• Korean Journal of Microbiology
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• v.41 no.1
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• pp.24-37
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• 2005

This study was conducted to analyze the molecular epidemiological properties and to select the most efficient and reliable PCR method on 116 of Staphylococcus aureus (S. aureus) isolates from Korean cattle, black goat, pig, dog, chicken, mouse and also human clinical cases from hospital. The distribution patterns of SSG [species specific genes; coagulase (coa), protein A (spa), nuclease (nuc) and aroA (RsaI) gene] were analyzed by PCR method. Among the SSGs, the nuc-gene was found in all strains $(100\%)$ tested and followed by coa-gene $(87.9\%)$, spa-gene $(91.4\%)$ and aroA-gene $(26.7\%)$, in order. The genetic subtyping by RFLP method was performed on the coa [AluI] and aroA-gene [RsaI] PCR products. The mecA-gene PCR and PCR-RFLP techniques were chosen to detect and verify of MRSA strains. Only the human strains $(12.1\%)$ were detected the positive mecA-gene products (533 bp), which were divided into two specific bands [201 & 332 bp] by HhaI enzyme digestion. On coa-gene and spa-gene typing, coa-gene was typed with ten kinds of genotype and coa-3 type were determined as the most predominant genotype, while spa-gene was divided into eleven kinds of genotype and also spa-7 type were selected the most prevalent genotype based on their genetic variations. On the aroA and coa-gene subtyping by PCR-RFLP, aroA-gene products were discriminated with only seven types of genotype, while coa-gene products were further divided into an eleven genotype, respectively. In comparison of SID values of five PCR based typing methods, the coa-PCR-RFLP (SID0.894) was evaluated the most efficient and reliable tools and followed by coa-PCR (SID0.883) and aroA-PCR-RFLP (SID0.462), in order. In conclusion, we could determined that the coa-PCR-RFLP method was the most suitable genetic analysis tool for S. aureus and MRSA strains from domestic animals and humans.

• Korean Journal of Organic Agriculture
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• v.26 no.1
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• pp.99-128
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• 2018

The cultivation area of rice as staple grains is decreasing in the domestic situation in Korea. Import volume of a duty in foreign rice is 409,000 tons for a year regardless increasing of production per unit area and decreasing of rice consumption. The total stock of rice is increasing cumulatively despite the effort for production mediation of rice. Therefore, maintenance of cultivation area and reduction of production are necessary for national foodstuffs security problems. Development of environment-friendly and low-carbon technology as alternative of global warming and aging of farm labor power is very important responsibility for descendants with creation of sustainable agriculture environment. As alternative for demand and supply stabilization of rice from all angles, first stage: extension of environment-friendly cultivation area as 17% Jeollanam-do level with maintenance of cultivation area under the present circumstances, second stage: extension of environment-friendly cultivation area as 25%, third stage: extension of environment-friendly cultivation area as 35%. From above mentioned scenario, reduction of rice production (60,000 tons), increases of production cost (59,200,000,000 Won), and reduction of income (201,500,000,000 Won) are estimated in first stage. Reduction of rice production (90,000 tons), increases of production cost (122,100,000,000 Won), and reduction of income (313,700,000,000 Won) are estimated in second stage. Reduction of rice production (380,000 tons), increases of production cost (222,000,000,000 Won), and reduction of income (464,500,000,000 Won) are estimated in third stage. From analysis results for partial tillage in transplanting cultivation complex (10ha), rice production is decreased 1.3~1.5 ton by complex. Production cost of rice is decreased and increases of income cultivation type. Gradual extension of environment-friendly agriculture and low-carbon partial tillage could be expected for environment maintenance of the territorial integrity, confidence of consumer, and high-efficiency of low-cost.

• Journal of Forest and Environmental Science
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• v.9 no.1
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• pp.44-56
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• 1993

The situation and the problems of the management of public forest owend by Kangweon provincial and Taebaek municipal govenments were investigated throughout literature and field survey. The results were as follows; 1. Kangweon provincial forest is 25,896ha and Taebaek municipal forest is 550ha, which consists of Kangweon provincial forest, 470ha and municipal forest, 80ha. Taebaek city is responsible for the management of the province-owned forest in its administrative district in addition to its own forest. 2. Except San 1-2Changjookdong (443ha) which is leased by Dai Han Coal. Coporation for mining coal, the remaining forest (107ha) consists of 32 scattered, small-area forest lands. 3. Due to small area and dispersal of municipal forest, the management base for forest production is not sound and the management at present is nearly absent. Based on these actual conditions, the following suggestions are provided for the rationalization for the municipal forest management. First, scattered, small-scale forests should be gathered to prepare a management complex. Second, the sustaining production of timber should be attained through the estblishment of systematic management plan. Third, the reasonable system for forest land utiliztion shoule be established in consideration of the recreational use of public forest.

• Journal of Food Hygiene and Safety
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• v.30 no.1
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• pp.51-58
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• 2015

Thirty one of Staphylococcus aureus isolated from perilla leaf cultivation areas in Miryang were investigated on the characteristics, such as enterotoxin genes and antibiotic susceptibility. Five toxin genes (sea, seb, sec, sed, and see) were examined by PCR method. Disc diffusion method was used to examine the antibiotic susceptibility of S. aureus by using 18 types of antibiotic discs with different concentrations. Among enterotoxin-encoding genes, sea and sed genes were co-detected from 4 isolates (12.9%), sed gene was founded in 9 isolates (29.0%), and see gene was founded in 1 isolate (3.2%). However seb and sec and tsst were not detected in any isolates. As a result of antibiotic susceptibility test, 7 isolates (22.6%) were resistant to 12 antibiotics (penicillin, ampicillin, oxacillin, amoxicillin-clavulanic acid, cefazolin, cephalothin, imipenem, gentamicin, tetracycline, ofloxacin, norfloxacin, and erythromycin). 2 isolates (6.5%) were resistant to 5 antibiotics (penicillin, ampicillin, amoxicillin-clavulanic acid, gentamycin, and telithromycin). MRSA (Methicilline Resistant Staphylococcus aureus) was founded in packing vinyl, hands, and perilla leaves.

• The Korean Journal of Pesticide Science
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• v.20 no.3
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• pp.221-227
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• 2016

This study was conducted to investigate residual organochlorine pesticides in green house soil and green perilla leaves. Extraction and clean-up method were developed using the modified QuEChERS method for residual organochlorine pesticides (ROCPs) in soil and green perilla leaves. Recovery and limit of quantitation (LOQ) of ROCPs in greenhouse soil and green perilla leaves were 76.3-113.4 and 79.4-107.3%, 0.03-0.24 and $0.33-0.50{\mu}g/kg$, respectively. Detected ROCPs in greenhouse soil were dieldrin and endosulfan sulfate, the residue were 1.6-9.2 and $22.0-87.8{\mu}g/kg$, respectively. But two pesticides in all green perilla leaf samples were not detected. These results showed that ROCPs residue in greenhouse soil was lower than the level of bioaccumulation occurring.

• Cartoon and Animation Studies
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• s.23
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• pp.1-17
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• 2011

The paper analyzed the metaphoric expressions appeared in whose cinematic quality has been subjectively recognized on the global stage by winning the Oscar for Best Animated Short Film. Many contents and visual effects of the film were expressed with metaphor. Story and sequence were analyzed through the language system-based 'Conceptual Metaphor Analysis Framework'. In addition, characters have been analyzed through the 'Visual Metaphor Analysis Framework'. The framework is to analyze visual dimensions which are derived when concepts are structuredin animation. This paper has attempted to investigate and reveal patterns how animation self-organizes or how animation self-realizes through linguistic objectives. That is, it is to reveal how animation communicates with the audience from the metaphoric perspective among many conventional ones. In an animated film, metaphor is a fundamental means to connect fragmented images, produce a holistic view and therefore induce creative communication with the audience. The essential motive of metaphor which is expressed in various styles in is modern people's feeling of loss and sense of missing something. Train, bottle top, food, lost-and-found center, in-between space and lost items have significance across the movie. According to an analysis, metaphor offers simplicity and freshness as an aesthetic function. In addition, the metaphor expresses lengthy contents and various meanings implicitly, delivering an economic function. With a creative function, metaphor is a tool to generate new meanings. In other words, an animated film is a medium to inspire and move the general public as a means of communication and thinking, not just an entertainment for young generations. After all, metaphoric expressions have been used to deliver diverse and deep meanings in animated films.

• Weed & Turfgrass Science
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• v.7 no.3
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• pp.231-238
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• 2018

This study was carried out to develope new zoysiagrass cultivars 'JangsungChorok' (Application No. :2013-3) and 'JangsungSaetbyeol' (Application No. :2013-4). One hundred and one genetic resources were collected at Jangsung Gun area from 2010 to 2011. Among the collected lines, CY6097 (JangsungChorok) and CY6069 (JangsungSaetbyeol) showed superior performance in spring green up and growth rate. 'JangsungChorok' showed genetically light green leaf color, with medium leaf width (4.4 mm), height from ground to first leaf blade of 3.2 cm and average plant height of 18 cm. Fast recovery rate and early spring green up rate will make this cultivar (JangsungChorok) for efficient sod producer. 'JangsungSaetbyeol' showed genetically dark green leaf color, with fine to medium leaf width of 3.5 mm, height from ground to first leaf blade of 4.3 cm and average plant height of 22.6 cm. Fast recovery rate and early spring green up rate with relatively narrow leaf will make this cultivar (JangsungSaetbyeol) for high sod quality.

• Journal of the Korean Geographical Society
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• v.49 no.6
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• pp.833-848
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• 2014

The assessment of the current and future climate change-induced potential wind energy is an important issue in the planning and operations of wind farm. Here, the authors analyze spatiotemporal characteristics and variabilities of wind energy over Korean Peninsula in the near future (2006-2040) using Representative Concentration Pathway(RCP) scenarios data. In this study, National Institute of Meteorological Research (NIMR) regional climate model HadGEM3-RA based RCP 2.6 and 8.5 scenarios are analyzed. The comparison between ERA-interim and HadGEM3-RA during the period of 1981-2005 indicates that the historical simulation of HadGEM3-RA slightly overestimates (underestimates) the wind energy over the land (ocean). It also shows that interannual and intraseasonal variability of hindcast data is generally larger than those of reanalysis data. The investigation of RCP scenarios based future wind energy presents that future wind energy density will increase over the land and decrease over the ocean. The increase in the wind energy and its variability is particularly significant over the mountains and coastal areas, such as Jeju island in future global warming. More detailed analysis presents that the changes in synoptic conditions over East Asia in future decades can influence on the predicted wind energy abovementioned. It is also suggested that the uncertainty of the predicted future wind energy may increase because of the increase of interannual and intra-annual variability. In conclusion, our results can be used as a background data for devising a plan to develop and operate wind farm over the Korean Peninsula.

• Korean Journal of Organic Agriculture
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• v.20 no.4
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• pp.669-685
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• 2012

This study was carried out to investigate the nutrient value and yield response of corn, barley, and sudangrass by the application of anaerobic liquid fertilizer in two tidal land soil in order to design the resource recycling agricultural system between livestock and forage crop cultivation in a environment friendly agricultural region. Forage crop yields at reclaimed tidal soil during were at the level of 64~76% comparing with forage crop yields at upland soil in the cropping system of corn, barley, and sudangrass. And yields of forage crops were in the order of barley > corn > sudangrass in the cultivated cropping system for 2 year. Relative feed values (RFVs) of forage crops were 88%~106% for corn, 90%~111% for barley, and 91%~113% for sudangrass, and the carrying capacity of beef cattle (Hanwoo) was 5.8~8.6 head/year/ha for the corn-barley cropping system of one year, and 4.8~6.7 head/year/ha for the barley-sudangrass cropping system of one year. Also, carrying capacity of beef cattle (Hanwoo) was highest in the application level of anaerobic liquid fertilizer 200%.

• Journal of The Korean Society of Grassland and Forage Science
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• v.22 no.2
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• pp.85-92
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• 2002

This study was conducted to investigate the effects of application levels of swine waste and chemical fertilizer on productivity of corn and nitrogen and phosphorus leaching in Lysimeter containing sandy loam soil. Main Plots were consisted of two types of swine waste, such as swine slurry(SS) and compost of swine manure fermented with sawdust(CSMFS), and chemical fertilizer(CF) and the subplots were consisted of application levels of N-fertilezer, such as 100kgN/ha, 200kgN/ha and 400kgN/ha. Lysimeters were constructed with 1m depth, 30cm diameter. The results obtained were summarized as follows ; 1. Dry matter(DM) yield of com enhanced as increased application levels of SS, CSMFS and CF. DM yield reveals that there is an increase in order CS > SS > CSMFS. 2. Nitrogen contents of the whole corn enhanced as increased application levels of SS, CSMFS and CF. Nitrogen content reveals that there is an increase in order CS > SS > CSMFS. 3. $NO_3^{-}$ and P contents in the leaching water increased as increased application levels of SS, CSMFS and CF. $NO_3^{-}$ content was highly elevated in the concentrated rainy season in the experimental early stage. However, P content was affected with the rainfall. The maximum $NO_3^{-}$ and P contents in the leaching water were 14.8ppm and 0.26ppm, respectively.ively.