• Korean Journal of Microbiology
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• v.44 no.1
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• pp.49-55
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• 2008

White Spot Syndrome Virus (WSSV) is one of the most virulent viral agents in the penaeid shrimp culture industry. In this study, WSSV in a Fenneropenaeus chinensis shrimp farm and an adjacent seawater were concentrated using a membrane filtration and quantified using the quantitative real-time PCR (QRT-PCR) method with newly designed primers and Taqman probe. Sensitivity of primers and probe was proven by WSSV standard curve assay in QRT-PCR. In order to demonstrate the relationship between WSSV and environmental parameters, physicochemical and biological parameters of the farm and influent seawaters were monitored from June to September, 2007. The abundance of WSSV ranged 3,814-121,546 copies per 1 liter of seawater, which was correlated with fecal enterococci ($r^2=0.9$, p=0.02), chlorophyll ${\alpha}$ ($r^2=0.8$, p=0.03) and $BOD_5$ ($r^2=0.8$, p=0.07). Subsequently, it is concluded that the QRT-PCR method using Taqman probe established in this study was efficient to clarify the quantification of WSSV in seawaters. Statistical analyses of environmental parameters obtained in this study also showed that the abundance of WSSV was correlated with several biological parameters rather than physicochemical parameters.

• Journal of Environmental Science International
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• v.28 no.6
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• pp.571-579
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• 2019

In this study, monthly average values of BOD, COD, and TOC observed for 10 years (2008-2017) in the Nam River were estimated, and monthly variations of BOD, COD, and TOC were analyzed. Monthly average COD was always higher than monthly average BOD; monthly average TOC was high from June to September when rainfall was high. Monthly correlation coefficients between BOD and COD ranged from 0.57 to 0.94, those between BOD and TOC from 0.45 to 0.93, and those between COD and TOC from 0.75 to 0.93. The correlation coefficients were high from November to February when rainfall was low. Regression analyses for monthly average water quality data of the Nam River classified into dry season (October to April) and wet season (May to September) were conducted. Correlation coefficients were higher in the dry season than those in the wet season, and the determination coefficients of linear regression functions for BOD and COD with TOC were also higher in the dry season than those in the wet season. From this study, it can be concluded that it is appropriate to use monthly data to analyze the correlations among BOD, COD, and TOC in the stream. To analyze the relationship between TOC flowing into the stream and BOD/COD, it was found that seasonal characteristics should be considered.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.24 no.3
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• pp.185-192
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• 1991

The vertical distribution and chemical characteristics of water masses were measured along two south-north transects in the polar front region of the central Korean East Sea. In February, a thermocline was present at depth between 50m and loom at the southern sites of a landward A-transect, and its depth was gradually deepened northward. At an outside B-transect, a thermocline was observed at significantly deep depth of 300m to 400m at two northern stations(Stn. 10 and 11), though the depth of the southward stations was nearly identical to that at the northward stations on a A-transect. In September, there were vertically more various water masses, i.e. the Tsushima Warm surface water(TWSW) or more than $20^{\circ}C$, the Tsushima Middle water(TMW) with a range of $12{\~}17^{\circ}C$, the North Korea Cold Water(NKCW) with $1{\~}7^{\circ}C$ temperature, the Japan Sea Proper Water(JSPW) of less than $1^{\circ}C$, and the mixed water. The North Korea Cold Water could be distinguishable from the other waters, especially from the mixed water of the Tsushima Middle Water and the Japan Sea Proper Water by the pattern of $T-O_2$ diagram. For instance, the North Korea Cold Water had higher oxygen by $1{\~}2ml/l$ than those in the mixed water, although both the two water masses ranged $1{\~}7^{\circ}C$ in water temperature. AOU value was the highest in the JSPW and the lowest in the TWSW. Also, AOU indicated a nearly linear and negative correlation with water temperature. However, AOU data for two masses, the NKCW and the TMW, in September departed remarkably from a regression line. Moreover, the ratio of $$\Delta P/\Delta AOU)$ in September was about $0.45{\mu}g-at/ml$ and higher than the value observed in the open sea. This high value could be elucidated by two factors; intrusion of the NKCW with high oxygen and molecular diffusion of dissolved oxygen from the surface into the lower layer. AOU would be a useful tracer for water masses in the polar front area of the Korean East Sea.

• Journal of the Korean Society for Marine Environment & Energy
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• v.7 no.1
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• pp.13-21
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• 2004

Temporal changes of Chl-α, physical and chemical factors were investigated by diurnal observation at 2-hour interval at three fixed stations in the western Chinhae Bay from 12 Aug. to 13 Aug. 1999. Difference of dissolved oxygen between surface and bottom layer was maximum when the thermocline were strong. Organic distribution such as COD was affected by the growth of phytoplankton. Limitting factor was nitrogen, that is, inorganic nitrogen plays a significant role on regulating the algal growth. Surface distribution of dissolved inorganic nitrogen was very low compared to bottom layer by uptake of organisms. Maximum value of Chl-α at station C2 and C11 were observed from subsurface layer, ranges of which exceeded possibility concentration of red tide outbreak, 10 mg/㎥. On the other hand, that of C15 exist at surface layer. In this area, DIN and DIP concentrations increased by input sources such as rainfall and benthic flux before the bloom of phytoplankton. Accumulation of phytoplankton occurred at subsurface layer by the rapid uptake of DIN, especially nitrate ion, when strong thermocline existed as approach to the afternoon, which led to the increase of organics in water column and oxygen deficiency water mass at bottom layer until late at evening. Since then, DIN increases gradually as water temperature decrease to minimum. The quantitative understanding of nitrogen of fluxed to and from the various sources is necessary for environmental management.

• Economic and Environmental Geology
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• v.35 no.1
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• pp.43-54
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• 2002

The Chonju and Samchun streams are palling though Chonju City and several contamination sources are located along these streams. The Samchun stream joins the Chonju stream in the Gosapyeong waste disposal site and the Chonju stream finally joint to the Mankyeong River. The Chonju and Samchun streams are now contaminated with BOD, COD, T-N and T-P and the amounts of each contamination are increasing from upper stream to downstream. At the downstream the amounts of COD. T-N.T-P are several times higher than fifth grade of water quality thor lacustrine. Sewage from Chonju provides BOD, COD,T-N and T-P into the Chonju and Samchun streams and Chonju Waste Water Treatment Plant il a main source of COD, T-N and T-P contamination. Gosapyeong waste disposal site may be the source of BOD and COD contamination. T-N is higher than fifth grade of water quality for lacustrine at the upper stream indicationg that the rivers are contaminated with T-N before inflowing into Chonju by agricultural activity. The Chonju stream asffects the contamination of the Mankyeong river with BOD. COD, T-N,T-P. Other branch steams of the Mankyeong river also affects the contamination of the Mankyeong river by BOD, COD, T-N and T-P, Among the branch streams, the Ikasn stream is a main contamination source. Amounts of concentrations inflowing from the Chonju and Sanchun Streams on Aug. 1999 are calculated by using yeasured flow rate find concentrations of contaminants The result are as fikkiws; 1) the amounts of influent from the Gosan Stream are 0.49, 0.86, 1.61 and 0.01 ton/day for BOD, COD, T-N and T-P, respectively, 2) compared to the amounts of influent from the Gosan Stream, BOD, COD,T-N,T-P supplied from the Chonju river are higher by about 5, 7, 7. 36 times, respectively, and those supplied from the Iksan stream are higher by about 13, 10, 10, 147 times, respectively.

• KSBB Journal
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• v.23 no.1
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• pp.90-95
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• 2008

In this study, a combined process of sequential anaerobic-aerobic digestion (SAAD), fluidized-bed bioreactor (FBBR), and ultrafiltration (UF) for the treatment of small scale food waste leachate was developed and evaluated. The SAAD process was tested for performance and stability by subjecting leachate from food waste to a two-phase anaerobic digestion. The main process used FBBR composed of aerators for oxygen supply and fluidization, three 5 ton reaction chambers containing an aerobic mesophilic microorganism immobilized in PE (polyethylene), and a sedimentation chamber. The HRTs (hydraulic retention time) of the combined SAAD-FBBR-UF process were 30, 7, and 1 day, and the operation temperature was set to the optimal one for microbial growth. The pilot process maintained its performance even when the CODcr of input leachate fluctuated largely. During the operation, average CODcr, TKN, TP, and salt of the effluent were 1,207mg/L, 100mg/L, 50 mg/L, and 0.01 %, which corresponded to the removal efficiencies of 99.4%, 98.6%, 89.6%, and 98.5%, respectively. These results show that the developed process is able to manage high concentration leachate from food waste and remove CODcr, TKN, TP, and salt effectively.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.35 no.1
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• pp.21-26
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• 2002

The behavior of the biological water treatment process on start-up operation was evaluated in the integrated recirculating aquaculture system consisting of a double drain type rearing tank ($2.5 m^3$), a sedimentation tank, a floating bead filter, a foam separator and a rotating biological contactor. A system was stocked with nile tilapia (Oreochromis niloticus) at an initial rearing density of $2\%$ for 2 weeks for acclimated rotating biological contactor. The total ammonia nitrogen (TAN) level increased to $13.6 g/m^3$ on day 4 after adding feed and was decreased to $0.3 g/m^3$ on day 7. The total suspended solid was completely removed during overall experimental period.

• Korean Journal of Ecology and Environment
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• v.39 no.2 s.116
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• pp.145-156
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• 2006

The objective of this study was to evaluate biological health conditions and physicochemical status using multi-metric models at five sites of the Keum-Ho River during August 2004 and June 2005. The research approach was based on a qualitative habitat evaluation index (QHEI), index of biological integrity (IBI) using fish assemblage, and long-term chemical data (1995 ${\sim}$ 2004), which was obtained from the Ministry of Environment, Korea. For the biological health assessments, regional model of the IBI in Korea (An,2003), was applied for this study. Mean IBI in the river was 30 and varied from 23 to 48 depending on the sampling sites. The river health was judged to be "fair condition", according to the stream health criteria of US EPA (1993) and Barbour et al. (1999). According to the analysis of the chemical water quality data of the river, BOD, COD, conductivity, TP, TN, and TSS largely varied epending on the sampling sites, seasons and years. Variabilities of some parameters including BOD, COD, TP, TN, and conductivity were greater in the downstream than in the upstream reach. This phenomenon was evident in the dilution by the rain during the monsoon. This indicates that precipitation is a very important factor of the chemical variations of water quality. Community analyses showed that species diversity index was highest (H=0.78) in the site 1, while community dominance index was highest in the site 3, where Opsariichthys uncirostris largely dominated. In contrast, the proportions of omnivore and tolerant species were greater in the downstream reach, than in the upstream reach. Overall, this study suggests that some sites in the downstream reach may need to restore the aquatic ecosystem for better biological health.

• Journal of Aquaculture
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• v.16 no.1
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• pp.15-23
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• 2003

We have investigated water quality of the coastal saline groundwaters utilized for fish farms in Jeju Island. The water quality investigation included the spatial observations for 75 fish farms during March-May, 1994 and the hi-monthly observations for both coastal saline groundwaters and seawaters at four fish farms from August 1994 to December 1995. Water temperature of the saline groundwaters ranged from 16 to 18$^{\circ}C$ over the study period. Salinity of the saline groundwaters varied between 20.60 ppt and 34.02 ppt, slightly lower than that of the coastal seawaters(26.47~34.53 ppt). This salinity variation must be associated with local precipitation conditions in Jeju Island. The oxygen saturation for most saline groundwater samples was lower than 80%, ranging from 24.7 to 89.8%. The COD and pH values for the saline groundwaters were similar to those for the coastal seawaters. The concentrations of DIP for the saline groundwaters varied between 0.021 mg/L and 0.121 mg/ L, and seasonal variation of DIP in the saline groundwater ranged from 0.014 to 0.077 mg/L, which were higher than that of the coastal seawaters(0.000~0.015 mg/L). Nitrate in the saline groundwaters accounted for more than 90% of the DIM. The maximum concentrations of ammonia, nitrite, nitrate and DIN in the saline groundwaters were 0.085, 0.012, 2.294 and 2.309 mg/L, respectively. These concentrations of the saline groundwaters were considerably lower than those affected culture organisms. Overall, the saline groundwaters utilized for fish farms in Jeju Island appear to maintain good waterquality for fish farms.

• Journal of Navigation and Port Research
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• v.40 no.6
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• pp.441-450
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• 2016

A pilot scale biostimulation experiment was performed under field conditions to degrade organic pollutants and PAHs (Polycyclic Aromatic Compounds) in contaminated coastal sediment. During the experiment, the seawater temperature around the sediment varied from $16.5^{\circ}C$ to $21^{\circ}C$, while the sediment pH was stable at 8.4-8.5. The experiment was conducted over a one year period with a control group and a sample group with a applied biostimulant composed of acetate, sulfate, and nitrate. Chemical oxygen demand decreased 39% in the control group and 79% in the sample group. Volatile solids were reduced from 15 to 7 g/kg in the control group and 2.5 g/kg in the sample group. Out of the 2-, 3- ,4- ,5-, and 6-rings of the 16 vital PAHs, the compound including naphthalene (2-ring), took 2 months to degrade completely, while the degradation efficiency was 55.6% in the control group during the same period. In the case of the 3-ring and 4-ring PAHs, complete degradation in the sample and 46% - 100% degradation in control was observed after one year of the experiment. The 5-ring and 6-ring PAHs were degraded to about 77%-100% in the sample group and 26%-87% in the control group during the one year of the experiment. The study results show that biostimulation is a very effective method to improve the degradation rate of organic pollutants and PAHs (Polycyclic Aromatic Compounds) in contaminated coastal sediment.