• Journal of Broadcast Engineering
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• v.27 no.6
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• pp.923-935
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• 2022

This paper deals with detection and classification of leaf diseases for phenomics systems. As the smart farm systems of plants are increased, It is important to determine quickly the abnormal growth of plants without supervisors. This paper considers the color distribution and shape information of leaf diseases, and designs two deep leaning networks in training the leaf diseases. In the first step, color distribution of input image is analyzed for possible diseases. In the second step, the image is first partitioned into small segments using mean shift clustering, and the color information of each segment is inspected by the proposed Color Network. When a segment is determined as disease, the shape parameters of the segment are extracted and inspected by proposed Shape Network to classify the leaf disease types in the third step. According to the experiments with two types of diseases (frogeye/rust and tipburn) for apple leaves and iceberg, the leaf diseases are detected with 92.3% recall for a segment and with 99.3% recall for an input image where there are usually more than two disease segments. The proposed method is useful for detecting leaf diseases quickly in the smart farm environment, and is extendible to various types of new plants and leaf diseases without additional learning.

• Korean Journal of Environmental Agriculture
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• v.40 no.4
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• pp.366-372
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• 2021

BACKGROUND: Ammonia is known as a precursor to fine particulate matter, and according to CAPSS, annual ammonia emissions in the agricultural sector were 249,777 tons as of 2018, accounting for about 79.0% of Korea's total ammonia emissions. In particular, ammonia emissions from agricultural land increased by 19,566 tons (10.2%) compared to the previous year. The Ministry of Environment is setting emission statistics using the ammonia emission coefficient developed in Korea in 2008, but researchers in the agricultural field regard it as a coefficient that does not reflect the reality of Korea's agricultural environment. Accordingly, in order to develop ammonia emission coefficients from the cultivation of apples and pears, Korea's representative fruit type, test agricultural land was set in Iksan, Jeollabuk-do. METHODS AND RESULTS: This study attempted to obtain the ammonia emission coefficient by the treatment of the composite fertilizer (N-P₂O₅-K₂O=12-7-9), and the flux was measured using a dynamic flow-through chamber method. As for the chamber, a total of 12 chambers were installed repeatedly in 4 zones and used to develop emission coefficients. Using compound fertilizers during fruit tree cultivation, the ammonia emission coefficient was evaluated as 10.4 kg NH3/ton for pears and 15.3 kg NH₃/ton for apples. The reason why the ammonia emission coefficient according to the use of composite fertilizers was calculated higher for apple cultivation is believed to be due to the relatively high pH concentration of apple orchard soil. CONCLUSION(S): This study may provide basic data for upgrading the ammonia emission coefficient when using composite fertilizers in agricultural land. In the future, it might be necessary to upgrade the calculation of emissions through the development of ammonia and fine particulate matter emission coefficients considering the agricultural environment of Korea.

• Korean Journal of Plant Taxonomy
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• v.52 no.3
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• pp.184-195
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• 2022

The invasive alien species Brassica tournefortii Gouan (Brassicaceae) is herein reported for the first time in Korea, from Gunsan-si, Gochang-gun, and Jeju-si. Brassica tournefortii can easily be distinguished from B. juncea and B. napus by its dense stiff hairs at the base of the stem and leaves, basally and distally branched stems, partially dehiscent fruits, and seeds that become mucilaginous in the presence of moisture. Although some taxonomists have classified this species as belonging to Coincya Rouy based on its fruit and seed characteristics, the existence of one vein on the fruit valves and our maximum likelihood analysis using internal transcribed spacer sequences placed it in Brassica. Distribution data, photographs, and a description of B. tournefortii are presented herein. Moreover, potential changes in the distribution of B. tournefortii were predicted under different climate scenarios, but our analysis showed that the probability of the spreading of this species is low. Nevertheless, continuous monitoring is necessary for an accurate assessment. The results of the present study can be used to conduct an invasion risk assessment and can assist with the effective management of this invasive alien species.

• The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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• v.35 no.4
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• pp.63-74
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• 2022

Objectives : Skin aging is generally characterized by wrinkles, sagging, loss of elasticity roughness, pigmentation and dryness. This changes is caused by reducing the elements constituting the extracellular matrix contributing to the physiological properties of the skin, such as collagen fiber, elastic fiber, and hyaluronic acid. Adequate skin hydration is important to maintain normal skin function and reduce skin aging. The present study is objective to observe skin moisturizing effects of Unripe apple(UA, Immature fruit of Malus pumila Mill) in vivo and its underlying molecular mechanisms. Methods : ICR mice were orally administerd UA(100, 200 and 400mg/kg/day) for 8 weeks, and skin water contents and the expression of transforming growth factor (TGF)-𝛽1, ceramide, hyaluronan and collagen type I(COL1) were measured in dorsal back skin of the mice. Gene expression of hyaluronan synthase(HAS1, HAS2, HAS3), collagen synthase(COL1A1, COL1A2) and TGF-𝛽1 were also determined by realtime RT-PCR. Results : Skin water contents and the expression of TGF-𝛽1, ceramide, COL1 and hyaluronan were significantly increased in UA group(100, 200 and 400mg/kg/day) compared to vehicle control. The mRNA expression of HAS isoform(HAS1, HAS2, HAS3), COL1A1, COL1A2, and TGF-𝛽1 were also significantly increased by UA. Conclusions : UA has skin moisturizing effects and enhancement activities in skin function related components(COL1, hyaluronan, ceramide and TGF-𝛽1). These results suggested that UA can be a developing candidate for developing alternative skin protective agent or functional food ingredient.

• The Journal of the Convergence on Culture Technology
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• v.8 no.6
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• pp.943-951
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• 2022

In this study, 8 kinds of fruits and vegetables such as apples, pears and radishes were cut and hot water extracts and Steamed hot water extract from fruits and vegetables were prepared and used as experimental substrates. As a result of fermenting with 1% (W/V) red ginseng extract (W/V) and 8 types of lactic acid bacteria mixed starter added to the lactic acid bacteria fermented extract, the pattern and content of ginsenosides were almost unchanged in the fruit and vegetable extract group and the steam treatment group. However, in the lactic acid bacteria fermented group, the TLC pattern was changed according to the fermentation process and treatment, and the content of ginsenosides converted into Rg₃(S) and Rg₅ increased. No change in the number of lactic acid bacteria (cfu) was observed in all four types of fruit and vegetable extracts. The number of lactic acid bacteria CFU was slightly decreased in the four fermented groups of fruit and vegetable extracts, but the growth inhibitory effect of beneficial bacteria was not significant. The growth inhibitory effect of the three harmful bacteria was not affected by the growth of E. coli and Pseudomonas in the four fruit and vegetable extracts. However, the proliferation of Salmonella was inhibited, which was confirmed as the growth inhibitory effect of the fruit and vegetable extract regardless of whether the steamed hot water extract or red ginseng extract was added.

• Journal of Food Hygiene and Safety
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• v.37 no.4
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• pp.238-248
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• 2022

This study was conducted to evaluate the safety of 208 types of residue pesticides on 150 dried agricultural products in Ulsan. The pesticide residues were detected using GC-MS/MS, LC-MS/MS, GC/ECD, GC/NPD, and LC/PDA. The detection rate was 19.3% (29 of 150 samples), and 28 types of pesticides were detected. Two of the 29 pesticide residues exceeded the maximum residue limit (MRL). Difenoconazole was detected in Ulleungdo aster, and chlorothalonil and chlorpyrifos were detected in chili pepper leaves. In the vegetable group, the frequency of pesticide residues was found to be the highest in dried leafy vegetables, followed by dried fruiting vegetables other than cucurbit vegetables, and then dried stalk and stem vegetables. The pesticide types detected in the commercial dried agricultural products were fungicide (60%), insecticide (23.8%), and acaricide (16.3%). In the validation study, the values of limit of detection (LOD), limit of quantitation (LOQ), coefficient of determination (R²), and recovery rate were in the range of 0.0001-0.0409 mg/kg, 0.0003-0.1241 mg/kg, 0.994-0.999, and 81.58-116.79%, respectively. The ratio of estimated daily intake (EDI)/acceptable daily intake (ADI) was 0.00002-0.31395%.

• Journal of Applied Biological Chemistry
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• v.65 no.4
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• pp.405-412
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• 2022

Echinodorus cordifolius (L.) is an aquatic plant in the family Alismataceae. The anti-skin aging activity of E. cordifolius (L.) has not been yet reported. Therefore, the objective of the present study was to prepare 70% ethanol extract (ECEE) from E. cordifolius (L.) and investigate their antioxidant and anti-hyaluronidase activities for confirm the potential of anti-skin aging. ECEE showed good activities of DPPH, hydrogen peroxide scavenging, and hyaluronidase inhibition, with EC₅₀ and IC₅₀ values of 31.4, 300, and 450 ㎍/mL, respectively. ECEE also significantly improved cell viability and inhibited intracellular reactive oxygen species dose-dependently against 1 mM hydrogen peroxide-induced oxidative stress in immortalized human keratinocytes (HaCaT cells). Furthermore, ECEE upregulated hyaluronic acid (HA)-synthesizing enzyme hyaluronan synthase 2 (HAS2) expression level, but downregulated expression level of HA-degrading enzyme hyaluronidase 2, resulting in increased HA production in HaCaT cells. Taken together, these results suggest that ECEE shows antioxidant and anti-hyaluronidase potential and could be a functional cosmetic ingredients for anti-skin aging.

• Research in Plant Disease
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• v.29 no.1
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• pp.79-81
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• 2023

During the nationwide survey of fire blight, the typical shoot blight symptoms were found on Korean mountain ash (Sorbus alnifolia) which was located near an orchard that produced fire blight on pear trees in Eumseong, Korea, May 2021. To identify the causal agent, we progressed isolation from the symptomatic leaves and shoots. Two white and mucoid colonies were isolated into the pure culture. Two isolates were identified as Erwinia amylovora according to the colony-polymerase chain reaction (PCR) with amsB primers and the phylogenetic tree using 16S rRNA sequences. To test of pathogenicity of two isolates, we inoculated immature pear fruits and understock of apple. We observed necrosis and oozes on immature pear fruits and shoot blight resulting in necrosis on apple shoots six days after inoculation. Colonies were recovered from the inoculated pears and apples, and identity was confirmed through colony PCR for amsB genes. To our knowledge, E. amylovora was first reported on Korean mountain ash native to South Korea.

• Journal of the Daesoon Academy of Sciences
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• v.21
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• pp.29-97
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• 2013

뇌법은 도교의 중요한 법술로서 당송(唐宋)이래 매우 흥했는데, 북송의 임영소 등이 신소파(神霄派)를 개창한 이후에 도교 법문을 대표하는 영역이 되었다. 그로부터 각종의 뇌법이 천하에 성행하였고, 도교에는 새로운 뇌신들이 거듭 나타나게 되었다. 그 대표적인 것이 뇌법신앙의 구진천존(九宸天尊)인데, 이 아홉 천존들은 도교의 최고신인 삼청성조(三清聖祖)의 통치 하에 있는 것으로 간주되었다. 『옥추보경』은 북송 때 탄생하였는데 이 경전이 세상에 널리 발간되어 구천응원뇌성보화천존에 대한 신앙이 형성된 것은 남송 시기 백옥섬(白玉蟾) 이후이다. 『옥추보경』은 구천응원뇌성보화천존의 법문(法門)을 선양하기 위한 방책으로 천존의 성호를 부를 것을 권장한다. 법문을 염송하는 것은 매우 쉽고도 간단하여 누구나 할 수 있었으므로 사회적으로 크게 확대되었다. 특히 『봉신연의』와 『서유기』와 같은 문예소설에 뇌성보화천존이 등장함으로 해서, 민중들은 뇌성보화천존을 더욱 널리 숭배하게 되었다. 현재도 중국의 민간에서 이 신격에 대한 신앙은 매우 보편적이다. 구천응원뇌성보화천존은 옥청진왕(玉清真王)의 화신(化身)이다. 뇌성보화천존은 시방에 그 형체를 나타내고, 중생들을 고뇌로부터 구제하고 이끈다. 36천을 주재할 권능이 있으며, 자비로운 모습으로 모든 겁에 응한다. 또 뇌성보화천존의 주변에는 뇌공(雷公), 우사(雨師), 풍백(風伯)을 비롯한 십대원사(十大元帥)와 등백온원사(鄧伯溫元帥), 신한신원사(辛漢臣元帥) 등의 신장들이 보인다. 이러한 신장들은 양송(兩宋) 시대에 출현하였으며 대다수가 뇌부의 제신들이다. 뇌성보화천존 신앙을 구현하기 위한 재초 의식에는 천지 제신(諸神)이 강림하기를 청하는 뇌정사과(雷霆謝過)와 죄를 뉘우치고 복을 비는 참의(懺儀) 또는 참회문식(懺悔文式) 등이 있다. 이런 법식들은 매우 전문적인 것이고, 일반인들에게는 법문을 염송하는 간편한 방법인 십자천경법(十字天經法)이 널리 퍼졌다. 즉 늘 경건한 마음으로 천존의 성호를 오랫동안 염송하게 되면 공덕원만(功德圓滿)의 경지에 도달할 수 있다는 것이다. 『옥추보경』은 민중의 세계에 깊은 영향을 끼치고 광범위하게 알려져 그 주해나 주석본만도 이십여 종에 이르렀고 송원 이래의 핵심적인 도교 문헌의 하나로 자리매김하였다. 『옥추보경』은 일본과 한국 등지에도 급속히 전파되었다. 한국에는 고려시대에 신소뇌법(神霄雷法)이 전해졌는데, 특히 고려 예종 13년(1118년) 7월에 중국 도교 의학이 고려에 전승되었을 때의 도교 의학이 『옥추보경』을 위주로 한 치료법이었다. 그리고 그 흔적은 조선의 허준이 주편한 『동의보감』에서도 찾아볼 수 있다. 조선의 민간에서는 많은 사람들이 『옥추보경』을 송독하였고 거기에 수록된 부록(符籙)을 운용하였으며 이로써 병을 몰아내고 잡귀를 물리쳐 생명을 지키기를 바랐다. 현대 한국에서는 구천응원뇌성보화천존강성상제에 대한 신앙이 크게 성행하고 있으니, 그 종단이 바로 대순진리회이다. 대순진리회는 증산 성사께서 인세에 강림하시기 전에 구천대원조화주신이며, 화천 후 구천응원뇌성보화천존상제의 위에 임하였다고 신앙한다. 즉 증산이 천존상제의 화신이라 믿는 것이다. 이것과는 좀 다르지만, 인간이 신의 화신이 되는 신성한 변화를 뇌법에서는 '변신(變神)'이라고 부른다. 변신은 내련(內煉)을 행할 때나 시법(施法)시에 도인들이 필히 진입해야 할 특이한 정신 상태로서, 자아의 존재를 망각하고 신진(神真)으로 변화하여 인신합일(人神合一)을 이루는 것을 의미한다. 이러한 변신은 대순진리회의 수도 목적인 도통과도 한편으로는 유사한 면이 있다고 생각된다. 대순진리회가 목적하는 지상신선실현이 의미하는 것은, 사람이 도를 깨닫는 노력을 통해 신의 경지에 도달하는 것이 아니라 인간의 본질과 천성을 회복하여 이에 상응하는 신과 합일하는 것이라는 점에서 뇌법의 변신과 유사한 점이 있다고 할 것이며 이에 대해서는 추후 연구과제로 남겨둔다.

• Korean Journal of Agricultural and Forest Meteorology
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• v.25 no.4
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• pp.302-310
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• 2023

In this study, the extent of the impact of rainfall on the outbreak of fire blight during the blossom infection risk period was explored. In the Chungnam province, the outbreak of fire blight disease began in 2015, and changes in the outbreak's scale were most pronounced between 2020 and 2022, significantly escalating from 63 orchards in 2020 to 170 orchards in 2021, before decreasing to 46 orchards in 2022. In 2022, the number of incidence has decreased and the number of canker symptom in branches has also decreased. It was evaluated that the significant decrease of fire blight disease in 2022 was due to the dry weather during the flowering season. In other words, this yearly fluctuation in fire blight outbreaks was correlated with the presence or absence of rainfall and accumulated precipitation during the blossom infection risk period. This trend was observed across all surveyed regions where apples and pears were cultivated. Among the weather conditions influencing the blossom infection risk period, rainfall notably affected the activation of pathogens from over-wintering cankers and flower infections. In particular, precipitation during the initial 3 days of the blossom infection risk warning was confirmed as a decisive factor in determining the outbreak's scale.