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Solvent-tolerant Lipases and Their Potential Uses (유기용매 내성 리파아제와 그 이용가능성)

  • Joo, Woo Hong
    • Journal of Life Science
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    • v.27 no.11
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    • pp.1381-1392
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    • 2017
  • This review described solvent-tolerant lipases and their potential industrial, biotechnological and environmental impacts. Although organic solvent-tolerant lipase was first reported in organic solvent-tolerant bacterium, many organic solvent-tolerant lipases are in not only solvent-tolerant bacteria but also solvent-intolerant bacterial and fungal strains, such as the well-known Bacillus, Pseudomonas, Streptomyces and Aspergillus strains. As these lipases are not easily inactivated in organic solvents, there is no need to immobilize them in order to prevent an enzyme inactivation by solvents. Therefore, the solvent-tolerant lipases have the potential to be used in many biotechnological and biotransformation processes. With the solvent-tolerant lipases, a large number insoluble substrates become soluble, various chemical reactions that are initially impossible in water systems become practical, synthesis reactions (instead of hydrolysis) are possible, side reactions caused by water are suppressed, and the possibility of chemoselective, regioselective and enantioselective transformations in solvent and non-aqueous systems is increased. Furthermore, the recovery and reuse of enzymes is possible without immobilization, and the stabilities of the lipases improve in solvent and non-aqueous systems. Therefore, lipases with organic-solvent tolerances have attracted much attention in regards to applying them as biocatalysts to biotransformation processes using solvent and non-aqueous systems.

A Critical Analysis on Social Welfare Researches in Korea (우리나라 사회복지학 연구경향에 관한 연구 - <한국사회복지학>에 실린 경험분석연구를 중심으로 -)

  • Kim, Yoon-Ock
    • Korean Journal of Social Welfare
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    • v.35
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    • pp.85-105
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    • 1998
  • This article examined the trend of 125 empirical researches which were published in Jr. of Korean Social Welfare from the first issue to no. 33. in terms of theoretical and methodological orientations. The content analysis was employed for the purpose of the study. Since 1979, the number of empirical researches was in the trend of increasing. The findings from this research were as follows. 1) Among 166 authors, 96.4% were majored in social welfare. Also 6.0% were practitioners and the rest of them were in the position of professors or researchers. The outcome of lack of interdisciplinary co-work and researcher-practitioner co-work led the article to conclude that the nature of applied social science of social welfare was not so actively pursued in Korea. 2) It was almost impossible to find researches which studied same theme or employed same analytical framework. This meant that the work of re-verifying and proving the contray could not be done although it was essential for theory-building. In other words, the disciplinary of social welfare was far behind in the process of theory-building. 3) The methodology upon which most of researches were relied was quantitative methodology(92.8%). The article concluded 'paradigm shift' was not begun in the disciplinary of social welfare yet. 4) The study concluded that the particularity of empirical researches of social welfare in Korea was descriptive-configurative study. Whereas 65.5% of 125 empirical studies were descriptive-configurative, 25% were hypothesis - model test and only 6% causal analysis. 5) The most applied statistic models through the period from 1979 to 1997 were descriptive statistics such as frequency, chi square test, Pearson's r. More advanced statistics such as logit regression, probit regression, path analysis, covariance structure analysis were shown since 1990.

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A Study of High School Students' Conceptions of Mixing Phenomena Related to Dissolution and Diffusion (용해.확산과 관련된 혼합현상에 대한 고등학생들의 개념 유형 분석)

  • Hur, Mi-Youn;Jeon, Hey-Sook;Paik, Seoung-Hey
    • Journal of the Korean Chemical Society
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    • v.52 no.1
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    • pp.73-83
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    • 2008
  • The purpose of this study was to investigate the types of conceptions of mixing phenomena related to dissolution and diffusion in high school students. The subjects of the investigation consisted of 108 students who took chemistry I course at 11th grade and 29 students who took chemistry II course at 12th grade. For this study, it was found that the many students had the alternative conception that chalk didn't dissolve in water because chalk was a nonpolar material. Most of the students understood the phenomena which carbon tetrachloride and water will not mix as the attraction conception. But many of the other students understood the phenomenon as characteristic of the materials such as difference of density. Many of the students understood the phenomenon of mixing ethanol and water constantly as ‘Attraction conception'. The phenomenon which is mixed ink and water was just accepted by the most students as the spreading of ink in water without understanding the reason of mixing. The phenomena of mixing iodine and carbon tetrachloride was understood as ‘Space conception' or ‘Attraction conception'. It could be inferred that the diverse alternative conceptions related to dissolution and diffusion phenomena were generated by the absence of entropy concept. Therefore, the explanations of science textbooks related to dissolution and diffusion phenomena need to change for students to understand them correctly.

A Study on Fire Facilities of Urbane Buildings (도시건축물의 소방시설에 관한 연구(I))

  • 김소수
    • Fire Science and Engineering
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    • v.6 no.2
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    • pp.15-24
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    • 1992
  • 우리나라는 1960년대의 경제개발계획에 따른 공업화 시축의 추진으로 산업의 발달과 경제성장은 인구를 도시로 집중시키면서 도시건축물은 대형화, 고층화, 과밀화 현상을 가져왔다. 한편, 국민생활의 향상과 산업활동의 다양화로 화재발생의 주요원인이 되는 전기, 가스, 유류등의 사용량이 해마다 늘어나고 있어 화재의 위험을 가중시키고 있다. 최근 10연간('81~'90) 전국에서 발생한 화재사고는 9만 5,154건이 발생하여 인명피해 1만1,117명, 재산피해 1,859억 2,200만원의 많은 손실을 가져 왔으며, 이 중에는 서울에서 발생한 화재가 3만6,089건으로 전체의 37.9%을 차지하고 있다. 화재가 발생할 경우 대형 참사를 방지하고 귀중한 인명피해는 물론 경제적 손실을 최저로 줄일 수 있는 소방시설을 도시건축물에 설치하여 이에 대비하는 것이 무엇보다 귀중한 일이다. 이는 화재예방 또는 발생 초기에 이를 감지, 통보하고 피난하며 소화활동에 이르는 모든 방재 및 소화를 위한 소방시설의 유용성을 최대로 발휘할 수 있기 때문이다. 본 논문에서는 서울특별시를 중심으로 도시건축물에 설치된 소방시설의 유지 관리 실태와 화재시 소방시설의 이용실태를 분석하여 문제점을 도출하고 이에 대한 개선방향을 제시하므로서 예방소방행정의 발전을 기하고저 하였으며, 그 내용을 요약하면 다음과 같다. 제1장에서는 서론부분으로서 연구의 목적과 범위및 접근 방법을 서술하였고, 제2장에서는 도시소방행정의 특성 및 소방환경의 변화로 먼저 소방행정의 의의와 도시소방행정의 유형으로 예방소방과 진압소방 그리고 구급.구조업무등을 살피고. 도시소방환경변화에 따른 화재발생추세를 살펴 보았다. 제3장에서는 도시건축물에 설치하는 소방시설의 설치.유지 및 이용실태를 검사.분석하였다. '90년도 서울시내 소방서에서 실시한 소방대상물에 대한 소방검사와 방화관리자에 대한 설문조사를 실시하여 소방시설의 유지관리실태를 통계.분석한 결과, 소방시설이 양호한 소방대상물은 전체의 75.9%이며, 불량소방대상물은 24.1%로 나타났다. 그리고 화재가 발생한 소방검사대상물에 대한 화재현장 조사결과 화재시 소방시설을 사용한 소방대상물은 전체의 72.1%를 차지하고 있다. 제4장에서 는 소방시설의 문제점으로 \circled1 소방설비부실공사, \circled2 소방시설 유지관리능력부족, \circled3 소방검사제도 불합리등이며, 이에 대한 개선방향으로 \circled1 소방설비 시공자의 지도. 감독강화, \circled2 자체시설관리능력향상, \circled3 예방소방행정제도의 개선을 제시하였다. 제5장은 결론으로, 우리나라가 최근 경제성장과 산업의 발달로 도시건축물의 화재발생 위험이 가중되면서 이에 대한 소방안전대비책이 요구된다. 이에는 도시건축물에 방화시설과 소방시설을 완비하고, 그리고 자격있고 유능한 방화관리자 선임하여 자체 소방계획을 수립하여 시설점검, 정비와 유지관리를 철저히 한다면 어떠한 화재도 예방 또는 초기에 진압할 수 있고, 또한 입주검자에 대한 소방교육 및 훈련을 지속적으로 수행하여 나간다면 여하한 도시건축물의 소방안전도 그 목적을 달성하리라고 생각한다.

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Solid-Phase Refolding Technology in Recombinant Proteins Recovery: Application Examples to Various Biopharmaceutical Proteins (유전자재조합 단백질 회수 공정에서의 고체상 재접힘 기술: 여러 바이오의약 단백질에의 적용 사례)

  • Kim, Min Young;Suh, Chang Woo;Kim, Chang Sung;Jo, Tae Hoon;Park, Sang Joong;Choi, Won Chan;Lee, Eun Kyu
    • Korean Chemical Engineering Research
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    • v.43 no.2
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    • pp.187-201
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    • 2005
  • Bioprocessing technologies utilizing 'biorecognition' between a solid matrix and a protein is being widely experimented as a means to replacing the conventional, solution-based technology. Frequently the matrices are chromatographic resins with specific functional groups exposed outside. Since the reactions of and interactions with the proteins occur as they are attached to the solid matrix, this 'solid-phase' processing has distinct advantages over the solution-phase technology. Solid-phase refolding of inclusion body proteins uses ion exchange resins to adsorb denaturant-dissolved inclusion body. As the denaturant is slowly removed from the micromoiety around the protein, it is refolded into a native, three-dimensional structure. Once the refolding is complete, the folded protein can be eluted by a conventional elution technique such as the salt-gradient. This concept was successfully extended to 'EBA (expanded bed adsorption)-mediated refolding,' in which the denaturant-dissolved inclusion body in whole cell homogenate is adsorbed to a Streamline resin while cell debris and other impurity proteins are removed by the EBA action. The adsorbed protein follows the same refolding steps. This solid-phase refolding process shows the potential to improve the refolding yield, reduce the number of processing steps and the processing volume and time, and thus improve the overall process economics significantly. In this paper, the experimental results of the solid-phase refolding technology applied to several biopharmaceutical proteins of various types are presented.

Making a Civil War Surrounding History in Cyber Space Focused on 5·18 Discourses in Ilbe Storehouse (사이버 공간에서의 역사의 내전(內戰)화 '일간베스트저장소'의 5·18 언설을 중심으로)

  • Jung, Soo-Young;Lee, Youngjoo
    • Korean journal of communication and information
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    • v.71
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    • pp.116-154
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    • 2015
  • Officially given a historical signifier as Gwangju Democratic Movement since 1987, far-right and conservative group have restated that $5{\cdot}18$ is a rebellion and a riot that was committed by rebellious elements who obeyed North Korea's command or who were connected with North Korea. As those who had been responsible for the rebellion, revolt and riot were rewarded, far-right and conservatives' collective narrative that a country was born where the pro-North Korea left became dominated aroused extreme hostility towards $5{\cdot}18$. Far-right and conservatives involved in many different fields such as political party, university, press and media and civil group carry out incendiary discourse politics with intention to reestablish history and memory of $5{\cdot}18$ in their own story. Many people at online sites such as Ilbe Storehouse who are considered 'young right wing' is a main route to spread the far-right groups' remarks on $5{\cdot}18$. Ilbe is a main channel to reconstitute and reproduce the far-right conservatives' remarks and information on $5{\cdot}18$. Ilbe is one of main area where remarks of disparagement and ridicule, hostility and hatred on $5{\cdot}18$ unfurl. This study collects $5{\cdot}18$-related remarks and stories unfolded at Ilbe and examines how these remarks and stories make significance as to $5{\cdot}18$ and how information resources which remarks are dependent upon are connected each other. In this process, this study intends to find implications of incendiary politics that echoed of remarks on $5{\cdot}18$ have which at the online site Ilbe and by the far-right conservatives.

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Pedagogical Content Knowledge of Socio-Scientific Issues: Characterizing Teachers' Experiences (과학 기술 관련 사회쟁점 교육에 대한 과학 교사들의 SSI-PCK 사례연구)

  • Chung, Haengnam;Ryu, Suna
    • Journal of The Korean Association For Science Education
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    • v.37 no.4
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    • pp.679-691
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    • 2017
  • Despite the growing importance of socio-scientific issues (SSI), little work has focused on teachers' pedagogical knowledge, and few studies have examined how in-service teachers use and apply SSI-related instructional methods and strategies. Consequently, the purpose of this study was to explore how science teachers with lots of SSI teaching experience determined teaching orientation, selected and revised instructional methods, and devised assessments in science classroom. We used a case study approach aimed at a deep description of these teachers' teaching experiences and employed semi-structured and in-depth interviews with five experienced teachers. Our findings indicate that teaching orientation and learning objectives seemed to influence the selection and modification of instructional strategies and methods. In addition, students' learning experiences or classroom environment were considered and modified in light of achieving these learning objectives. However, introducing SSI into the classroom assessment is not seriously considered by most teachers. This study can provide teachers with useful information when designing and developing SSI classes, taking into account various aspects of the PCK such as learning orientation, instructional methods, learner expereince and curriculum standards.

Filter Media Specifications for Low Impact Development: A Review of Current Guidelines and Applications (LID 시설 여재에 관한 기술지침 및 적용에 관한 고찰)

  • Guerra, Heidi B.;Kim, Lee-Hyung;Kim, Youngchul
    • Journal of Wetlands Research
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    • v.21 no.4
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    • pp.321-333
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    • 2019
  • A primary aspect of low impact development (LID) design that affects performance efficiency, maintenance frequency, and lifespan of the facility is the type of filter media as well as the arrangement or media profile. Several LID guidelines providing media specifications are currently available and numerous studies have been published presenting the effectiveness of these systems. While some results are similar and consistent, some of them still varies and only a few focuses on the effect of filter media type and arrangement on system performance. This creates a certain level of uncertainty when it comes to filter media selection and design. In this review, a synthesis of filter media specifications from several LID design guidelines are presented and relevant results from different laboratory and field studies are highlighted. The LID systems are first classified as infiltration or non-infiltration structures, and vegetated or non-vegetated structures. Typical profiles of the media according to classification are shown including the different layers, materials, and depth. In addition, results from previous studies regarding the effect of filter media characteristics on hydraulic and hydrologic functions as well as pollutant removal are compared. Other considerations such as organic media leaching, clogging, media washing, and handling during construction were also briefly discussed. This review aims to provide a general guideline that can contribute to proper media selection and design for structural LIDs. In addition, it also identifies opportunities for future research.

Ji-Won Park's Geographical View on Qing Dynasty in the Late 18th Century(I) ('열하일기(熱河日記)'에 비친 연암 박지원의 지리관 일 고찰(I) -자연지리적 인식.문화지리적 인식.지역지리적 인식을 중심으로-)

  • Sohn, Yong-Taek
    • Journal of the Korean association of regional geographers
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    • v.10 no.3
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    • pp.497-510
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    • 2004
  • This study searched for comparison with geography of Qing China, origin of geographical name, people's feature and costume, description on regional geography, and perception on locality on view of a geographer, Ji-Won Park, a renowned Korean scholar of practical learning on the late 18th century. This study on the first three chapters of his Yorha Ilgi - "Crossing the Amnok(Yalu) River("渡河錄")," "Several Records on Senyang("監京雜識")," and "Writing Essays, Riding Horses("馹迅隨筆")," focusing on landscape of Qing China, inquiry on geographical name, feature and costume, and descriptions on locality, gives a glimpse on Park's geographical view on geography of Qing China. His writing style to compare landscapes between Korea and Qing China, full of his love for Korean landscape, is very delicate and clear, Although he sentimentalized when he passed through Bonghwang-castle(鳳凰城) in China, his subtle and beautiful writing explains that land and mountains in Han-yang(Seoul) are much more wonderful than those in China. His vivid depiction of landscape and weather on his way in Qing China is remarkable and explanation on geographical names like Parhae(渤海), Yoha(遼河), Taejaha(太子河), Senyang(瀋陽) etc. is enough to stimulate readers' curiosity on geography.

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A Study for Safety Management on the Basis of Lateral Displacement Rates of Anchored In-situ Walls by Collapse Case Histories (붕괴 사례를 통한 앵커지지 가설흙막이벽체의 수평변위속도에 의한 안전관리 연구)

  • Chung, Dae-Seouk;Lee, Yong-Beom
    • Journal of the Society of Disaster Information
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    • v.14 no.3
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    • pp.367-378
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    • 2018
  • Purpose: The objective of this study is to present a reasonable safety management of the anchored in-situ wall systems constructed in the ground conditions consisting of multi-layered soils underlain by bedrocks in the urban area of Korea. Method: Field measurements collected from collapse case histories with deep excavations were analyzed for the safety management of the wall systems supported by the earth anchors in terms of lateral displacement rates. Results: The average maximum lateral displacement rate in a collapsed zone of the in-situ wall significantly increased upon the completion of the excavation. Particularly, the collapse of the in-situ wall system due to the sliding occurring along the discontinuities of the rock produced a considerably large lateral displacement rate over a relatively short period. Conclusion: For predicting and preventing the collapse of the wall system during or after the excavation work, the utilization of the safety management criteria of the in-situ wall system by the lateral displacement rate was found to be much more reasonable in judging the safety of earthworks than the application of the quantitative management criteria which have been commonly used in the excavation sites.