Rhodotorula glutinis was inoculated in the medium containing 0 ($S_0$), 0.01 ($S_1$), 0.1 ($S_2$) and 1.0% ($S_3$) Ganodoma lucidum extract and incubated in a shaking incubator at $30^{\circ}C$ for 8 days and the cell growth, sugarity, lipid content, and fatty acid composition were measured to investigate the effects of G. lucidum extract on the growth and biosynthesis of fatty acids by oleaginous yeast, Rhodotorula glutinis. After the 8 day incubation, the cell growth of $S_1,\;S_2\;and\;S_3$ increased 1.6, 1.7 and 2.1 times, respectively, than that of $S_0$. Sugar consumption of incubating medium was decreased but the crude lipid content in R. glutinis was increased with increase of the amount of G. Lucidum extract. Myristic, palmitic, stearic, oleic and linoleic acids were identified by GC as the major fatty acids in the crude lipid produced by R. glutinis and the content of unsaturated fatty acids (38.6 mg/g) was greater than that of saturated fatty acids (22.3 mg/g). As the G. lucidum extract concentration increased, fatty acids contents were increased except myristic acid, and the most increase occurred at the addition of 0.1% while they were considerably decreased in the case of the addition of 1.0% G. lucidum extract.
This study was conducted to establish the proper techniques of the recirculating wick hydroponics for safe seed tuber proliferation of potatoes (Solanum tuberosum L. CV. Dejima). To achieve these, several intact tubers (5, 10 and 20 g) and cut seed-pieces (two or four) were treated in wick hydroponic system beds. A polystyrene box (31cm in width, 20cm in height, 51cm in length, and $0.031m^3$ in volume) was placed on a styrofoam hox. Eight wicks $(width\;1.5cm\;{\times}\;length\;40cm) $ were put into each polystyrene hox and the boxes were filled with perlite + peatmoss (1 : 2, v/v) medium. Top fresh weight per plant increased with increasing the tuber size from 10 to 30g/tuber. As the tuber size increases from 10 to 30 g/tuher in case of uncut tuber, the marketable tuber (>5g) production per plant increased from 83.8 to 141.8 g/plant and the marketable tuber (>5g) production per plant of cut tuber was slightly higher than that of uncut treatment. Total tuber yield ranged from 4.16 to $6.56kg/m^2$ and the percentage of seed tubers was greater than $97\%$ for all treatments. These results indicate that seed tuber should be cut to produce large tuber $(\geq10g)$ in the recirculating wick hydroponic system.
This study was carried out to investigate the effect of germination condition and drying temperature on growth and physicochemical properties of brown rice. Three brown rice seeds of Ilpumbyeo, Dasanbyeo and Heugjinjubyeo were stored at room temperature for six weeks to test the time-sequence germination viability. Relatively stable germination ratio was maintained until 2 weeks after storage. However, 3 weeks after storage, germination ratio of brown rice seeds started to decrease rapidly and their germination ratio was lower than 80%. For this reason, brown rice was recommended for seeding within 2 weeks after hulling. During the initial 5 days, germination ratio of 24 hours pre-soaking brown rice was higher about 2-3% than that of non-soaking brown rice. The $25^{\circ}C$ was considered as the most favorable temperature for brown rice germination, because of the high germination ratio and desirable coleoptile growth of the brown rice, and little seed rotting symptoms. The scanning electron micrographs showed the structural differences between hot-air dried and freeze dried germinated-brown rice kernel. In the freeze dried germinated-brown rice, seed coat (pericarp, tegmen and aleurone layer) was mechanically disrupted from the endosperm, and many cleavages were observed among starch storing cells and starch granules. The endosperm of freeze-dried brown rice kernels formed the sponge-like structures and showed the fragile traits. For this reason, hot-air drying is considered as more suitable method than freeze drying for germinated-brown rice. The crude protein and amylose contents were slightly changed, but there were no significant differences during the germination period. Crude fiber content was decreased, but crude Int and total amino acid contents were increased as seeding days increased. A rapid increase in $\alpha$-amylase activities of germinating brown rice was observed at S days after seeding, and $\alpha$-amylase activities were decreased from 8 days after seeding. Total free sugar contents were decreased during the germination period. There was continuous decline in the contents of sucrose and glucose until 8 days after seeding, but fructose and maltose content were gradually increased from the 5 days after seeding.
A large amount of oyster-shell waste has been illegally disposed at oyster farm sites along the southern coast of Korea, which already created serious environmental problems. Therefore, the study was undertaken to increase the consumption of oyster shell meal as a soil amendment. The effects of oyster shell meal on dynamics of heavy metals and uptake of heavy metals by spring Chinese cabbage were evaluated in silt loam soil (in Gyeongsang National University, Jinju, Gyeongnam-do, Korea), where 0, 4, 8, 12 and 16 Mg $ha^{-1}$ oyster-shell meal fertilizer were added. Lime treatment (2 Mg $ha^{-1}$) was selected as a control. In the results of this study, cabbage yields were increased by increasing levels of oyster-shell meal fertilizer. With increasing levels of oyster-shell meal fertilizer, total heavy metals concentrations were not significant among treatments. However, 0.1N HCl extractable heavy metals concentration was significantly reduced due to increasing of soil pH. A lot of portion (ca. $80{\sim}90%$) heavy metals fraction of all fractions was residual phase in soil after harvesting. The contents of Cu, Mo, Zn in cabbage were slightly increased by increasing levels of oyster shell meal fertilizer. However, there were no toxic symptoms of heavy metals during cultivation. Conclusively, it was estimated that oyster shell fertilizer could be a good amendment to increase productivity of crop and reduce uptake of heavy metals by crop and mobility of heavy metals in soil.
This study was conducted to find out the effect of slow-release nitrogen fertilizer, such as latex coated urea(LCU) and Meister10(MS10) on direct seeded rice in dry soil(DS). Junghwabyeo, and early maturity rice cultivar was grown on the plots which were treated with None-nitrogen. urea. LCU and MS10 plot. Growth characteristic, yield and yield components were investigated. Nitrogen uptake-efficiency and physico-chemical properties of soil before-after experiment were analyzed. Plant height and number of tillers $m^{-2}$ in LCU and MS10 plot at early grow stages were higher than those in urea plot. Plant height and number of tillers $m^{-2}$ grown on the plot of Ms10 plot were higher than those of LCU plot. The number of seedling $m^{-2}$ were no significant differences among None-N, urea, and MS10 plot in DS. Heading date and leaf color were higher with Urea than LCU and MS10 plot. Culm length in LCU and MS10 plot were longer compared with urea plot, but panicle length was similar among with Urea, LCU and MS10 plot. Number of panicles $m^{-2}$ was greater in order of MS10 > LCU > Urea plot. Yield were greater in order of MS10 > LCU > Urea plot. Nitrogen uptake and nitrogen efficiency were greater in order of MS10 > LCU > urea plot. After the experiment, total content of nitrogen in soil was not changed at all treatments, but pH, P and Si of soil were lower than those of before experiment at all treatments.
This study was conducted to find out the effect of slow-release nitrogen fertilizer, such as latex coated urea(LCU) and Meister10(MS10) on direct seeded rice in flooded soil(FS). Junghwabyeo, and early maturity rice cultivar grown on the plots which were treated with None-nitrogen, urea, LCU and MS10 plot. Growth characteristic, yield and yield components were investigated. Nitrogen uptake-efficiency and physico-chemical properties of soil before-after experiment were analyzed. Plant height and number of tillers $m^{-2}$ in LCU and MS10 plot at early grow stages were higher than those of Urea plot. MS10 and LCU plot showed similar tendency on the plant height but MS10 plot was higher than LCU plot on the number of tillers $m^{-2}$. The number of seedling $m^{-2}$ were no significant differences among None-N, urea, and MS10 plot. Heading date and leaf color were no significant differences among None-N, urea, and MS10 plot. Culm length in LCU and Ms10 plot were longer compared with urea plot, but panicle length were similar among with Urea, LCU and MS10 plot. Number of panicles $m^{-2}$ was highest in MS10 plot and it was similar between LCU and Urea plot. Yield were greater in order of Ms10 > LCU > Urea plot. Nitrogen uptake and nitrogen efficiency were greater in order of MS10 > LCU > urea plot. After the experiment, total content of nitrogen in soil was not changed at all treatments, but pH, P and K of soil were lower than that of before experiment at all treatments.
PARK, JUNG-IM;KIM, JAE HOON;KIM, JONG-HYEOB;KIM, MYUNG SOOK
The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
/
v.24
no.4
/
pp.548-561
/
2019
The surfgrass Phyllospadix japonicus is a dominant seagrass species playing critical ecological roles on the eastern coast of Korea. However, few studies have been conducted on the ecological characteristics of this species, generally due to the turbulent water conditions in its habitat. In this study, to examine the growth dynamics of P. japonicus, we investigated monthly changes in morphological characteristics, density, biomass, and leaf productivity as well as changes in the underwater irradiance, water temperature, and water column nutrient concentrations of its habitat from August 2017 to July 2018. Underwater irradiance and water temperature showed clear seasonal changes increasing in spring and summer and decreasing in fall and winter. Nutrient availability fluctuated substantially, but did not display any distinct seasonal trend. Morphological characteristics, shoot density, biomass, and leaf productivities of P. japonicus exhibited significant seasonal variations, increasing in spring and decreasing in fall months. Spadix of P. japonicus occurred from March to August, with the maximum spadix percentage(15.8%) occurred in May 2018. The average leaf productivity of P. japonicus per shoot and area were 2.1 mg sht-1 d-1 and 7.5 g m-2 d-1, respectively. The optimum water temperature for the growth of P. japonicus in this study was between 13-14℃. The productivity of P. japonicus was not correlated with underwater irradiance, water temperature and nutrient concentrations. These results suggest that the study site provide sufficient amount of underwater irradiance, suitable water temperature range and nutrients for the growth of P. japonicus.
A study was conducted to evaluate the weed population as affected by repeated application of fertilizers for 15 years (nonfertilized, PK, NK, NPK, NPK+Compost, NPK+Straw and NPK+lime). Alopecurus aequalis authority did not grow at all without P application and lime reduced the population of A. aequalis. Total number of weeds were the largest at 5-10cm soil layer and increased by application of compost and straw. Monochoria vaginalis dominated in NPK+compost and NP plot. Scirpus hotarui dominated in NPK+straw. Both Eleocharis kuroguwai and Potamogeton distinctus dominated in nonfertilized plot. Numbers of M. vaginalis and S. hotarui were larger low pH 6 while that of E. kuroguwai and P. distinctus at pH 6 to pH 7. Incidence of M. vaginalis and S. hotorui was great at pH's lower than 6, while E. kuroguwai and P. distinctus favored pH's ranging from 6 to 7.
Bog bean (Menyanthes trifoliata L.) is an endangered species in Korea, inhabiting in montane wetlands. Its' habitats are decreasing due to artificial disturbances and climate change in Korea. Interspecies competitions determine the distribution of a species and it is important to examine the effects of companion species on the growth of a species. To reveal the effects of habitat substrate and companion species on the growth of bog bean, we compared the growth parameters of bog bean at three substrate conditions where plant communities were formed: water column, Sphagnum mat, and paddy soil. Bog bean had the highest relative coverage at Sphagnum mat condition and there are few species whose height were higher than it. In contrast, there were many species larger than bog bean at paddy soil condition. The maximum coverage of bog bean was the largest at water column condition, and the lowest at paddy soil condition. Our results indicate that the growth of bog bean was more restricted by interspecific competition at paddy soil condition than at Sphagnum mat condition. Those results suggest that bog bean avoid competitions by expansion of rhizome rather than elongation of its height. For conservation or creation of bog bean habitats, it is necessary to offer the environment where other species cannot survive well.
RAPD test and the observation of morphological, cultural characteristics of fourteen selected entomogenous fungi were conducted to investigate the analysis of their internal relationships. Paecilomyces tenuipes showed snow flower form attached to numerous white conidiophores, produced globular and semi-egg types on the club types of phialides. Cordyceps militaris formed globosely conidiophores, dark yellow fruiting body on pupa. The phialide as on Acremonium-type in global conidiophores. Beauveria bassiana covered with conidia was not formed fruiting body and adhered conidia on conidiophore of zigzag type. The PDA and SDAY medium were confirmed as an optimum growth of them. P. tenuipes showed to velvet and plane types in several media whereas C. militaris was belong to centrally raised and floccose in the morphological type. In contrast, B. bassiana covered with conidia on velvet shape. The size of amplified products were analyzed by RAPD using URP primer and were from 100 bp to 2.0 kb with $10{\sim}14$ geuomic DNA. Total similarities of two groups were by dendrogram of UPGMA analysis. The homology of P. tenuipes groups was 94.8 to 100%. It also showed 70.1 to 96.6% in C. militaris group and B. bassiana was higher similarity than any other. The internal change of C. militaris, produced telemorph fruiting body, was higher seperated in species than P. tenuipes and B. bassiana in the RAPD.
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