• Journal of the Korea Institute of Military Science and Technology
• /
• v.22 no.4
• /
• pp.575-580
• /
• 2019

In biological warfare, it is important to identify biological agents for proper treatment. We focused on developing a real-time RT-PCR kit that can detect multiple species of biological agents. AccuPower(R) Biothreat Real-Time RT-PCR Kit(v3.0) could detect Bacillus anthracis, Yersinia pestis, Vibrio cholerae, Francisella tularensis, Salmonella typhi, Rickettsia prowazekii, Variola virus, Hantaan virus, Yellow fever virus, Brucella spp., Shigella dysenteriae in a single reaction. The results showed that the kit was verified to be able to detect at least 0.005 ng of nucleotide and 10,000 CFU/ml of bacteria. Therefore, the kit is expected to be used as a rapid and sensitive detection kit for 11 species of biological agents within 2 hours.

• 동물약계
• /
• no.110
• /
• pp.3-5
• /
• 2007

동물약사감시 행정처분 결과 홍보/생물학작용제 수출입허가제도 시행/동물용의약품의 국가검정면제 및 검정수수료 등에 관한 규정 개정에 따른 간담회 참석/동물약품 마케팅 및 GMP 교육 실시/동물용의약품공정서 개정 자문위원회 참석/동물용의약품 품질관리 강화방안 추진/혁신형 기업화 맞춤지원 프로그램 시행/중국산 동물용의약품 수급관리 철저/제4차 이사회 개최/동물약품기술연구소 검사시설 준비금 각출/협회 임원 보선 결과/협회 직원 인사/VIV ASIA 2007 전시회 단체 참가

• Journal of the Korea Institute of Military Science and Technology
• /
• v.14 no.2
• /
• pp.289-298
• /
• 2011

TMAH-based Py-MS has been investigated to apply for a real-time classification of biological agents in the field. Acquiring reproducible data from mass spectrometry is a key to biological detection in the field. Nevertheless, it has been little studied on what factors could affect to the reproducibility of the TMAH-based Py-MS spectrum patterns. Given the TMAH-based Py-MS applied to the field system, several factors which could affect to the reproducible pattern of TMAH-based Py-MS spectra are needed to be examined, including changes in TMAH injection volume, growth temperature for microorganism, and number of cells collected in pyrolyzer, and implication of stabilizer used for lyophilization. This study showed that the reproducibility of the spectrum patterns was significantly hindered by changes in TMAH concentration and cell number, and stabilizer implication but not by growth temperature. Among those at low TMAH concentration(0.015m) was not observed the significant alterations of the spectrum pattern even when its injection volume was changed, yet was in different cell numbers and stabilizer implication.

• Membrane Journal
• /
• v.24 no.2
• /
• pp.167-175
• /
• 2014

For selectively permeable membrane based on cellulose acetate butyrate (CAB) and polyethyleneimine (PEI) with water vapor transportability and DMMP protective performance, we intended to improve protective performance of the membrane against CEES using several additives. Results showed that CAB/PEI membranes possessed performance with good water vapor permeation (${\geq}1,800g/m^2/day$) and enhanced protective capability against CEES contamination ($7.1{\sim}11.5{\mu}g/cm^2{\cdot}day$). Of these membranes, the membrane containing $Ag^+$ ion and ionic exchange resin showed the best protective performance. And, we identified that the CAB/PEI membranes show excellent protection against aerosols with various particle sizes ($0.005{\sim}3{\mu}m$) simulating biological agents.

• Journal of the Korea Institute of Military Science and Technology
• /
• v.19 no.5
• /
• pp.669-674
• /
• 2016

The purpose of this study is to demonstrate the suitability of hydrogen peroxide($H_2O_2$) vapor system for platform interior decontamination. Geobacillus stearothermophilus biological indicator(BI) strips and a field tent were used as a biological simulant and as a simulated platform, respectively. Decontamination was performed based on injection rates and tent sizes with exposure time 60 minutes. We standardized the conditions for the field tent decontamination : 8.0 g/min for $30m^3$($H_2O_2$ vapor concentration of 150~500 ppm, relative humidity of 50 %) and 12.0 g/min for $60m^3$($H_2O_2$ vapor concentration of 250~400 ppm, relative humidity of 55 %). Thus we suggest the system is one of the possible candidates for decontamination of platform interiors.