• Title/Summary/Keyword: 사이중심도

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Expression of Secretion-dedicated Srb Homologue and Antifungal Activity of Bacillus lentimorbus WJ5 (Bacillus lentimorbus WJ5의 분비 전용 Srb Homologue 발현과 항진균 활성)

  • 장유신;이영근;김재성;조규성;장병일
    • Korean Journal of Microbiology
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    • v.39 no.3
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    • pp.135-140
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    • 2003
  • Bacillus sp. secretes high levels of extracellular enzymes into the culture medium. The signal recognition particle (SRP) and the SRP receptor play a central role in targeting pre secretory proteins to the translocase. By the analysis of the DNA microarray of B. lentimorbus WJ5, it was detected that WJ5m12, antifungal activity deficient mutant induced by gamma radiation, had a down-regulated expression of the SRP receptor gene (B. subtitis srb homologue, srbL). To determine the relationship of SRP receptor to antifungal activity, srbL of B. lentimorbus WJ5 was amplified by PCR and ligated into pQE30 vector, and then transferred into WJ5m12. The transformant, WJ5m12::srbL, recovered the antifungal activity. From the 2-DE analysis, the several presecretory proteins accumulated in the mutant cell and decreased to a level of the wild type in WJ5m12::srbL. It seems that the srbL could play an important role in the secretion of the antifungal activity related proteins of B. lentimorbus WJ5.

Performance Evaluation of Disk Scheduling Schemes in a VOD System (VOD 시스템에서의 디스크 스케줄링 기법의 성능 평가)

  • Jeon, Yong-Hee;Lee, Sang-Hag
    • The Transactions of the Korea Information Processing Society
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    • v.7 no.11
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    • pp.3519-3533
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    • 2000
  • In a VOD(Video-On-Demand) system, I/O performance and storage requirements are generally more important than computing capability, Due to the development of CPU tecnology, the gap between the performance of processors and desk speed are widening. Therefore, the disk system typically becomes the bohleneck ina VOD system. In order to allevte those effects from physical constramts, disk array system is used. The I/O performance of disk arrays can be improved by the capability of processors, I/O schefuling polcy, the number of member disks in the array, disk block size, block placemet method etc. In this paper, we considered the disk scheduling schemes including EDF( Earliest Deadine First), SCAN, SGAN-EDF, Round-robm. GSS(Grouped Sweeping scheme), and C-SCAN, LOOK, C-LOOK which are variations of SCAN Mamly focusing on those disk scheduling schemes, we performed the simulation in order to compare and eview the perionmance of disk arrays considening seveial related parameters under diverse environments and analyzed the effects of those parameters to the performance.

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An Innovative Public Project Process Management For the Government Owner (발주자 입장에서 바라본 혁신적인 관급공사 관리 프로세스 제시를 위한 기초 연구)

  • Kim, Sung-Won;Kim, Dea-Young
    • Proceedings of the Korean Institute Of Construction Engineering and Management
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    • 2008.11a
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    • pp.557-562
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    • 2008
  • In Korean construction industry, a public sector's ordering volume has been constantly increased after 2000. But, because a cost, schedule and quality control generally depends on a result-centric management form and a transparent management is not done during the construction in the Korean public business, it is recognized that this causes problems including an increase of cost and schedule, incomplete construction, a difficulty to find the problem in advance to establish measures, and an unclear locus of responsibility of constructor, supervisor and designer. Accordingly, this study is intended to derive the problems and the limit of management by identifying and analyzing the management state of the present government construction client for the client organization of public sector's government construction. Also, the study is intended to contribute to the client's efficient and actual project management by presenting the model of supplementing or improving the derived problem and limits to the client's organization.

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The Effect of Influencer Factors in Personal Media Contents on Purchase Intention (1인 미디어 콘텐츠의 인플루언서 요인이 상품 구매 의도에 미치는 영향: 브랜드 진정성의 매개효과를 중심으로)

  • Yu, Ines Hyunju;Kim, Hun
    • The Journal of the Korea Contents Association
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    • v.20 no.1
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    • pp.45-59
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    • 2020
  • This study is to examine the effects of personal media influencer's source credibility (attractiveness, trustworthiness, expertise) and content authenticity on brand authenticity and product purchase intention. An online survey was conducted, on which the respondents were asked to watch the embedded contents of the influencer provided on YouTube before answering the questionnaire. The results of the regression analysis showed that both influencer expertise and content authenticity had a positive effect on brand authenticity. Moreover, brand authenticity had a positive effect on purchase intention and had a mediating effect between purchase intention and content authenticity. The findings are meaningful in that they not only analyze empirical advertising effects of personal media and media contents but also expand the scope of research on authenticity. This study also provides strategic implications for personal media influencers and companies which want to collaborate with influencers.

Impact of Job Stress and Work-Family Conflict among Tollgate Female Workers on Turnover Intention (톨게이트 여성근로자들의 직무스트레스가 이직의도에 미치는 영향 :직장-가정갈등의 매개효과를 중심으로)

  • Choi, Soo-Chan;Lee, Eun-Hye;Lee, So-Min;Cho, Young-Lim
    • The Journal of the Korea Contents Association
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    • v.18 no.1
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    • pp.561-572
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    • 2018
  • Female tollgate workers are known to be the most highly exposed to job stress in the service industry. However, there have been few studies on female tollgate workers. This study was thus performed to verify the mediator effect of work-family conflicts in the relationship between job stress and turnover intention of female tollgate workers. A survey was carried out in May, 2016, and the final data were obtained from 183 female tollgate workers by quota sampling. The major result was that their job stress significantly influenced on turnover intention, and work-family conflicts between the two variables were partially mediated. On the basis of the outcomes, first, further studies are needed to develop customized supporting programs for the female tollgate workers; second, internal efforts, such as psychological and social support programs such as Employee Assistance Programs (EAPs), should be launched for the workers at the enterprise level; third, national attention must be paid to improve the work environments of female tollgate workers.

Implementation a Philosophy Ontology based on Knowledge of Text Contents (텍스트 내용 지식 기반의 철학 온톨로지 구축)

  • Kim Jung-Min;Choi Byoung-Il;Kim Hyoung-Joo
    • Journal of KIISE:Computing Practices and Letters
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    • v.11 no.3
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    • pp.275-283
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    • 2005
  • Ontologies are the core components of the Semantic Web and knowledge-based systems. But it is difficult to find useful ontologies in actual domains. In order to build useful domain ontology, the conceptualization of the domain knowledge by knowledge experts of the specific domain and the specification of conceptualized knowledge with formal languages by ontology designers are required. In addition, structured and detailed guidelines and methods should be provided to be shared by the development team members. However, existing ontology building methodologies define and describe the skeletal structure of the whole building process at the top-layer. We build a useful academic ontology that is based on the conceptual knowledge structure in the domain of philosophy, and propose a detailed methodology to build a text ontology based on Topic Maps. Our methodology consists of two phases, ontology modelling and ontology implementation. We implement a philosophy knowledge portal to support retrieving and navigating of the philosophy knowledge.

Creating and Managing Local Oral Records in Busan (지역 구술기록의 생산과 관리 현황 및 개선방안 - 부산지역을 중심으로 -)

  • Bae, Eunhee
    • Journal of Korean Society of Archives and Records Management
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    • v.18 no.2
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    • pp.85-108
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    • 2018
  • Oral history research in Korea began with the effort to find out the truth behind the hidden historical issues that occurred during the mid-1980s. Since then, attempts to record the major issues through contemporary Korean history orally has increased. From the late 2000s, the focus shifted to local and smaller village units. In Busan, from 2009 to 2016, 33 oral history projects from 10 government agencies were conducted. This research was carried out to investigate what kind of oral records has been produced in Busan from the said period and how they are managed. With these findings, possible solutions to preserve and utilize the oral records as the locality archives are suggested.

Determinants of Preschoolers' Self-determination: Focus on their Playfulness, Self-esteem, and Self-regulation (유아의 자기 결정력에 영향을 미치는 요인 :유아의 놀이성, 자아존중감, 자기조절력을 중심으로)

  • Park, Geun Joo;Seo, So Jung
    • Korean Journal of Childcare and Education
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    • v.9 no.5
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    • pp.489-511
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    • 2013
  • The main purpose of this study is to examine the effects of preschoolers' levels of playfulness, self-esteem and self-regulation on their self-determination. Furthermore, the mediational effects of the children's self-esteem in the relationship between their playfulness and self-determination. To meet the purposes of this study, 126 preschoolers aged 6-7 years old who attended early childhood education and care settings were sampled. Data on the preschoolers' self-esteem, self-regulation, and self-determination were assessed by their teachers, and their playfulness was observed by one of the researchers in this study. The main results of this study were as follows. The most influential variable on the young children's self-determination was found to be their levels of self-esteem. Also, the preschoolers' self-esteem mediated the effects of the playfulness on their self-determination. Implications for research and practice were discussed along with the results of this study.

The Effect of Stress on the Happiness Index of Nursing College Students : Focusing on Mediating Effects of Optimism (스트레스가 간호대학생의 행복지수에 미치는 영향 : 낙관성의 매개효과를 중심으로)

  • Kim, Eun-Young;Lim, Kyoung-Suk
    • Journal of Digital Convergence
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    • v.15 no.12
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    • pp.381-391
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    • 2017
  • The purpose of this study is to investigate the effect of optimism on stress on happiness index. The subjects were 3 and 4 grade students who had clinical practice experience among 3 nursing students at G university, and 362 questionnaires were used as research data. The data analysis used regression analysis, SPSS macro and bootstrapping methods to examine the mediating effects of optimism on college students' stress and happiness. The results showed that stress, happiness, and optimism were significantly correlated, and stress had a direct effect on college students' happiness. In other words, the higher the level of stress, the lower the happiness index of college students. The optimism was partially mediated between stress and college students' happiness index. Therefore, in order to increase the happiness index of college students, it is necessary to develop and implement a program for happiness enhancement as one of the program strategies that can enhance stress.

The Influence of Innovation-oriented Organizational Culture on Management Performance - Focusing on the Mediating Effect of Proactive Entrepreneurial Behaviour and Market Orientation - (혁신지향 조직문화가 경영성과에 미치는 영향 -진취적 기업가행동과 시장지향성의 매개효과를 중심으로-)

  • Joo, Cheol-Keun
    • Journal of Digital Convergence
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    • v.18 no.8
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    • pp.119-131
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    • 2020
  • The purpose of this study is to examine the mediated effects of entrepreneurship and market orientation on organizational culture and management performance as the cause of the reversal phenomenon of startups defeating traditional giants. Through the research method of bootstrapping proposed by Hayes (2013), the total effect, direct effect and indirect effect analysis were conducted. As a result, there was no direct impact relationship between organizational culture and management performance, but it was confirmed that it had a positive impact on management performance by mediating both entrepreneurial behavior and market orientation in the relationship between organizational culture and management performance. This study is meaningful in that it is linked to existing theories such as entrepreneurship and market orientation and it extends the scope of competency targets required for business performance to an organizational culture. Companies need to create an innovation-oriented organizational culture for management performance and value creation, and they have to open up their culture with entrepreneurial behavior and market orientation by breaking away from the inertia of organization.