• Journal of the Korean Society of Food Science and Nutrition
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• v.33 no.1
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• pp.66-71
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• 2004

This study has been carried out to investigate effect of Rehmanniae Radix (RR) and pear phenolic compound (PC) on the hyperglycemic mice induced with streptozotocin (STZ). For this purpose, male mice were fed with a 0.2 mL RR extract (S group) and the pear PC (90 mg/kg/day) dissolved in a 0.2 mL RR extract (SPC group) while the control group received the same commercial diet for 6 weeks. The blood glucose contents were examined from tail vein blood once a week for 6 weeks. Samples of pancreas removed after the experimental period were processed for the immunohistochemical identification of $\beta$-cells. The levels of serum glucose were decreased significalntly (p<0.05) in the S and SPC groups compared with the control group. The BUN and creatinine levels were significantly (p<0.05) decreased in SPC group compared with the control group. Intraperitoneal glucose tolerance tests peformed at 24 hours before that period revealed that glucose tolerances in S and SPC group were ameliorated. Immunohistochemical analyses of the pancreases revealed that a lot of insulin- positive $\beta$-cells were contained in a Langerhas's islets of S and SPC groups compared with the control group, and the number of Langerhas's islets were significalntly increased in S (p<0.01) and SPC (p<0.05) groups. These results suggest that RR extract and pear PC could recover the damages induced by STZ in the hyperglycemic mice.

• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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• v.5 no.4
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• pp.285-294
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• 2000

In order to determine the horizontal and vertical distributions of metals and prospect the recent metal pollution history in Nakdong Estuary, we took surface and core sediments. Maximum value of organic matter occurs at the upstream site located 4 km from Nakdong barrage, and the concentration of trace metals (Zn, Cu, and Pb etc.) decrease seaward in the estuary. The sedimentation rates, based on $^{210}$Pb$_{ex}$ and $^{137}$Cs activities, were 0.34 cm/yr in inside of barrage (core 1) and 0.25 cm/yr in Changrim (core 4). Sediment mixing layer does not exist in core 1, where anoxic condition is known to be prevail. The topmost sediment layer of core 4 (<3.5 cm) is severely mixed. At sites 1 and 4, concentrations of Cu slowly increased during the period of 1920-1970, rapidly increased during 1970-1990, and followed by slight decrease after 1990. Zn contents increased in early 1960s and peaked in 1993, and followed by decrease after 1990s. Pb has increased continuously since early 1970s. At the downstream of the barrage, Cu and Zn have increased in the topmost layer. The trend of increase of Cu is evident after 1950 (11 cm in sediment depth). Overall trend of heavy metal concentration clearly indicates the pollution has been increasing after the construction of the barrage.

• The korean journal of orthodontics
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• v.32 no.1 s.90
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• pp.19-31
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• 2002

To investigate the association between the hypertrophy of adenoid and jaw morphology during growth, this paper was based on children patients with experimental adenoids (male-15 subjects at each bone age group, female-15 subjects at each bone group) and comparing them to data taken from a control group (male-15 subjects at each bone age group, female-15 subjects at each bone group) with normal respiratory function. The comparisons between the groups were done at each growth stage using cervical vertebrae maturation index(CVMI) of Hassel. The obtained results were as follows : 1. The differences in craniofacial morphology between experimental group and control group were appeared from CVMI 3 and CVMI 4 (aroud adolescent period) in males, and from CVMI 1 in females. 2. The mandibular position of experimental group was more inferior than control group. The difference appeared at adolescent period(male : at CVMI 4, female : at CVMI 5). 3. Experimental group had greater anterior facial height than control group. This difference seemed a relation with lower anterior facial height. The difference appears at CVMI 3(11.94 ${\pm}$ 1.38 years old, at adolescent period) in male and at CVMI 1 in female. 4. The adenoid size of control male group was increased until CVMI 2(10.58 ${\pm}$ 1.07 years old, just before adolescent growth peak) and then decreased, but in female the adenoid size was decreased from CVMI 1(6.92 ${\pm}$ 0.53 years old).

• Journal of Wetlands Research
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• v.12 no.1
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• pp.1-14
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• 2010

Changes in wetland soil properties of two constructed wetlands after their constructions were compared to those of a natural wetland to determine if they could be used for the evaluation of the success of constructed wetlands and the assessment of their functions. One natural wetland as a reference wetland and two constructed wetlands(treatment wetland and experimental wetland) with different contaminant inflow characteristics were selected for this study. Major physicochemical properties of wetland soil such as soil texture, water content, pH, CEC(cation exchange capacity), organic matter content, total nitrogen, and available phosphorus were monitored to investigate the effects of inundation and accumulation of organic matters and nutrients on the wetland soil development. There was a clear difference in soil texture between the natural wetland and the constructed ones, with the high sand content in the constructed wetlands as compared to the high clay content in the natural one. Gradual increases of silt and clay contents over time were observed in the constructed wetlands. The soil of the natural wetland was higher in water content and organic matter but lower in pH than those of the constructed wetlands. The pH of the constructed wetlands reached near neutral ranges after initial increase. CEC and nutrient concentrations of the constructed wetlands seemed to be affected mainly by outside inflows of organic matter and contaminants. Concentrations of organic matter and nutrients decreased over time in the experimental wetland where surface and deep soils with different characteristics were mixed during its construction, suggesting that changes in soil properties during wetland constructions may affect the development of wetland soils or wetland biogeochemistry. This study showed that changes in physicochemical properties of soils in constructed wetlands could be used to assess the success of constructed wetlands and their functions, and also the importance of reference wetlands for the appropriate assessment.

• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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• v.2 no.2
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• pp.117-124
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• 1997

Vertical profiles of dissolved Cu and Ni at the upper 400 m water layer have been determined at two stations in the central East Sea in October 1995. This is the first report on the vertical distribution of trace metals in Korea. Copper concentrations are in the range of 2.1~5.8 nmol/kg and 1.6~2.4 nmol/kg for stations S and N, respectively. The vertical profile of Cu at S is found a scavenging type (i.e., drastic decrease with increasing depth). Concentrations of Ni range from 4.3 to 7.1 nmol/kg and from 3.4 to 5.4 nmol/kg for stations Sand N, respectively. At station S, Ni is best correlated with phosphate, but not at stations N. Such difference between two stations are probably due to their different vertical distribution of water masses. Station S has a strongly stratified water column with 6 distinct water masses, but station N with a well-mixed subsurface water layer extending from 50 to 300 m depth. Extremely low salinity (31.87~31.96 psu) found at the surface water of station S was interpreted as a result of the Yangtze River effluents which were probably fed into the East Sea through the Korea Strait during the late summer. Such seasonal appearance of low salinity in southern part of the East Sea was reported previously. The concentrations of Cu and Ni at two sites are comparable to those reported in the North Pacific. It was found that Ni mostly exist as dissolved phase.

• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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• v.23 no.1
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• pp.32-48
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• 2018

The mechanisms of $CH_4$ uptake into and release from the ocean are not well understood due mainly to complexity of the biogeochemical cycle and to lack of regional-scale and/or process-scale observations in the marine boundary layers. Without complete understanding of oceanic mechanisms to control the carbon balance and cycles on a various spatial and temporal scales, however, it is difficult to predict future perturbation of oceanic carbon levels and its influence on the global and regional climates. High frequency, high precision continuous measurements for carbon isotopic compositions from dissolved $CH_4$ in the surface ocean and marine atmosphere can provide additional information about the flux pathways and production/consumption processes occurring in the boundary of two large reservoirs. This paper introduces recent advances on optical instruments for real time $CH_4$ isotope analysis to diagnose potential applications for in situ, continuous measurements of carbon isotopic composition of dissolved $CH_4$. Commercially available, three laser absorption spectrometers - quantum cascade laser spectroscopy (QCLAS), off-axis integrated cavity output spectrometer (OA-ICOS), and cavity ring-down spectrometer (CRDS) are discussed in comparison with the conventional isotope ratio mass spectrometry (IRMS). Details of functioning and performance of a CRDS isotope instrument for atmospheric ${\delta}^{13}C-CH_4$ are also given, showing its capability to detect localized methane emission sources.

• Journal of Wetlands Research
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• v.8 no.4
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• pp.41-47
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• 2006

Global climatic changes are expected to influence various biogeochemical processes in wetland ecosystems. In particular, coastal mud flat is anticipated to be affected directly by temperature increase as well as indirectly by a sea level rise and changes in precipitation. This study aimed to determine changes in methane production under different temperature and salinity by employing a laboratory-scale manipulation experiment. Soil samples were collected from a mud flat in Dong-Gum Kang-Hwa island in winter and two types of experiments were conducted. In the first experiment soil samples at 0-5 cm, 5-10 cm depth were incubated under same salinity with pore water and diluted salinity to 50 % of natural condition for 20 days and methane production was measured every other days. In the second experiment, soil samples at 5-10 cm depth were incubated in different temperature, $5^{\circ}C$ and $15^{\circ}C$, under same salinity conditions with first experiment for 31 days and methane production was measured. Results of the first experiment revealed that higher amount of methane was produced at 5-10 cm depth, and salinity effect was predominant at the end of the experiment. The second experiment showed that methane production was higher in $15^{\circ}C$ than $5^{\circ}C$. In addition, methane production was higher when sea water diluted to 50 % compared to control. Global climatic changes are expected to influence various biogeochemical processes in wetland ecosystems. In particular, coastal mud flat is anticipated to be affected directly by temperature increase as well as indirectly by a sea level rise and changes in precipitation. This study aimed to determine changes in methane production under different temperature and salinity by employing a laboratory-scale manipulation experiment. Soil samples were collected from a mud flat in Dong-Gum Kang-Hwa island in winter and two types of experiments were conducted. In the first experiment soil samples at 0-5 cm, 5-10 cm depth were incubated under same salinity with pore water and diluted salinity to 50 % of natural condition for 20 days and methane production was measured every other days. In the second experiment, soil samples at 5-10 cm depth were incubated in different temperature, $5^{\circ}C$ and $15^{\circ}C$, under same salinity conditions with first experiment for 31 days and methane production was measured. Results of the first experiment revealed that higher amount of methane was produced at 5-10 cm depth, and salinity effect was predominant at the end of the experiment. The second experiment showed that methane production was higher in $15^{\circ}C$ than $5^{\circ}C$. In addition, methane production was higher when sea water diluted to 50 % compared to control. These results suggest that methane production is highly influenced by changes in temperature and salinity in coastal mud flat. And that global climatic change may induce biological feedback by affecting production of another greenhouse gas, namely methane from coastal mud flat.

• Korean Journal of Ecology and Environment
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• v.36 no.4 s.105
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• pp.455-462
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• 2003

This study evaluated the change of water quality and chlorophyll - a at the birth stage of a large reclaimed estuarine lake (Lake Hwaong) of which the dike was finally constructed in March, 2002. Physico -chemical parameters and chlorophyll - a were investigated along a longitudinal transect, including 3 in-lake sites and 1 out-lake site from June to November, 2002. Salinity at all in-lake sites was over 21 psu during the study period, indicating that lake is still in the seawater phase. Salinity was periodically diluted at times when precipitation was high, especially in August. Chemocline was established in July near the dam site, and correspondingly vertical profile of dissolved oxygen was very clear during that Period. Total nitrogen and phosphorus concentrations at all lake sites were in the eutrophic range, and they were especially high at the stream inlet site. Nutrients concentration was not much varied vertically but significantly varied temporally, and correlated significantly with precipitation and chlorophyll-a concentration, indicating that inflowing water from the watershed seemed not to improve lake water by dilution but cause eutrophication of the lake, and thereby stimulate phytoplankton development. Based on the analyses of nutrient ratio (N/P) and trophic state deviation, both phosphorus and nitrogen appeared to limit phytoplankton growth in the lake. Phosphorus limitation appeared to be probable at all in-lake sites with being most severe at the stream inlet site. Nitrogen limitation seemed to occur at both in-lake and out-lake sites. These results indicate that in Lake Hwaong experiencing the very early stage of a reclaiming lake, water quality and phytoplankton development appear to be affect-ed largely by salinity and hydrology and nutrients from the inflowing water. Lake biogeochemistry is still very unstable, and thus further long-term study is necessary to understand the effects of seawater to freshwater conversion on lake biology and water chemistry.

• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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• v.11 no.2
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• pp.49-67
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• 2006

A mesocosm, an artificial tidal flat ecosystem, was constructed outdoors to simulate in situ physical and biochemical environmental conditions of natural tidal flat as much as possible. During the experiment from February to August 2004, the study was focused on the biogeochemical variations of superficial water and porewater after introduction of freshwater into the mesocosm. The mesocosm has three experimental conditions; SW-M-T: maintaining the saline water of approximately 20 psu; FW-M-T: complete exchange of freshwater ul the mesocosm with continuous mixing of water column: FW-NM-T: complete exchange of saline water to freshwater in the mesocosm without mixing of water column. Mass extinction of benthic macrofauna appeared due to drastic decrease of porewater salinity from 20 psu to less than 10 psu between the 63th and 91st day of freshwater introduction in FW-M-T and FW-NM-T. Throughout the periods, 7/8 of bivalves and 2/3 of polychaete populations have been extinguished in the sediment. In FW-NM-T, as temperature rises, both evident decrease of DO in water column and active release of DIP from sediment were observed. ${NO_3}^-$ was removed from water column into sediment throughout the periods. Therefore extremely low ${NO_3}^-$ was found during late spring and summer. Whereas ${NH_4}^+$ exhibited only $1/2{\sim}1/8$ of ${NO_3}^-$ concentration. Unexpectedly even after mass extinction of benthic macrofauna, we were not able to find high ${NH_4}^+$. This mesocosm study suggests that when fresh water introduce to natural tidal flat, its sediment activity functions as a potential source of DIP, but a sink of ${NO_3}^-$.

• Journal of Korean Society of Disaster and Security
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• v.16 no.4
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• pp.45-59
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• 2023

The global focus on mitigating climate change has traditionally centered on carbon dioxide, but recent attention has shifted towards methane as a crucial factor in climate change adaptation. Natural settings, particularly aquatic environments such as wetlands, reservoirs, and lakes, play a significant role as sources of greenhouse gases. The accumulation of organic contaminants on the lake and reservoir beds can lead to the microbial decomposition of sedimentary material, generating greenhouse gases, notably methane, under anaerobic conditions. The escalation of methane emissions in freshwater is attributed to the growing impact of non-point sources, alterations in water bodies for diverse purposes, and the introduction of structures such as river crossings that disrupt natural flow patterns. Furthermore, the effects of climate change, including rising water temperatures and ensuing hydrological and water quality challenges, contribute to an acceleration in methane emissions into the atmosphere. Methane emissions occur through various pathways, with ebullition fluxes-where methane bubbles are formed and released from bed sediments-recognized as a major mechanism. This study employs Biochemical Methane Potential (BMP) tests to analyze and quantify the factors influencing methane gas emissions. Methane production rates are measured under diverse conditions, including temperature, substrate type (glucose), shear velocity, and sediment properties. Additionally, numerical simulations are conducted to analyze the relationship between fluid shear stress on the sand bed and methane ebullition rates. The findings reveal that biochemical factors significantly influence methane production, whereas shear velocity primarily affects methane ebullition. Sediment properties are identified as influential factors impacting both methane production and ebullition. Overall, this study establishes empirical relationships between bubble dynamics, the Weber number, and methane emissions, presenting a formula to estimate methane ebullition flux. Future research, incorporating specific conditions such as water depth, effective shear stress beneath the sediment's tensile strength, and organic matter, is expected to contribute to the development of biogeochemical and hydro-environmental impact assessment methods suitable for in-situ applications.