• Reproductive and Developmental Biology
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• v.30 no.3
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• pp.219-224
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• 2006

The objective of this study was to investigate the toxic effects of Bisphenol A(BPA) and di-2 ethyhexyl phthalate (DEHP) as endocrine disrupters on sperm motility, viability, membrane integrity and abnormality during in vitro incubation of boar semen. Semen were randomly divide into 24 groups and healed with different concentrations of BPA md DEHP($1{\sim}100{\mu}M$) for 3, 6 and 9 hrs, respectively. The percentages of sperm motility and viability decreased by treatment time with both BPA and DEHP, and obiously differ from the controls. The percentages of sperm motility and viability significantly decreased by incubation with both $100{\mu}M$ of BPA and DEHP compared to control and other treatment groups(p<0.05). Sperm membrane integrity was significantly reduced by incubation with 10 and $100{\mu}M$ of BPA and DEHP, respectively(p<0.05), but sperm abnormality were not significantly affect both BPA and DEHP. These results indicate that high concentration of BPA and DEHP($>10{\mu}M$ can affect noxiously the sperm characteristics.

• Korean Journal of Food Science and Technology
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• v.54 no.2
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• pp.203-208
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• 2022

Gluten is an insoluble protein present in cereals such as wheat. Gluten consumed through food is not digested and accumulates in the body; this has been linked to digestive discomfort, irritation, and various digestive disorders, including intestinal inflammation. In this study, the Lactobacillus paracasei strain GLU70, which exhibits a glutendegrading ability, was isolated from salted seafood. At a pH of 3.0, GLU70 showed a survival rate of approximately 84%, and at 0.3% oxgall, it showed a survival rate of approximately 53%. When the culture supernatant collected after 12 h of incubation was added to flour dough, approximately 50% gluten degradation was observed. Moreover, among several probiotic isolates exhibiting proteolytic activity selected to assess the gluten-degrading ability, GLU70 showed superior results regardless of the dough fermentation temperature. Although further research is required, GLU70 is expected to be of value in manufacturing gluten-reduced products and the food industry as an ingredient or additive.

• Journal of the Korean Society for Aeronautical & Space Sciences
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• v.47 no.8
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• pp.558-565
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• 2019

A lot of studies on the survivability of missiles have been widely studied, since the technology of modern warships equipped with state-of-the-art defense systems has been improved. The survivability of missiles can be improved by attacking a target simultaneously using multiple missiles. For this reason, impact time control guidance (ITCG) laws have been widely studied. This paper deals with the effect of time-to-go estimate to ITCG laws. In this paper, two kinds of time-to-go estimate are first introduced in two-dimensional and three-dimensional environment and then ITCG laws are derived using the time-to-go estimate. Numerical simulations are performed to analyze the performance of the designed ITCG laws and the effect of time-to-go estimate is discussed.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.30 no.4D
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• pp.323-330
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• 2010

The time in port for vessels is one of the important factors for analyzing the operation status and the capacity of ports. In addition, the time in port for vessels can be directly used for estimating the greenhouse gas emissions resulted from vessels in port. However, it is unclear which variables can affect the time in port for vessels and what the marginal effect of each variable is. With these challenges in mind, the study analyzes the time in port for vessels arriving and departing port of Busan by using a parametric survival model. The results show that the log-logistic accelerated failure time model is appropriate to explain the time in port for 19,167 vessels arriving and departing port of Busan in 2008, in which the time in port is significantly affected by gross tonnage of vessels, service capacity of terminal, and vessel type. This study also shows that the greenhouse gas emission resulted from full-container vessels, which accounted for about 61% of all vessels with loading/unloading purpose arriving and departing port of Busan in 2008, is about "17 ton/vessel" in the boundary of port of Busan. However, the hotelling greenhouse gas emissions resulted from non-container vessels (3,774 vessels; 20%) are greater than those from the full-container vessels. Hence, it is necessary to take into account more efficient port management polices and technologies to reduce the service time of non-container vessels in port of Busan.

• Journal of Aquaculture
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• v.19 no.4
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• pp.305-309
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• 2006

This study was carried out to examine the anesthesia and recovery time of the male and gravid female of marine benthic copepod Tigriopus japonicus at 500, 750, 1,000 ppm of MS-222. We also investigated the survival (%) and the nauplii production of T. japonicus for 14 days, which was anesthetized with MS-222. As the concentration of MS-222 was higher, the anesthesia time of the copepod was shorter and the recovery time was longer. The survival (%) of male T. japonicus was significantly higher than that of the gravid female both in anesthetized and control group. But the survival (%) of the male treated with MS-222 did not show the different survival (%) with the control (0 ppm). In contrast, the survival (%) of the gravid female treated with 750 and 1,000 ppm was significantly lower than that of 500 ppm and control. With regard to hatching, as the concentrations was higher, the number of hatching and total nauplii were lower and interval of hatching was longer. However, the gravid female treated with 500 ppm did not show the significant difference with control. We suggest that 500 ppm of MS-222 is the suitable concentration in isolation of T. japonicus.

• Journal of Dairy Science and Biotechnology
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• v.30 no.1
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• pp.45-53
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• 2012

Lactobacillus acidophilus isolated from the feces of a 7-day-old breast-fed infant was characterized to examine the scope of its commercial use. Forty-three Lactobacillus strains, which could grow at pH 5.5, were isolated. From these Lactobacillus isolates, 14 Lactobacillus strains were selected, which demonstrated more than 80% viability and homofermentative lactic fermentation. Finally, 9 L. acidophilus strains (NB 201~NB 209) were identified as candidate strains based upon biochemical properties, carbohydrate utilization, and cellular fatty acid composition. L. acidophilus isolates demonstrated a survival rate of more than 80% when exposed to pH 2.5 for 2 h. In particular, L. acidophilus NB 204 showed a strong acid tolerance, with a 71% survival rate even at pH 2.0. L. acidophilus isolates also manifested high bile acid tolerance; more than 87% of the cells survived on agar containing 1% bile extract, except for L. acidophilus NB 206, which showed a 73% survival rate. All L. acidophilus isolates were confirmed to have proteolytic activity; L. acidophilus NB 204 and NB 209 yielded higher levels of TCA-soluble peptides and free amino acids. The ${\beta}$-galactosidase activity of the L. acidophilus isolates was in the range of 1.97~2.45 units/mL.

• Korean Journal of Animal Reproduction
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• v.15 no.1
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• pp.49-57
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• 1991

This study was carried out to investigate the effect of equilibration time, precooling and straw loading method on the post-thaw survival rates of mouse embryos cryopreserved by vitrification method. The effect of the vitrification procedure on the damage of the embryos was also examined by the straining of nuclei using Hoechst 33342. The results obtained were summarized as follows; 1. The equilibration in Medium-1 for 10 minutes was considered to be the optimum equilibration time. Embryos equilibrated in Medium-1 for 10 minutes(81.0%) showed significantly(P<0.05) higher survival rates than those equilibrated for 5 minutes(40.0%) or 15 minutes(74.1%). 2. The survival rate of embryos cryopreserved using the double Medium-2 column(81.0%) was significantly(P<0.01) higher than that using the single Medium-2 column, whish was considered to be due to the double Medium-2 column method being more reliable for preventing contamination of diluent solution of 1M sucrose. 3. The survival rate of compacted morula stage embryos cryopreserved with the precooled and non-precooled Medium-2 was not significantly(P<0.05) different. 4. The number of blastomeres of embryos at late blastocyst stage after culture of mouse morulae for 24 to 28hours was significantly(P<0.05) decreased by freezing embryos using vitrification(53.3${\pm}$1.6 vs 41.4${\pm}$1.5), which was considered to be due to the damage of embryos during vitrification and the delay of embryo development by handling in vitro. 5. The vitrification procedure is considered to be a very simple and efficient method for cryopreservation of embryos at early developmental stage.

• Journal of Embryo Transfer
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• v.17 no.2
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• pp.117-121
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• 2002

This study was carried out to investigate the in vitro fertilization rate of canine immature oocytes cryopreserved by vitrification freezing. The vitrification solutions of EPS and EDS were consisted of 40％ ethylene glycol 18％ Ficoll and 0.3M sucrose, and 20％ ethylene glycol, 16.5％ DMSO and 0.5M sucrose in TCM-199 medium supplemented with 10％ FCS, respectively. The oocytes were exposed The developmental rate of in vitro cultured oocytes recovered from ovaries collected at different stages of the reproductive cycle were 3.8％, 10.7％, 46％, respectively. to EFS or EDS at $25^{\circ}C$ and loaded into straw fer 30 sec. The straws was slowly immersed into L$N_2$. Fertilization and survival rate was defined as development rate on in vitro culture or FDA-test. 1. The fertilization rate after vitrification freezing of immature oocytes at 1, 6, 12 and 24 hrs after collection from ovaries was very low(5.3％～31.4％) than the unfrozen oocyte(60.0％). And the fertilization rate after vitrification freezing of immature oocytes was very higher than that of mature oocytes. 2. The survival rate after vitrification freezing of immature oocytes at 1, 6, 12 and 24 hrs after collection from ovaries was 55.0％, 40.0％, 28.6％ and 17.1％, respectively. And the survival rate after vitrification freezing of immature oocytes was slightly higher than that of mature oocytes.

• Journal of the Korean Data and Information Science Society
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• v.23 no.1
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• pp.1-11
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• 2012

When the microarray experiment developed, main interest was limited to detect differentially expressed genes associated with a phenotype of interest. However, as human diseases are thought to occur through the interactions of multiple genes within a same functional category, the unit of analysis of the microarray experiment expanded to the set of genes. For the phenotype of censored survival time, Gene Set Enrichment Analysis(GSEA), Global test and Wald type test are widely used. In this paper, we modified the Wald type test by adopting normal score transformation of gene expression values and developed a parametric test which requires much less computation than others. The proposed method is compared with other methods using a real data set of ovarian cancer and a simulation data set.

• Korean Journal of Food Science and Technology
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• v.35 no.2
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• pp.302-305
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• 2003

Effect of feeding far infrared-irradiated rice on survival ability of mice against drowning was investigated. Moisture absorption of far infrared-irradiated rice was about 1.3-fold higher than that of normal rice. L-Value was maintained high during cooking and storage. Weight of mice was not influenced by far infrared-irradiated rice content. No significant difference in survival abilities of mice between normal rice and far infrared-irradiated rice was shown until 1 breeding week. Significant differences in survival ability between two groups were found in 2 (9.5%) and 3 breeding weeks (7.2%).