• Journal of the Korean Society of Environmental Restoration Technology
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• v.26 no.2
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• pp.1-11
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• 2023

By delivering effective, in-situ conservation of biodiversity, OECMs can contribute to sustaining existing biodiversity values and improving biodiversity conservation outcomes. In this study, for the reporting of OECMs required by Kunming-Montreal Global Biodiversity Framework, the site-level assessment of the Korea National Arboretum and Buffer Zones were conducted using the assessment tool of IUCN. The site-level assessment was carried out in three steps(step 1: screening, step 2 : consent for full assessment, step 3 : the full assessment). It was found that the criteria were satisfied except for the consent for full assessment, sustainability of governance and management arrangements, and the equity of governance and management. Although the governing authority, rights-holders or any other stakeholders could be identified, the step of acknowledging and agreeing to the full OECM assessment was not possible because the governance was not established. As a result of the assessment of equitable governance and management in the aspect of recognition, procedure, and distribution based on criterion(the equity of governance and management), it is judged that more specific measures are needed in the aspect of recognition. And in the aspect of procedure, there is no legal basis for participation in governance and collection of opinions, so it is judged that there is a limit to listening and reflecting the opinions of stakeholders. In the aspect of distribution, it is necessary to further confirm whether it provides direct benefits to rights-holders such as landowners in the region. And it is necessary to prepare specific criteria to assess the important biodiversity values. Therefore, in order to promote OECM reporting in the future, it is necessary to conduct detailed research on various types of governance establishment and operation plans that can establish the basis for recognizing and agreeing to OECM assessment, specific criteria and reasonable measures to judge equity, and important biodiversity value.

• Journal of Dairy Science and Biotechnology
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• v.41 no.3
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• pp.126-137
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• 2023

In this study, we isolated lactic acid bacteria from Allium wakegi and examined the usability of culture supernatants obtained from these lactic acid bacteria. The antibacterial activity of the culture supernatant obtained from the isolated lactic acid bacteria against the pathogens Escherichia and Salmonella spp. was measured. The obtained lactic acid bacteria culture medium showed significant antibacterial activity against pathogenic bacteria in a dose-dependent manner. The effects of pH and heat denaturation on the observed anti-pathogenic bacterial activity was also investigated. Adjusting the culture supernatant to pH 7 resulted in loss of all antibacterial activity against pathogenic bacteria, suggesting that the antibacterial activity of the obtained culture supernatant against pathogenic bacteria is influenced by organic acids. Assessment of the heat stability of the anti-pathogenic bacterial activity revealed that heat treatment did not diminish activity. The obtained lactic acid bacteria culture medium is thus stable against heat.

• Journal of Dairy Science and Biotechnology
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• v.41 no.4
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• pp.191-202
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• 2023

In this study, lactic acid bacteria (LAB) was isolated from blueberries. The isolated LAB were rod-shaped and gram-positive, as shown using gram staining. In addition, the identified bacteria showed high homology to Lactiplantibacillus argentoratensis. The culture supernatant was isolated from L. argentoratensis and its antibacterial activity against the pathogenic bacteria Salmonella and Escherichia was analyzed. Culture supernatants of L. argentoratensis significantly inhibited the growth of Salmonella. Enteritidis NCCP 16947, Salmonella Typhimurium NCCP 16960, and Salmonella. Thompson NCCP 11704. Interestingly, the higher the concentration of the culture supernatant, the more significant was the antibacterial activity. Additionally, the culture supernatant of L. argentoratensis showed significant antibacterial activity against Escherichia strains. To determine whether the antibacterial substance is stable to heat and pH, the LAB culture supernatant was heat-treated under 65℃ for 30 min, 75℃ for 15 min, 85℃ for 10 min, and 100℃ for 5 min. Measurement of antibacterial activity against pathogenic strains by adding 5% of heat-treated culture medium showed the same antibacterial activity as before heat treatment. However, in a test where the pH of the culture supernatant was adjusted to 7.0 from 3.73, no antibacterial activity was observed.

• KSBB Journal
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• v.15 no.4
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• pp.411-413
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• 2000

A method is described for the rapid and simple isolation of genomic DNA from 3 mL culture of Lactobacillus crispatus KLB46 The isolated DNA using this method was shown to be an excellent substrate for restriction endonclease digestion and PCR. The method is expected to be used in gentic manipulation of L. crispatus KLB46.

• Proceedings of the Korean Operations and Management Science Society Conference
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• 2002.05a
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• pp.240-247
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• 2002

복수기의 원활한 열교환에 필요한 냉각수를 공급하기 위해 순환수펌프는 해수를 끌어들이는 역할을 한다. 각종 해양생물이나 이물질 등으로 인해 순환수펌프의 작동이 멈추는 경우가 간헐적으로 발생하고 있는 데, 이렇게 되면 복수기 튜브 쪽으로 유입되는 순환수 유량은 정상상태보다 줄어들게 된다. 이를 무시하고 원전터빈을 계속 고출력으로 가동시키면 복수기 내부의 전공도가 악화되어 작동이 정지되는 상황을 맞을 수 있으므로 터빈출력을 적정한 수준으로 낮추는 작업이 필요하다. 본 연구는 순환수펌프의 작동수 및 해수온도를 고려하여 터빈의 적정출력을 산정하고 이를 도표화하여 제시하였다. 분석결과를 기존의 운전데이터와 비교검토한 결과 대체로 일치하고 있어 운전조건을 안정적으로 유지하는 데 도움이 될 전망이다.

• Journal of Environmental Science International
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• v.32 no.5
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• pp.365-374
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• 2023

Eight years (2014-2021) of climate data were collected from an automatic weather observation system installed at the foot of Mt. Geumo in Chilgok, Gyeongbuk. Using these data, we investigated local bio-climatological indices (warmth index, WI; coldness index, CI; and effective accumulated temperature, EAT) of the mountain region adjacent to the Keimyung Dongyeong forest. The study area's WI and CI were 109.3℃ and -11.3℃ per month, respectively, averaged across 8 years. These values are indicative of an evergreen broad-leaved forest in the warm temperate climate zone, suitable for cultivating sweet persimmons and figs. Additionally, EAT in Dongyeong was 2,113.7℃, averaged across 8 years, suitable for growing crops such as corn, soybean, and potato.

• Korean Journal of Psychosomatic Medicine
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• v.20 no.1
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• pp.14-17
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• 2012

There were several documents that might reflect the great concern on the education of psychosomatic medicine in medical school from the 1960s. But the hour of class and proportion of psychosomatic medicine have been quite small among the total lecture time of psychiatry. Notwithstanding the importance of biopsychosocial perspective in practice and research there have been no agreement on the goal and content of teaching psychosomatic medicine in the medical school curriculum. Consultation-liaison psychiatric activity in the hospital were currently under-developed and educational content and process were not systematic. We should have established the goal of psychosomatic education in the medical school that includes making doctor who could not only cure disease but also care the ill patients. And we should develop the curriculum that covers essential area of psychosomatic medicine and checking system to monitor the process of education. With the continuance of psychosomatic perspectives from medical school education to clinical subspecialty we can make progress in this field.

• The Korea Journal of Herbology
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• v.36 no.5
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• pp.125-133
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• 2021

Objectives : Thymus quinquecistatus var. japonica (H.Hara) is a member of the genus Thymus of perennial aromatic herb, and it's designated as a natural monument of South Korea. It has traditionally been known to have protective or therapeutic effects on various human disease including cerebrovascular and neurological disease. Recently it was suggested that essential oil extracted from thyme has anti-fungal and anti-bacterial effect. The aim of this study is to investigate anti-inflammatory effect of Thymus quinquecistatus var. japonica in Raw 264.7 macrophage cell line. Methods : The cytotoxic effects of water and 70% ethanol extracts from Thymus quinquecistatus var. japonica, was tested using MTT assay. Inhibitory effects of the extracts to nitric oxide production and mRNA expression of inflammatory cytokines were examined by RT-PCR. Also, MitoSOX-red assay and JC-1 assay were performed to determine if the extracts can inhibit mitochondrial ROS accumulation and maintain mitochondrial membrane potential. Results : In LPS-induced inflammatory response, the extracts efficiently reduced nitric oxide NO production through inhibiting mRNA expression of iNOS enzyme. In addition, expression of the proinflammatory cytokines, IL-1𝛽 and IL-6, was also down-regulated by the extract treatments. Excessive accumulation of mitochondrial ROS induced by LPS was inhibited in the extract treated cells, which finally protected mitochondrial membrane potential. Conclusions : These results showed that water and 70% ethanol extracts from Thymus quinquecistatus var. japonica have anti-inflammatory effect through down regulation of IL-1𝛽, IL-6, and iNOS, and also have antioxidative effect against mitochondrial ROS accumulation that promote inflammatory response.

• The Journal of the Korea Contents Association
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• v.21 no.3
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• pp.114-120
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• 2021

Furniture is an essential element in the area of human residence, and the necessity of system furniture suitable for the pursuit of unity in design, which clearly distinguishes the various and flexible arrangements and characteristics of space, is gradually expanding. The elderly population in Korea currently exceeds 15%, and is expected to be close to 40% by 2050. An increasing number of elderly generations are showing different behavioral patterns, such as sharing information, health care, and seeking independence from children. In order to maintain an independent daily life, a stable living environment has become essential, but as a result of a study that safety accidents caused by households increase due to a decrease in cognitive ability and physical ability, the need for a suitable household has increased. Therefore, the biological changes and characteristics of the elderly were investigated, and the concept definition, scope and limitations of system households were studied. In addition, by grasping the changes in the home appliance market and trends in the home appliance market, the design direction of system furniture required by the elderly was presented.

• The Korean Journal of Food And Nutrition
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• v.34 no.1
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• pp.36-46
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• 2021

After ethanol (BM-E and RW-E) and hot-water (BM-HW and RW-HW) extracts were fractionated from two herbal mixtures (BM and RW), their physiological activities were investigated. All extracts consisted of more than 50% of neutral sugar, with their total polyphenol levels higher than flavonoid levels. Radical scavenging activities of EtOH extracts remained significantly higher compared to that of hot-water extracts, and in particular, RW-E showed consistently higher antioxidant activity than BM-E. When anti-inflammatory activities of the extracts were evaluated by LPS-stimulated RAW 264.7 cells at 10~500 μg/mL non-cytotoxicity doses, BM-E showed significantly higher levels of TNF-α, IL-1β, IL-6, and nitric oxide inhibitory activity than those of hot-water extracts and RW-E. Murine peritoneal macrophage cells were shown to be enhanced in crude polysaccharides (BM-CP and RW-CP fractionated from BM-HW and RW-HW) compared to hot-water extracts and polysaccharide K (PSK, positive control). Especially, RW-CP exhibited higher activity than BM-CP, and component sugar analysis showed that BM-CP mainly contained galacturonic acid, glucose, arabinose, galactose, and xylose (34.5%, 33.9%, 16.1%, 7.1%, and 6.3%, respectively), whereas RW-CP showed different measurements (29.5%, 59.2%, 5.0%, 4.5%, and 0.2%). In conclusion, two herbal mixtures could contain varying sets of physiological activities dependent on different extraction and fractionation methods.