• Title/Summary/Keyword: 사육

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Transmission modes of Pneumocystis carinii among rats observed by karyotype analysis (핵형 추적에 의한 카리니주폐포자충의 전파 양상 확인)

  • 홍성태;류진숙
    • Parasites, Hosts and Diseases
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    • v.30 no.4
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    • pp.283-288
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    • 1992
  • To observe the transmission patterns of karyotype of Pneumocystis carinii (Pc) by rat colonies, three strains of rats, Sprague-Dawlcy(SD), Wistar(W) and Fisher (F) from various animal vendors, were suppressed of their immunity by injection of methyl prednisolone. They were kept for 5 to 13 weeks in 3 different animal rooms, A, B, and C. The purified organisms were prepared in low melting point agarose gel by embedded Iysis method for pulsed field gel electrophoresis. Field inversion gel electrophoresis showed 2 patterns of the kart·otype of Pc. The rooms A and C contained SD rats from the source p, and also the room A was used for F and W rats. However, Pc from all of the SD and F rats in the room A showed same karyotypes, the pattern I. The SD rats from difFerent vendors, M and 5, were reared in the room B, and shared the same Pc karyotypes, the pattern II . The rats of W strain were from the vendor M, and immune-suppressed in the animal room A. Five weeks after the expe- riment, the Pc showed the karyotype pattern II but the pattern became mixed with the type I after 7 to 8 weeks. The Bindings revealed that the animals born and reared in the same animal quarter harbored Pc with same karyotypes. If the animals were kept under immune-suppression in the same room with heavily infected hosts, they could be infected by Pc from their neighbors. The present experimental findings suggest that Pc is transmitted among rats through the air.

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Rearing and Innoculation Condition of Nd hybrid Silkworms for Paecilomyces tenuipes Production (Paecilomyces tenuipes 생산을 위한 나용교잡종 누에 사육 및 균 접종 조건)

  • Choi, Ji-Young;Kim, Sam-Eun;Kim, Jong-Gill;Nam, Sung-Hee;Yoon, Hyung-Joo;Choi, Young-Cheol
    • Journal of Sericultural and Entomological Science
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    • v.48 no.1
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    • pp.16-20
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    • 2006
  • To shorten the labor cost for the production of the fungus, Paecilomyces tenuipies, uncocooning mutant hybrid silkworms as a host was used. The yield of P. tenuipies was reduced when Nd (uncocooning mutant) was used as a host because Nd was smaller than the hybrid Baegokjam that has been used as a typical host. To increase the host weight, Nd was crossed with Jam l23 or Jam 124, and resulted in not only greater weighter but also higher yield of P. tenuipies. The optimum temperature for rearing Nd hybrid silkworms was $3^{\circ}C$ higher than standard temperature of rearing silkworms to increase the naked pupation rate. The optimum time for the infection of P. tenuipies was 12 h at $28.5^{\circ}C$ and 95% R.H., when $1{\times}10^7spores/ml$ of P. tenuipies were sprayed on the surface of the silkworm. The conditions were applicable to both spring-rearing and autumn-rearing.

Development of the Insect Smart Farm System for Controlling the Environment of Protaetia brevitarsis seulensis

  • Rho, Si-Young;Won, Jin-Ho;Lee, Jae-Su;Baek, Jeong-Hyun;Lee, Hyun-Dong;Kwak, Kang-Su
    • Journal of the Korea Society of Computer and Information
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    • v.24 no.12
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    • pp.135-141
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    • 2019
  • In this study, the "Insect Smart Farm Air Conditioning System" is designed and proposed for the control of breeding environment of Protaetia brevitarsis seulensis larvae. The proposed "Insect Smart Farm Air Conditioning System" separates the breeding room from the air conditioning room. It is a system that creates an environment optimized for breeding and distributes it into a breeding room. When controlling the environment through air-conditioning and humidifiers in insect farms, temperature and humidity vary from part of the breeding room to part. The solution to the problem can be suggested as a solution to the difficulty of producing white-spotted flower mounds of uniform size and weight when selling edible insects. By using the "Insect Smart Farm Air Conditioning System," the temperature difference can be reduced by 6℃ and the humidity difference by 24.7% compared to the environmental control of existing insect farms. The temperature and humidity of different parts of the breeding room were improved. Provide the optimal environment of Protaetia brevitarsis seulensis larvae at all times and ensure uniform CO2 concentration. It can be expected to increase output through annual production and increase income for insect farmers. The proposed "Insecting Smart Farm Air Conditioning System" also controls the set temperature, humidity and CO2. Environmental control of the breeding of other edible insects and the reproduction of mushrooms that require environmental control in breeding or breeding will also be possible.

Studies on the Growth and Molting of the Tiger Crab, Orithyia sinica (Linnaeus) (범게, Orithyia sinica (Linnaeus)의 성장과 탈피에 관한 연구)

  • KIM Young-Ho;CHUNG Sang-Chul
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.23 no.2
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    • pp.93-108
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    • 1990
  • Complete larval development Orithyia sinica (Linnaeus) is composed of three zoeal ins-tars and megalopa. Survival of instals was higher in $20^{\circ}C$ than in ambient temperature. Intermolt period generally increased with the increase of number of instals, but a notable decrease was observed from Crab-5 to prepuerty molt instals(Crab-7). Zoeas and me-galopa reared at higher temperature regime were samller than those reared at lower temperature. No temperature effect was shown in the size of crab instals. Growth factors fluctuate with the increase of number of instals, but there was an overall trend of net decrease. Growth factor was $35.65\~41.4\%$ for zoeal instals, and they were $18.00\~30.91\%$ for crab instals.

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Effect of Animal-Welfare Environment on the Metabolomic Properties of Breast and Thigh Meat from Two Broiler Strains (동물복지 사육환경이 두 육계 품종의 가슴육 및 다리육의 대사체학적 특성에 미치는 효과)

  • Lee, Dongheon;Jung, Jong Hyun;Jo, Cheorun
    • Korean Journal of Poultry Science
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    • v.48 no.4
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    • pp.239-253
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    • 2021
  • This study investigates the metabolomic changes in breast and thigh meat from Cobb and Ross 308 chickens regarding the rearing environment. One-day-old Cobb and Ross broilers were raised for 35 days in conventional and animal welfare farms with, amongst others, different floor sizes, stock densities, and ammonia concentrations. One-dimensional 1H nuclear magnetic resonance, orthogonal partial least squares-discriminant analysis (OPLS-DA), and pathway analyses were performed to analyze the metabolomic properties of broiler meat. For breast meat, only those from the Ross strain could be separated according to the environment in the OPLS-DA plot. Ross breast meat from animal welfare farms showed significantly higher acetate, anserine, creatine, and inosine monophosphate content than those from conventional farms (P<0.05). In contrast, for thigh meat, the Cobb strain was differentiated using OPLS-DA. The contents of five metabolites, such as glucose and lactate, were higher in thigh meat from animal welfare farms; however, nine metabolites, including seven free amino acids, were lower compared to those from conventional farms (P<0.05). Pathway analysis was performed to interpret the biological changes in chicken meat based on environmental factors. The results indicated that the animal welfare environment led to significant changes in four metabolic pathways in Ross breast meat and in 20 metabolic pathways in Cobb thigh meat (P<0.05). In conclusion, the animal welfare environment could influence the metabolomic properties of Ross breast meat and Cobb thigh meat, which may affect the sensory quality of meat.