• Proceedings of the Korean Vacuum Society Conference
• /
• 2011.08a
• /
• pp.335-335
• /
• 2011

곰팡이에 의한 감염과 바이오 필름 형성이 우리 인간에게 매우 유해하다는 것은 익히 알려진 바이다. 일례로, 이는 우리가 먹는 음식을 오염시키고 치아를 상하게 하며 수도관을 부식시키고 또 우리 몸속에 집어넣는 의료기기에서 자라 심각한 질병을 유발시키는 등 여러가지 문제를 일으킨다. 곰팡이 감염과 바이오 필름 형성을 막기 위해 항생제 및 화학물질 등을 이용하는 방법들이 있으나 저항성이 증가된 균주의 출현, 환경오염 등의 문제점을 유발시키고 있다. 따라서 좀더 지속적이고 환경친화적인 기술개발이 요구되어지고 있다. 강한 살균력이 있다고 알려져 있는 대기압 플라즈마는 이러한 기술개발에 적용해볼 필요가 있다. 본 연구에서는 상온 대기압 플라즈마를 만들어 내는 여러 타입의 장치를 이용하여 곰팡이 살균과 이스트 바이오필름 형성 저해에 대한 조사를 하였고 플라즈마 처리에 따른 곰팡이 세포들의 세포학적 분자생물학적 영향에 대해 조사해 보았다. 대기압 상온 플라즈마 제트는 대기중에서 방안의 온도로 플라즈마가 생성되며 이를 페트리디쉬위에 접종된 이스트(Saccharomyces cerevisiae) 위 10 mm에서 조사한 결과 2분 이상의 처리를 했을 때 바이오 필름형성을 하지 못함을 보였고 곰팡이(Neurospora crassa)포자에 처리했을 때는 처리시간에 비례하여 발아하는 포자수가 감소하고 포자의 모양도 수축되는 것으로 관찰되었다. 대기압 플라즈마 제트는 대면적이 아닌 국부적 살균으로 살균력은 보이나 그 효율성이 낮아 이를 보완하기위해 DBD 형식의 플라즈마 장치를 만들었으며 이는 페트리디쉬의 모든 면을 동시에 조사할 수 있는 장점이 있다. 다만 제트와는 달리 플라즈마의 생성구간이 넓고 얇아 시료와 2~3 mm 간격을 띄우고 조사하였다. 제트와 마찬가지로 살균의 효과를 보였으며 기존의 단점을 극복하여 동시에 더 많은 면적을 조사 할 수 있게 되었다. 이를 통해 플라즈마의 살균능력을 직접 확인하였고 앞으로 더 많은 실험과 연구를 통해 일련의 과정들에 대한 원인을 규명하고자 한다.

• The Hankook-Saengyark Bo
• /
• no.233
• /
• pp.5-5
• /
• 1999

밀리타리스 동충하초 재배법 - 초피나무 새소득원 유망(열매$\cdot$잎 살균력 우수 비타민도 다량함유) - '가시오갈피' 미국수출 초림농산$\cdot$진액으로

• Journal of Food Hygiene and Safety
• /
• v.37 no.5
• /
• pp.317-322
• /
• 2022

Corynebacterium pseudotuberculosis is the causative agent of caseous lymphadenitis (CLA), a chronic contagious disease in small ruminants. The prevalence of CLA has been reported to be >50% in Korean black goats. CLA is difficult to control due to a lack of efficient vaccines and treatment methods. Effective disinfection of the farm environment may be an alternative strategy for reducing the spread of C. pseudotuberculosis. The objective of this study was to evaluate the efficacy of commercial disinfectants against CLA. The six commercial disinfectants, largely composed of sodium dichloroisocyanurate, sodium hypochlorite, potassium monopersulfate triple salt, quaternary ammonium, citric acid, and copper sulfate, were tested against five different genotypes of C. pseudotuberculosis isolated from goat farms in Korea. Efficacy tests were performed in accordance with the disinfectant efficacy test guidelines recommended by the Animal and Plant Quarantine Agency of Korea with slight modifications. All disinfectants except for copper sulfate exhibited >99.99% killing efficacy under hard water conditions following 30 min of incubation, which is the recommended standard treatment time according to guidelines. The minimum bactericidal treatment time was evaluated by employing treatments for durations of 1, 5, and 15 min. The most effective compounds under hard water conditions were sodium dichloroisocyanurate, potassium monopersulfate triple salt, and sodium hypochlorite, exhibiting >99.99% killing efficacy after 1 min of treatment. In the aqueous solution forms, citric acid and the quaternary ammonium compound were the most effective, but required at least 5 min to kill >99.99% of the bacteria. The current study characterizes the killing efficacy of six commercial disinfectant active compounds against C. pseudotuberculosis. Thus, this study provides essential information regarding the efficacy of the disinfectants used to control CLA in goat farms.

• Journal of Food Hygiene and Safety
• /
• v.29 no.2
• /
• pp.117-122
• /
• 2014

Rice can be the contaminating with soil-borne bacteria. Furthermore, the contaminated bacteria can be grown during immersion process for produce wet-milled rice flour. Therefore, disinfectants can be added during the immersion process. Antibacterial activities of the natural disinfectant, fermented rice spent water (FRSW), and the chemical disinfectants, chlorine dioxide (CD) and sodium benzoate (SB), were respectively determined when added in pure cultures of target bacteria such as Salmonella typhimurium, Escherichia coli, and Bacillus cereus or when added to immersion water in the immersion process. In addition, rinsing effects for removing bacteria were determined when rice was rinsed with water before and after the immersion process. Antibacterial activities were rapidly increased as increasing amounts of the disinfectants are added to the pure cultures of the target bacteria. Antibacterial activity of CD was the most effective among the three tested disinfectants when added to the pure cultures of the target bacteria, respectively. Those of the same disinfectants were increased when they were increasingly added to the immersion water. However those of the disinfectants were less effective when added to the immersion water. On the other hand, rinsing effects for removing bacteria were the most effective when rice was rinsed only with water without the immersion process. Collectively, rinsing rice with water only was more effective than using disinfectants in the immersion water during rice flour production.

• Journal of Food Hygiene and Safety
• /
• v.31 no.6
• /
• pp.471-475
• /
• 2016

Salmonella Typhimurium (S. Typhimurium) is one of the common food pathogens which may cause gastroenteritidis in human and animals. The microorganism also causes the economic loss in animal farming and food industry. In this study, the disinfection efficacy of a powder disinfectant containing phosphate compounds as a main ingredient, was evaluated against S. Typhimurium. A bactericidal efficacy test by broth dilution method was used to determine the lowest effective dilution of the disinfectant following exposure to test bacteria for 30 min at $4^{\circ}C$. The disinfectant and test bacteria were diluted with hard water (HW) or organic matter suspension (OM) according to treatment condition. On HW and OM conditions, the bactericidal activity of the disinfectant against S. Typhimurium was 4- and 2-fold dilutions, respectively. As the disinfectant composed to phosphate compounds possesses bactericidal efficacy against pathogenic bacteria such as S. Typhimurium, the powder disinfectant can be used to control the spread of bacterial diseases.

• Journal of Bio-Environment Control
• /
• v.25 no.1
• /
• pp.49-56
• /
• 2016

This study was conducted to develop the disinfection system using electric shock for recycled nutrient solution in recycling soilless culture. Stainless steel (SUS 316) was found as the most appropriate electrode material for electrical disinfection system from the view of high electrical conductivity, low electric resistance, and low price. There were no changes in nutritional elements when the electric shock passed through the nutrient solution by stainless steel electrode. The amount of electric current increased with width than thickness of the electrode. The farther the distance between the electrodes was increased the time to reach out the aimed amount of current. The electric shock was applied to Ralstonia solanacearum and Fusarium oxysporum as representative bacteria and also fungi. Any of those pathogens were killed with the percentage of higher than 97% in the condition of 15VDC or 24VDC.

• Proceedings of the Korean Society of Disaster Information Conference
• /
• 2017.11a
• /
• pp.271-272
• /
• 2017

자외선 에너지는 미생물의 DNA를 파괴함으로써 살균 효과를 일으킨다. 자외선 방사로 공기 중 미생물에 대하여 높은 살균력을 얻기 위해선 강한 자외선 강도와, 흐르는 공기가 충분한 시간 동안 강한 자외선에 노출되어야 하므로 균일한 자외선 방사는 매우 중요하다. 기존 UV살균 시스템은 강한 자외선 에너지와 공기의 빠른 이동에 필요한 UV방사의 균일성을 제공하지 않았다. 하지만 HDS의 BioProtector 제품에 사용된 Advanced UV System(AUVS) 반사 캐비티 기술은 매우 높은 수준의 균일성을 생성함으로써 이 문제에 대한 해결책을 제공하며, 미생물 살균에 가장 효과적인 UV파장을 사용하여 6 LOG KILL (100만마리의 미생물 투입시 1마리 이하생존) 수준의 살균 효과를 만들었다.

• The Korean Journal of Pesticide Science
• /
• v.10 no.3
• /
• pp.165-171
• /
• 2006

A new 3-amino-1,3-thiazoline chemical library was synthesized through parallel synthetic technology and in vivo antifungal activity of the compounds were investigated against the typical 6 plant diseases (100 ppm). The characteristic feature of these derivatives was that both a benzyl moiety in C-2 imino and an amino group in C-3 of 2-imino-1,3-thiazoline scaffold were substituted in the molecule respectively. Some compounds showed antifungal activity with selectivity against tomato late blight and rice blast. The fungitoxicity would be attributed to 3,4-dichlorophenyl moiety of the benzyl group.

• Journal of Food Hygiene and Safety
• /
• v.26 no.3
• /
• pp.203-208
• /
• 2011

This study was to evaluate the efficacy of sanitizer concentrations and treatment time against two major toad-borne pathogenic microorganisms such as Escherichia coli and Staphylococcus aureus on a stainless steel surface. As a result, stainless steel, treated with 100 ppm of chlorine showed reduction of E. coli(1.56, 1.49, 1.95 log cfu/25 $cm^2$) and S. aureus(0.49, 0.88, 1.27 log cfu/25 $cm^2$) after 0, 5 and 10 min, but none was not detected in treatment with 200 ppm. The population of E. coli(0.73, 0.90, 1.55 log cfu/25 $cm^2$) and S. aureus(0.37, 1.00, 1.45 log cfu/25 $cm^2$) reduced in 35.5% ethanol treated group, but none was not detected in treatment with 70%. The population was reduced E coli(0.28, 0.64, 1.07 cfu/25 $cm^2$) and S. aureus(0.53, 0.87, 0.99 log cfu/25 $cm^2$) by treatment with 45.5 ppm of hydrogen peroxide, but none was not detected in treatment with 91 ppm. Quarternary ammonium compound with 100 ppm was reduced E. coli(0.82, 1.62, 1.71 log cfu/25 $cm^2$) and S. aureus(0.46, 0.93, 1.38 log cfu/25 $cm^2$), but none was not detected in treatment with 200 ppm. Predictive models of sterilization for all 4 disinfectants were suitable to use with $r^2$ value of higher than 0.94. These models may be of use to food services and manufacture of safe products by controlling E. coli and S. aureus without the need for further detection of the organisms.

• Journal of agriculture & life science
• /
• v.44 no.6
• /
• pp.147-157
• /
• 2010

This study was carried out to develop the normal temperature-circulatable retort sterilized imitation crab leg (RSIC) with long shelf-life by retort sterilization. We have examined the optimum retort sterilization conditions and quality characteristics of the RSIC. Central composite design for response surface methodology (RSM) was adopted for optimization of retort sterilization, and experimental central composite design consisted of 11 samples such as 4 factorial points; 4 star points; 3 central points. Sterilization temperature and $F_0-value$ for retort sterilization were chosen for independent variables, and shearing force, whiteness and sensory score about texture were selected as a dependent variable. As a results of RSM analysis, multiple response optimization for the RSIC by Minitap statistical programing was $F_0-value$ 3.3 min. at $117.5{^{\circ}C}$. Also RSM analysis indicated such as sterilization temperature during retort sterilization was the most influential factor, while $F_0-value$ little affected on quality of the RSIC.