• Title/Summary/Keyword: 생물학적방제

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Biocontrol of Anthracnose of Chili Pepper by Bacillus sp. NAAS-1 (Bacillus sp. NAAS-1을 이용한 고추 탄저병 생물학적 방제)

  • Yoo, Jae Hong;Park, In Cheol;Kim, Wan Gyu
    • The Korean Journal of Mycology
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    • v.40 no.4
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    • pp.277-281
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    • 2012
  • Bacillus sp. NAAS-1 isolated from the field of Chili pepper was tested for biocontrol activity against anthracnose pathogen of Chili pepper caused by Colletotrichum acutatum. The antifungal activity of Bacillus sp. NAAS-1 culture broth was compared with synthetic fungicide containing carbendazim (40%) and kasugamycin (3.45%). Bacillus sp. NAAS-1 showed a similar fungicidal activity against the anthracnose pathogen at the concentration of 50 ${\mu}L/mL$ in comparison to the fungicide containing carbendazim (40%) and kasugamycin (3.45%) using a cup method. Bacillus sp. NAAS-1 also exhibited its potent fungicidal activity against the anthracnose in vivo test at the concentration of 50 ${\mu}L/mL$ when compared to the fungicide containing carbendazim (40%) and kasugamycin (3.45%).

Biological control of grapevine crown gall (포도나무 줄기 혹병의 생물학적 방제)

  • Chung, Kwang-Jin;Shim, Jae-Seop;Chung, Bong-Koo
    • The Korean Journal of Pesticide Science
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    • v.2 no.2
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    • pp.97-101
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    • 1998
  • Agrobacterium vitis causes a crown gall disease in grapevine and that is one of the major hindrances for the wide cultivation and production of grapevine. We studied the possibility of biological control using selected biological control agent. One isolate from the infected soil, named as strain 27, was able to inhibit the biovar 1; A. tumefaciens C58 and Ach5, biovar 2; A. rhizogenes 13264, and biovar 3; A. vitis, in vitro and in vivo test. The putative biological control agent, A. radiobacter strain 27 was carrying the plasmid and the size of isolated plasmid was very similar to that of pAgK84 of A. radiobacter K84.

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작은구슬산호말 (Corallina pilulifera)의 Methanol 추출물로부터 항적조물질 분리

  • ;Long-Guo JIN
    • Proceedings of the Korean Society of Fisheries Technology Conference
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    • 2001.05a
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    • pp.209-210
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    • 2001
  • 적조에 대한 대책으로서 가장 많이 이용되는 황토법은 적조생물을 침전시키므로써 제거하지만, 황토의 침전에 의한 2차적인 오염의 가능성이 있다 (Na et al., 1996). 황산구리, 과산화수소와 Triosyn에 의한 적조 살조효과가 있지만 이러한 화학물질은 모든 생물에 대한 살조효과가 있으므로 현상에 사용하기는 힘들다. 생물학적 방제에는 바이러스, 박테리아의 방법이 강구되고 있지만, 산소고갈 등의 2차적인 부작용이 야기된다. (중략)

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Conservation condition of academic museum cultural properties and biological environmental investigation (대학박물관 수장환경조사 및 생물학적 환경조사)

  • 이중수;배미현;이민수;박지선;황석연;김기현
    • KOMUNHWA
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    • no.64
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    • pp.111-131
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    • 2004
  • Academic museum have to prevent damage about a microorganisms(bacteria, fungi) and damage in current facilities and environmental condition, and an exhibition or garnered relics and are so, analysis checks maintenance environmental condition of an academi

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Biological Control of Malaria Vector (Anopheles sinensis Wied.) by Combined Use of Larvivorous Fish (Aplocheilus latipes) and Herbivorous Hybrid (Tilapia mossambicus niloticus) and Herbivorous Hybrid (Tilapia mossambicus niloticus) in Rice Paddies of Korea (천적포식어 (Aplocheilus latipes)와 식식성 어류 (Tilapia mossambicus niloticus) 의 병합적 처리에 의한 논에 서식하는 말라리아 매개모기 (Anopheles sinensis Wied.) 의 생물학적 방제)

  • 유효석;이준학
    • Korean journal of applied entomology
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    • v.28 no.4
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    • pp.229-236
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    • 1989
  • A combined field release of indigenous larvivoroug fish (Aplocheilus latipes) and hybrid herbivore (Tilapia mossambicus miloticus) was conducted to determine the effectiveness of biological control, in particular, against malaria vector (anopheles sinensis), breeding in weedy habitat of rice fields at the University rice paddies at Suwon during the period of June through September in 1988. A combined fish introduction at the release rate of 2.0 fish per $M^{2}$ for Aplocheilus and 1-pair $10M^{2}$ water surface for Tilapia resuited in 70.8% Anopheles larval reducton in a week period, the rates of rontrol increased to 73. 5% and 80.2% in 4th and 5th week respectively after the fish introdurtion, maintaining mosquito suppression in range of 80~82% control after the 5th week against Anopheles and Culex spp. combined. In a single fish treatment with Aplocheilus at 2.0 $fish/M^{2}$ release rate, Anopheles larval reduction ranged on the average 51.4~56.5%in 4 wrek period which was later integratpd with B.t.i. (Rl53.78) treatment at 1.0 kg/ha dosage rate to suppress vector mosquito population down and the the results was compared with that of combined fish introdurtion.

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Development of Antagonistic Microorganism for Biological Control of Pythium Blight of Turfgrass (잔디 피시움마름병(Pythium blight)의 생물학적 방제를 위한 길항 미생물의 선발과 효력 검정)

  • Jung, Woo-Chul;Shin, Taek-Su;Do, Ki-Suk;Kim, Won-Kuk;Lee, Jae-Ho;Choi, Ki-Hyun
    • Research in Plant Disease
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    • v.12 no.3
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    • pp.260-266
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    • 2006
  • Pythium blight caused by Pythium spp. is one of major diseases in putting green of golf course. In this study, microorganisms which are anatgonistic to Pythium aphanidermatum, a pathogen of pythium blight, were selected primary through in vitro tests, dual culture method and triple layer agar diffusion method. In vivo test against pythium blight were conducted to select the best candidate biocontrol microorganism by pot experiment in a plastic house. Bacillus subtilis GB-0365 was finally selected as a biocontrol agent against pythium blight. Relative Performance Indies(RPI) was used as a criterion of selecting potential biocontrol agent. B. subtilis GB-0365 showed resistance to major synthetic agrochemicals used in golf course. Alternative application of synthetic agrochemicals and B. subtilis GB-0365 was most effective to successfully contol pythium blight. B. subtilis GB-0365 suppressed the development of pythium bight of bentgrass by 56.4% as compared to non-treated control and its disease control efficacy was 60.9% of a synthetic fungicide Oxapro(WP) efficacy. B. subtilis GB-0365 has a potential to be a biocontrol agent for control of pythium blight.

Biocontrol of Rice Diseases by Microorganisms (미생물을 활용한 친환경적인 벼 병해 방제법)

  • Kim, Jung-Ae;Song, Jeong-Sup;Jeong, Min-Hye;Park, Sook-Young;Kim, Yangseon
    • Research in Plant Disease
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    • v.27 no.4
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    • pp.129-136
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    • 2021
  • Rice is responsible for the stable crop of 3 billion people worldwide, about half of Asian depends on it, and rice is grown in more than 100 countries. Rice diseases can lead to devastating economic loss by decreasing yield production, disturbing a stable food supply and demand chain. The most commonly used method to control rice disease is chemical control. However, misuse of chemical control can cause environmental pollution, residual toxicity, and the emergence of chemical-resistant pathogens, the deterioration of soil quality, and the destruction of biodiversity. In order to control rice diseases, research on alternative biocontrol is actively pursued including microorganism-oriented biocontrol agents. Microbial agents control plant disease through competition with and antibiotic effects and parasitism against plant pathogens. Microorganisms isolated from the rice rhizosphere are studied comprehensively as biocontrol agents against rice pathogens. Bacillus sp., Pseudomonas sp., and Trichoderma sp. were reported to control rice diseases, such as blast, sheath blight, bacterial leaf blight, brown spot, and bakanae diseases. Here we reviewed the microorganisms that are studied as biocontrol agents against rice diseases.

Biological Control with Streptomyces sp. on Fusarium oxysporum f. sp. vasinfectum and Phytophthora nicotianae var. parasitica Causing Sesame Wilt and Blight (Streptomyces sp. 에 의한 참깨 시들음병 (Fusarium oxysporum f. sp. vasinfectum) 및 역병 (Phytophthora nicotianae var. parasitica)의 생물학적(生物學的) 방제(防除))

  • Chung, Bong-Koo;Hong, Ki-Sung
    • The Korean Journal of Mycology
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    • v.19 no.3
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    • pp.231-237
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    • 1991
  • This study was conducted in order to find out biological control of sesame wilt and blight caused by Fusarium of oxysporum f. sp. vasinfectum and Phytophthora nicotianae var. parasitica by using Streptomyces spp. Two sesame pathogens, Fusarium oxysporum f. sp. vasinfectum and Phytophthora nicotianae var. parasitica were purely isolated from diseased sesame plants of the field. Streptomyces species were isolated from 72 soil samples collected from red pepper and sesame uplands in Chungbuk and selected as antagonists according to the results of dual culture. The selected Streptomyces isolates such as St-11 and St-20 were confirmed their antagonistic effect through mycelial inhibition zone and inhibitory effects on the mycelial growth of the pathogens by culture filterate of the antagonists. Inhibitory effects on the conidial germination of Fusarium oxysporum vasinfectum and Phytophthora nicotianae parasitica by the antagonists were also tested in addition to mycelial Iysis. The antagonists St-11 and St-20 showed inhibitory effect on growth of sesame seedlings after seeds soaked in the suspension. Effect of soil inoculation with antagonist St-11 showed 40 to 78 percent of control effect for two diseases in comparison with control under greenhouse.

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The Effects of Controlling the Aphis gossypii Glover (Homoptera, Aphididae) on Cucumber of Entomopahtogenic Fungus, Beauveria bassiana

  • Kang, Min-Ah;Youn, Young-Nam
    • Korean Journal of Agricultural Science
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    • v.35 no.2
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    • pp.127-136
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    • 2008
  • Beauveria bassiana can be used as a biological insecticide to control a number of pests. It has been known as that B. bassiana TBI-1 have a insecticidal effect on the twospotted spider mite (Teranychus urticae) and the greenhouse whitefly (Trialeurodes vaporariorum) these days. The biological pest control agent, B. bassiana, showed somewhat insecticidal activities against the cotton aphid, Aphis gossypii, too. After treatment with biological insecticide, the mean of increasing rate of aphids was at around recorded at around 0.21 while at around 0.24 in non-treated TBI-1 biological insecticide condition during 1 week. Consequently, it was found that treatment of Beauveria bassiana reduced an increasing rate of aphid population. Also, we could visually observe dead aphids changed as a reddish mold due to this insecticidal fungus. B. bassiana TBI-1 can significantly reduce an increasing rate of aphid population, so effective biological control against the cotton aphid are possible.

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Antagonistic Effects of the Bacterium Alcaligenes sp. HC12 on Browning Disease Caused by Pseudomonas agarici (버섯 세균성회색무늬병균(Pseudomonas agarici)에 대한 Alcaligenes sp. HC12의 항균활성)

  • Lee, Chan-Jung;Moon, Ji-Won;Cheong, Jong-Chun;Kong, Won-Sik
    • The Korean Journal of Mycology
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    • v.44 no.3
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    • pp.171-175
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    • 2016
  • A gram-negative bacterium was isolated from spent substrates of Agaricus bisporus and showed significant antagonistic activity against Pseudomonas agarici. The bacterium was identified as Alcaligenes sp. based on cultural, biochemical, physiological characteristics and a 16S rRNA sequence analysis. The isolate is saprophytic, but not parasitic or pathogenic on cultivated mushroom, whereas it showed strong inhibitory effects against P. agarici cells in vitro. The control efficacy of Alcaligenes sp. HC12 against brown blotch of P. agarici was up to 63% on Agaricus bisporus. The suppressive bacterium may be useful for the development of biocontrol systems.