• Korean Journal of Environmental Biology
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• v.29 no.1
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• pp.61-67
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• 2011

N-acetyl-L-cysteine (NAC) having a thiol, a precursor for glutathione (GSH), is known as one of the antioxidants. NAC used as a radioprotector against ionizing radiation (IR)-induced injury and damage. The aim of this study was to evaluate the radioprotective effects of NAC against IR-induced cell damage in Saccharomyces cerevisiae and the antioxidative effect of NAC on transcriptional level of yeast antioxidant enzyme genes such as superoxide dismutase (SOD) and catalase. In the present study, yeast cells were pretreated with various concentrations of NAC and/or irradiated with various doses of gamma rays. The cell viability was measured by counting the cell forming unit (CFU). The quantitative real-time PCR was performed for analysis of gene expression of SOD and catalase. The viability of irradiated cells was not improved by pretreatment with NAC. Ionizing radiation with 100 Gy highly induced the gene expression of antioxidant enzymes. In the irradiated group with NAC pretreatment, the gene expression of SOD and catalase was gradually reduced with the increased concentrations of NAC. These results indicate that NAC can act as a useful antioxidant to scavenge reactive oxygen species in vivo, but does not protect cells against IR-induced cell death in S. cerevisiae.

• Proceedings of the Korea Society of Information Technology Applications Conference
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• 2006.06a
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• pp.495-505
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• 2006

IT기술기반 교육시스템은 인터넷 등장 이전에도 가능성을 인정받아 지속적으로 개발되어 온 분야이며, 교육공학과의 연계로 지식 전날의 이론체계로 각광을 받고 있다. 사이버교유도 인터넷이전부터 다양한 통신방법을 응용하여 개발되었고, 최근 인터넷을 통하여 사이버 교육시스템은 완벽한 기술기반을 갖추게 되였다. 그러나 IT기술의 급격한 변화로 사이버교육시스템은 계속하여 신기술 변화에 적용해야만 한다. 현재 정보통신기술의 변화는 방송 통신망의 융합, 브로드 밴드 네트워킹, 스마트 디바이스의 다양화, 멀티미디어 기술의 고도화로 요약된다. 이 기술의 종합한 작용으로 유비쿼터스 사회의 기반으로 진화되고 있다. 그래서 e-Learning 분야도 기존 인터넷기반 시스템과는 달리 차세대 온라인교육시스템으로 친화되고 있다. IT융합가술 기반의 온라인 교육시스템은 각종 국제표준단체에서 표준안이 제시되고 있다. e-Learning 시스템이란 선기술 기반을 반영한 표준기술을 사용하는 온라인교육시스템을 포괄하는 개념이다. 본 연구에서는 e-Learning 시스템과 유비쿼터스 기술을 반영한 e-Learning을 비교하였다. 그리고 u-Learning 시스템의 기술정립과 EOD(Education On Demand) 시스템에 대하여 연구하였다. 1. u-Learning 정의 정보산업분야를 비롯한 문화, 교육 등 모든 분야에서 유비퀴터스라는 수식어가 붙어 다니고 있다. e- Learning 교육 업계에 따르면 10년 후에는 유비쿼터스는 대중화가 될 것이며, 부가가치 규모는 100조 원에 이를 것으로 추정된다. 그래서 교육산업도 주변 환경이 아날로그 방식에서 IT 기반에 의한 디지털 환경으로 변화되고 있다. 또한 e러닝, T러닝, m러닝, u러닝 등의 용어가 생성되고 있다.키지에어컨에서 사용되고 있는 밀폐형 압축기에 대해서 그림 2에서 나타내고 있는 냉방능력 10tons(120,000Btu/h) 이하를 중심으로 상기의 최근 기술 동향을 간략하게 소개하고자 한다.질표준의 지표성분으로 간주되는 진세노사이드의 절대함량과 그 성분조성 차이에 따른 임상효과의 차별성이 있는지에 대한 검토와, 특히 최근 실험적으로 밝혀지고 있는 사포닌 성분의 장내 세균에 의한 생물전환체의 인체 실험을 통한 효과 검정이 필요하다. 나아가서는 적정 복용량의 설정과 이와 관련되는 생체내 동태 및 생체이용율(bioavilability)에 관한 정보가 거의 없으므로 이것도 금후 검토해야 할 과제로 사료된다. 인삼은 전통약물로서 오랜 역사성과 그동안의 연구결과에 의한 과학성을 가지고 있으므로 건강유지와 병의 예방 및 회복촉진을 위한 보조요법제 또는 기능성 식품으로써의 유용성이 있는 것으로 판단된다. 앞으로 인삼의 활용성 증대를 위해서는 보다 과학적인 임상평가에 의한 안전성 및 유효성 입증과 제품의 엄격한 품질관리의 필요성이 더욱 강조되어야 할 것이다.xyl radical 생성 억제 효과를 보여 주었다. 본 실험을 통하여 BHT 를 제외하고 전반적으로 세포 수준에서의 oxidative stress 에 대한 억제 효과를 확인해 볼 수 있었으며 특히 수용성 항산화제들에서 두드러진 효과를 보여 주었다. 제공하여 내수기반 확충에도 노력해야 할 것 이다.있었다., 인삼이 성장될 때 부분적인 영양상태의 불충분이나 기후 등에 따른 영향을 받을 수 있기 때문에 앞으로 이에 대한 많은 연구가 이루어져야할 것으로 판단된다.태에도 불구하고 [-wh]의미의 겹의문사는 병렬적 관계의 합성어가 아니라 내부구조를 지니지 않은 단순한 단어(minimal $X^{0}$<

• Journal of Nutrition and Health
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• v.54 no.5
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• pp.448-458
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• 2021

Purpose: Mulberry (Morus alba L.) fruit is widely grown in Asia and consumed as fresh fruit, jam, and juices. The fruit has beneficial health effects, including anti-diabetic, anti-tumor, and anti-obesity properties. However, the mechanisms by which mulberry fruit juice powder (MJ) regulates inflammatory microRNAs (miRs) are not yet known. This study investigated the effect of mulberry fruit juice powder on the regulation of inflammation and miR-132/143 during 3T3-L1 adipocyte differentiation. Methods: The 3T3-L1 cells were induced to differentiate for 2 days and then treated with various concentrations of MJ for 7 days. Cytotoxicity was determined by evaluating cell viability using a water-soluble tetrazolium salt-8 assay kit. Intracellular lipid accumulation was evaluated by oil-red O staining. The levels of the expression of genes involved in adipogenesis and inflammation, and miR-132/143 were measured by quantitative real-time polymerase chain reactions. Results: MJ showed no cytotoxic effect on 3T3-L1 adipocytes at concentrations below 100 ng/mL. Intracellular lipid accumulation was reduced by MJ treatment at concentrations of 100 ng/mL. The messenger RNA (mRNA) levels of proliferator-activated receptor-γ, cytosine-cytosine-adenosine-adenosine-thymidine/enhancer-binding protein-α, and adipocyte protein 2, which are involved in adipogenesis, were suppressed by MJ. A reduction was also seen in mRNA levels of genes related to the inflammatory response, such as tumor necrosis factor-α, interleukin-6, monocyte chemoattractant protein-1, and inducible nitric oxide synthase. The expression of the inflammatory miR-132 and miR-143 was also decreased by MJ. Conclusion: These results suggest that MJ may suppress adipogenesis and inflammation through the regulation of miR-132/143 expression in 3T3-L1 adipocytes. Thus, MJ may be useful as a food agent that prevents obesity-associated inflammation.

• Analytical Science and Technology
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• v.22 no.5
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• pp.386-394
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• 2009

Formaldehyde is used in lether manufacture, a dry plate and an explosive. It is by-product of ozonizing process in filtration plant. The effects of exposure are eye pruritus, tickle, runing nose, blocking nasal passages and headache. It also makes a dried throat and causes inflammation. It is classified as B1 group for inhalation by US. EPA, which can cause cancer in human. For analysis of formaldehyde, formaldehydes-DNPH derivative was extracted with solid cartridge and was analyzed by High Performance Liquid Chromatography/Diode Array Detector (HPLC/DAD). The detection limit was $3{\mu}g/L$ and the recoveries were 72.3~109.1% (RSD 2.9~11.5%). Water samples were collected in four Korean rivers, four times per year seasonally for 10 years from 1998 to 2007. The monitoring results were 48.8% (630/1291), $5.15{\sim}101.9{\mu}g/L$ in purified water. Because of non-carcinogen in drinking water, hazard index is calculated with RfD. Results of excess cnacer risk was below 1 and was considered as safe value.

• Journal of the East Asian Society of Dietary Life
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• v.26 no.1
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• pp.88-98
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• 2016

The pharmacological effects of ginseng berry have been known to improve psychological function, immune activities, cardiovascular conditions, and certain cancers. It is also known that fermentation improves the bioavailability of human beneficial natural materials. Accordingly, we investigated the optimal fermentation conditions of ginseng berry extract with strain isolated from conventional foods. We also analyzed the fermentation product and its antioxidant activity. The bacterium isolated from fermented kimchi was identified as Lactobacillus sp. strain KYH. To optimize the process, fermentation was performed in a 5 L fermenter containing 3 L of ginseng berry extract at 200 rpm for 72 hr. Under optimized conditions, batch and fed-batch fermentations were performed. After fermentation, organic acids, amino acids, sugars, ginsenosides, and antioxidant activity were evaluated. The optimum fermentation conditions were determined as pH 7.0 and a temperature of $30^{\circ}C$, respectively. After fermentation, the amounts and compositions of organic acids, amino acids, sugars, ginsenosides, and antioxidant activity were altered. In comparing the distribution of ginsenosides with that before fermentation, the ginsenoside Re was a major product. However, amounts of ginsenosides Rb1, Rc, and Rd were reduced, whereas amounts of ginsenosides Rh1 and Rh2 increased. Total phenol content increased to 43.8%, whereas flavonoid content decreased to 19.8%. The DPPH radical scavenging activity and total antioxidant activity increased to 27.2 and 19.4%, respectively.

• Journal of Life Science
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• v.29 no.6
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• pp.653-661
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• 2019

The first small interfering RNA (siRNA) therapeutics have recently been approved by the Food and Drug Administration in the U.S., and the demand for a new RNA therapeutics bioanalysis method-which is essential for pharmacokinetics, including the absorption, distribution, metabolism, and excretion of siRNA therapeutics-is rapidly increasing. The stem-loop real-time qPCR (RT-qPCR) assay is a useful molecular technique for the identification and quantification of small RNA (e.g., micro RNA and siRNA) and can be applied for the bioanalysis of siRNA therapeutics. When the anti-HPV E6/E7 siRNA therapeutic was used in preclinical trials, the established stem-loop RT-qPCR assay was validated. The limit of detection was sensitive up to 10 fM and the lower limit of quantification up to 100 fM. In fact, the reliability of the established test method was further validated in three intra assays. Here, the correlation coefficient of $R^2$>0.99, the slope of -3.10 ~ -3.40, and the recovery rate within ${\pm}20%$ of the siRNA standard curve confirm its excellent robustness. Finally, the circulation profiles of siRNAs were demonstrated in rat serum, and the pharmacokinetic properties of the anti-HPV E6/E7 siRNA therapeutic were characterized using a stem-loop RT-qPCR assay. Therefore, the stemloop RT-qPCR assay enables accurate, precise, and sensitive siRNA duplex quantification and is suitable for the quantification of small RNA therapeutics using small volumes of biological samples.

• Smart Media Journal
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• v.3 no.3
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• pp.15-19
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• 2014

Fingerprint Recognition System is made up of Off-line treatment and On-line treatment; the one is registering all the information of there trier features which are retrieved in the digitalized fingerprint getting out of the analog fingerprint through the fingerprint acquisition device and the other is the treatment making the decision whether the users are approved to be accessed to the system or not with matching them with the fingerprint features which are retrieved and database from the input fingerprint when the users are approaching the system to use. In this paper, Among various biometrics recognition systems, statistical fingerprint recognition matching methods are considered using minutiae on fingerprints. We define similarity distance measures based on the coordinate and angle of the minutiae, and suggest a fingerprint recognition model following statistical distributions.

• Analytical Science and Technology
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• v.14 no.5
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• pp.400-409
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• 2001

A method for the analysis of most common phthalate acid esters (9 secies) in biota samples by gas chromatography-mass spectrometry-selected ion monitoring mode is described. Phthalates in biota samples are extracted by organic solvent and purified by Florisil column. Phthalates are easily contaminated during extraction prodedure. Since the extraction and cleanup steps for biota samples generally are more complicate than those for water or sediment samples, we compared with contamination state of each sample work-up step. By applying this developed method, the overall recoveries ranged between 79 - 117% in biota sample which was spiked with standards. For phthalates used in this study, the quantitaive accuracy, elution pattern on Florisil column, and detection limits were also investigated.

• Journal of Nutrition and Health
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• v.20 no.2
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• pp.104-110
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• 1987

Thirty mael Sprague Dawley rats of 3 weeks of age were adopted to investigate changes in nutritive quality of the traditional, fermented soybean foods, Meju and Chungkugjang prepared by culturing with Aspergillus oryzae and the rice straw, respectively. The levels of each dietary protein were set at 10% . The effect of supplementation of fermented soybean on the rat diet was evaluated by measuring growth rate, feed efficiency. weight of the pancreas and biological values, such as NPU, PER and NPR on day 10. After incubation of soybean with Aspergilllus oryzae and the rice straw, the contents of crude protein, crude fat and crude ash were increased, but the levels of crude fiber and nitrogen-free extract were reduced. The amounts of feed intake, body weight gain an feed efficiency were greater for the rats fed dietscontaining heated unfermented soybeans or Meju compared to rats fed the diet containing raw soybeans. Pancreating enlargement was observed in the rats fed diets containing raw soybeans. The values of NPR and PER in rats fed diets containing Meju and Chungkugjang were significantly higher than those of rats fed diets containing raw soybeans. The values of NPU in rats fed diets containing raw soybans, heated unfermeted soybeans. Meju and Chungkugjang were 39.40%, 40.60%, 45.00% and 46.205, respectively, demonstrating no significant differences.

• Proceedings of the Korea Information Processing Society Conference
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• 2005.05a
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• pp.789-792
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• 2005

최근 급격히 발전한 휴대폰은 다양한 기능을 가지고 있다. 그 중 디지털 카메라의 기능을 겸비한 휴대폰은 디지털 카메라의 판매량을 앞서고 있고, 메가픽셀의 고화소 디카폰의 개발로 대중화가 더욱 가속화되고 있다. 카메라폰을 응용한 연구분야로는 생체인식기술을 적용할 수 있으며, 본 논문은 제약이 많은 휴대폰 환경에서 홍채인식기술을 적용하기 위한 휴대폰 카메라로 취득된 얼굴영상에서의 눈 영역을 검출하는 방법을 제안한다. 얼굴영상에서 눈은 피부나 머리카락보다 빛에 대한 반사율이 높아 각막에 specular reflection이 생기게 되고, 동공은 눈의 다른 지역에 비해 흑화소가 많다는 특징을 가지고 있다. 이러한 두 가지 특징을 이용하여 동공 후보 영역을 선정하였고, 선정된 이진영상에서 수평 프로파일과 수직 프로파일을 적용하여 동공 후보 영역을 줄이면서 동공의 중심 위치를 검출한다. 본 연구는 휴대폰 환경을 고려하였기 때문에 최소한의 메모리 사용과 적은 연산량을 목표로 하여 눈의 위치를 검출 한다. 실험 결과, 입력 영상 내에 일정크기의 동공영역이 존재할 경우 높은 눈 영역 추출 성공률을 보이며, 본 연구에서 제안한 알고리즘을 실제 휴대폰에서 수행한 결과 평균571.6ms의 시간이 소요됨을 알 수 있었다.