• Applied Biological Chemistry
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• v.47 no.2
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• pp.192-196
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• 2004

In order to measure cellular physiological activity including ion channel activity, protoplasts were isolated from the root tissue of tomato plant. The general methods recommended were not efficient enough to make protoplasts from the root tissue. Among various conditions tested, we found that a two-step treatment of osmosis is very efficient for the isolation of protoplasts. In this procedure, root tissues were preincubated in a solution containing 300 mM sorbitol for 30 min. Then, they moved to the reaction solution containing 700 mM sorbitol as well as cell wall-digesting enzymes. The formation of protoplast was greatly increased by this method. In order to find the optimal condition of the two-step method, various conditions of pH, osmotic pressure, incubation time, and the concentrations of cell wall-digesting enzymes were tested. The yield of protoplast isolation was maximal at pH 5.0 after 2 hr incubation. Mixed enzymes of 3% cellulase, 1 % macerozyme, and 0.1 % pectolyase showed maximal protoplast isolation. The physiological activity of isolated protoplast evaluated by measuring the cellular ATPase activity was as high as that measured from the preparation of root tissue. The protoplasts isolated by this method were remained healthy up to 4 hrs which is enough time to measure the cellular physiological activity. These results show that the two-step treatment of osmotic pressure was successful to obtain high yield of healthy protoplast from tomato root tissue.

• Journal of Bio-Environment Control
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• v.15 no.2
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• pp.162-166
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• 2006

The objectives of this study were to determine the effect of cultivation time on the incidence of brown fruit stem (BFS) in glasshouse sweet pepper (cv. Special) and to investigate environmental causes of this disorder. The plants transplanted on 31 August (T1) showed more incidence of BFS than those on 24 November (T2) (6.1% vs.2.9%; P<0.01). The BFS symptom began to appear after completion of fruit enlargement, more often around fruit coloring period. Comparing the environmental factors between T1 and T2, with their data collected for 3 weeks around fruit coloring period, the factor that was most likely responsible for BFS incidence was found to be the night-time humidity deficit (HD) ($1.9g{\cdot}m^{-3}\;vs\;2.9g{\cdot}m^{-3}HD$). These results were reconfirmed as T1 was compared to the plants (T3) that were transplanted at a similar time of the following year to T1, but designed to reduce BSF by increasing air HD via heating at night. That is,T3 had much higher night-time HD than T1 ($5.9g{\cdot}m^{-3}\;vs\;1.9g{\cdot}m^{-3}HD$), and showed no incidence of BFS. These results indicated that, to prevent BFS incidence in the winter-harvesting sweet pepper plants, air humidity at night should be controlled low, especially for the fruit coloring period after fruit enlargement period is completed.

• Proceedings of the Korea Water Resources Association Conference
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• 2005.05b
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• pp.1383-1387
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• 2005

사면의 식생이 초기에는 강우에 의해서 표면흐름, 지하수위 증가, 지표수의 침윤에 의해서 성장이 될 수 있으나, 시간이 경과함에 따라 식생에 의한 사면의 안정성을 확보 하는 데에는 큰 영향을 미치지 못한다. 즉, 사면의 식생을 복우언시켜 식물근(뿌리)에 의해서 사면을 안정화 시키기 위해서는 사면의 위치, 고도 및 방향 등에 따라 적응할 수 있는 식물의 선택과 성장에 충분한 수분을 보습할 수 있는 구조의 사면계획과 구조물 설치가 필수적이다. 본 연구에서는 절$\cdot$성토면에서 경사면의 붕괴를 방지하고 식생을 복원하는 방법으로 Mattress Gabion을 제안하였다. 정형화되고 유연성 있는 다공성 Mattress 구조를 통한 식생의 복원은 사면의 안정과 환경친화적인 목적을 모두 만족할 수 있었다. 특히, Gabion 근고공이 설치된 이후에 공극이 큰 Mattress 구조물을 중심으로 식물의 뿌리를 지반에 직접 연결하거나, 식생 활착이 어려운 암반 절취 사면에서도 Mattress 옹벽을 통해서 식물이 양질의 흙에 직접 활착할 수 있도록 설계한 사면 Mattress의 경우는 사면의 안정성 증대와 아울러 지하수의 표면흐름과 투수 및 침윤에 의한 식물의 성장피해를 최소화하고 시간이 지날수록 식생에 의해서 Mattress Gabion 옹벽이 내구성과 유연성을 유지할 수 있었다.

• Proceedings of the Korean Vacuum Society Conference
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• 2010.08a
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• pp.47-47
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• 2010

게이트 밸브는 진공 시스템 구성에 있어서 빠질 수 없는 중요한 부품 중 하나로 공간을 구획하여 필요에 따라 자유롭게 진공 상태와 대기 개방 상태가 공존하도록 만들어 주며 진공펌프와 같은 주요 기기들의 탈착 및 교체를 용이하게 해 준다. 게이트 밸브는 열린 상태에서는 저항이 최소화되는 반면 닫힌 상태에서는 마치 마구리 플랜지로 막은 것처럼 완벽한 차단이 요구된다. 일반적으로 게이트 밸브 상용제품은 개스킷이 닿는 밸브 시트가 한쪽에만 마련되어 있으므로 디스크 기계구조가 충분한 압력을 발휘하지 못한다면 어느 쪽이 대기압인가에 따라 디스크를 미는 힘의 크기가 변하여 기밀특성이 달라질 수 있다. 따라서 게이트 밸브의 누설검사는 순방향과 역방향 모두에 대해 시행해야 한다. 양방향 누설검사를 위해 한쪽 밸브 공간을 배기하고 반대쪽 공간에 헬륨을 뿌리는 작업을 교대로 하려면 배기시간의 단축과 신속한 잔류 헬륨기체 제거가 필수적이다. 게이트 밸브 제조 공장에서 상품을 출하하기 위한 시험검사라는 것을 전제로 합리적인 시간 내에 정확한 누설검사를 일관성 있게 할 수 있는 적절한 누설검사 시스템을 설계하고 측정절차를 상용 게이트 밸브에 대해 적용해 보았다.

• Journal of Bio-Environment Control
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• v.16 no.3
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• pp.174-179
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• 2007

Although the relationship between fermentation and factors such as soil water, redox potential, rootstocks and climatic conditions has been reported, its mechanism of fermentation is still not clear. Transpirations of leaf and fruit at different climatic conditions, influence of soil water potential and atmospheric vapor pressure deficit (VPD) on fermentation were evaluated. Transpiration rate decreased with decreasing soil temperature and soil water potential. Low VPD conditions which occurred during low air temperature and high humidity also decreased transipration rate. These data exhibit that fruit water balance affected by various factors relate to transpiration. Our results also indicate that high hydraulic conductance of root, high soil water potential and low VPD condition exert a significant effect on fermention of oriental melon and so called "water filled fruit".

• Asian Journal of Turfgrass Science
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• v.25 no.2
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• pp.125-132
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• 2011

Drought is a major limiting factor in turfgrass management. Turfgrass responses to water deficit depend on the amount and the rate of water loss as well as the duration of the stress condition. This review paper was designed to understand responses such as photosynthesis, canopy spectral reflectance, plant cell, root, hormone and protein alteration when turfgrass got drought stress. Furthermore, mechanisms to recover from drought conditions were reviewed in detail. However, there are still many questions regarding plant adaptation to water deficit. It is not clear that the mechanism by which plants detect water deficit and transfer that signal into adaptive responses. Turfgrass research should focus on the best management practices such as how to enhance the ability of self-defense mechanism through understanding plant responses by environmental stress.

• Proceedings of the Korean Society for Agricultural Machinery Conference
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• 2002.02a
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• pp.323-328
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• 2002

양파의 껍질에 칼금을 내고 꼭지와 뿌리를 절단한 후 회전하는 두 개의 롤러 위에 양파를 낙하시켜 구르게 하면서 그 위에 고압공기를 분사시켜 양파의 외각 껍질을 제거하는 공기분사식 박피장치의 최적 작동조건을 구명하기 위하여 수행한 시험결과를 요약하면 다음과 같다. 1, 박피장치에 고압공기를 분사하지 않았을 때, 롤러의 원주속도가 빠를수록, 양파의 무게가 가벼울수록 구름소요시간은 줄어들었고 직경방향 구름율이 가장 높았는데, 직경방향 구름소요시간이 가장 짧게 나타나는 주롤러의 원주속도는 2.4 m/s이었다. 2. 주롤러의 원주속도 2.4 m/s일 때 양파의 무게, 분사공기압 및 칼금수가 증가할수록 박피율은 증가하였으며, 박피율이 가장 높은 조건은 칼금수 4개, 분사공기압 3.5 kg/$cm^2$이상이었다. 3. 박피소요시간은 칼금수가 많을수록, 분사공기압이 높을수록 짧아지는 것으로 나타났고, 칼금수를 4개로 하고 분사공기압을 4.0 kg/$cm^2$이상으로 하면 박피소요시간을 2초 이하로 유지할 수 었다. 4. 양파의 껍질강도는 저장기일과 조건에 따라 다르므로 모든 양파의 박피를 위해서는 분사 공기압을 4.0 kg/$cm^2$이상으로 하고 공기분사시간을 2초에서 5초가지 조절할 수 있는 장치를 부착하는 것이 좋을 것으로 판단된다. 5. 시험에 사용된 양파는 무게는 150~350$\pm$30 g, 직경 60~120 mm의 크기로 우리나라에서 생산되는 양파의 98%에 적용이 가능할 것으로 생각된다.

• Journal of Bio-Environment Control
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• v.18 no.4
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• pp.303-308
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• 2009

This study was conducted to analyze the feasibility of plant growth under low pressure and to investigate the effect of pressure on plant growth. Three levels of pressures (25, 50, and 101.3 kPa (control)) were provided to analyze the growth of Lettuce (Lactuca sativa L.) as affected by low pressure. Photoperiod, air temperature, and photosynthetic photon flux were set at 16/8 h, 26/$18^{\circ}C$, and $240{\mu}mol{\cdot}m^{-2}s^{-1}$, respectively. Growth characteristics of lettuce were measured on 7 days and 14 days after experiment. Leaf length, leaf width, leaf area, and root dry weight of lettuce measured on 7 days under 25 and 50 kPa were significant as compared to the control. Leaf length, top dry matter and root dry matter of lettuce measured on 14 days were significantly different under 25 and 50 kPa. From these results, we confirmed that lettuce could be grown under low pressure. However high relative humidity by evapotranspiration from leaves and growing beds under low pressure caused the condensation on the inner surface of the chamber. Therefore in a low pressure chamber, humidity control is required to maintain the relative humidity at a proper level.

• Journal of Bio-Environment Control
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• v.16 no.4
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• pp.333-337
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• 2007

This study was conducted to assess effects of soil moisture condition and ventilation on development of fermented fruit of oriental melon. In higher soil moisture condition (-10 kPa), roots absorbed more water and transpiration was decreased under low temperature and high humidity conditions. Development of fermented fruit may be come from absorbed water concentrated placenta with higher soluble solid. Fermented fruit was developed with higher level at Shintozoa and Elite, was developed with lower level at Hongtozoa, and was not developed at self-rooting of oriental melon. Ventilation on fruit did not affect development of fermented fruit at Shintozoa, Elite, Hongtozoa and self-rooting seedling. Ca contents of flesh and placenta of fruit under ventilation treatment were higher than control but contents of Acetaldehyde, Ethanol and Ethylacetate were lower.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.40 no.4
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• pp.481-486
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• 1995

The salt stress at seedling stage of winter barley was examined in different concentrations of NaCl containing 1/2 Hoagland solution. Fresh weight of seedling at 30 days after seeding was highest at 25mM of NaCl concentration containing 1/2 Hoagland solution but if the NaCl concentration was more than 50mM it began to decrease seriously. Water content in plant was decreased according to increase of NaCl concentration in 1/2 Hoagland solution, so physiological mechanism of NaCl in barley was different from saline plant. Stoma number per cm$^2$ of first leaf was higher than that of control in case of stressed by NaCl but in that case the leaf length was decreased so the number of stoma per first leaf was slightly decreased. Chloroplast shape was not changed by 75mM of high NaCl contained 1/2 Hoagland solution but cell division at root growing point was inhibited by 75mM of NaCl. As the result of salt stress mitochondria was ruined in structure and irregular solid was found to be transfered from the cytoplasm to the cell wall in root growing point.