• Korean Journal of Agricultural Science
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• v.32 no.1
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• pp.53-61
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• 2005

Many bovine genetic diseases are currently unidentified in Korea because of the relatively low monitoring systems in the livestock farms. The molecular detection system using PCR-RFLP of two genetic diseases, namely Band 3 (Erythrocyte Membrane Protein Band III) and CHS (Chediak-Higashi Syndrome), have been identified in Japan and used for screening large number of cattle whether each individual has the genetic disease or not. Using the 22 unrelated Korean cattle (Hanwoo) individuals, molecular detection system based on PCR-RFLP have been investigated, which can be distinguishable carriers for the genetic diseases. Even though we could not found the causative mutations for two genetic diseases, the PCR-RFLP techniques used in this study are very valuable for the screening the genetics diseases in Korean cattle, especially for the proven or candidate bulls.

• Proceedings of the Korea Institute of Fire Science and Engineering Conference
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• 2013.11a
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• pp.143-143
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• 2013

대학, 기업 및 국 공립 출연 연구소의 연구 활동 종사자는 학술적 연구 및 신기술, 신물질의 개발을 통해 우리나라 과학기술 발전에 크게 기여하고 있다. 그러나 이와 더불어 각종 안전사고에 노출되어 있는 것도 사실이다. 즉, 실험실은 대부분의 작업장 보다 더욱 많은 화재, 폭발 등의 위험에 항상 노출되어 있다. 이는 일반적인 작업장과는 다르게 실험이 종료되면 기존 실험 장치를 철거하고 신규로 다른 실험장치가 설치되기 때문이다. 연구실험실에서 발생한 사고 원인을 분석해 보면, 실험실 기능의 불비, 설비 및 유지관리의 불충분, 실험공간의 협소, 실험 작업 안전에 대한 배려부족, 안전전문가의 부족과 교육훈련체제의 미흡 등을 지적할 수 있으며, 이와 같은 빈번한 사고발생은 우수한 연구 활동 종사자의 연구 의욕을 떨어뜨리고, 건강과 생명에 위협을 느끼게 하고 있다. 본 연구에서는 기존의 실험실 안전관리 체크리스트들을 검토하고, 안전관련 분야에서 사용되고 있는 심의 체크리스트를 조사하여, 대학 및 연구기관 등의 바이오실험실에 사용할 수 있는 표준 체크리스트를 개발하고자 한다. 현재 개발된 바이오 분야 체크리스트는 "연구실험실 공통 체크리스트"에 기반하여 바이오 분야에서 중점 평가할 항목을 심화시켜 구성하였다. 바이오 분야에서 주로 이루어지는 생물 실험 및 동물해부 실험시 안전을 위해 반드시 점검해야 할 총 8개의 세부 점검 항목을 제시하였으며, 실험동물 관리 및 사육실 출입시 안전을 위해 점검해야 할 총 11개의 세부 점검 항목을 제시하였고, 세포, 조직 배양 실험 및 유전자 조작 실험시 안전을 위해 점검해야 할 총 5개의 세부 점검 항목을 제시하였다. 또한 미생물 취급시 안전을 위해 점검해야 할 총 19개의 세부 점검 항목을 제시하였으며, 세균 취급시 안전을 위해 점검해야할 총 8개의 세부 점검 항목을 제시하였고 마지막으로, 바이러스 취급시 안전을 위해 점검해야할 총 5개의 세부 점검 항목을 제시하였다. 실험실 안전은 연구종사자의 올바른 안전의식, 연구관련 부서의 체계적 지원 및 실험실 안전 관련 법령의 완비가 뒷받침 되었을 때 비로소 가능한 일이다. 따라서, 본 연구에서 얻어진 체크 리스트가 이에 기여할 것이라 사료되며, 추후 분야별 실험실 안전관리 실태조사의 지침서로 활용되는데 목적이 있다.

• Journal of Animal Science and Technology
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• v.54 no.4
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• pp.255-265
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• 2012

The objectives of this study were to investigate MC1R genotype, coat color, and muzzle phenotype variationsin the Korean native brindle cattle (KNBC) maintaining family lines and to establish the mating system for increased brindle coat color appearance. KNBC with genotype and phenotype records were selected as experimental animals. The relationship between melanocortin 1 receptor (MC1R) genotypes, verified by PCR-RFLP, and brindle coat color appearance was determined. Fragments of the MC1R gene amplified by PCR were digested with MspI and RFLP was determined. KNBC had $E^+E^+$, $E^+e$, and ee genotypes. The $E^+e$ genotype was most common with 65%, compared to $E^+E^+$ (33.33%), or ee (1.67%). When the sire had $E^+e$ genotype and the dam had $E^+E^+$ genotype, and both of them had the whole body-brindle coat color, all of their offspring (4/4) had whole body-brindle coat color. When the sire had $E^+E^+$ genotype and the dam had $E^+e$ genotype, and both had whole body-brindle coat color, 44.44% (4/9) of the offspring had whole body-brindle coat color. The mating between the sires and dams with these two genotypes with whole body-brindle coat color may have the highest whole body-brindle coat color appearance in their offspring. Muzzle grades 3 or 4 were more common than other muzzle grades. This is the first report indicating the segregation of MC1R genotypes and the inheritance of coat color through family lines in KNBC. The mating system proposed from this study may increase the possibility of brindle coat color appearance in KNBC.

• Journal of agriculture & life science
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• v.44 no.6
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• pp.91-99
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• 2010

We used a multiplex PCR primer set composed of 11 microsatellite (MS) markers and two sexing markers for gender detection. Genomic DNA extracted from hair roots of 3,510 Hanwoo were genotyped. Based on the 11MS markers, no animals had identical genotypes(TGLA227, BM2113, TGLA53, ETF10, SPS115, TGLA122, ETH3, ETH225, BM1824 and INRA23). The expected probability of identity among genotypes of random individuals (PI), the probability of identity among genotypes from random half-sibs ($PI_{half-sibs}$) and among genotypes of random individuals, and the probability of identity among genotypes from random sibs ($PI_{sibs}$) were estimated as $1.31{\times}10^{-23}$, $2.52{\times}10^{-16}$and $1.09{\times}10^{-6}$, respectively using the API-CALC program, version 1.0. We successfully completed the genotype analysis of 3,510 Hanwoo with a 3.93% genotyping failure rate. It was revealed that extracting DNA from the hair root was a time-efficient and cost-effective method to collect specimens for DNA isolation from live animals. This method also minimized stress for the animals during specimen collection. Among the hair roots from the back, belly, upper tail and lower tail, 5~13 hair roots of the lower tail led to the best genotype analysis results. Finally, we established a 96-well-format method of DNA preparation applicable for high- throughput genotype analysis.

• Korean Journal of Environmental Biology
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• v.38 no.1
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• pp.165-178
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• 2020

This study investigated the exotic mammals introduced to Korea to present the basic data necessary for ecological risk assessment and the establishment of an alien species management database. For this purpose, a list of alien mammals introduced to Korea was established by investigating alien mammals introduced for zoo exhibits purposes, alien mammals in breeding environments traded between individuals, and alien mammals introduced into nature. A total of 163 taxa were identified as alien mammals introduced to Korea. There were 139 taxonomic groups of ornamental alien mammals, including 11 orders, 44 families, 129 species and 10 subspecies; 28 taxonomic groups of alien mammals traded between individuals, including five orders and 19 families; and eight taxonomic groups of alien mammals introduced into nature, including four orders, seven families, seven species, and one subspecies. For the effective management of alien mammals introduced to Korea, it is important to collect basic information, such as a list of all species introduced, their populations, and introduced areas. It is also necessary to apply proactive management policies according to the degree of potential risk by preemptively evaluating the ecological risks that may arise from the introduction of alien species into natural ecosystems. For the eight taxonomic groups of Capra hircus, Cervus nippon taiouanus, Felis catus, Myocastor coypus, Oryctolagus cuniculus, Ondatra zibethicus, Ovis aries, and Rattus norvegicus identified to be introduced into the natural ecosystem of Korea, the provision of proliferation control measures at the national level, continuous monitoring of changes in diffusion trends, and immediate response based on the degree of change are required.

• Journal of the Korean Society of Laryngology, Phoniatrics and Logopedics
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• v.7 no.1
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• pp.128-131
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• 1996

소아들의 음성장애의 대부분은 음성과 기능에 의한 성대의 오용 및 남용으로부터 기인한다. 그러나 음성장애에 대한 인식의 부족으로 인하여 소아의 음성장애 발견율은 극히 낮은 수준에 머므르고 있다. 치료에 있어서도 행동의 통제가 그다지 쉽지 않기 때문에 많은 어려움이 따른다. 본 연구는 소아의 음성장애의 발생경위와 문제의 설명과 차팅에 의한 남용의 감소에 초점을 둔 치료사례이다. 5세 아동의 심한 음성장애로 의뢰되어졌다. 음성증후는 목쉰소리, 기식화된 소리, 일시적인 발성일탈(phonation break) 등이 두드러졌다. 이비인후과 전문의의 스트로보스코프(stroboscope) 검진결과 초기 양측성 성대결절이 성대 앞쪽으로부터 1/3 지점에서 발견되었으며 비지피치(Visi-Pitch)로 측정된 퍼터베이션(perturbation 수치가 16.8이었다. 사례사 조사 과정에서 아동이 활동적이며 자주 고함을 지르고 자동차놀이를 주로하며 많은 시간을 보낸다는 것이 밝혀졌다. 중재 프로그램은 우선 문제를 설명하고 인식시키는 것과 고함을 지르거나 자동차놀이를 하면서 자동차 소음을 흉내내는 등의 음성남용 회수를 감소시키는 것에 초점을 두었다 17회의 치료로 성대결절이 감소되었고 퍼터베이션은 3.8로 저하되었으며 아동 스스로 음성남용을 통제할 수 있다는 임상적 판단하에 치료를 종결하였다. 1개월 후 추후 검사에서 남용적인 습관이 여전히 나타나지 않았으며 음성상태도 양호하였다. 소아 음성장애의 치료는 소아가 이해할 수 있는 용어로 문제의 인식을 확실히 시키고 행동을 체계적으로 통제할 수 있는 치료프로그램을 실행시키는 임상가의 자질이 대단히 중요하다. 그러나 그에 못지 않게, 많은 시간을 아동과 함께 보내는 부모나 교사의 협력이 치료의 성패를 좌우할 수 있다.조구와 처리구간에 차이가 없었다. 그러나 유지방 함량의 경우 대조구가 3.22%, 처리구가 3.37%로 처리구가 0.15% point 증가했으나 유의차는 없었다. Fan ＋ sprinkler가 설치된 시설에서 사육된 젖소에서 생산된 우유내 체세포수는 대조구에 비해 26.63%가 감소되었으나 유의차는 발견할 수 없었다. 이상의 시험 결과에서 볼 때 fan ＋ sprinkler를 이용한 방서 방법은 고온 stress를 완화시켜 유생산성의 증가에 기여할 수 있는 하나의 방법이라고 본다.을 알 수가 있었다.없다. 본 논문의 대상은 바로 이러한 합성 동사성명사의 논항구조와 동사성명사에 의해 하위범주화된 논항들의 문법적 실현양상이다..그 결과 심리적 특성과 사용자 수용 특성은 아바타 수용도에 부분적으로 영향을 미친다는 것 결과가 나타났다.웨어 프로세스 평가와 개선 모델의 개발을 위한 기초적인 자료를 제공할 것으로 예상된다 또한, 본 연구 결과는, 우리나라 소프트웨어 조직들이 실제로 무엇을 필요로 하는지를 밝힘으로써, 우리나라의 소프트웨어 산업을 육성하기 위한 실효성 있는 정책 입안을 위한 기초 자료를 제공할 것으로 예상된다.다.를 검증하려고 한다. 협력체계 확립, ${\circled}3$ 전문인력 확보 및 인력구성 조정, 그리고 ${\circled}4$ 방문보건사업의 강화 등이다., 대사(代謝)와 관계(關係)있음을 시사(示唆)해 주고 있다.ble nutrient (TDN) was highest in booting stage (59.7%); however no significant difference was found among other stages. The concentrations of Ca and P were not

• Korean Journal of Poultry Science
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• v.35 no.3
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• pp.291-302
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• 2008

To acquire essentially necessary basic data to establish feeding system by verifying appropriate dietary energy and protein level for the growth of commercial slow growing broiler chicks within the country, two experiments were conducted for 5 weeks. One day old, 1,404 male and female broiler chicks were used for the experiments, and 26 chicks were placed at each pen. The energy level of feed was maintained about 3,000 or 3,100 kg/kcal for whole breeding period of 5 weeks, and protein content was adjusted about 20, 21, and 22% during the first two weeks and the content was adjusted to 18, 19, 20, 21, and 22% from the 3 to 5 weeks old of the experiment. The categories of body weight and feed intake amount were monitored to calculate the productivity and blood sampling was conducted for the analysis at the end of each experiment. Experiment 1:Although the productivity by the ME content difference during $0{\sim}2$ weeks did not have significant difference and the body weight increase by the difference of CP content and feed intake amount did not have much difference, the feed requirement rate was statistically improved in CP 21 and 22% treatment groups compared to the CP 20% group (P<0.05). The feed ME 3,100 kcal/kg treated group during $3{\sim}5$ weeks after starting the experiment revealed to show improved feed requirement rate (P<0.05). Within the period of experiment, the CP 22% treated group resulted to show significant body weight increase compared to the groups treated with low levels of CP (P<0.05) and the feed requirement rate was improved in high CP treated group compared to low CP treated groups, but the feed intake amount did not show significant difference between treated groups. During the experiment period, the body weight increase and feed requirement rate revealed to interact between ME and CP (P<0.05). During the whole experiment period of the 5 weeks, the feed requirement rate was improved in ME 3,100 kcal/kg treated group than the groups treated with ME 3,000 kcal/kg, and the CP (20) 18% treatment groups resulted to show higher values than other treatment groups (P<0.05). Body weight increase was high in CP (22) 22% treated groups than those of CP (21) 21% and (20) 18% treated groups, and the interaction between ME and CP was found at body weight increase and feed requirement rate (P<0.05). Although blood albumin and total cholesterol levels were elevated in ME 3,100 kcal/kg treated group than ME 3,000 kcal/kg treated group, but neutral fat content was reduced (P<0.05). On the other hand, the total cholesterol content was increased in CP (22) 21% treated group than CP (22) 20% and CP (20) 18% treated groups (P<0.05). Experiment 2: The body weight increase in 0-2 weeks was higher in ME 3,100 kcal/kg treated group than ME 3,000 kcal/kg treated group, and it was highly improved in CP 22% treated group than CP 20% treated group by showing the interaction between CP and ME (P<0.05). The significant improvement of feed requirement rate was observed in CP 21% and 22% treated groups compared to CP 20% treated group (P<0.05). The productivity between the growth period from 3 to 5 weeks of age and whole growth period resulted to show no significant difference. Although no difference was observed in blood composition between treated groups, the interaction of ME and CP on cholesterol content was accepted at the range of P<0.05). Therefore, it is considered that the appropriate dietary protein level within feed for the physiology of growing broiler chicks was 22% or more for the first two weeks and protein level of 21% or 20% from 3 to 5 weeks old for the maximization of productivity. Even if the energy level within feed had some partial effects on the productivity, but did not show consistency. So, further experiments needto be conducted by differentiating the energy level.

• Korean Journal of Poultry Science
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• v.35 no.4
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• pp.399-405
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• 2009

Two experiments were conducted to establish the basic data of feeding system in cross-bred Korean native chicks. A total of seven hundred twenty chicks were replaced the 36 floor pen for the first five weeks with $3{\times}3$ factorial design in Experiment 1. Four hundred eighty of five weeks old chicks were raised from six to ten weeks of age with $3{\times}2$ factorial design in Experiment 2. Dietary ME and CP were formulated to contain 3,000, 3,050, 3,100 kcal/kg and 21, 22, 23%, respectively in Experiment 1 and 3,050, 3,100, 3,150 kcal/kg and 18, 19% in Experiment 2. Weight gain, feed intake were measured and calculated the feed conversion. Blood were collected and analyzed at the end of experiments. In Experiment 1, weight gain showed significantly higher in 3,050, 3,100 kcal/kg treatments than 3,000 kcal/kg treatment (P<0.05), but was not different in CP treatments. Feed intake was statistically high in 3,000 kcal/kg treatment compared with 3,050 and 3,100 kcal/kg ones (P<0.05), and more increased in 21% CP treatments compared to that of 22 and 23 CP treatment (P<0.05). Feed conversion of birds fed 3,050 and 3,100 kcal/kg diet showed much lower than 3,000 kcal/kg treatments (P<0.05). FCR was signicantly improved (P<0.05) in chicks fed diets containing 21 and 22% CP as compared to that fed 20% CP. Blood protein, glucose, and total cholesterol tended to increase in high energy and diet treatments. Blood HDL was increased as dietary energy increased, whereas LDL increased in low CP treatments. In Experiment 2, weight gain was not consistent between treatments, but more increased in 18% CP treatments compared to that of 19% CP treatment from six to ten weeks old in cross bred chicks (P<0.05). Feed intake was similar to the result of weight gain, but more increased in 19% CP treatment than 18% CP treatment (P<0.05). There were no statistically difference in FCR, but seemed to improve as dietary ME increased. Blood total protein and glucose increased as dietary CP was high, but triglyceride and HDL increased in high versus low ME (P<0.05). The results of these experiments suggested that optimum dietary ME and CP, were 3,050, 3,150kcal/kg and 22, 19% for the first five weeks and second one, respectively.

• Journal of Animal Environmental Science
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• v.17 no.2
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• pp.81-92
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• 2011

When we bury the infected poultry into the ground, we have many problems such as the difficulty of making sufficient area for burying, environmental contamination by the leachate, unpleasant ordor. Also, in case of burning the carcass of the infected poultry, there are some problems such as high cost, dust, unpleasant odor, etc. It could cause environmental contamination which many peoples and environmental organization complains about. In this study, we develop a treating system which treats the infected poultry carcass in a environmental method preventing the environment contamination. This system is composed of many processes. The euthanasia system uses rigid vinyl to trap and to do a euthanasia the infected poultry with lethal gas, carbon dioxide. And then, with the tractor attached grappler infected poultry carcass could be put into the carcass treating system. The euthanasia system uses rigid vinyl to trap the infected birds and to confine lethal gas, carbon dioxide. Infected poultry carcass are moved to carcass disposal system by collecting device which is attached at tractor. The carcass treatment system (capacity of disposal : 6.3 $m^3$) is installed on a truck and do one pass work, which is input, crush, stir, sterilize, and discharge treated carcass. 1,000 chickens was killed within 9.7min by $CO_2$ (300L/min) in the tent (10 $m^3$). The collecting device could carry 142 chickens at a time, and the movable carcass treatment system could sterilize 2 tons carcass per hour (at one time). This treatment systems was eco-friendly because it reduced the volume of carcass by 31.9% with no wastewater generation.

• Journal of The Korean Society of Grassland and Forage Science
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• v.40 no.2
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• pp.111-116
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• 2020

This study was conducted to determine the maintenance energy requirements of growing goats in order to establish an appropriate energy benefit system, to reduce feed costs and improve livestock productivity of goat farmers, and to present basic data for detailed specifications afterwards. This experiment was conducted as a group specification test for a total of 3 months, with 32 goats of three months age and conducted by four treatments with different energy levels (T1: NRC+0%, T2: NRC+10%, T3: NRC+20%, and T4: NRC+30%). The average daily gain was the highest in the treated with NRC + 10% of the energy level of the experimental diet, and the feed conversion ratio was in the range of 6.3 g to 7.3 g in the group feeding experiment. Although there was no significant difference in digestibility between treatments, the digestibility of dry matter, crude protein, and crude fat was higher in T2 treated with NRC + 10% than the other treatments. Through the regression equation of the values of MEI and ADG obtained through the experiment (Y=0.5439X+111.51, R²=0.712), the maintenance energy requirement of the goat in the growing period was estimated to be 111.51 kcal/kgBW^0.75.