• KSBB Journal
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• v.20 no.4
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• pp.253-259
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• 2005

This study was accomplished using attached $A^2/O$ process that contains nonsurface-modified and surface-modified polyethylene media inside the Anaerobic/Anoxic, Oxic tank, respectively. We could make the hydrophobic polyethylene media have hydrophilic characteristics by radiating ion beam on the surface of the media. The objectives of this study is to investigate the removal efficiencies of the organics and nitrogen when the step feed ratio of raw wastewater into anaerobic and anoxic tank is changed. In this case, we assumed that the denitrification rate can be improved because the nitrifiers in anoxic tank can perform denitrification using RBDCOD instead of artificial carbon sources (for example, methanol, etc.). The wastewater injection rate into anaerobic/anoxic tank was set up by the ratio of 10 : 0, 9 : 1, 8 : 2, 6 : 4, and the results of BOD removal efficiency showed similar trends with $93.3\%,\;92.6\%,\;92.4\%\;and\;91.6\%$, respectively. But the BOD removal efficiency (utilization of the organics) in the anoxic tank was in the order of 9 : 1 $(84.8\%)$, 10 : 0 $(77.0\%)$, 8 : 2 $(75.3\%)$, and 6 : 4 $(61.1\%)$. The T-N removal efficiency was most high when the ratio is 9 : 1 $(67.4\%)$, and other conditions, 10 : 0, 8 : 2, 6 : 4, showed $61.3(\%),\;60.7\%,\;55.5\%$, respectively; the ratio 6 : 4 was found to be lowest T-N removal efficiency, lower than the ratio 9 : 1 by $12\%$. Though the nitrification rate of the ratio 10 : 0, 9 : 1, and 8 : 2 showed similar levels, the ratio 6 : 4 showed considerable inhibition of nitrification, ammonia was the great portion of the effluent T-N. The advantages of this process is that this process is cost-saving, and non-toxic methods than injecting the artificial carbon source.

• Journal of Life Science
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• v.21 no.10
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• pp.1473-1480
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• 2011

Perchlorate ($ClO_4^-$) is a contaminant found in surface water and soil/ground water. Microbial removal of perchlorate is the method of choice since microorganisms can reduce perchlorate into harmless end-products. Such microorganisms require an electron donor to reduce perchlorate. Conventional perchlorate-removal techniques employ heterotrophic perchlorate-reducing bacteria that use organic compounds as electron donors to reduce perchlorate. Since continuous removal of perchlorate requires a continuous supply of organic compounds, heterotrophic perchlorate removal is an expensive process. Feasibility of autotrophic perchlorate-removal using elemental sulfur granules and activated sludge was examined in this study. Granular sulfur is relatively inexpensive and activated sludge is easily available from wastewater treatment plants. Batch tests showed that activated sludge microorganisms could successfully degrade perchlorate in the presence of granular sulfur as an electron donor. Perchlorate biodegradation was confirmed by molar yield of $Cl^-$ as the perchlorate was degraded. Scanning electron microscope revealed that rod-shaped microorganisms on the surface of sulfur particles were used for the autotrophic perchlorate-removal, suggesting that sulfur particles could serve as supporting media for the formation of biofilm as well. DGGE analyses revealed that microbial profile of the inoculum (activated sludge) was different from that of the biofilm sample obtained from enrichment culture that used sulfur particles for $ClO_4^-$-degradation.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.14 no.3
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• pp.645-654
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• 1994

Photocatalytic oxidation conditions of reactant recirculation flow rate 275 mL/min, aeration rate 2 LPM and $UV+TiO_2+H_2O_2$(500 mg/L) proved to be appropriate for water including organic materials treatment. With increasing turbidity and suspended solids concentration, at turbidity 10 NTU-suspended solids concentration 29 mg/L the phenol degradation efficiency increased, which in turn decreased at turbidity 50 NTU-suspended solids concentration 170 mg/L, however no significant differences were observed, demonstrating similar results with those obtained at zero turbidity and suspended solids concentration. The degradation efficiency of phenol decreased with increasing influent phenol concentrations. The $UV+TiO_2+H_2O_2$ photocatalytic advanced oxidation process conducted is considered to be possibly applied to the drinking water treatment, and the post-treatment process of biological wastewater treatment.

• KSBB Journal
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• v.14 no.6
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• pp.651-654
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• 1999

Using recombinant human growth hormone as a model protein, we carried out unfolding by adding a denaturant such as urea, guanidine HCl, or SDS followed by refolding by dilution and dialysis. The objectives were to monitor the structural changes during in vitro refolding process and, based on the results, to develop a quantitative method of refolding progress assessment. The changes in surface hydrophobicity were measured by fluorescence tagging of 1-anilinonaphthalene-8-sulfonate(1,8-ANS) to the hydrophobic portions, and those in the secondary structure were monitored by using far UV-CD(circular dichroism) spectroscopy. Also, we used RP-HPLC to separate and quantify the folded and unfolded proteins to correlate the result with the structure analysis. Our results indicate the surface hydrophobicity are well correlated with the formations of the secondary structure, primarily ${\alpha}$-helices, as well as the disulfide bridges. We expect this monitoring technique can be applied in industrial fields as a means to quantitatively assess the progress of in-vitro refolding of recombinant proteins.

• Journal of Korean Society of Environmental Engineers
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• v.31 no.2
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• pp.109-113
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• 2009

This study was performed to investigate the characteristics of nutrient removal of municipal wastewater in membrane bioreactor system. Membrane bioreactor consists of four reactors such as the anaerobic, the stabilization, the anoxic and the submerged membrane aerobic reactor with two internal recycles. The hydraulic retention time (HRT), sludge retention time (SRT) and flux were 6.2 h, 34.1 days and 19.6 L/$m^2$/hr (LMH), respectively. The removal efficiency of $COD_{Cr}$, SS, TN and TP were 94.3%, 99.9%, 69.4%, and 74.6%, respectively. The estimated true biomass yield, specific denitrification rate (SDNR), specific nitrification rate (SNR), specific phosphorus release rate (SPRR) and specific phosphorus uptake rate (SPUR) were 0.653 kgVSS/kgBOD/d, 0.044 $mgNO_3$-N/mgVSS/d, 0.035 $mgNH_4$-N/mgVSS/d, 51.0 mgP/gVSS/d and 5.4 mgP/gVSS/d, respectively. The contents of nitrogen and phosphorus of biomass were 8.86% and 3.5% on an average.

• Journal of Korean Society of Environmental Engineers
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• v.39 no.3
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• pp.155-163
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• 2017

Membrane filtration has become more popular in drinking water treatment recently, since the filtration can control not only particulate matters but also pathogenic microorganisms such as giardia and cryptosporidium very effectively. Pilot-scale ($120m^3/d$ of treatment capacity) and test-bed ($25,000m^3/d$ of treatment capacity) microfiltration experiments were conducted to find optimum operating mode and the critical flux. Optimum operating mode of pilot-test was assessed as inflow 1.0 min, filtration 36.5 min, air backwash 0.9 min, backwash 1.0 min and outflow 1.0 min with 50 LMH ($L/min{\cdot}m3^$) of critical flux. Critical Flux was calculated to be $50L/m^2-h$ (within TMP 0.5 bar) based on the increase formula of the transmembrane pressure difference according to the change of time at Flux 20, 40, 56 and 62 LMH in pilot operation. Chemical cleaning was first acid washed twice, and alkali washing was performed secondarily, and a recovery rate of 95% was obtained in the test-bed plant. The results of operating under these appropriate conditions are as follows. Turbidity of treated water were 0.028, 0.024, 0.026 and 0.028 NTU in spring, summer, autumn and winter time, respectively. Microfiltration has superior treatment capability and performance characteristics in removing suspended solids and colloidal materials, which are the main cause of turbidity and important carrier of metal elements, and it has shown great potential in being an economically substitute to traditional processes (sand filtration).

• Korean Journal of Food Science and Technology
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• v.47 no.1
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• pp.81-86
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• 2015

Intense pulsed light (IPL), a nonthermal technology, has attracted increasing interest as a food processing technology. However, its efficacy in inactivating microorganisms has not been evaluated thoroughly. In this study, we investigated the influence of IPL treatment on the inactivation of Escherichia coli O157:H7 depending on light intensity, treatment time, and pulse number. Increased light intensity from 500 V to 1,000 V, raised the inactivation rate at room temperature. At 1000 V, the cell numbers were reduced by 7.1 log cycles within 120 s. In addition, increased pulse number or decreased distance between the light source and sample surface also led to an increase in the inactivation rate. IPL exposure caused a significant increase in the absorption at 260 nm of the suspending agent used in our experiments. This indicates that IPL-treated cells were damaged, consequently releasing intracellular materials. The growth of IPL-irradiated cells were delayed by about 5 h. The degree of damage to the cells after IPL treatment was confimed by transmission electron microscopy.

• Journal of Korean Society of Environmental Engineers
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• v.32 no.10
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• pp.949-956
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• 2010

A method for simultaneous removal of nitrogen and phosphate from sewage by elemental sulfur denitrification with membrane bioreactor was proposed, and capacity $10\;m^3$/day of pilot plant was operated for 350 days. This study was investigated to have the effect of denitrification rate and T-P removal with the addition of Alum in Sulfur denitrification Reactor (SDR). The addition of Alum and alkalinity ($NaHCO_3$) in the effluent of MBR was tried to remove simultaneous phosphate and nitrogen in SDR. Characteristics of total nitrogen (T-N) and total phosphate (T-P) removal was compared without and with the addition of Alum as a coagulant. T-N removal without and with the addition of Alum was 92.1% and 87.8%, respectively. And denitrification efficiency was 93.8% and 87.1%, respectively. T-P removal rate was increased to 75.6% in SDR by addition of Alum (2.6~4 mg/L as Al), but T-P removal rate was about 26.7% without the addition of Alum. Therefore, denitrification rate was 6.7% of reduction but T-P removal rate was increased by addition of Alum.

• Korean Journal of Microbiology
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• v.46 no.4
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• pp.352-358
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• 2010

Bacterial community composition in activated sludge wastewater treatment bioreactors were analyzed using 16S rRNA gene-based pyrosequencing for the four different wastewater treatment processes. Sequences within the orders Rhodocyclales, Burkholderiales, Sphingobacteriales, Myxococcales, Xanthomonadales, Acidobacteria group 4, Anaerolineales, Methylococcales, Nitrospirales, and Planctomycetales constituted 54-68% of total sequences retrieved in the activated sludge samples, which demonstrated that a few taxa constituted majority of the activated sludge bacterial community. The relative ratio of the order members was different for each treatment process, which was assumed to be affected by different operational and environmental conditions of each treatment process. In addition, activated sludge had very diverse bacterial species (Chao1 richness estimate: 1,374-2,902 operational taxonomic units), and the diversity was mainly originated from rare species. Particularly, the bacterial diversity was higher in membrane bioreactor than conventional treatment processes, and the long solids retention time of the operational strategy of the membrane bioreactor appeared to be appropriate for sustaining diverse slow growing bacteria. This study investigating bacterial communities in different activated sludge processes using a high-throughput pyrosequencing technology would be helpful for understanding microbial ecology in activated sludge and for improving wastewater treatment in the future.

• KSBB Journal
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• v.21 no.4
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• pp.317-323
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• 2006

This study focused on the availability of wastewater reclamation and reusing system as one of the alternatives against the global water shortage in near future, which system is composed of two treatment steps; first, wastewater is injected into upflow $A^2O$ biofilm process(anaerobic/anoxic/oxic) reactor filled with polyethylene fixed-media, and the effluent of 1st steps continuously passed through downflow nano silver sand filter. The pH of the effluent ranged from 7.39 to 8.06(average 7.84), the $COD_{Mn}$ was $8{\sim}18mg/L$(average 12.1 mg/L), and $BOD_5$ was $2.1{\sim}10.0mg/L$(average 4.9 mg/L), that met all the wastewater reclamation and reusing system criteria. Besides, the SS concentrations of the effluent which was $3{\sim}9mg/L$(average 4.95 mg/L) met the criteria(5 mg/L), showing 94.8% of average removal efficiency. The 99.1% of the average removal efficiency of the E-coliform did not met the criteria(Not detected), which indicates the needs for the following chlorine disinfection treatment with the residual chlorine concentration of above 0.2 mg/L. There are no bacteria on the sand surface coated by nano silver. The removal efficiency of T-N and T-P that could be included into the criteria in the future was 50.3% and 27.2% respectively.