• Title/Summary/Keyword: 선충분류동정

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Isolation and Identifieation of Entomopathogenic Nematodes from Soil and Insect (토양과 곤충 사체로부터 곤충병원성 선충의 분리 및 동정)

  • 한상미;한명세
    • Korean Journal of Environmental Biology
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    • v.17 no.3
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    • pp.321-330
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    • 1999
  • Nematodes were isolated using silkwom trap through the investigation of 100 soil samples from various biotopes in Korea. The 30 nematode strains from soil and dead insects by the pathogenicity aganinst silkworms (Bombyx mori mori) and insect pests of Calliphora vomitoria, Pseufazetia separata, Palomena angulosa, and Melolontha incana. Mortailty of the silkworm larvae and pupae were as high as 100% by nematode infection, those of insect of pests were varied from 20 to 100%. The 30 strains of entemopathogenic nematodes were classified into five groups of Rhabditidae, Diplogatroidae, Heterorhabitidae, Steinernematidae, and Tylenchida by morphological criteria. The genetic relationships among the 30 nematode strains were analyzed by various RAPD bands with twenty primers. The 30 nematode strains were classified into six major subgroups on the basis of the genetic similarity coefficient of 0.853. The grouping by RAPD was agree with those of morphological taxa in discrimination of the higher group, however, was not completely agree in the subgroup. The family Steinernematidae belong to Rhabditida was clarified as closer to the Tylenchida, rather than the other Rhabditida of Heterorhabitidae, Rhabditidae, and Diplogatroidae in genetic distance valule. From the result of the morphological classification and RAPD of the genomic DNA showed that genetic relationship analysis furnish infurmation on phylogenetic classification and relationships of entomopathogenic nematodes. The application of genetic similarity will overcome the limitation of taxonomy and classification of morphologically simple nematode. Several primers were confirmed those utility of identification for individual nematode strains, the methods of molecular genetics secured the simplicity, rapidity and accuracy on the selection of entomopathogenic nematodes.

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A Taxonmical Study on The Family of Longidoridae(Nematoda) in Korea. 1. There Unrecorded Species of Longidoridae (한국에 있어서 Longidoridae과 선충의 분류학적 연구 1.한국산 Longidoridae과의 3 미기록종에 관하여)

  • 최영연;문일성
    • Korean journal of applied entomology
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    • v.27 no.3
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    • pp.165-170
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    • 1988
  • Taxonomical study on the Longidoridae from korea were undertaken at Kyungsangbuk-do. Three species belomging to two Longidoridae were identified. Xiphinema zulu, X. setatiae and Longidoridae sylphus were newly reported from Korea.

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Isolation and Identification of Micromonospora sp. Showing Nematocidal Activity Against Pine Wood Nematode (소나무재선충에 대한 살선충 활성을 나타내는 Micromonospora sp.의 분리 및 동정)

  • Park, Dong-Jin;Lee, Jae-Chan;Kim, Pan-Kyung;Kim, Chang-Jin
    • The Korean Journal of Pesticide Science
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    • v.12 no.1
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    • pp.97-101
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    • 2008
  • For the isolation of Actinomycetes showing nematocidal activity against Pine wood nematode, Bursaphelenchus xylophilus, about 2000 culture broth of Actinomycetes were tested and their activity were compared with that of Streptomyces avermitilis resulting a selected strain AW050027. The cultural, morphological and physiological analysis was performed for the identification of a selected strain. Phylogenetic analyses based on 165 rDNA gene sequences showed that the selected strain AW050027 belonged to the genus Micromonospora and M. corioriae $NAR01^T$ was the closest neighbors, sharing 98.9% 165 rDNA gene sequence similarity.

Distribution of Plant-parasitic Nematodes in Fruit Vegetable Production Areas in Korea and Identification of Root-knot Nematodes by Enzyme Phenotypes (과채류 시설재배지 식물기생선충 분포 및 효소표현형을 이용한 뿌리혹선충의 동정)

  • 조명래;이봉춘;김동순;전흥용;임명순;이정운
    • Korean journal of applied entomology
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    • v.39 no.2
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    • pp.123-129
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    • 2000
  • This study was conducted to analyse the distribution of root-knot nematodes (Meloidogyne spp.) in fruit vegetable production areas in Korea. Soil samples were collected from greenhouses in Sungju (Kyungpook), Yeoju (Kyungki), Haman (Kyungnam), and Chungwon (Choongpook) provinces in 1997-1999. Plant parasitic nematodes were separated for density counting and some of the root-knot nematodes were identified using enzyme phenotypes of malate dehydrogenase (MDH) and esterase (EST). Among the 185 farms in Sungju province, Meloiciogyne spp. were detected from 99 farms (53.5%). Other plant parasitic nematodes detected were; Helic~otylmchuss pp. from 7 farms, Aphelenchus spp. from 43 farms, and Criconematids from 26 farms. Using the female enzyme phenotypes of MDH and EST. the four major root-knot nematodes in Korea, M. incognitc~(M I), M. uretznri~(M~ A), M. huplu (MH), and M. juvunica, could be identified. In the enzyme phenotype identification of 13 populations collected from Sunnam in Sungju province, 6 populations were identified as MA, 5 populations were identified as MI, and 2 populations were mixed with MI and MA. Among the 6 populations from Chojun in Sungju province. 4 populations were MA, one population was MI, and one population showed enzyme phenotypes of unknown species. Among the 14 populations of Yeoju province, l I populations were MH and 3 populations were MA.

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Cryphodera coxi(Wouts, 1973) Luc, Taylor and Cadet, 1978(Tylenchida:Heteroderidae) First Reported from Korea (사과둥근선충, Cryphodera coxi(Wouts, 1973) Luc, Taylor and Cadet, 1978(참선충목:씨스트선충과) 한국말기록속 1종)

  • 최영연;한상찬;김병회
    • The Korean Journal of Soil Zoology
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    • v.5 no.1
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    • pp.33-37
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    • 2000
  • During the survey on apple parasitic nematodes from Korea, Cryphodera coxi(Wouts, 1973) Luc, Taylor and Cadet, 1978 was discovered for the first time from Korea. The specimens preserved in the Nematology Laboratory, School of Bioresource Sciences, College of Natural Sciences, Andong National University.

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Morphological and Phylogenetic Characteristics of Nematophagous Fungi (식물기생성 선충 포식곰팡이의 형태 및 계통분류학적 특성)

  • Kang, Doo-Sun;Jeon, Han-Ki;Son, Hee-Seong;Whang, Kyung-Sook;Cho, Cheon-Whi
    • Applied Biological Chemistry
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    • v.50 no.2
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    • pp.101-106
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    • 2007
  • Twenty-two strains of nematophagous fungi were isolated from 100 soil samples. Nematophagous fungi were classified into three categories; 3-dimensional adhesive nets (A group), 2-dimensional adhesive nets (B group) and constricting ring (C group). Nine strains were selected and identified on the basis of morphological characteristics (hypha, conidiophore, form and size of conidia, number of conidia, node of conidophore, number and location of septa, size and color of chlamydospore) and ITS (internal transcribed spacer) region of rDNA sequences. As the results, the isolated were identified as belonging to the species of Monacrosporium thaumasium (Kan-2, Kan-4, Kan-11), Arthrobotrys oligospora (Kan-9, Kan-13, Kan-20, Kan-21), A. musiformis (Kan-12), and A. dactyloides (Kan-22).

Identification of Root-lesion Nematode (Pratylenchidae: Pratylenchus ) Intercepted on Imported Plants (수입식물에서 검출된 뿌리썩이선충(뿌리썩이선충과: 뿌리썩이선충속)의 진단)

  • Kim, Dongwoo;Chun, Jae-Yong
    • Korean journal of applied entomology
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    • v.53 no.1
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    • pp.51-57
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    • 2014
  • We identified the five root-lesion nematode species, Pratylenchus crenatus, P. fallax, P. kumamotoensis, P. panamaensis and P. penetrans from intercepted in quarantine inspection over the past five years. Their diagnostic characters are including number of lip annuli, stylet length, shape of the labial region, presence or absence of males, structure of lateral fields, shape of spermatheca, length of the post-vulval uterine sac and shape of tail and so forth. We described the photos, measurements and morphological characters.

New Record of Pratylenchoides utahensis Baldwin, Luc & Bell (Tylenchida: Pratylenchidae) from Korea (한국미기록 엉겅퀴뿌리썩이선충, Pratylenchoides utahensis Baldwin, Luc & Bell(찬선충목: 뿌리썩이선충과))

  • Young-Eoun Choi;Dong-Ro Choi;Byeong-Young Park
    • Korean journal of applied entomology
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    • v.39 no.1
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    • pp.1-4
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    • 2000
  • Pratylenchoides utahensis Baldwin, Luc & Bell, 1983 is recorded for the first time from Korea. Morphological characteristics are described based on the specimens preserved in Nematology Laboratory, Entomology Division, National Institute of Agricultural Science and Technology; and Department of Agricultural Biology, Kyungpook National University.

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