• Title/Summary/Keyword: 사상균

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Antifungal and Antioxidant Activities of Volatile Organic Compounds Generated During the Drying Process of Chamaecyparis obtuse (편백나무 건조 중 발생하는 휘발성 유기화합물의 항진균 및 항산화 활성)

  • Seo, Young-Jun;Lee, Jae-Won
    • Korean Journal of Microbiology
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    • v.48 no.4
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    • pp.305-308
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    • 2012
  • In this study, we evaluated antifungal and antioxidant activities of condensed liquid of volatile organic compounds generated during the drying process of Chamaecyparis obtuse. Condensed liquid of volatile organic compounds were extracted with hexane and ethyl acetate, respectively. The extracts were used for antifungal activity at range from 100 to 500 mg/L Antifungal activity of extracts increased as the extracts concentration increased, the activity against tested dermatophytes was high at 500 mg/L. Among extract solvents, antifungal activity was higher at hexane extract than the ethyl acetate. Especially, the antifungal activity against Trichophyton rubrum was higher than other tested dermatophytes. Antioxidant activity was 90% above 25 mg/L regardless of the extractive solvents, while the hexane extract showed high antioxidant activity below 25 mg/L. Major compounds of hexane extract were torreyol, alpha-cadinol, and tau-cadinol. However, major compounds of ethyl acetate extract were alpha-amorphene, alpha-cadinol, and gamma-cadinene.

Characteristics of Antimicrobial Activities for the Human Pathogenic Microorganism by Extracts from Korean Mushrooms (버섯 추출물이 인체 병원성 균에 미치는 항균활성의 특성)

  • Kim, Sung-Tae;Lee, Kang-Hyeob;Min, Tae-Jin
    • The Korean Journal of Mycology
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    • v.31 no.2
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    • pp.67-76
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    • 2003
  • This study was performed to screen antimicrobial activities of 198 extracts from 66 Korean mushrooms against 19 human pathogenic microorganisms using paper disc method. Mushrooms were extracted with petroleum ether 80% ethanol and distilled water in that order Among the extracts with antimicrobial activities, 1 water extract of Amanita virgineoides, 8 ethanolic extracts including Amanita and 1 petroleum ether extrac of Psathyrella hydrophila were highly active against fungi, respectively. In addition to, 24 extracts including Amanita pseudoporphyria, Amanita spissacea, 3 extaracts including Paxillus curtisii were highly active against Gram negative and positive bacteria, respectively.

Hyphomycetes from Korean Soil. II. (한국 토괴중의 사상균에 관한 연구 II)

  • Kyung Hee MIN;Hong Soon Woo;Tatsuo YOKOYAMA
    • Korean Journal of Microbiology
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    • v.18 no.2
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    • pp.104-114
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    • 1980
  • Fourteen species of the hyphomyceteous fungi isolated from Korean soils are described and illustrated. Among these, one species has teleomorphic state and is identified as Emericella nidulans var. nidulans, similar to Emericella spectabilis with the exception of size of the conidiophores as well as color and the arrangement of the hulle cells. Four species of hyphomyceteous fungi. Chrysosporium pannorum, Doratomyces microsporus, Trichoderma Roningii, T. viride, are reported here for the first time in Korea.

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섬유제품의 항균방취가공

  • 이명섭
    • The Microorganisms and Industry
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    • v.15 no.1
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    • pp.16-17
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    • 1989
  • 우리들의 주변환경에는 어느 곳에나 여러가지 미생물이 무수하게 존재하며 이들 미생물들은 그 환경조건이 이들의 서식에 적합하기만 하면 증식한다. 이들 미생물들 가운데서 섬유재료에 영향을 주는 것으로는 주로 일부의 세균과 곰팡이(사상균)류가 있다. 섬유에 영향을 주는 작용을 대별하면 1) 섬유재료의 파괴(부패작용), 2) 섬유제품의 착색오염, 3) 섬유중에서 증식되는 병원균에 의한 질병 유발, 4) 악취발생 등이라고 할 수 있다. 우리의 환경은 각종 미생물에 오염되기 쉬운 상태에 있으므로, 섬유재료의 파괴를 방지하고 될 수 있는대로 건강하고 쾌적한 의생활을 영위하려는 목적으로, 세균과 곰팡이의 증식을 억제 또는 저지 할 수 있는 항균제를 섬유제품에 처리하게 되었다.

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Soil Microbial Diversity of Paddy Fields in Korea (논 토양 서식 미생물의 다양성에 관한 연구)

  • Suh, Jang-Sun;Shin, Jae-Sung
    • Korean Journal of Soil Science and Fertilizer
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    • v.30 no.2
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    • pp.200-207
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    • 1997
  • In order to evaluate the soil microbiological characteristics of paddy fields in Korea, surface soils were sampled from 63 sites in different agroclimatic zones before submersion of the fields. The distribution of microorganisms and the microbial diversity indices were examined. Soil microbial populations were generally higher in southern area than in northern area. The colony forming units(cfus) of fluorescence Pseudomonas sp. showed the greatest regional differences, among the microbes investigated. On the topographical differences, the cfus of aerobic bacteria, fluorescence Pseudomonas sp. and Azotobacter sp. maintained high level in coastal plains; and on the sail textural difference, fungus was the highest in clay soil, but Bacillus sp., Azotobacter sp and denitrifiers were the highest in silty clay loam soil at 0.05 probability level based on the multiple range test. The numbers of ammonium oxidizers and Azotobacter sp. were increased with soil pH. Microbial diversity indices of paddy fields which calculated from the percentages of Bacillus sp. fluorescence Pseudomonas sp. Azotobacter sp. denitrifiers, ammonium oxidizers, nitrite oxidizers, actinomycetes and fungus to these total microbial numbers were between 0.109 and 0.661. On the soil textures, the microbial diversity indices of sandy, sandy loam, silty clay loam, clay loam and clay soil were 0.443, 0.427, 0.414, 0.405 and 0.362 respectively.

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Antifungal Effect and activity spectrum of crude antifungal proteins from hemolymph of larvae of Tenebrio molitor in Korea (한국산 갈색거저리로부터 분리된 항진균단백질의 항균효과와 그 작용 범위)

  • Chung, Seung-Jo;Lee, Young-Hoon;Chung, Jae-Hoon;Lee, Bok-Ruel;Han, Dong-Min
    • The Korean Journal of Mycology
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    • v.23 no.3 s.74
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    • pp.232-237
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    • 1995
  • Antifungal protein from the hemolymph of larvae of Tenebrio molitor in Korea was partially purified by $C_{18}$ open column chromatography and assayed for the activity spectrum using 3 kinds of yeast and 4 kinds of filamentous fungi. The crude antifungal protein showed static activity for a broad range of fungal species. Weak cidal effects were observed in growing yeast type cells, including Candida and Saccharomyces. The affected cells were changed from ovoid to swollen and spherical form in shape. For filamentous fungi including Aspergillus and Fusarium, the crude antifungal protein affected the spore germination and the hyphal growth but not the viability significantly.

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Development of Soil Conditioner Using Wood Decay Fungus and Forest Residue (임지폐잔재와 목재부후균을 이용한 토양개량제의 개발)

  • Min, Kyeong-Heui;Kim, Jae-Young;Yoon, Young-Han;Park, Heon
    • Journal of the Korean Wood Science and Technology
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    • v.34 no.1
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    • pp.61-67
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    • 2006
  • The purpose of this study was to develop soil conditioner using wood decay fungus. The effect of soil conditioner treated with SJ-28 fungus was tested by lettuce cultivation. Cultivating efficiency was investigated by the yield of lettuce. In the plot of soil conditioner treated with SJ-28 fungus (FPG) showed weight of lettuce leaves 4425 g of total yield which was greater than 2434 g shown in the control. Also the numbers of actinomyces and fungi were increased in the plot of soil conditioner treated with SJ-28 fungus (FPG), beneficial for soil. The number of bacteria showed decrease in FPG plot. FPG showed possibility as a soil conditioner.

Antifungal Activity of Extracts from Black Heartwood of Cryptomeria japonica against Dermatophytes (삼나무 흑심재 추출물의 피부사상균에 대한 항진균 활성)

  • Lee, Su-Yeon;Gwak, Ki-Seob;Lee, Jun-Jae;Yeo, Hwanmyeong;Choi, In-Gyu
    • Journal of the Korean Wood Science and Technology
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    • v.37 no.3
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    • pp.265-273
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    • 2009
  • The aim of this study was to investigate the antifungal activitiy of extracts from different parts of Cryptomeria japonica against Trichophyton mentagrophytes, Trichophyton rubrum and Microsporum gypseum. Disc diffusion and agar dilution methods were used for determining the antifungal activity of extracts, and their major constituents were analyzed by GC/MS. Black heartwood extract among the extracts showed the highest antifungal activity against dermatophytes. TLC was used for fractionating the effective fraction from the black heartwood extract with n-hexane and chloroform as developing solvents, and then antifungal activity of each fraction was examined against dermatophytes. As a result, seventh fraction showed the highest antifungal activity among nine fractions. The major constituent of the seventh fraction determined by GC/MS was expected to be sesquiterpene, damascone with 2 more carbon atoms. The other constituents were also identified as elemol, eudesmol and hinokione.

Isolation and Characteristics of an Amylase-producing Fungus for Saccharifying Food Wastes (음식물쓰레기 당화를 위한 Amylase 생산균의 분리 및 특성조사)

  • Li, Hong-Xian;Kim, Seong-Jun
    • KSBB Journal
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    • v.22 no.2
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    • pp.114-118
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    • 2007
  • In this study, an amylase-producing fungus, strain 15 was isolated from soil in order to saccharify food wastes with cellulolytic and amylolytic enzymes. The amylase production cultures were performed in Mandel's medium with 1% rice straw and 1% paper wastes as carbon sources. The strain produced various cellulolytic (FPase 0.25, xylanase 20.09, CMCase 3.15 U/mL-supernatant) and amylolytic ($\alpha$-amylase 1.20, gluco-amylase 0.70, $\beta$-amylase 2.40 U/mL-supernatant) enzymes in Mandel's medium. In 10 L jar fermenter, maximum amylase and FPase activities, 3.25 and 0.23 U/mL, were obtained when the culture was grown at 30$^{\circ}C$, 200 rpm and 0.6 vvm for 3 days. In 100 mL flask level and 10 L jar fermenter, amylase produced by the strain 15 showed similar cellulolytic and amylolytic enzyme activities with Trichoderma inhamatum KSJ1 isolated from rotten woods by previous researcher. The ability of saccharification to food wastes also showed similar degree. However, the isolate 15 appeared to be yellowish in YMEA plate comparing to Trichoderma inhamatum KSJ1 in greenish.

Decentralized Composting of Garbage in a Small Composter for Dwelling House II. Changes in Microbial Flora in laboratory Composting of Household Garbage in a small Bin (가정용 소형 퇴비화 용기에 의한 부엌쓰레기의 분산식 퇴비화 II. 실험실 조건에 있어서 미생물상의 변동)

  • Lee, Yon;Joo, Woo-Hong;Seo, Jeoung-Yoon
    • Korean Journal of Environmental Agriculture
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    • v.13 no.3
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    • pp.338-345
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    • 1994
  • In the course of developing a small composter for dwelling house, we designed two different small bins; one is insullated (type 1) and the other uninsullated (type 2). Several interesting results were abtained from the study using these bins for garbage composting in winter, spring and summer. Changes in microbial number were very similar to those observed in the general composting process. However, microbial flora was relatively simple. The genera Streptomyces and Nocardia of actinomycetes and the genera Aspergillus, Penicillium, Mucor, Absidia, Rhizopus of hypomycetes was observed from the composted materials. Thermophiles secreted most of the ${\alpha}-amylase$ secreted in winter but mesophilic actinomycetes did in summer. The amount of secreted protease was much lower in winter than in summer. Lipases were secreted more by mesophiles than thermophiles. Only Aspergillus of hypomycetes was observed to degrade cellulose. Generally, the appearance of enzyme producing microorganisms increased in summer than in the other seasons. In the point of seasonal increase of temperature and changes in microbial flora, the number of microorganisms was higher in summer or spring than in winter.

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