• 제목/요약/키워드: 생약제

검색결과 402건 처리시간 0.023초

Hypoglycemic Effect of Paeonia lactiflora in High Fat Diet-Induced Type 2 Diabetic Mouse Model (고지방식이 유발 제2형 당뇨모델 마우스에서 작약의 혈당강하 효능)

  • Yoon, In-Soo;Jung, Yujung;Kim, Hyun Jung;Lim, Hyun Jin;Cho, Seung-Sik;Shim, Jung-Hyun;Kang, Bok Yun;Cheon, Seung Hoon;Kim, Su-Nam;Yoon, Goo
    • Korean Journal of Pharmacognosy
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    • 제45권3호
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    • pp.194-199
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    • 2014
  • The roots of Paeonia lactiflora (PL) has been traditionally used as analgesic, spasmolytic and tonic in Korea, China, and Japan. As part of a search for herbal medicine to treat diabetes and obesity, we confirmed hypoglycemic effect of PL through high fat diet-induced obese and diabetic mice experiments in vivo. Treatment of ethanolic extract from PL led to a significant decrease in glucose level, which is comparable to that of an antidiabetic drug metformin. In addition, PL selectively stimulates the transcriptional activities of both peroxisome proliferator-activated receptor $(PPAR){\alpha}$ and ${\gamma}$, and inhibits enzymatic activity of protein tyrosine phosphatase 1B (PTP1B), which are predicted to be therapeutic target in treatment of type2 diabetes and obesity. Especially, the n-hexane fraction (Hx) from PL ethanol extract showed more potent activities on $PPAR{\alpha}$ and than others and exihibited moderate inhibitory activity against PTP1B.

Cytotoxic Effect of Korean Traditional Prescriptions on the Human Gastric Cancer Cell Lines (한약처방제의 인체 위암 세포주에 대한 세포독성 효과에 관한 연구)

  • Kim, Eun-Hae;Eun, Young-Ah;Kang, Bong-Joo;Sung, Hyun-Jae;Park, Kap-Joo
    • Korean Journal of Pharmacognosy
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    • 제28권4호
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    • pp.233-238
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    • 1997
  • ln order to search for antigastric cancer agents from Korean traditional prescriptions. We selected 41 traditional prescriptions, based on a review of the Korean traditional medicine books. Both boiling water and methanol extracts were tested, by means of the Sulforhodamine B (SRB) protein assay. Six of the 41 water extracts; #3, #34, #35, #38, #40, #41 showed efficacy against gastric cancer cell (AGS: Human gastric carcinoma, ATCC HTB 103). #3 inhibited 50% cancer cell growth1 at the concentration of $152\;{\mu}g/ml$, #34, #35, #38, #40 and #41 inhibited 50% cancer cell growth at the concentration of $145\;{\mu}g/ml$, $129\;{\mu}g/ml$, $173\;{\mu}g/ml$, $10\;{\mu}g/ml$ and $19\;{\mu}g/ml$ respectively. Ten of the 41 methanol extracts; #1, #3, #32, #33, #35, #36, #37, #38, #41 were active. #1 inhibited 50% cancer cell growth at the concentration of $206\;{\mu}g/ml$, #3, #32, #33, #35, #36, #37, 738, #40, #41 inhibited 50% cancer cell growth at the concentration of $133\;{\mu}g/ml$, $159\;{\mu}g/ml$, $199\;{\mu}g/ml$, $147\;{\mu}g/ml$, $113\;{\mu}g/ml$, $187\;{\mu}g/ml$, $130\;{\mu}g/ml$, $9\;{\mu}g/ml$, $15\;{\mu}g/ml$ respectively. Prescription #3, #35, #38, #40, #41 were also interesting because both methanol and water extracts were active.

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Studies on the Processing of Crude Drugs(IX) -Preparing Standardization and Regulation of Stir-Frying Glycyrrhzia root(1)- (한약수치에 관한 연구(제 9보) -초감초(炒甘草) 제법의 표준화 및 규격화(1)-)

  • Choi, Hyuck-Jai;Lee, Woo-Jung;Park, Sung-Hwan;Song, Bo-Whan;Kim, Dong-Hyun;Kim, Nam-Jae
    • Korean Journal of Pharmacognosy
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    • 제36권3호통권142호
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    • pp.209-219
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    • 2005
  • In this study, we carried out the preparing standardization and regulation of processed Glycyrrhizae Radix (PGR) which have been widely used in oriental medicines. Glycyrrhizae Radix(GR) have been generally prepared by the stir-frying, or mix-frying with honey for the purpose of decreasing sweetness and augmenting vitality. Firstly, we tried to standardize PGR prepared by the stir-frying. We purchased 14 kinds of PGR and non-processed GR(NPGR) at oriental physician's offices and oriental pharmacies on a nation scale, respectively. The amounts of dry on loss, water extract, diluted ethanol extract, ether extract, total ash, acid insoluble ash, glycyrrhizin(GL), glycyrrhetic acid(GA) and liquiritin(LQ) of them were examined. The amounts of dry on loss, GL and LQ in commercial PGRs showed remarkable decrease, while GA showed increased as compared with NPGR. In order to standardize preparing method of PGR, the effect of heating time on physico-chemical parameters and biological activities were examined. Physico-chemical parameters such as dry on loss, extract amount, GL and LQ contents in PGRs showed decrease, however, GA was increased with heating time as compared with NPGR. Also, GA, obtained from heat-treated GR, was found as an artifact in PGRs. PGR was more effective than NPGA in vitro test of DPPH scavenging effect and TBA-Rs reducing effect. PGR and NPGR showed potent hepatoprotective effect on $CCl_4-intoxicated$ rats. Especially, PGR prepared by 80 min of heating was the most effective. Considering these results, the optimal condition for PGR preparation was $150^{\circ}C$ for 80 min.

Analysis of Alkaloids in Uncaria Hooks (조구등 중의 알칼로이드 분석)

  • Park, Man Ki;Park, Jeong Hill;Kim, Jong Moon;Han, Sang Bum;Han, Bang Hee;Kang, Jong Seong
    • Analytical Science and Technology
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    • 제6권4호
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    • pp.395-399
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    • 1993
  • Corynoxeine, rhynchophylline, isocorynoxeine, isorhychophylline were isolated from Uncaria hooks and determined by HPLC. Their contents were : isocorynoxeine 0.21%, isorhynchophylline 0.16%, corynoxeine 0.28%, rhynchophylline 0.23%, respectively. Corynoxeine and isocorynoxeine, rhynchophylline and isorhychophylline were interconverted each other by heat treatment.

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Preparation of Antiinflammatory Herbal Drug, SKI306X. (항염작용을 갖는 신규 생약복합제 SK1306X의 분리 및 항염작용)

  • 박광식;김환수;안재석;김택수;박병욱;곽의종;한창균;조용백;김기협
    • YAKHAK HOEJI
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    • 제39권4호
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    • pp.385-394
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    • 1995
  • Antiinflammatory activities of the solvent fractionates of several herbal medicines were investigated and SKI306X was prepared from the active principles of three herbal medicines, Prunella vulgaris, Trichosantlies kirilowii and Clematis mandshurica. SK1306X was shown to have strong inhibitory effects on acetic acid-induced pain, carrageenan-induced paw edema and adjuvant induced arthritis. LD50 of SKI306X was more than 5 g/kg in rat, so generally nontoxic. Chemical analysis revealed that oleanolic acid and rutin, which are known to have various antiinflammatory activities, were contained in it. These results suggest SK1306-X may become a useful drug for the treatment of inflammatory diseases such as rheumatoid arthritis.

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L1210 및 K562 세포에 대한 노루발의 세포독성 성분

  • 배기환;이상명
    • Proceedings of the Korean Society of Applied Pharmacology
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    • 한국응용약물학회 1994년도 춘계학술대회 and 제3회 신약개발 연구발표회
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    • pp.246-246
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    • 1994
  • 연구목적: 생약으로 부터 항암제 개발연구의 일환으로 민간에서 조경. 지혈 등에 사용되고있는 노루발(Pyrola japonica)의 세포독성 물질과 그의 화학적구조를 규명하기 위해서 본 연구를 실시하였다. 실험 방법 : 옴건한 노루발의 지하부를 methanol로 환류 추출하여 얻은 methanol extract를 n-hexane, ethylacetate, n-butanol 순으로 분획하였다. 그 후 각각의 분획을 L1210 세포에 대하여 in vitro에서 실험한 결과 n-heane, ethylacetate 분획에서 강한 활성이 나타남을 알 수 있었다. 따라서 n-hexane, ethylacetate 분획을 반복하여 silica gel column chromatography 하여 1종의 활성물질과 4종의 비활성물질을 단리하였다. 실험결과 : 단리된 활성물질은 각종 spectral data를 검토하여 chimaphilin (L1210의 ED$_{50}$ : 0.6 $\mu\textrm{g}$/ml, K562의 ED$_{50}$ :< 0.6 $\mu\textrm{g}$/ml) 임을 확인하였고 이 물질은 5,8-dihydro-2.7-dimethyl-4-naphtoquione의 변성물질 임을 알 수 있었다.있었다.

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Identification of Ginger Components and Quantitative Determination of 6-Gingerol from Crude Drugs-Drink Preparations (생약복방 드링크제 중 건강성분의 확인 및 6-Gingerol의 분리 정량)

  • 고성룡;최강주
    • Journal of Ginseng Research
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    • 제14권3호
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    • pp.442-446
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    • 1990
  • As a part of studies on the quality control of crude drug-drink preparations, ginger components were identified by TLC and 6-gingerol was determined quantitatively by HPLC. Ginger components were identified by TLC with benzene/acetone (4:1, v/v, on silica gel plate by spraying a vanillinsulfuric acid reagent. 5-Gingerol contents were determined at 280 nm by HPLC on Lichro CART RP-18 column with acetonitrile/wate(38:62, v/v). Its transfer rate in the 3 types of crude drug extract drinks was 65.4-85.1% compared to the content in the ginger extract.

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Sudies on the Antimutagenicity of Ginseng Extracts 1. The mutagenicity of ginseng and medicinal herb extracts. (인삼추출성분의 항변이원성 연구 (제1보 인삼등 생약식물 추출액의 변리원성))

  • Jeong, Ho-Gwon;Jo, Chang-Suk;Kim, Jeong-Hyo
    • Journal of Ginseng Research
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    • 제8권1호
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    • pp.1-7
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    • 1984
  • In order to confirm the antimutagenicity of ginseng extracts, mutagenicity of ginseng and several medicinal herbs of which extracts were used as drinks, was examined through the method of Ames test. The obtained results were as follows. 1. Strong mutagenicities for Salmonella tyhimurium TA98, TA100, TA1535 were observed in all sample herbs Paenonia aalbiflora, Rehmannia glutinosa, Astragalus membranaceus, Angelica acutiloba, Cnidium officinale, Laurus nobilis and Panax ginseng without S-9 mix metabolic activation. 2. In the case of S-9 mix metabolic activation, even in a low concentraction, the extracts of Angelica autilobu, Cnidium officinale and Paenonla albtilora showed also a high mutagenicities for the strain TA98 and TA1535. 3. Even in high concentration of ginseng extracts, mutagenicity was almost neglectable through the metabolic activation of S-9 mix, compared with other extracts of medicinal herbs.

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Development of Antitoxic Agents from Korean Medicinal Plants (Part 3) Repaired Effects of Methanol Fraction of Perilla Frutescens on 3T3 Fibroblast Treated with Cadmium (한국산 생약으로부터 해독물질의 개발(제3보) 카드뮴으로 처리한 3T3섬유모세포에서 소엽 메탄올 분획의 수복효과)

  • 한두석;유현경;곽정숙;백승화
    • Toxicological Research
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    • 제11권2호
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    • pp.229-234
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    • 1995
  • This study was carried out to evaluate the cytotoxicity of cadmium on 3T3 fibroblast and to develop the antidote on 3T3 fibroblast which was injuried by $IC_{50}$ of cadmium. The groups for repaired effects were divided into 7 groups such as medium alone treated group. Cadmium treated $IC_{50}$ groups and 5 experimental groups $(IC_{50}$ cadmium plus $10^{-4}$ concentration of each methanol fraction). After incubation for 48 hrs in the same conditions, MTT (tetrazolium MTT), NR (neutral red) and SRB (sulforhodamine B protein) assay were measured. Light microscopic observations were also investigated. The ethyl acetate fraction of Perilla frutescens showed significantly repaired effect against cadmium cytotoxiclty and this fraction inhibited critical cell regeneration in light microscopy.

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Development of Antitoxic Agents from Korean Medicinal Plants. Part 4. Effects of Panax ginseng C. A. Meyer Extract on the Accumulation of Cadmium in Liver (한국산 생약으로 부터 해독물질의 개발(제4보) 흰쥐 간장내의 카드뮴 축적에 미치는 인삼 추출물의 영향)

  • 백승화;유일수;이종섭;한두석
    • Toxicological Research
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    • 제11권2호
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    • pp.235-239
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    • 1995
  • This study was conducted to investigate the metallothionein (MT) induction by Panax ginseng C. A. Meyer in cadmium chloride intoxication. The results were as follows: Generally, detoxicatlon effects by Panax ginseng C. A. Meyer extract increased proportionally to the increase of cadmium concentrations. When a 8 mg/g dosage of cadmium was administered, formation of the cadmium and EDTA complex showed the highest antitoxic effect. Also, when a 4 mg/g dosage of cadmium was administered, Panax ginseng C. A. Meyer extract showed the highest antitoxic effect in metallothionein induction. According to the above results, Panax ginseng C. A. Meyer extract administered with cadmium increased metallothionein concentration and decreased the toxicity of cadmium in liver.

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