• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.33 no.2
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• pp.85-89
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• 1997

한국서해안 격열비열도 근해산 문치가자미의 연령과 성장을 조사하기 위하여 1992년 9월부터 1993년 8월까지 매월 정기채집한 256개체의 이석을 연령형질로서 사용하여 조사 분석한 결과를 보면, 이석의 투명대와 불투명대는 년 1회 형성되었으며 투명대 형성시기는 7월이고 불투명대 형성 시기는 2월에서 4월로 이는 산란종기(2월)와 일치함을 보였다. 연령별 체장 (LT)과 연령(t)을 von Bertalanffy 성장식에 적용하여 수컷은 Lt=313.7[1-exp{-0.4342(t+0.9017)}, 암컷은, Lt=458.1[1-exp{-0.2368(t+0.1934)}과 같은 관계식을 구하였다. 또한 암컷과 수컷의 성장속도는 2세까지는 별다른 차이를 보이지 않았으나, 3세어 이상에서는 암컷이 수컷보다 월등히 빠름을 나타내었다. 본 연구에서의 최고령어는 암수 각각 5세와 6세였다.

• Korean Journal of Poultry Science
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• v.37 no.2
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• pp.139-143
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• 2010

Quail is a very useful animal model for studying vertebrate development because of its small body size and unique reproductive traits. This species is also ideal model for producing germline chimeras via transferring exogenous primordial germ cells (PGCs) into the recipient embryo. To increase the contribution efficiency of donor PGCs into recipients' tissues, decreasing the population of endogenous PGCs has been rate-limiting factor. We therefore conducted this study to investigate if gamma ($\gamma$)-irradiation depletes endogenous PGCs in developing quail embryo. Firstly, freshly laid stage X quail embryos were irradiated with various output of $\gamma$-irradiation and its teratogenic effect on the embryo was evaluated. Although a dose-dependent increase in the number of embryo showing malformation was found as the output increased (0, 250, 500, 750, and 1,000 rads), only a maximum of 10.1% of embryos were abnormal in 1,000 rads. Immunocytochemical analysis using the QCR1 antibody, which is specific marker for quail PGCs, was conducted to analyze the effect of sterilization. As results, $\gamma$-rays at a dose-rate of 500 rads/73 sec onto undeveloped stage X embryo significantly reduced the number of germ cells to an average of 75.55 % and 82.03 % in male and female embryos, respectively. We conclude that $\gamma$-ray selectively targets PGCs while affects minimally to the somatic development in quail embryo. Our results will not only provide important data for germline chimera production but can be used for analyzing the effect of ionized rays on the differentiating germ cells in various stages during animal development.

• Journal of Sericultural and Entomological Science
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• v.4
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• pp.41-46
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• 1965

Author investigated the effects of a grate type mounting on. the pupae, moth and deposit of silkworm eggs. The results are as follows. 1) The grate type mounting was more useful for the silkworms to spin the cocoons transversely than the other type. 2) Many abnormal pupae appeared in the control, and in next generation all the silkworm characters of them were poor. 3) The fact which many dead pupae occurred in the control seems to be that the cocoons spun vertical1y did harm to the physiological reactions of the pupae. 4) The moth rate emerging out of the cocoons was higher in the grate type mounting than in the control. 5) Many moths which laid normal eggs were in the grate type mounting, and also laid a lot of eggs. Therefor, author made an infference that the grate type mounting had accelerated the laying eggs. 7) The total weight of eggs produced from 1000 cocoons was heavier in the grate type mounting(index=100) than in the control (index=85). As shown in table of analysis of vanriace, above phenomenon might be relative with one of the reasons why the cocoon spun transversely could be more produced in the grate type mounting than in the control. 8) Even though the grate type mounting costs higher than the other one, it may be available because the egg weight per unit was considerably heavier in its treatment. So the cost of egg production will become cheaper.

• Microbiology and Biotechnology Letters
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• v.40 no.3
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• pp.274-277
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• 2012

Cytokinesis is the final stage of cell division, dividing one mother cell into two daughter cells. For the cutting of a plasma membrane during bacterial cytokinesis, a tubulin homolog FtsZ protein is recruited from the cytoplasm to the division site. FtsZ protein polymerizes in a GTP-dependent manner and its N-terminal domain has a GTPase activity. In this study, we have begun to characterize FtsZ from Staphylococcus aureus (SA). Full-length SA FtsZ was cloned into pRSFDuet-1 vector and the clone was transformed into a BL21 (DE3) star cell. The recombinant SA FtsZ protein was purified using Ni-NTA affinity chromatography and dialysis. Using a spectrofluorometer, we showed that SA FtsZ undergoes a GTP-dependant polymerization in vitro. The polymer of the SA FtsZ protein disappeared after a few minutes, suggesting that the polymer is degraded as the GTP is consumed. This assay system may well be applied for inhibitor screening targeting S. aureus FtsZ.

• Korean Journal of Poultry Science
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• v.37 no.2
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• pp.125-130
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• 2010

This study was conducted to estimate heritabilities, genectic and phenotypic correlations on economic traits in three strains of Korean Native chickens (KNC) : Black (B), Red Brown (RB) and Yellow Brown (YB). The data used in this study were collected from 2000 to 2007 in the National Institute of Animal Science and a total number was 14,421 birds. The effect of the strains analysis showed that the strain B were greater than the other strains for body weight at 150 days and 270 days. The number of egg production at 270 days of the strain YB was higher than the other strains. The estimated heritabilities age at $1^{st}$ egg, body weight at 150 days, egg weight at $1^{st}$ egg, body weight at 270 days, egg weight at 270 days and number of egg production at 270 days were 0.32, 0.50, 0.31, 0.43, 0.48 and 0.69 for strain B; were 0.42, 0.41, 0.19, 0.48, 0.44 and 0.76 for strain RB; were 0.44, 0.52, 0.24, 0.54, 0.51 and 0.66 for strain YB respectively, The genetic and phenotypic correlations were also estimated.

• Korean Journal of Agricultural Science
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• v.17 no.2
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• pp.87-94
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• 1990

This study was conducted to estimate the genetic and phenotypic correlations between economic traits and egg compositions, egg qualities in Korean Native Ogol fowl. The data analysis were the records of 450 pullets produced from 150 dams and 20 sires of Korean Native Ogol fowl at Chungnam National University from June 18, 1987 to April 6, 1989. The results obtained are summarized as follows : 1. The genetic correlation coefficients between the economic traits and egg components were as follows ; between body weight and albumen weight, yolk weight, shell weight were 0.083~0.591, 0.110~0.541 and 0.336~0.782 ; between age at first egg and albumen weight: yolk weight, shell weight were 0.476~0.692, 0.265~0.631 and 0.420~0.519 ; between number of egg production and albumen weight, yolk weight, shell weight were -0.578~-0.240, -0.255~-0.060 and -0.477~-0.313 ; between egg weight and albumen weight, yolk weight, shell weight were 0.825~0.939, 0.382~0.564 and 0.374~0.937, respectively. 2. The genetic correlation coefficients between economic traits and egg qualities were as follows : between body weight and egg shape index, shell thickness, albumen height, Haugh units were 0.215~0.337, 0.248~0.650, 0.161~0.624 and 0.157~0.499 ; between number of egg production and egg shape index, shell thickness, albumen height, Haugh units were -0.384~-0207, -0.557~-0.306, -0.555~-0.198 and -0.582~-0.974 ; between egg weight and egg shape index, shell thickness, albumen height, Haugh units were 0.276~0.697, 0.290~0.627, 0.238~0.538 and -0.207~0.020, respectively.

• Korean Journal of Ichthyology
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• v.13 no.2
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• pp.117-122
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• 2001

Three species, Gymnogobius urotaenia, G. sp. 1 and G. sp. 2, collected at Hosan-river, Hosan-ri, Samcheock-si, Gangwon-do, Korea, were examined for the study of sexual dimorphism. The three species have peculiar nuptial pigmentation during the breeding season: all females of the three species showed an intense black coloration on the ventral part of the head, the pelvic fin, the anal fin and the margin of the first dorsal fin. There was a difference between G. sp. 1 and the other two species in 1/3 from margin of first dorsal fin. Namely, G. sp. 1 showed an intense black coloration, whereas the other two species had an intense yellow coloration. All females of the three species showed the same, intensely yellow coloration in the ventral part of the body. Males of G. urotaenia and G. sp. 2 had almost the same nuptial pigmentation, however the former showed diffuse black coloration in the anal fin, whereas the latter had an intensely black anal fin. Males of G. sp. 1 differed from those of the other two species in their yellow color on the ventral part of the head, the pelvic f ins, the anal fin, in contrast to the diffuse black coloration in those parts in the other species. The genital papilla of males was triangle-shaped, but that of females was heart-shaped. Significant differences between sexes include: G. urotaenia, 7 characters (body depth, head length, snout length, upper jaw length, head depth, pectoral fin ray length, eye diameter); G. sp. 1, 11 characters (body depth, head length, caudal peduncle length, caudal peduncle depth, snout length, upper jaw length, head width, head depth, anal fin ray length, pectoral fin ray length and caudal fin ray length); G. sp. 2, 10 characters (body depth, head length, caudal peduncle depth, snout length, upper jaw length, head width, anal fin ray length, pectoral f in ray length, caudal fin ray length and eye diameter). Of the 5 characters which showed differences in all three species (body depth, head length, snout length, upper jaw length and pectoral fin ray length), females had higher values than males only in body depth, whereas males values in all other characters. The upper jaw length was the most distinctive sexually dimorphic feature, the upper jaw extended beyond the posterior margin of the eyes (p<0.001) in males but not in females.

• Korean Journal of Poultry Science
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• v.34 no.2
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• pp.151-156
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• 2007

The Function of the calcium sensing receptor (CaSR) is to control calcium levels by altering PTH (parathyroid hormone) secretion and renal calcium resorption. The influence of calcium on the basal and stimulated release of several hormones from chicken pituitary glands has been determined in vitro. The objective of this study was to identify SNP in chicken CaSR gene and to investigate the effect of the SNP on economic traits. The sequencing analysis method was used to identify nucleotide polymorphisms within chicken CaSR gene. This study identified SNP at position 1949 bp(Genebank accession No : XM_416491) in the exon 1. The SNP changed the amino acid to alanine(GCC) from serine(TCC). This SNP showed three genotypes, AA, AS and SS by digestion with the restriction enzyme NcoⅠ using the PCR-RFLP method. The A963S showed significant effect only on the first lay day (P<0.05) in Leghorn population. Leghorn with the genotype AA had significantly faster the first lay day(137.6) than the genotype AS(143.0, P<0.05). Also, the A963S showed significant effect only on the first lay day(P<0.05) and mean of egg weight(P<0.05) in KNC population. KNC with the genotypes AA ans AS had significantly faster the first lay day (151.0 and 152.6, respectively) than the genotype SS(159.4, P<0.05). And the genotypes SS had significantly heavier the mean of egg weight(50.4 kg, P<0.05) than the genotype AA ans AS (47.5 and 47.8 kg, respectively). According to result of this study, an a allele of the A963S was found to have a significant effect on the first lay day. It will be possible to use this SNP marker on selecting chicken to improve the first lay day.

• Journal of Sericultural and Entomological Science
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• v.46 no.2
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• pp.54-57
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• 2004

A new silkworm variety "Buhungjam" for autumn rearing season is $F_1$ hybrid between Jam149, a japanese race bred from introduction breeding and Jam150, a chinese race from introduction breeding. Jam149, japanese parent of the "Buhungjam" showed high GCA in pupation percentage, cocoon shell percentage and filament length and Jam15, chinese parent showed high GCA in cocoon shell percentage and cocoon filament length. In the local adaptability test performed at 8 local areas in spring of 2003, Buhungjam records 7% higher in cocoon yield and 8% in raw silk yield than the check variety, Daesungjam.

• Korean Journal of Agricultural Science
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• v.17 no.1
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• pp.26-33
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• 1990

This study was conducted to estimate heritabilities and genetic correlations of economic traits and body conformations in Korean Native Ogol fowl. The data analyzed were the record of 450 pullets bred from 150 dams and 20 sires of the fowl obtained from June 18, 1987 to February 6, 1989. The results of this study were summarized as follows ; The estimates of heritability for body weight, egg weight and shank length from combined variance components were relatively high, ranging from 0.415 to 0.558, while those for age at first egg, egg production and breast width were moderate, ranging from 0.301 to 0.334. For genetic correlations, body weights were negatively correlated with egg production, ranging from -0.552 to -0.309, while positive correlations were found between body weight and egg weight, ranging from 0.285 to 0.380. Also, egg production was negatively correlated with egg weight, ranging from -0.383 to -0.381. The shank length showed very high positive correlations with the body weights and age at 1st egg, ranging from 0.328 to 0.843. Hence the improvement in shank length will increase the body weight as well as the age at 1st egg.