• Journal of Digital Convergence
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• v.11 no.7
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• pp.237-242
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• 2013

When abdomen and pelvic were scanned with 128 channel MDCT, the gonadal exposure dose was measured with and without gonadal shield and the obtained images were evaluated. As a result, during abdominal MDCT scan, the gonadal exposure dose was measured $16.5{\pm}0.5$ mGy when the gonad shield was not used, and it was $7.5{\pm}0.3$ mGy when the large gonad shield($650m^2$) was used, which showed the effect of reduction in the gonadal exposure dose by 54%. During pelvic MDCT scan, the gonadal exposure dose was $9.5{\pm}0.3$ mGy when the gonad shield was not used, and it was $2.8{\pm}0.2$ mGy when the large gonard shield($650m^2$) was used, which showed the effect of reduction in the gonadal exposure dose by 70%. The images were obtained when using the gonad shield and when not using it during MDCT scan, and as a result of analyzing them with 5-point Likert scale, in the abdominal image, it was 4.1 points irrespective of whether using the gonad shield or not. And also, in pelvic scan, it was 1.2 points when the gonad shield was used, and 4.1 points when it was not used. With the results above, it is considered that during the abdominal 128-MDCT scan, by using the gonad shield, the images should be obtained without being degraded and the exposure dose must be reduced.

• Proceedings of the Korean Magnestics Society Conference
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• 2015.11a
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• pp.58-58
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• 2015

• Proceedings of the Korea Society of Poultry Science Conference
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• 2001.11a
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• pp.71-73
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• 2001

In this study, we could improve transmission efficiency of germline chimeras by transfer of gonadal PGCs (gPGCs) cultured in vitro. Of hatched recipient chicks, 301 chickens (141 males and 160 females) were brought up to sexual maturity and these WLs (KOC) were mated with KOCs for testcross, resulting in 27 germline chimeras (15 males and 12 females) identified by black feather color of their progenies. The production efficiency of germline chimera production of experimental groups was observed (P=0.6831). The average transmission efficiency of proven germline chimeras was 0.6 ∼56.5% (15.0% on average). The transmission efficiency of experimental group which were transferred 10-days cultured gPGCs without Ficoll treatment was highest (49.7%) and that of experimental stock which transferred non-cultured gPGCs with Ficoll treatment was lowest (0.6%). The duration of in vitro culture before transferring was significantly important for the high efficiency of germline transmission. Transferring 10-days cultured gPGCs made the transmission efficiency higher rather than transferring non-cultured and 5-days cultured gPGCs, 50 times and 10 times, respectively (p<0.0001). However, Ficoll treatment for increasing the population ratio of gPGCs negatively affected the transmission efficiency and the effects of sexuality and the reciprocal interaction between treatments showed no significant differences. These findings demonstrated that the crucial factors for improving the germline transmission were the duration of in vitro culture prior to transfer. Thus, we developed the complete system for production of germline chimera using cultured gPGCs with highly improved efficiency and this system would be useful for genetic manipulation and obtaining the transgenic aves.

• Journal of the Korean Society of Radiology
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• v.13 no.6
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• pp.889-894
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• 2019

Upper gastrointestinal series is an examination that uses X-rays. It is important to defend against exposure to radiation during upper gastrointestinal examination because the organs, such as thyroid gland, lens, breasts, and gonads, with relatively high biological sensitivity to radiation are distributed on the examination area. We have made a whole body phantom that can change the depth of organs. radiation dose of eye, thyroid gland, breast and gonads were measured by the same procedure as the actual upper gastrointestinal examination. When performed only fluoroscopy the mean dose reduction of lens, thyroid gland, breast and gonads was 62.2%. The mean dose reduction of lens, thyroid gland, breast and gonads was 59.0% when both fluoroscopy and spot shoot were performed. Therefore, when performed upper gastrointestinal examination it was confirmed that shielding of the lens, thyroid gland, breast and gonads was effective in decreasing the exposure dose. The manufactured human phantom can be used in measuring radiation dose for deep organ because it can adjust the height corresponding to the organs located in the human body.

• 대한방사선방어학회:학술대회논문집
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• 2011.04a
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• pp.8-9
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• 2011

• Proceedings of the Korea Society of Poultry Science Conference
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• 2003.11a
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• pp.71-72
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• 2003

최근 생쥐에서 정원세포를 이용한 생식선 카이메라의 생산이 보고되었다. 정원세포의 경우 성축으로부터 세포를 다량으로 얻기가 쉬우며, 수용체 정소 내로 이식될 경우 생식선 카이메라의 생산능력이 있어서 이전의 배아줄기 세포를 이용할 때의 문제점을 효율적으로 해결할 수 있다. 또한 유전자가 도입된 정원세포의 이식에 의한 수용체 정소 내에서의 정자형성의 보고는 정원세포를 이용한 형질전환 동물의 생산 시스템으로의 개발 가능성을 보여준다. 본 실험에서는 닭에서 기존에 이용되어 왔던 형질전환 동물 생산 시스템의 문제점을 극복하고자 주령별 정원세포의 분리 및 이식을 통하여 조류에서 정원세포의 이식방법을 확립하고 생식선 카이메라 생산효율을 증진시키기 위하여 불임제인 부설판 등을 이용한 불임화 기술을 확립하여, 결국 조류에서의 형질전환 조류 생산 시스템으로서의 개발가능성을 제시하고자 한다.

• Development and Reproduction
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• v.11 no.3
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• pp.219-226
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• 2007

This study was conducted to identify optimistic primordial germ cells'(PGCs) migration activity using heat activated busulfan treatment for the increasing germline chimerism. Donar PGCs viability tests of important conditions for useful germ line chimerism indicated approximately $70{\sim}80%$ viability was time dependent. Transplantation experiments of PGCs into recipient embryos after busulfun treatment, showed the treatment group having 23.5% viability. By comparison, the control group showed 4.8% viability. The 96 hour treatment group and the 118 hour treatment group of the cultured PGCs showed high migration activity. Generally, the transplantation method would consider morphological and physiological characteristics before transplantation. In the present study, the effect of busulfan on migration activity showed viability highest at 53.4% after 48-hour incubation time. However, a previous study showed the best condition for transplantation time to be prior to the 48-hour incubation period, when the chicken embryo does not yet have a developed blood vessel system. In conclusion, an important condition for the production of a transgenic chicken is that most donor PGCs migrate into the recipient embryo without any inhibitory factors. The present results suggest, perhaps by using this modified method of transplantation, it can produce a more efficient chimeric germ line, transgenic chicken.

• Journal of Radiation Protection and Research
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• v.36 no.1
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• pp.23-27
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• 2011

In order to confirm feasibility of MOSFET modality in use of in.vivo dosimetry, evaluation of gonad shielding in order to minimize gonadal dose of patients undergoing radiotherapy by using MOSFET modality was performed. Gonadal dose of patients undergoing radiotherapy for rectal cancer in the department of radiation oncology of Seoul National University Hospital since 2009 was measured. 6 MV and 15 MV photon beams emitted from Varian 21EX LINAC were used for radiotherapy. In order to minimize exposed dose caused by the scattered ray not only from collimator of LINAC but also from treatment region inside radiation field, we used box.shaped lead shielding material. The shielding material was made of the lead block and consists of $7.5\; cm\;{\times}\;9.5\;cm\;{\times}5.5\;cm$ sized case and $9\;cm\;{\times}\;9.5\;cm\;{\times}\;1\;cm$ sized cover. Dosimetry for evaluation of gonad shielding was done with MOSFET modality. By protecting with gonad shielding material, average gonadal dose of patients was decreased by 23.07% compared with reference dose outside of the shielding material. Average delivered gonadal dose inside the shielding material was 0.01 Gy. By the result of MOSFET dosimetry, we verified that gonadal dose was decreased by using gonad shielding material. In compare with TLD dosimetry, we could measure the exposed dose easily and precisely with MOSFET modality.

• Proceedings of the Korean Aquaculture Society Conference
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• 2003.10a
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• pp.21-21
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• 2003

경골어류의 뇌하수체에서는 두 종류의 생식선자극호르몬 (FSH, LH)이 생산되며, 이 호르몬들은 공통적인 α 쇄와 특이적인 β 쇄를 가진다. 연어과 어종들에서, FSH는 난황형성과 정자형성의 역할을 하며, LH는 배우자의 최종성숙을 조절한다. 냉수성 고유어종인 열목어 (Brachymystax lenok)의 멸종을 방지하고 종묘생산을 원활히 하기 위하여 먼저 열목어의 GTHα, FSHβ 및 LHβ 쇄를 cloning하여 염기서열을 결정하였다. 열목어 GTHα, FSHβ 및 LHβ의 cDNA는 산천어의 해당 cDNA와 높은 상동성 (각각 84, 95, 98%)을 보였다. 다음으로 기능적인 생식선자극호르몬을 제작하기 위해서 2개의 쇄를 single-strand로 연결하여 진핵세포를 숙주로하는 시스템에서 생식선자극호르몬을 생산할 수 있는 구조체인 FSHβ-GTHα (235 amino acids) 와 LHβ-GTHα (240 amino acids)를 각각 재조합하였다. 또한 각각의 융합단백질 생산용 구조체의 3'-말단에는 단백질추출이 용이하도록 histidine×6 구조를 첨가하였다. 이상의 단일쇄 FSH와 LH 유전자재조합 산물을 포유동물 유래의 세포 (CHO-K1)에 liposome chemical을 사용하여 유전자도입 후 세포에서 분비되는 단백질을 모니터링하였다. 배지를 부분정제한 후 SDS-PACE로 조사한 결과, LH 재조합 유전자를 도입한 후 48-60 시간째에 약 25 kDa의 단백질로서 관찰되었다. 현재 FSH 재조합 유전자에 대해서도 조사중이며, 향후 이를 재료로 하여 기능형 생식선자극호르몬을 생산하고 추출하기 위한 연구가 계속적으로 수행 될 것이다.

• Journal of the korean veterinary medical association
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• v.24 no.8
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• pp.465-472
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• 1988