• Korean Journal of Plant Tissue Culture
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• v.24 no.1
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• pp.43-48
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• 1997

Apical meristems of Wasabia japonica were cultured on Murashige and Skoog's medium supplemented with cytokinins alone or together with 1.0 mg/L IAA. Shoot initials could be induced from leaf primordia on apical meristems. Calli and roots were formed on the medium containing cytokinins and 1.0 mg/L IAA in combination after 30 days of culture, but there were no callus proliferation. Shoot organogenesis began after 60 days of culture and these small shoots elongated when transferred to a medium containing 1.0 mg/L BA or kinetin. Shoots were formed directly without callus induction from apical meristems all the explants on the medium containing cytokinins variously, and most of the shoots proliferated multiple shoots which could be divided to obtain plantlets. Shoot multiplication rate in response to cytokinins was best on the medium containing 1.0 mg/L BA or 2.0 mg/L zeatin. Divided plantlets rooted well on MS medium containing 0.01 mg/L IBA after 15~30 days of subculture and the rooted plantlets developed into whole plants with multiple shoots. After rooting, the regenerated plants were washed and transferred to the pots containing sterilized soil.

• Journal of Life Science
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• v.15 no.3 s.70
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• pp.499-504
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• 2005

This experiment was conducted to identify the effect of several plant growth retardants on growth of Sedirea japonica seedlings cultured in vitro and their changes of invertase activities. When seedlings of Sedirea japonica were treated with ancymidol and paclobutrazol, as the concentrations were increased, leaf length was gradually shortened and leaf width became wider than that of control. On the other hand, root length was shorter, but the number of root and the root's diameters were greatly increased, compared with control. In 0.05mg/L uniconazole, growth of leaf and root were enhanced, compared with the control and higher concentrations of uniconazole. As concentration of each growth retardants was increased, leaf shape became round and smaller. Both soluble acid invertase activity and soluble alkaline invertase activity in leaf were decreased in higher concentrations of each growth retardant, but those of the root were contrary to those of the leaf. To confirm the estimated invertase activities, starch content of leaf was higher in low concentration treatments in each growth retardant, but in the root was contrary to content that of the leaf.

• Proceedings of the Korean Environmental Sciences Society Conference
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• 2008.11a
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• pp.165-167
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• 2008

토양 속에 첨가된 납의 농도가 증가하면 애기장대 식물체 내에 축적된 납의 농도도 증가하였는데, 특히 오염물질 배출기준농도보다 50배 높은 납이 첨가된 토양에서 생장한 식물체내에 축적된 납 농도는 정상식물보다 약 2.6배 증가하였다. 이러한 결과는 토양 속에 오염된 납은 식물의 줄기나 일보다는 뿌리에 더 많이 축적되며, 줄기와 잎에 축적되는 납농도는 토양 속에 오염된 납 농도에 비례하여 증가하지 않으나 뿌리에서는 농도에 비례하여 매우 증가하였음을 나타내고 있다.

• Korean Journal of Medicinal Crop Science
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• v.7 no.4
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• pp.275-281
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• 1999

Embryogenic calli of Codonopsis lanceolata were cultured on MS agar medium containing various concentrations of sucrose as a carbon source. Upon transfer to MS basal medium, somatic embryos of cotyledonary stage converted to plantlets. When sucrose was added with greater than 4%, the number of shoots and roots regenerated from somatic embryo increased. However, the growth of shoots and roots was retarded in agar medium with more than 2% sucrose, but promoted in medium with lower concentration of sucrose. Saponin contents of shoots regenerated from somatic embryos, embryogenic calli, non-embryogenic calli, and native roots were determined by HPLC. Saponin contents of native root was variable, depending on regenerant, embryogenic calli, and cotyledonary embryos. The saponin contents of regenerated roots in medium with high sucrose was similar to native roots. Saponins content based on cell differentiation to shoot and root was dramatically decreased. This results could be effectively controlled for the production of useful secondary metabolites.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2018.04a
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• pp.82-82
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• 2018

본 연구는 국내에 자생하는 자원식물들의 살초활성을 조사하여 식물생장억제물질을 활용한 환경친화형 제초제 개발에 요구되는 기초 자료를 확보하기 위해 수행되었다. 국내 자생 식물 101종을 식물체 부위별로 나누어 MeOH로 추출하여 획득된 시료 129점에 대해 돌피(Echinocholoa crus-galli P.B. var. formosensis Ohwi)를 이용한 살초활성 검정을 실시하였다. 시료를 소량의 methanol로 녹여 직경 5.5cm petri dish 상의 Whatman No. 2 여지에 균일하게 흡착시킨 후, fume hood 내에서 용매를 제거하고 1mL의 증류수를 첨가하였다. 돌피 종자를 15립씩 3반복으로 파종하여 $28^{\circ}C$, 5,000lux의 growth chamber에 치상하고, 7일 후 시험구 당 균일하게 자란 10개의 유묘를 선발해 초장 및 근장을 조사하였으며, 살초활성 평가를 위해 대조구 개체의 초장과 근장에 대한 생장억제율을 산출하였다. $1,000{\mu}g/mL$ 농도에서 101종 129점 MeOH 추출물들의 돌피 유묘 뿌리 생장에 대한 억제율을 조사한 결과, 80% 이상을 나타낸 식물 시료는 가죽나무 줄기, 누린내풀 지상부, 단풍취 뿌리, 두릅나무 지상부, 백양꽃 전초, 백양꽃 지하부, 병조희풀 경엽부, 산사나무 잎 가지, 삽주 뿌리, 상사화 전초, 상사화 지하부, 석산 뿌리, 애기송이풀 전초 등 17점이었다. 이들 중 90% 이상 고활성을 나타낸 단풍취 뿌리, 두릅나무 지상부, 백양꽃 전초, 상사화 지하부, 석산 뿌리 유래의 추출물에는 새로운 환경친화형 제초제 개발소재로써 활용할 수 있는 살초활성물질들이 함유되어 있는 것으로 생각된다.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2020.08a
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• pp.28-28
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• 2020

본 연구는 식용, 목재용, 가구용 등 유용한 상수리나무의 효율적인 양묘를 위한 기초 정보를 제공하기 위하여 용기의 종류에 따른 유묘 생장을 측정하고 조사하였다. 용기별 유묘의 엽수는 15구가 18.6개로서 24구의 18.5개보다 많았고, 엽건물중은 15구가 2.41g으로서 24구의 2.24g보다 가벼웠다. 줄기길이는 15구가 33.5cm로서 24구의 34.6cm보다 짧았고, 줄기직경은 15구가 4.48mm로서 24구의 4.77mm보다 좁았으며, 줄기건물중은 15구가 1.40g으로서 24구의 1.53g보다 가벼웠다. 뿌리길이는 15구가 39.2cm로서 24구의 49.1cm보다 짧았고, 뿌리건물중은 15구가 7.60g으로서 24구의 6.92g보다 무거웠다. 그리고 식물체건물중은 15구가 11.4g으로서 24구의 10.7g보다 무거웠다. 지상부와 지하부 (S/R) 비율은 15구가 0.50으로서 24구의 0.55보다 낮았다. 이와 같이 재배 용기의 크기에 따른 상수리나무 유묘의 생장은 여러 생장 측정 중 뿌리길이를 제외하면 T-검정에서 차이를 나타내지 않았다. 이렇게 보면 상수리나무는 용기 크기와 유묘 생장의 상관관계가 없다고 할 수 있으나 본 실험에서 노천매장 종자의 파종상 유묘를 선별하여 이식할 때 물리적 손상이 있을 수 있어 추후 추가적인 보완 실험이 필요하였다.

• Korean Journal of Environment and Ecology
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• v.7 no.1
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• pp.6-9
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• 1993

To examine aluminum toxicity on woody plants, Forsythia koreans and Platanus occidentalis cuttings were grown in the pot(48$\times$33$\times$9cm) filled with sand, and treated aluminum solution and ground water (pH 6.75) 3times per week from April 28, 1993 to June 16. Aluminum solution were prepared 1.0, 2.5 and 5.0mM aluminum potassium sulfate, dilulted with ground water. Growth-related characters (Shoot growth, leaf number and leaf chlorophyll content ) and root growth were measured and compared among the treatments. In all growth-related characters(Shoot growth, leaf number and leaf chlorophyll content), differences among the treatments were highly significant. In root growth, differences among the treatments were highly significant for Forsythia koreana cuttings, but not for Platanus occidentalis cuttings.

• Agrochemical news magazine
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• v.14 no.3 s.114
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• pp.68-71
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• 1993

• 수목보호
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• s.2
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• pp.11-13
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• 1996

• Asian Journal of Turfgrass Science
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• v.20 no.2
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• pp.167-174
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• 2006

This study was initiated to investigate the effect of chitosan on creeping bentgrass growth. Chitosan was applied several times in dilution of 300, 500 and 800 times at ten-day intervals after transplanting. Such growth characteristics as leaf length, root length, numbers of leaves, fresh weight and dry weight and chlorophyll content were observed. Treatment of 500 times diluted chitosan resulted in the longest root length, being 31.5cm while the control the shortest root of 25.1cm. Leaf numbers were 27.9 and 45.5, respectively for the control and the 300 times treatment. The highest chlorophyll content was associated with treatment of 300 times diluted chitosan and the lowest one with the control, resulting in 11.9 and $18.4mg/100cm^2$, respectively. We found that leaf number, chlorophyll content, fresh and dry weight were higher in the treatment of 500 times than the other treatments.