• Korean Chemical Engineering Research
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• v.43 no.2
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• pp.187-201
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• 2005

Bioprocessing technologies utilizing 'biorecognition' between a solid matrix and a protein is being widely experimented as a means to replacing the conventional, solution-based technology. Frequently the matrices are chromatographic resins with specific functional groups exposed outside. Since the reactions of and interactions with the proteins occur as they are attached to the solid matrix, this 'solid-phase' processing has distinct advantages over the solution-phase technology. Solid-phase refolding of inclusion body proteins uses ion exchange resins to adsorb denaturant-dissolved inclusion body. As the denaturant is slowly removed from the micromoiety around the protein, it is refolded into a native, three-dimensional structure. Once the refolding is complete, the folded protein can be eluted by a conventional elution technique such as the salt-gradient. This concept was successfully extended to 'EBA (expanded bed adsorption)-mediated refolding,' in which the denaturant-dissolved inclusion body in whole cell homogenate is adsorbed to a Streamline resin while cell debris and other impurity proteins are removed by the EBA action. The adsorbed protein follows the same refolding steps. This solid-phase refolding process shows the potential to improve the refolding yield, reduce the number of processing steps and the processing volume and time, and thus improve the overall process economics significantly. In this paper, the experimental results of the solid-phase refolding technology applied to several biopharmaceutical proteins of various types are presented.

• The Journal of the Convergence on Culture Technology
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• v.6 no.1
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• pp.381-388
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• 2020

This paper discusses English Hedges or Hedging Expressions on the basis of their morphosyntactic-pragramatic properties within the perspective of sociolinguistics. The term, 'Hedges' for the past decades since Lakoff(1973), has received little attention from the English grammar circles such as morphosyntax and the generative grammar theories. This paper presents a more comprehensive approach to the identification, distributions, functions, and the morphosyntactic properties of English Hedges. The earlier research on English Hedges in the 70's show that hedges are metalinguistic or mitadiscourse expressions which constitute a means for executing Politeness strategy in pragmatics. Nonetheless, research from the interface of syntactic-pragmatics has been scarce. This article suggests a more complex body of English hedges that have not been extensively discussed in the literature. Additionally, their configurational domain is to be proposed as part of the PolP with [±hedged] above CP⁺ (or CP beyond). The ramifications of the current study are suggested in terms of comparative linguistics, EFL/ESL studies of English for global communication, and pragmatics-sensitive machine translation studies in the forseeable future.

• Journal of The Korean Association For Science Education
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• v.37 no.5
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• pp.813-824
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• 2017

In the previous studies on co-constructing scientific models, students have had various difficulties in constructing their models. As an alternative to compensate for these problems, we introduced smart devices in the modeling process and examined its roles. 60 students from two classes in the fifth grade of an elementary school in Seoul had classes to make up the solar system model in a small group using the smart devices and we examined the roles of smart devices in their classes. The result is as follows; First, students were able to generate their scientific model through various functions of smart devices throughout the modeling process. In particular, smart devices provide highly descriptive and authoritative information that meets the needs of students as well as the opportunity to observe the planets from various perspectives. Moreover, they were not only able to express their models easily as they wanted to express them, but also were able to receive feedbacks from various agents. In order for these smart devices to function properly in the co-construction of scientific models, it is necessary to improve the related environment as well as students' technological literacy.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.16 no.6
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• pp.3807-3818
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• 2015

The objective of this study is to develop an on-line system for improving undergraduate students' competency development. After drawing elements necessary for the competency development such as assessment and planning, competency development, analysis of competency assessment, portfolio, analysis of job ability and community, based on the literature research related to competency and the analysis of the existing system, the direction of the system design was set up. The system was developed by using Microsoft Windows operating system in Windows server, ORACLE ver.10 as its database management system, and JSP and JAVA as its programing language. Reviewing errors and improvements of the system, it was modified and complemented. In order to examine the content functional utilization of the final competency development system, the utilization was verified. The competency development system for undergraduate students can be used as on-line space filled with the internalization of knowledge, self-directed competency development, convenience of record management and interactions between students-professors-alumna, owing to its functions such as boosting competency activities, cultivating career-pioneering ability and introspecting. When it is rare to find researches on the competency development system for undergraduate students, it is expected to be helpful to the development of competency education and the career education for undergraduate students as a new alternative for the competency development.

• Korean Journal of Food Science and Technology
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• v.50 no.6
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• pp.636-641
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• 2018

This study was conducted to compare the stability between branched-chain amino acid (BCAA)-encapsulated liposome and double emulsion (DE). Liposome was produced by high-speed homogenization and ultrasonication whereas DE was prepared by homogenizing with surfactants. All samples were fixed at pH 4 and 7 and stored at 4, 25, and $40^{\circ}C$ for 5 days. Encapsulation efficiency and cumulative release rate were measured under $4^{\circ}C$ and at $25^{\circ}C$. The results showed that the size of BCAA-coated liposome was greater at pH 7 than at pH 4. The zeta-potential value of BCAA-coated liposome was greater at pH 4 than at pH 7. It was supposed that the negatively charged liposomes attracted the positively charged BCAAs at pH 4 resulting in the formation of the vesicle with smaller size. Particle size of DE was smaller than $100{\mu}m$. Encapsulation efficiencies of BCAA in DE or liposome were over 97%, approximately, and the cumulative release rates of them were below 30% for 5 days.

• KIPS Transactions on Computer and Communication Systems
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• v.8 no.2
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• pp.41-56
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• 2019

Cloud manufacturing is a new concept of manufacturing process that works like a single factory with connected multiple factories. The cloud manufacturing system is a kind of large-scale CPS that produces products through the collaboration of distributed manufacturing facilities based on technologies such as cloud computing, IoT, and virtualization. It utilizes diverse and distributed facilities based on centralized information systems, which allows flexible composition user-centric and service-oriented large-scale systems. However, the cloud manufacturing system is composed of a large number of highly heterogeneous subsystems. It has difficulties in interconnection, data exchange, information processing, and system verification for system construction. In this paper, we derive the user requirements of various aspects of the cloud manufacturing system, such as functional, human, trustworthiness, timing, data and composition, based on the CPS Framework, which is the analysis methodology for CPS. Next, by analyzing the user requirements we define the system requirements including scalability, composability, interactivity, dependability, timing, interoperability and intelligence. We map the defined CPS system requirements to the requirements of oneM2M, which is the platform standard for IoT, so that the support of the system requirements at the level of the IoT platform is verified through Mobius, which is the implementation of oneM2M standard. Analyzing the verification result, finally, we propose a large-scale cloud manufacturing platform based on oneM2M that can meet the cloud manufacturing requirements to support the overall features of the Cloud Manufacturing CPS with dependability.

• Journal of Service Research and Studies
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• v.10 no.4
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• pp.101-125
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• 2020

This study was conducted to derive a management technology model based on the new management framework established in previous studies. The management technology sector, which occupies most of the existing business administration, is defined as a technical division in new management. In this study, the theory of management technology based on the service philosophy of the service economy era was presented. The structure of new business, which is the basis of new management technology, is presented first, the service philosophy of the service economy era where management is performed, and then the management technology model based on service philosophy is presented. The management technology model was derived on the basis of immutable axioms. After presenting new management axioms based on common human ideology and nature and human nature, a management technology model was presented based on axioms. On the basis of the axioms, a new dialectical development model was developed as a model for the dialectical development that maintains a tight balance and a fierce interaction between two opposing parties based on the structure and operation model of service philosophy. In addition to the overall organizational management model, a management function model and a management expansion model were presented. Each detailed technique is presented as a model for dialectical development of opposing confrontations. Management technology is a dynamic technology that is constantly changing, and is an overall technology that takes into account various situations and viewpoints. This study has significance as a basic study to overcome the limitations of the existing static management technology and develop dynamic management technology. Future research requires empirical analytical studies on new management technology models.

• Journal of Korea Entertainment Industry Association
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• v.13 no.4
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• pp.131-138
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• 2019

The film enters the color film from black and white, and the screen image changes qualitatively. The color in the film not only has the reproduction function of the restoration object like the objective appearance, but also has the function of conveying different subjective emotions. It can express the color and can't express it. The artistic effect conveys the information content that the story itself can't convey, so the color of the film becomes an important part of the film language. The color in the film is presented on the screen in the form of single-screen color, scene color, full-color color tone, and various color chains designed according to different contradictions and conflicts. Because the film art and art means are assembled by montage, he colors in the picture also form a montage form. People call it "color montage". People's subjective nature of color criticism and acceptance of color language also depend on various local tones. The accurate expression of the relationship, the unique attribute of color determines that the color must enter the structural state in order to express its unique charm. The color of the film only has the real aesthetic value when it enters the level of "color structure". This paper studies the color of Chinese and Western films from the differences between the color thinking of Chinese and Western film directors and the cultural implication of Chinese and Western film colors. The western film director emphasizes the structure of color and pays attention to the use of tonal montage to convey the characters. Emotions reflect the characteristics of a subjective color. Beginning with the "fifth-generation" director of Chinese film, the new journey of film color language has been opened. In the process of blending love and scenery, the film style of "image-in-one" has been achieved.

• Therapeutic Science for Rehabilitation
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• v.12 no.2
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• pp.25-42
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• 2023

Objective : The purpose of this study was to identify the characteristics of a single-subject research study and qualitative levels in which occupational therapy interventions were performed on children with cerebral palsy. Methods : This study targeted papers, published in Research Information Sharing Service (RISS), National Digital Science Library (NDSL), Koreanstudies Information Service System (KISS), and E-article from May 20 to 29, 2022. The search terms were 'cerebral palsy' AND 'single subject research design' OR 'individual subject study'. Eleven papers, were finally selected and analyzed. Results : Most of the studies were medium in methodological quality, and the subjects were pre-school age and spastic hemiplegia. Among the single-subject designs, intervention-removal designs were the most common, and among them, ABA designs were the most common. Interventions included assistive devices, constraint-induced therapy, neurodevelopmental therapy, and sensory integration therapy were 2, and upper extremity exercise, interactive metronome, and CO-OP were 1. Dependent variables were measured with 2 to 4 measurement tools, Significant improvements were found in postural control ability, gait and balance, hand function, and upper extremity function. Conclusion : This study confirmed that it is helpful to apply cerebral palsy occupational therapy by presenting the characteristics of cerebral palsy, intervention sessions and effects, measurement tools and methodological quality levels.

• The Korean Journal of Franchise Management
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• v.3 no.1
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• pp.73-91
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• 2012

This study is in the purpose for the revitalization of traditional market as comparing to the relevancy between the central characteristics of a floating population going around for buying something or eating food and lots of small-sized businesses comprising of the commercial districts. The several traditional markets such as Cheonan station, Dujeong-dong, Sinbu-dong in Cheon-An city has been investigated repeatedly almost every two or three years by the Small Enterprise development Agency(SEDA) since 2001. By analyzing the raw data of those commercial districts made by SEDA, we can calculate the number of firms andthe ratio of business type of each commercial districts. In this research, the type of each business is classified into four groups such as restaurant, service, retail and the rest. Moreover, the central character of the floating population is derived from the raw data, which means the customer information about sex, age structure or the most populous time zones. From these characteristics, one commercial districts has his own specific features distinguishing from the others. The most important differences of past researches are firstly the dynamic viewpoint rather than a static one. Secondly it suggests that the relation between the central characteristics of districts and the floating population would exist. Lastly, it suggests that the interaction between both of them have a significant effect on the growth or decline of the districts and the rates of business type, other adjacent commercial districts as well. Eventually, this study provides several meaningful points for the revitalization of commercial districts to government or stakeholder such as management organization, business owners and new starter etc.