• Proceedings of the Korean Statistical Society Conference
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• 2004.11a
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• pp.111-116
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• 2004

스트레스 수준이 논을 것으로 예상되는 공군사관학교 수험생의 신체검사 결과로부터 신체건강 지표인 혈액 내 생화학적 요인들과 정신건강 지표인 다면 인성검사 점수를 추출하여 통계적 상관성 및 유의성을 비교 분석하였다 사관생도들 중 $10\%$의 표본을 추출하여 사관생도와 수험생 집단간의 특성 비교를 위한 비교집단으로 이용하였다. 수험생과 사관생도 집단 간의 특성 요인 비교는 Mann-whitney U 검정과 Kruskal-Wallis 검정을 이용하였다. 수험생 집단 내에서 혈중 요인 및 정신건강 지표인 MMPI 점수는 정상범주에 있었고 두 요인 간 상관성은 발견할 수 없었다. 그러나 사관생도와 수험생 집단 간의 특성 요인들에 대한 유의성 검정 결과, 특히 2학년생도들의 혈액 내 혈구 수 및 활동성 또는 적극성과 관련된 MMPI 하위척도에서 수험생 집단과 매운 유의한 차이를 보이는 특이한 현상을 발견할 수 있었고 이에 대한 해석을 언급하였다.

• Survey Research
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• v.7 no.1
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• pp.29-53
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• 2006

This paper analyzes which of the military sub-fields has an effect on a soldier's evaluation of South and North Korean military power. There are three objectives of this paper. First, it is to analyze whether the South is superior to the North in the overall military power and military sub -fields, without the presence of the US armed forces in South Korea. Second, it is to assess which of the military sub-fields influences the overall evaluation of military power through a regression analysis. Finally, it is to look at whether the professional soldiers have a motivational bias, due to an argument that the military personnel tend to exaggerate the inferiority of their own military power to get more budget for defense construction. Research in 2002 and 2003 tell us that South Korean military power is slightly inferior to North Korea's. However, research in 2005 shows that military power of South and North Korea is almost equal. The sub-fields in which South Korea is superior are in the order of War Sustainability, Information Warfare, Air Force, and Navy. North Korea is regarded to be superior in the fields of Atomic Biological Chemical Warfare Capability, Special Force, Reserve Mobilization, and Army. The higher ranked soldiers tend to evaluate that the North is superior to the South.

• Journal of Applied Biological Chemistry
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• v.52 no.3
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• pp.109-115
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• 2009

The gene encoding a putative aspartate aminotransferase in Xanthomonas oryzae pv. oryzae (Xoo) was cloned using PCR technique. The gene was ligated with pET-21(a) vector containing His6 tag and expressed in E. coli BL21(DE3). Affinity purification of the recombinant aspartate aminotransferase with Ni-NTA resin resulted in one band by SDS-PAGE analysis. The purified enzyme showed a molecular weight of 43 kDa, as expected. The enzyme was the most active toward L-aspartate as an amino donor, indicating that the purified enzyme is one of aspartate aminotrans-ferases exist in Xoo. Optimal activity of the enzyme was observed at around pH 7.5 and stability was much higher at alkaline pH rather than acidic pH values. The enzyme was considerably activated by the presence of manganese ion, showing about 157% of control activity at 1.0 mM.

• Journal of Applied Biological Chemistry
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• v.53 no.3
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• pp.132-138
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• 2010

A gene encoding a putative alanine racemase in B. pseudomycoides was cloned and expressed in Escherichia coli BL21(DE3) using a pET-21 vector harbouring 6xHistidine tag. Affinity purification of the recombinant alanine racemase with a nickel resin resulted in one band by SDS-PAGE analysis. The purified enzyme showed a molecular weight of 46 kDa. The enzyme was the most active toward L-alanine and secondly D-alanine, implying that the enzyme is an alanine racemase. D-cysteine significantly inhibited the enzyme activity and also L-cysteine to a lesser extent. The enzyme was considerably activated by addition of pyridoxal-5'-phosphate (PLP), showing that 73% increase in activity was observed at 0.3 mM, compared to control. The enzyme was the most active at pH 9.0 and more stable at alkaline pHs than acidic pHs.

• The Korean Journal of Pesticide Science
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• v.12 no.4
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• pp.335-341
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• 2008

In this study, hexaconazole 5% SC, an ergosterol biosynthesis inhibitor, was tested on groundnut with its recommended ($500\;mL\;ha^{-1}$) and higher ($2,000\;mL\;ha^{-1}$) concentrations under greenhouse conditions in India. Its influence on biochemical constituents of groundnut plants was assessed apart from its disease management potential against late leaf spot caused by Phaeoisariopsis personata (Berk and Curt). Likewise, leaf samples were collected from hexaconazole 5% SC-sprayed plants at different time intervals. Thereafter, their analyses showed considerable differences in the plant constituents, such as chlorophyll, soluble protein, and total phenol contents and the activity of nitrate reductase enzyme. The induction activity of defense-related enzyme, peroxidase, was also analyzed. However, no difference was observed in the isozymic pattern. Moreover, the ground kernels collected from treated plants also showed no difference in the estimated carbohydrate and other constituents.

• Research in Plant Disease
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• v.13 no.1
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• pp.24-29
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• 2007

A total of 35 strains of Ralstonia solanacearum isolated from wilted pepper and tomato plants in Korea were analyzed for their genetic diversity by bacteriological, pathological and molecular biological approaches. All the strains were identified as R. solanacearum biovar 4 on the basis of physiological and biochemical tests, and species-specific PCR primers. Pathogenicity of the strains was confirmed by inoculating on 4-week-old pepper and tomato seedlings. Using cluster analysis based on repetitive sequence-based polymerase chain reaction (rep-PCR) genomic fingerprints, R. solanacearum strains isolated from pepper and tomato in Korea divided into 6 groups showing a high degree of genetic diversity at 55% similarity level. The genetic diversify of strains was not significantly correlated with their geographic origins and host plants.

• 한국생물공학회:학술대회논문집
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• 2000.04a
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• pp.512-515
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• 2000

In this study, ${\delta}-aminolevulinic$ acid, porphobilinogen, glycine and levulinic acid were succesfully separated by capillary electrophoresis(CE). We established the separative conditions of the mixture containing four components by CE. The borate buffered solution was used for CE electrolyte, and its pH was adjusted to $9.25{\sim}9.42$. Under constant current or constant voltage, higher concentraion of borate produced better resolution of four components, but adversely affected migration rates , resulting in longer analysis time. While migration time was faster with increase in applied voltage, but adversely affected resolution. Each component was separated well in borate buffer of 30mM at the applied voltage of 20kV.

• Proceedings of the Korea Technology Innovation Society Conference
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• 2017.05a
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• pp.501-535
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• 2017

이 연구는 한국 학계가 그간 많은 성장을 일구어 왔음에도 질적 수준에 대해서 지속적인 비판을 받고 있는 현 상황에 의문을 갖고 연구를 시작하였다. 최근 연구의 질적 수준 제고를 위해 연구개발 성과평가 제도가 전면적인 변화를 겪고 있음에도 정작 한국 학술연구의 질적 수준 문제가 어떠한 양상으로 나타나고 있는지 과학적인 증거를 제시하고자 하는 노력은 지금까지 매우 미흡했다. 이에 이 연구에서는 한국 연구의 양적 성장과 질적 성장 간의 괴리가 어떠한 양상으로 나타나는지 가시적인 일면을 제시하기 위하여 한국 연구자가 포함된 모든 SCI 논문을 수집하고 이를 대학, 출연연, 기업, 해외기관, 기타 소속 저자로 구분하여 양적 추세와 피인용횟수 추세를 비교하고자 하였다. 분석결과 해외기관 소속 저자와 국내기관 소속 저자 간에 유의미한 수준의 피인용횟수 격차가 나타나고 있으며, 이러한 격차는 시간의 흐름에 따라 해소되지 않고 있는 것을 확인하였다. 또한 분야별로 격차가 다르게 나타나 생화학 분자생물학이나 신경과학 신경학 등의 분야에서 피인용횟수의 격차가 보다 높게 나타나고 있었다. 이러한 결과는 최근 한국 공공연구 영역에서 전면적으로 일어나고 있는 질적 평가체제로의 전환 움직임에 대해서 그 세부적인 설계와 차등 요건의 적절성에 대해 다시 한 번 생각해볼 수 있는 계기를 제공한다.

• Proceedings of the KSMRM Conference
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• 1999.04a
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• pp.1-9
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• 1999

핵자기 공명(NMR:nuclear magnetic resonance)은 19451건 Bloch, Procell등에 의해 처음으로 실험적으로 입증된 후에 생화학 및 약학 분야에서 분자단위의 물질의 성분, 구조, 대사물질의 역학적 운동 및 상태에 관한 정보를 아는데 유용한 기법으로 사용 되어 왔다. 의학분야에서는 Lauterber등에 의해 자기공명영상볍(Magnetic Resonance Imaging:MRI)으로 개발되어 질병의 진단 및 치료에 많은 공헌을 하게되었다. 해부학적영상 뿐 아니라 분석방법으로 병변 부위 및 인체 기관에서의 각종 대사물질의 정량적인 양과 변화를 알 수 있는 자기공명분광기법(magnetic resonance spectroscopy:MRS)도 활발한 임상적용이 이루어지고 있다. 자기공명영상법의 활발한 임상적 응용 및 새로운 technique이해를 위해서는 물리학적 개념(MR Physics)을 이해하는 것이 매우 중요하다.

• Korean Journal of Veterinary Service
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• v.42 no.4
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• pp.305-311
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• 2019

The objective of this study was to determine the safety test for propolis in Beagle based on blood count, serum biochemistry, and electrolyte. Total six beagle dogs were assigned to this experiment. To investigate the safety of propolis in beagle dogs, we performed oral administration of propolis (5%) for 8 weeks. Among six beagles, three beagle dogs were randomly allocated to the control group which were fed only regular fodder, and the other three dogs were assigned as the treatment group which were fed regular fodder and propolis (5%). No clinical signs were observed in neither group throughout the experimental period. During the experimental period, there were no significant change in feed intake, water consumption, and body condition. Also, there were no statistically significant differences in hematological and biochemical analyses between the control group and the treated group. Our safety study showed that oral consumption of propolis did not cause any toxicological effects in beagle dogs.