(2.8), carbofuran(7.5), NAC(11.3), BPMC(17.5), Pyridaphenthion(35.9) and MIPC(65.7). MIPC and Pyridaphenthion were considered as having the desirable selective toxicity for the spider and the BPH.

• Korean journal of applied entomology
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• v.34 no.4
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• pp.360-367
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• 1995

The relative toxicity of abamectin was assessed to the predatory mite Amblyseius womersleyi Schicha and to dicofol-resistant and -susceptible twospotted spider mite (TSM) Tetranychus urticae Koch in the laboratory. Abamectin was much les toxic to the predator than to the spider mite. At 0.12 and 0.6 ppm, all TSM adult females of the tow strains were killed within 48 h after dipping n the solutions. The lower concentrations (0.06 and 0.012 ppm) killed more than 77% of TSM female adults of the two strains at 120 h after treatment. However, abmectin did not significantly affect the survival and mobility of A. womersleyi female adults at a concentration of 0.12 ppm but the mortality was slightly increased up to 20~23% at 0.6 and 6 ppm. Abamectin did not significantly affect hatchability of one-day old TSM eggs at 0.06~0.6 ppm. The Four-day old eggs were much more susceptible to abamectin than one-day old eggs were. Within 0.006-6 ppm, abamectin did not affect the hatchability of A. womersleyi eggs and the development of resulting immature predators. When the predator female adults were dipped in 0.6 and 0.12 ppm solution, their reproduction was not affected, but at 6 ppm it was decreased by 35%. However, the reproduction of TSM reduced significantly at concentrations between 0.006 and 0.6 ppm. The differential toxicity of abamectin between TSM and the predator could be of practical importance in managing spider mite populations in the field. Abamectin at selective sublethal concentrations (i.e., 0.012~0.06 ppm) could be of value in adjusting predator/prey ratios in integrated management of spider mites.

• YAKHAK HOEJI
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• v.28 no.1
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• pp.35-48
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• 1984

우리나라 사람들에게는 유달리 높은 간염 발병률이 나타나고 있고 더우기 바이러스성 간염의 경우 풍토병 내지 전염병이라고 할 정도의 양상을 띠고 있다. 현재까지는 이러한 간질환에 적절한 치료약이 없는 점을 감안하여 저자들은 생약으로 부터 간질환에 유효한 성분을 분리하여 치료약으로 사용할 수 있도록 하기위하여 집중적인 연구를 지난 5년간 계속하여 왔다. 연구의 내용은 동서양의 고전및 연구보문을 조사하여 간보호 및 치료학으로 사용한 생약에 관한 문헌적 조사, 이들 생약중 채집 및 구입이 가능한 식물을 구하여 생약엑기스 제조, 이들 엑기스를 간염 동물 모델의 하나인 $CCl_{4}$로 유발시킨 간독성에 대한 보호작용의 검색, 보호작용을 나타내는 생약중에서 자원적 측면을 고려하여 국내에서 많이 생산되는 차전자(Plantago asiatica seeds)를 선택하였고, 차전자로 부터 간보호 작용을 나타내는 유효성분으로 iridoid 계열물질인 aucubin을 분리하였다. 그러나 aucubin이 차전자에 소량 밖에 없으므로 aucubin이 다량 함유된 식나무(Aucuba japonica)로 aucubin 추출자료생약을 바꾼후 aucubin의 간보호 작용을 간염 동물모델인, $CCl_{4}$ D-galactosamine 및 $\alpha$-amanitin등으로 유발시킨 간독성에 대한 보호작용을 보여 주었기 때문에, 이러한 간보호작용의 기전을 규명하는 연구를 진행하였고, 다음은 aucubin 자체의 급성 독성 및 기타 독성 유발여부를 밝히는 연구 등으로 요약 될 수 있다.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.31 no.1 s.49
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• pp.51-58
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• 2005

Alkyl ethoxy sulfate type surfactants, widely used in commercial cleansers, are easily adsorbed to skin to often cause skin irritation and inflammation if not thoroughly rinsed nut. In order to replace or complement existing surfactants, we screened the existing surfactants through protein denaturation method, cell cytotoxicity assay and human IL-1$\alpha$ assay, etc. Fourteen surfactants have been chosen from among too irritant anionic, cationic and/or zwitter-ionic ones and investigated for cell cytotoxicity in human fibroblast cell lines using monolayer culture with the thirteen commercially available cleansers for sensitive skin. From these results, we selected 5 surfactants and 2 commercial cleansers (names not shown), such as sodium laureth sulfate (anionic), sodium cocoyl isethionate (anionic), sodium lauroamphoacetate (zwitter-ionic), and cocamidopropyl betaine (zwitter-ionic), alkyl polyglycoside (non-ionic). 20 formulations were made out of 5 surfactants and five of them were chosen through a protein denaturation method (lower than 3 M sodium dodecyl sulfate solution ($13.2\%$)), cell cytotoxicity and human patch test. These five selected formulations containing preservatives were compared to two selected commercial cleansers by cell cytotoxicity and human IL-1$\alpha$ ELISA assay using dermal equivalent. Finally, we selected the best formulation. To this formulation, fructan ($3\%$ or $5\%$) or/and portulaca extract ($3\%$ or $5\%$) well known for its anti-inflammatory and moisturizing effects were added and investigated for cell cytotoxicity using dermal equivalent. In cytotoxicity assay using dermal equivalent, two formulations containing $5\%$ fructan and $3\%$ or $5\%$ portulaca extract were less toxic than the others. In cytotoxicity assay and human IL-1$\alpha$ ELISA using 3D culture, the selected formulation containing $5\%$ fructan and $5\%$ portulaca extract showed better efficiency than those of the others and 2 commercial cleansers. As a result, we could develop a low irritant and safe liquid cleanser.

• Water for future
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• v.27 no.1
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• pp.69-77
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• 1994

As the standard of living improves, the amount of synthetic detergent consumption greatly increases. Detergents which are not treated in the sewer treatment processes, flow into rivers or waterstreams and accelerate the pollution of the surface water resources. Detergents contain lots of toxicants. And it is difficult to evaluate gross toxicity of each toxicant in thereceiving water. In the study, the acute toxicity of the synthetic detergents for home laundering and kitchen use were monitored with daphnia magna and photobacterium phosphoreum. Seven kinds of detergents were tested to evaluate the acute toxicity. The mean 24hr, 48hr-LC50 of the synthetic detergent for home laundering were 4.25%, 2.50% and these for kitchen use were 2.01%, 1.36% respectively. And the mean 5min, 15min-EC50 of the synthetic detergent for home laundering were 1.83%, 1.02%.

• Journal of Digital Convergence
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• v.17 no.3
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• pp.305-312
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• 2019

This study was conducted to effect the cell activity and cytotoxicity of dentifrice. For the study, 6 kinds of general dentifrice, 3 kind of whitening dentifrice, 2 kinds of natural dentifrice and SLS(sodium lauryl sulfate) of positive control group. Immortalized human gingiva fibroblast cell was used for the study, WST test for cell activity and Agar diffusion test for cytotoxicity. Agar diffusion test showed high cytotoxicity in general dentifrice test group and whitening dentifrice test group, but low cytotoxicity in natural dentifrice test group. As a result of cell nucleus staining, cell shape and nuclear activity showed that the highest activity in natural dentifrice group, followed by whitening dentifrice group and general dentifrice group. As a result of this study, the cytotoxicity of different ingredient and according to the use to dentifrice. As a result of this study, we confirm cytotoxicity of kind and components according to the purpose of using dentifrice. Therefore, it is necessary to indicate the detailed ingredients of dentifrice for the smart choice of consumers.