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Development of Optical Molecular Imaging System for the Acquisition of Bioluminescence Signals from Small Animals (소동물 발광영상 측정을 위한 광학분자영상기기의 개발)

  • Lee, Byeong-Il;Kim, Hyeon-Sik;Jeong, Hye-Jin;Lee, Hyung-Jae;Moon, Seung-Min;Kwon, Seung-Young;Choi, Eun-Seo;Jeong, Shin-Young;Bom, Hee-Seung;Min, Jung-Joon
    • Nuclear Medicine and Molecular Imaging
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    • v.43 no.4
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    • pp.344-351
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    • 2009
  • Purpose: Optical imaging is providing great advance and improvement in genetic and molecular imaging of animals and humans. Optical imaging system consists of optical imaging devices, which carry out major function for monitoring, tracing, and imaging in most of molecular in-vivo researches. In bio-luminescent imaging, small animals containing luciferase gene locally irradiate light, and emitted photons transmitted through skin of the small animals are imaged by using a high sensitive charged coupled device (CCD) camera. In this paper, we introduced optical imaging system for the image acquisition of bio-luminescent signals emitted from small animals. Materials and Methods: In the system, Nikon lens and four LED light sources were mounted at the inside of a dark box. A cooled CCD camera equipped with a control module was used. Results: We tested the performance of the optical imaging system using effendorf tube and light emitting bacteria which injected intravenously into CT26 tumor bearing nude mouse. The performance of implemented optical imaging system for bio-luminescence imaging was demonstrated and the feasibility of the system in small animal imaging application was proved. Conclusion: We anticipate this system could be a useful tool for the molecular imaging of small animals adaptable for various experimental conditions in future.

The Effects of Simultaneous Application of Peripheral Nerve Sensory Stimulation and Task-Oriented Training to Improve Upper Extremity Motor Function After Stroke: Single Blinded Randomized Controlled Trial (뇌졸중 환자의 상지기능 개선을 위한 말초감각신경자극과 과제 지향적 훈련의 동시 적용 효과: 단일 맹검 무작위대조군실험)

  • Kim, Sun-Ho;Won, Kyung-A;Jung, Eun-Hwa
    • Therapeutic Science for Rehabilitation
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    • v.9 no.4
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    • pp.7-20
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    • 2020
  • Objective : This study aimed to investigate the effect of simultaneous application of peripheral nerve sensory stimulation and task-oriented training on the improvement of upper extremity motor function after stroke. Methods : This study included 29 patients with hemiplegia. The 14 subjects were in the peripheral nerve sensory stimulation and task-oriented training group for 4 weeks (30 min/d, 5 d/wk), while the 15 control group subjects underwent only task-oriented training for the same duration. The outcome measures were the percentage of voluntary baseline muscle contractions of the wrist and shoulder and Box and Block Test, grip and pinch strength, and Action Research Arm Test. Results : After 4 weeks, muscle activity of extensor carpi radialis, flexor carpi radialis and grip strength and Action Research Arm Test were significantly higher in the experimental group. Conclusion : Simultaneous application of the peripheral nerve sensory stimulation and task-oriented training was found to be superior to task-oriented training for improving upper extremity motor function of adults with stroke.

Interactive Statistics Laboratory using R and Sage (R을 활용한 '대화형 통계학 입문 실습실' 개발과 활용)

  • Lee, Sang-Gu;Lee, Geung-Hee;Choi, Yong-Seok;Lee, Jae Hwa;Lee, Jenny Jyoung
    • Communications of Mathematical Education
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    • v.29 no.4
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    • pp.573-588
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    • 2015
  • In this paper, we introduce development process and application of a simple and effective model of a statistics laboratory using open source software R, one of leading language and environment for statistical computing and graphics. This model consists of HTML files, including Sage cells, video lectures and enough internet resources. Users do not have to install statistical softwares to run their code. Clicking 'evaluate' button in the web page displays the result that is calculated through cloud-computing environment. Hence, with any type of mobile equipment and internet, learners can freely practice statistical concepts and theorems via various examples with sample R (or Sage) codes which were given, while instructors can easily design and modify it for his/her lectures, only gathering many existing resources and editing HTML file. This will be a resonable model of laboratory for studying statistics. This model with bunch of provided materials will reduce the time and effort needed for R-beginners to be acquainted with and understand R language and also stimulate beginners' interest in statistics. We introduce this interactive statistical laboratory as an useful model for beginners to learn basic statistical concepts and R.

Be Age-dating of Marine Sediments from NE Pacific (북동태평양 해저퇴적물의 Be 연대측정)

  • Choi, Hun-Soo;Kong, Gee-Soo;Um, In-Kwon;Kim, Ji-Hoon
    • Journal of the Mineralogical Society of Korea
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    • v.24 no.3
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    • pp.179-188
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    • 2011
  • In order to investigate absolute age of marine sediment in the KR5 region, northeastern Pacific, we measured the Be isotope ($^{10}Be$ and $^{9}Be$) of box core (BC08-02-13) sediment with depth. Core sediment is divided into three sedimentary facies (Facies I, Facies II, FaciesIII). Facies I mainly consists of brown to dark brown (10YR4/3) homogeneous mud with high water content. Facies II shows brownish yellow (10YR6/6) color. The unconformity is recognized at the boundary between Facies I and Facies II, Facies III consists of very dark brown (10YR2/2) mud. Many bioturbated burrows are observed at FaciesII and FaciesIII. Based on Be dating results, FaciesIII and Facies II had been deposited before 3.7 Ma and 2.3 Ma, respectively. After 2.3 Ma, the upper part of Facies II was eroded due to the change of sedimenary environment. Facies I has been deposited since 1.8 Ma. The unconformity located at the boundary between Facies I and Facies II may be driven by the envrionmental change due to the migration of Intertropical Convergence Zone.

Analysis of Agricultural Climatology in Cheju Island I. Distribution of Daily Minimum Temperature in Winter Season Estimated from a Topoclimatological Method (제주도의 농업기후 분석 I. 지형기후 추정법과 동계 일최저기온 분포)

  • 윤진일;유근배;이민영;정귀원
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.34 no.3
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    • pp.261-269
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    • 1989
  • Agricultural activities in Chejudo require more specialized weather services in this region. The meteorological information available from the Korea Meteorological Service (KMS) is limited in its areal coverage because the KMS stations are located along the narrow band of coastal area. topoclimatological technique which makes use of empirical relationships between the topography and the weather can be applied to produce reasonable estimates of the climatic variables such as air temperature and precipitation over remote land area where routine observations are rare. Presentation of these estimates in a from of fine-mesh grid map can also be helpful to upgrade the quality of weather services in this region. Altitude values of the 250 m grid points were read from a 1: 25000 topographic map and the mean altitude, the mean slope, and the aspect of the slope were determined for each 1 km$^2$ land area from these altitude data. Daily minimum air temperature data were collected from 18 points in Chejudo during the winter period from November 1987 to February 1988. The data were grouped into 3 sets based on synoptic pressure pattern. Departures from the KMS observations were regressed to the topographical variables to delineate empirical relationships between the local minimum temperature under specific pressure patterns and the site topography. The selected regression equations were used to calculate the daily minimum temperature for each 1 km$^2$ land area under the specific pressure patterns. The outputs were presented in a fine-mesh grid map with a 6-level contour capability.

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Effects of Seeding Rates on the Endosperm Depletion and Seedling Growth of Rice for Mechine Transplanting (수도기계이앙육묘에 파종량이 배유물질의 소모 및 묘소질에 미치는 영향)

  • 김용재;신해룡;송동석;장강운
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.33 no.2
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    • pp.146-156
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    • 1988
  • This study was carried out to investigate the change of endosperm substance and seedling growth in rice seedlings for machine transplanting with use of two rice varieties, which Samgangbyeo (Indica${\times}$Japonica) and Dogjinbyeo (Japonica) were sown on May 8 with five levels of seeding density. 1. The total dry matter weight, leaf area, length of the longest root, number and length of new roots were negatively correlated with seeding density, and the plant height had the similar tendency to them from 20 days after sowing(DAS). 2. The rooting ability was negatively correlated with seeding density and decreased from 35 DAS in Dongjinbyeo. 3. The endosperm substance were exhausted at 25 to 30 DAS in Samgangbyeo and at 25 DAS in Dongjinbyeo. 4. The leaf area was more useful than the ratio of plant height to dry matter weight for judging the constitution of seedlings. 5. The reducing sugar contents in the endosperm were the greatest among parts of rice seedlings followed by leaf and root. The contents in leaf, root and endosperm reached maximal at 10 DAS, and varied with plant parts, seedling growth and varieties in different levels of seeding density. 6. The protein contents in leaf and root of rice seedlings became decreased with seedling growth., and were minimal values at 15 DAS. The content in endosperm was decreased soon after seeding, and minimal at 4 DAS with showing some fluctuation of protein content after then.

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Effects of Pretreatments and Storage Conditions on Quality and Vase Life of Cut 'Red Sandra' Rose (전처리와 저장조건이 절화장미 'Red Sandra'의 품질과 수명에 미치는 영향)

  • Bang, Chang-Seok;Song, Cheon-Young;Lee, Jong-Suk;Huh, Kun-Yang;Song, Jeong-Seob
    • Horticultural Science & Technology
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    • v.17 no.6
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    • pp.762-764
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    • 1999
  • This experiment was conducted to investigate the effect of pretreatments and storage conditions on quality and vase life of cut 'Red Sandra' rose (Rosa hybrida L.). 'Red Sandra' rose flowers were harvested and pulsed with distilled water, 0.2% RVB or 200ppm aluminum sulfate+3% sucrose+50ppm $AgNO_3$+0.1mM ethionine for 22 hours and then stored in wet or dry condition for 12, 24, 48 hours at low or room temperature. Pulsing with aluminum sulfate+sucrose+$AgNO_3$+ethionine delayed bent-neck, increased solution absorption and flower diameter, and prolonged vase life rather than pulsing with distilled water or RVB. Storage in wet condition or low temperature also delayed bent-neck, increased solution absorption and flower diameter, and prolonged vase life compared with storage in dry or room temperature condition. As the storage hours prolonged, solution absorption, flower diameter, and vase life were decreased especially pulsing with distilled water. Pulsing with aluminum sulfate+sucrose+$AgNO_3$+ethionine prolonged vase life compared to pulsing with distilled water or RVB regardless of storage conditions and shipping hours.

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Establishment of Distribution System by Individual Packaging of Live Todarodes pacificus (활오징어(Todarodes pacificus)의 개별 포장 유통시스템 확립)

  • Kim, Myung Uk;Cho, Young Je
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.43 no.7
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    • pp.1095-1103
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    • 2014
  • A packaging and distribution system for transferring individual live squids at low temperature was developed and compared to a conventional bulk container system. Ten live squids in individual packages were stored in a large container at low temperature ($0{\sim}10^{\circ}C$). Live squids in individual packages at $6^{\circ}C$ showed a survival rate of 84% up to 72 hours, after which the survival rate decreased. However, the survival rate remained at 60% up to 120 hours. Further, the squids survived up to a maximum of 7 days. Optimum temperature was $5^{\circ}C$, and the survival rate of the packages was 70% when stored at $5^{\circ}C$ for 96 hours. A distribution test was carried out using a refrigerator truck at $5^{\circ}C$, and the results showed a 100% survival rate up to 16 hours and over 90% survival rate after 20 hours. A rectangular container was the most favorable when loading the container into the refrigerator truck. In testing the required volume of supplied seawater, 100% survival rate was observed over 15 hours with 20 L of sea water or more. Therefore, a single squid needed 2 L of seawater. After refrigerator truck transportation, optimum temperature for fish tank storage was $5^{\circ}C$, at which the survival rate was over 90% up to 72 hours. Using a refrigerator truck at $5^{\circ}C$, live squids survived up to 7 days, maintaining marketability.

Effect of Simultaneous K and Ca Application to Paddy Soil on the Uptake of Radiocaesium and Radiostrontium by Rice (논토양에 대한 K와 Ca의 동시처리가 벼의 Radiocaesium과 Radiostrontium 흡수에 미치는 영향)

  • Choi, Yong-Ho;Lim, Kwang-Muk;Jun, In;Keum, Dong-Kwon;Kim, In-Gyu
    • Journal of Radiation Protection and Research
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    • v.37 no.2
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    • pp.90-95
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    • 2012
  • A radio-tracer experiment was performed in a greenhouse to investigate the effectiveness of the simultaneous application of K and Ca as a countermeasure for reducing the radiocaesium and radiostrontium uptake by rice. Paddy soil (loam of pH 6.5) in soil boxes was spiked with $^{137}Cs$ and $^{85}Sr$, and treated with K and Ca in the forms of KCl and $Ca(OH)_2$, respectively, at agrochemical grades before transplanting. For the seeds of the control plants, soil-to-plant transfer factors (TF, $m^2\;kg^{-1}-dry$) of $^{137}Cs$ and $^{85}Sr$ were $7.4{\times}10^{-5}$ and $2.1{\times}10^{-4}$, respectively, whereas the corresponding values for the straws were $2.6{\times}10^{-4}$ and $2.2{\times}10^{-2}$, respectively. The TF values of $^{137}Cs$ and $^{85}Sr$ kept decreasing as the level of the simultaneous application of K and Ca (K/Ca, $g\;m^{-2}$) increased up to 33.6/322 and 48.0/460, respectively. The maximum rate of the decrease was around 60% for both radionuclides. Nearly 60% reduction in the TF value of $^{85}Sr$ was observed even at the dosage of 33.6/322, which was considered the optimum dosage based on crop productivity as well as reduction in the radiocaesium and radiostrontium uptake by rice. The optimum dosage may depend on various factors so further experiments need to be made for many different conditions.

[Mössbauer] Spectroscopic Study of La1/3Sr2/3FeO2.96 under the External Magnetic Field (산소결핍 페롭스카이트 La1/3Sr2/3FeO2.96의 외부 자기장 하에서의 Mössbauer분광학적 연구)

  • Yoon, Sung-Hyun;Jung, Jong-Yong
    • Journal of the Korean Magnetics Society
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    • v.15 no.2
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    • pp.81-84
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    • 2005
  • The origin for the charge disproportionation (CD) transition in polycrystalline $La_{1/3}Sr_{2/3}FeO_{2.96}$ was examined using X-ray diffraction and the external field $M\ddot{o}ssbauer$ssbauer spectroscopy. In order to see how the external magnetic field affects the CD state above its transition temperature, an external magnetic field of up to 6 T was applied either parallel or perpendicular to the $\gamma-ray$ direction with the sample temperature fixed at 225 K, which was above the CD transition temperature. Without an external magnetic field, a completely paramagnetic singlet was obtained in the temperature range of the averaged valence state above the transition temperature, which was interpreted as coming from the average valence $Fe^{3.6+}$. In the longitudinal geometry, a magnetic Zeeman with its intensity ratio 3:0:1:1:0:3 is superimposed to the central singlet. In the transverse geometry, however, the central singlet disappears and only a magnetic component with its intensity ratio 3:4:1:1:4:3 emerges. The existence of a singlet is understood as an evidence of the fast electron-transfer among Fe ions. Since the singlet still exists under the magnetic field, the application of an external field has little effect on the conduction mechanism of hopping electrons.