• Journal of Life Science
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• v.18 no.10
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• pp.1331-1335
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• 2008

A Klebsiella pneumoniae WL-5 with the capability of decolorizing several recalcitrant dyes was isolated from activated sludge of an effluent treatment plant of a textile and dyeing industry. This strain showed a higher dye decolorization under static condition and color removal was optimal at pH 6-8 and $30-35^{\circ}C$. More than 90% of its color of Congo Red were reduced within 12 hr at $200\;{\mu}M$ dye concentration. Malachite Green, Brilliant Green and Reactive Black-5 lost over 85% of their colors at $10\;{\mu}M$ dye concentration, but the percentage decolorization of Reactive Red-120, Reactive Orange-16, and Crystal Violet were about 46%, 25%, and 13%, respectively. Decolorizations of Congo Red and triphenylmethane dyes, such as Malachite Green, Brilliant Green, and Crystal Violet were mainly due to adsorption to cells, whereas azo dyes, such as Reactive Black-5, Reactive Red-120, and Reactive Orange-16 seemed to be removed by biodegradation through unknown enzymatic processes.

• Microbiology and Biotechnology Letters
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• v.29 no.3
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• pp.127-133
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• 2001

Pseudomonas aeruginosa KLP-2 possessing antimicro- bial activity against Legionella pneumophila was isolated from cooling tower-waters. The culture filtrates of P. aeruginosa KLP-2 showed antimicrobial activity against L. pneumophila Vibro cholerae non-Ol. Bacillus cereus, Bacillus subtilis and staphylococcus aureus. The culture filtrates of P. aeruginosa KLP-2 showed the highest anti-microbial activity against. L pneumophila among micoorganisms tested in this study. The optimal conditions of temperature, pH carbon source and nitogen source to obtain maximal antimicrobial activity from culture filtrates of P. aeruginosa KLP-2 were determined to be 35$^{\circ}C$ pH 7.0 % of glycerol and 0.6% of proteose peptone respec- tively. The antimicrobial activity of culture filtrates from P. seruginosa KLP-2 was highest against L. pneumophila when cultivated with shaking for 24 h and without shaking for 4 day at 35$^{\circ}C$.

• Microbiology and Biotechnology Letters
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• v.10 no.2
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• pp.87-93
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• 1982

The cultural conditions of Mucor plumbeus FRI 0007 were investigated for the maximum production of felt and lipid. It was found that the lower the pH and the higher the incubation temperature, the higher accumulation of the felt and lipid. Shake culture rendered higher lipid accumulation and lower felt accumulation than static culture. Maximum production of felt and lipid content were 47.8 g/$\ell$ and 50.73%, respectively, when the organisms were static-cultured at a temperature of 37$^{\circ}C$ and pH of 3.5 for 25 days latroscan thinchrographic analysis showed that the higher amount of triglyceride was obtained when static-cultured at a low pH. Fatty acid composition of the microbial lipid was affected by the incubation temperature, types of nitrogen source and speed of agitation: lower degree of saturation was observed as the incubation temperature decreased and the speed of agitation increased. Fatty acids of monoglyceride and diglyceride were mainly palmitic and oleic acids and those of triglycerides were mainly palmitic, oleic acids.

• Microbiology and Biotechnology Letters
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• v.17 no.1
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• pp.29-34
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• 1989

An intracellular accumulation of cadmium by the intact cell of an extremely cadmium tolerant yeast, Hansenula anomala B-7, was investigated in the presence of Triton X-100. The uptake of cadmium by the intact cell was efficiently enhanced up to approximately 40% or more by 0.1% of Triton X-100 and Aerosol OT, respectively. The Michaelis constant, Km, done by Lineweaver-Burk plot of accumulation velocity of cadmium vs. cadmium concentration was calculated to be 0.247mM. The optimal conditions of pH and the temperature for the effective cadmium uptake were from neutrality to alkali and 4$0^{\circ}C$, respectively. The accumulation of cadmium was increased approximately 3 times under the shaking incubation, with no correlation to shaking rate. By zinc the cadmium accumulation was decreased.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.22 no.1
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• pp.3-4
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• 1996

오늘날 화장품 산업은 역동적으로 끊임없이 변화하고 있다. 화장품은 한 사회 구성원들의 다양한 욕구, 가치관 및 생할양식에 영향을 미치며 심지어는 국가의 정치적 기후나 경제적인 상태를 나타내는 지표역할을 담당하기도 한다. 즉, 화장품 과학은 예방의학, 건강과학이라는 차원에서 뿐만 아니라 미적인 차원에서 새로운 상품개발이 진행되어갈 것이다. 지금 우리는 21세기를 향하여 1990년대의 중반기에 들어섰다. 80년대는 물질 문명의 중심으로 생활의 질의 풍요로움을 추구하였으나 90년대는 소비자 우선의 풍요로움을 향수하는 시대가 되었다. 또한 여성이 사회진출, 고령화의 급속한 진전, 고 학력화, 개인의 가치관 존중 및 많은 생활패턴의 변화가 마음의 풍요로움을 구하는 사람들이 늘어나고 물질적 의존도로 부터의 탈피가 크게 강조되고 있다. 이에 마음의 풍요로움을 창조하여 연출하는 화장품산업의 역할이 고기능성에 의한 부가가치를 높이고 마음의 풍요로움을 지원하기 위한 연구개발에 중점을 두고 진행되어 가고 있다. 화장품을 여성들의 치장이라고 하는 주체적 행위에 도움을 주는 하드웨어라고 한다면 소비자의 마음은 소프트웨어로써 소비자 입장에서 만족할 수 있는 전략을 구사하는 아이디어가 있어야 하는데 그에 상응하는 대책으로 스킨케어를 들 수 있다. 스킨케어란 병든 피부를 제외한 여러 가지 유형의 피부를 화장품과 미용기기를 이용하여 그에 맞게 예방관리 하여 건강하고 아름답게 보호 유지하는 전문 분야이다. 즉, 스킨케어는 화장품 이용을 통하여 미래의 소비자들의 다양한 비용 욕구 및 소망에 효과적으로 대응할 수 있는 소프트웨어라고 할 수 있다., 또한 스킨케어는 화장품 뿐만 아니라 신체적 접촉을 통하여 안위를 제공하는 수단으로 단순한 피부손질이라는 문제에 그치지 않고 접촉을 통해 마음과 신체의 균형을 꾀하는 생명현상의 심층과 연관되는 중요한 의미가 있다. 스킨케어는 불어로 Soin Esthetique 라고 하는데 어원을 보면 손질, 처치, 보살핌 그리고 배려를 의미한다. 지금으로부터 200년전 독일의 미학자인 Baumgarten이 미는 인간에게 만족과 쾌감을 주는 대상이며 미는 보는 사람의 마음에 있다라는 감성의 미학에서 Esthetique라는 단어를 처음 사용했다. 다시 말하면 Soim Esthetique는 인간의 마음 만족과 쾌감을 주는 배려 있는 조치로써 여성이 화장을 함으로써 외모의 조화에서 마음과 신체의 균형을 꾀하는 생명현상의 심층과 연관되는 것과 마찬가지로 에스테틱과 화장의 심리는 매우 땔래야 땔 수 없는 불과분의 밀접한 관계라 볼 수 있다.

• Journal of Life Science
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• v.18 no.6
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• pp.871-877
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• 2008

Onions are considered to be a promising source of the alcoholic drink because they are rich in sugar, amino acids and various nutrients. To isolate strains of Saccharomyces cerevisiae producing ethanol of higher concentration, 19 strains were subjected to screening. Among them, the strain producing the highest concentration of ethanol was OJ-8 strain. Onion's oder was effectively removed by treatment for 30 min with 10% (w/v) charcoal against medium and then heat treatment of onion extract for 40 min at $100^{\circ}C$. The optimum conditions for alcoholic fermentation was investigated in medium containing the onion extract. The optimal conditions for ethanol production was obtained by standing culture for 5 days at $25^{\circ}C$ with 5% inoculum volume.

• Microbiology and Biotechnology Letters
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• v.16 no.2
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• pp.136-141
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• 1988

Susceptibility of a thermophilic strain (D-71) of Saccharomyces cerevisiae and an osmotolerant strain (SR-S) of Zygosaccharomyces rouxii to Zymolyase-20T were studied in various renditions. Content of glucan and mannan in cell wall of Saocharomyces cerevisiae D-71 were 14.5% and 14.8%, and Zygosaccharomyces rouxii SR-S were 24.0% and 19.0%, respectively. Susceptibility of Saccharomyces cerevisiae D-71 cultured in Wickerham synthetic medium containing 0.5% of methionine and 0.1% of glucose to Zymolyase-20T was 66%, and $K_2$HPO$_4$ and aminobenzoic acid were greatly effective to susceptibility. Susceptibility of Zygosaccharomyces rouxii SR-S cultured in Wickerhnin synthetic medium containing 0.5% of peptone, 0.15% of methionine and 0.l% of glucose to Zymolyase-20T was 80%, and KI and pyridoxine were greatly effective to susceptibility. Susceptibility of Saccharomyces cerevisiae D-71 stationary cultured in YMPG medium at $25^{\circ}C$ for 12 hours was 16o1e and Zygosaccharomyces rouxii SR-S stationary cultured in YMPG medium at $25^{\circ}C$ for 30 hours was 82%.

• Microbiology and Biotechnology Letters
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• v.15 no.2
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• pp.135-139
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• 1987

Aspergillus sp. MK-24 was cultured at 3$0^{\circ}C$ in the medium consisting of 2% glucose, 0.2% NaNO$_3$, 0.02% $K_2$HPO$_4$, 0.02% MgSO$_4$ 7$H_2O$, 0.02% KCl, and at initial pH of 5.0. The production of the inhibitor on venom proteinase reached to the maximum in 7 days. Sodium nitrate or potassium nitrate as a nitrogen source was favorable. The production of inhibitor was not affected by the addition of most of the inorganic salt used but depressed by lead, zinc, cobalt, mercuric or silver salts.

• Microbiology and Biotechnology Letters
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• v.29 no.2
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• pp.67-71
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• 2001

Isolation and Growth Characteristics of AIkalophilic Bacillus sp. for Removal of Anthraquinone Dye. Kim, Jeong-Mog. School of Environmental Information, Taekyeung College, Kyungsan, 712-850, Korea -Alkalophilic strain degrading and decolorizing anthraquinone dye, Remazol brilliant blue R was isolated from natural system and named as Bacillus sp. ARB!. The optimal temperature and pH of Bacillus sp. ARBI were 35°C and 9.0, respectively. The pH of culture media during the fermentation were changed from 10 and 10.5 of initial values to 9.3 and 9.4 after 40 hrs, respectively. Decolorization efficiency in aerobic shaking culture of Bacillus sp. ARBI was markedly higher than that in standing culture. At the optimal culture condition, decolorization efficiency by the Bacillus sp. ARBl was 93% after 32 hrs batch culture. In the case of batch culture using real dye processing wastewater, dye decolorization efficiency of Bacillus sp. ARBl was 78% after 40 hrs.

• Microbiology and Biotechnology Letters
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• v.10 no.4
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• pp.259-265
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• 1982

These experiments were conducted to investigate the hydrolysis products on the various oligosaccharides of Bacillus cirulans F-2 $\alpha$-amylase, and the hydrolysis rate on the various raw starches of Bacillus circulans F-2 $\alpha$-amylase, Bacillus amylotiquefaciens $\alpha$-amylase and Rhizopus niveus glucoamylase. The results obtained were as follows : 1. Maltotetraose, maltopentaose, maltohexaose, maltoheptaose and maltooctaose were hydrolyzed, but maltose and maltotriose were not hydrolyzed by Bacillus circulans F-2 $\alpha$-amylase. Among maltotetraose, maltopentaose, maltohexaose, maltoheptaose and maltooctaose, especially maltotetraose was hydrolyzed weakly by Bacillus circulans F-2 $\alpha$-amylase. 2. The Hydrolysis rate of oyster glycogen was slightly lower than soluble starch, amylose and amylopectin. 3. The hydrolysis rate of com starch was higher in shaking incubation than in stationary incubation, but the hydrolysis rate of potato starch was not definite according to kinds of enzyme. 4. On com, rice, arrowroot, high amylose corn, banana, sago, yam and potato starch, Bacillus circulans F-2 $\alpha$-amylase exhibited a remarkably higher hydrolysis rate than Bacillus amyloquefaciems $\alpha$-amylase and Rhizopus niveus glucoamylase.