• Title/Summary/Keyword: 비표지

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Fabrication of Label-Free Biochips Based on Localized Surface Plasmon Resonance (LSPR) and Its Application to Biosensors (국소 표면 플라즈몬 공명 (LSPR) 기반 비표지 바이오칩 제작 및 바이오센서로의 응용)

  • Kim, Do-Kyun;Park, Tae-Jung;Lee, Sang-Yup
    • KSBB Journal
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    • v.24 no.1
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    • pp.1-8
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    • 2009
  • In the past decade, we have observed rapid advances in the development of biochips in many fields including medical and environmental monitoring. Biochip experiments involve immobilizing a ligand on a solid substrate surface, and monitoring its interaction with an analyte in a sample solution. Metal nanoparticles can display extinction bands on their surfaces. These charge density oscillations are simply known as the localized surface plasmon resonance (LSPR). The high sensitivity of LSPR has been utilized to design biochips for the label-free detection of biomolecular interactions with various ligands. LSPR-based optical biochips and biosensors are easy to fabricate, and the apparatus cost for the evaluation of optical characteristics is lower than that for the conventional surface plasmon resonance apparatus. Furthermore, the operation procedure has become more convenient as it does not require labeling procedure. In this paper, we review the recent advances in LSPR research and also describe the LSPR-based optical biosensor constructed with a core-shell dielectric nanoparticle biochip for its application to label-free biomolecular detections such as antigen-antibody interaction.

Estimate of pesticide residues in tomato varieties using ratio of surface area to weight (비표면적을 이용한 토마토의 과종별 농약 잔류량 예측)

  • Kwon, Hye-Young;Kim, Jin-Bae;Lee, Hee-Dong;Ihm, Yang-Bin;Kyung, Kee-Sung;Park, In-Hee;Choi, Jung
    • The Korean Journal of Pesticide Science
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    • v.8 no.1
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    • pp.30-37
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    • 2004
  • This study estimated the correlation between surface area/weight ratios and pesticide residues in two varieties of tomato, mini tomato and tomato. The ratios of surface area/weight of mini tomato and tomato were $2.15cm^2g^{-1}$ and $1.00cm^2g^{-1}$ respectively. Pesticide residues were measured with four kinds of pesticide, chlorothalonil WP, chlorothalonil SC, oxadixyl WP, thiophanate-methyl WP. Ie residue amount of mini tomato was $1.4\sim2.4$ times higher than those in tomato regardless of the application date and frequency. The ratios of surface area/weight on tomato varieties played a key role for determining pesticide residue.

Explosion Riskiness with Flying of Carbon Black Dust by Hartman (Hartman식 장치에 의한 Carbon Black 분진의 부유중 폭발 위험성 평가)

  • 현성호;김정환;이창우
    • Fire Science and Engineering
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    • v.12 no.4
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    • pp.13-19
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    • 1998
  • We investigated the weight loss according to temperature using Thermal Gravimetric Analysis(TGA) in order to find the thermal hazard of carbon black(Hi-Black 10, Hi-Black 50L) dusts, and the properties of dust explosion in variation of the surface functional groups and specific surface area of their dust with the same particle size. Using Hartman's dust explosion apparatus which estimate dust explosion by electric ignition after making dust disperse by compressed air, dust explosion experiments have been conducted by varying concentration and size of carbon black dust. The explosion pressure of both carbon black increased as the specific surface area increased. The results indicated that Hi-Block 50L of which specific surface area was larger three to four times than that of Hi-Black 10 was much easier of dust explosion.

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Structure Control of Zinc Film Using Magnetron Sputtering (마그네트론 스퍼터링법을 이용한 Zn 나노 구조 제어)

  • Lee, Won-Ji;Yun, Seong-Ho;Kim, An-Na;Park, Jin-Seong;Kim, Hyeon-Jong;Lee, Ho-Nyeon
    • Proceedings of the Korean Institute of Surface Engineering Conference
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    • 2018.06a
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    • pp.85.1-85.1
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    • 2018
  • 고표면적 다공성 금속 박막 형성 기술은 타겟에서 방출된 금속 원자들이 타겟 주변에서 서로 충돌하여 나노입자를 형성한 후 기판으로 입자를 이송시켜 나노 구조를 지니는 박막을 성장시키는 기술이다. 고표면적 다공성 금속 박막 형성 기술로 제작한 다공성 박막은 열린 기공 구조를 지니고 있기 때문에 외부 기체의 확산이 용이하고 비표면적이 높다는 특징을 가지고 있다. 특히 금속 공기 이차전지 등의 배터리의 경우 전극의 비표면적이 성능을 결정하는 중요한 인자이므로 금속판을 사용하는 경우 대비 비표면적이 높은 나노구조를 사용할 경우 용량 증대에 유리하다. 본 연구에서는 공정 압력, 공정 파워, 타겟과 기판과의 거리, 칠러 온도 등 증착 공정 변수를 제어하여 표면적이 높은 아연 나노 구조를 형성하였다. 이를 분석하기 위해 SEM을 이용하여 미세구조 및 두께를 관찰하였으며, 박막 증착 전후의 무게를 측정하여 기공률을 계산하였다. 또한 XRD 분석을 통하여 결정성 및 결정의 크기를 확인하였다. 이렇게 제작된 고표면적 다공성 Zn 금속박막을 응용하여 아연 전지 성능 평가를 진행하였다.

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A Study on the Explosion Riskiness with Flying of Activated Carbon (활성탄의 부유중 폭발 위험성에 관한 연구)

  • 김정환;현성호;이창우;함영민
    • Fire Science and Engineering
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    • v.12 no.3
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    • pp.3-9
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    • 1998
  • We investigated the weight loss according to temperature using TGA in order to find the thermal hazard of brand-new activated-carbon and disused activated-carbon dusts, and the properties of dust explosion in variation of the specific surface area of their dust with the same particle size. Using hartman's dust explosion apparatus which estimate dust explosion by electric ignition after making dust disperse by compressed air, dust explosion experiments have been conducted by varying concentration and size of activated carbon dust. The explosion pressure of both activated carbon increased as the specipic surface area increased. The results indicated that brand-new activated-carbon of which specific surface area was larger three to four times than that of disused activated-carbon was much easier of dust explosion.

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Target/non-target classification using active sonar spectrogram image and CNN (능동소나 스펙트로그램 이미지와 CNN을 사용한 표적/비표적 식별)

  • Kim, Dong-Wook;Seok, Jong-Won;Bae, Keun-Sung
    • Journal of IKEEE
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    • v.22 no.4
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    • pp.1044-1049
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    • 2018
  • CNN (Convolutional Neural Networks) is a neural network that models animal visual information processing. And it shows good performance in various fields. In this paper, we use CNN to classify target and non-target data by analyzing the spectrogram of active sonar signal. The data were divided into 8 classes according to the ratios containing the targets and used for learning CNN. The spectrogram of the signal is divided into frames and used as inputs. As a result, it was possible to classify the target and non-target using the characteristic that the classification results of the seven classes corresponding to the target signal sequentially appear only at the position of the target signal.

The Methodology for Environmental Risk Assessments of Non-target Organisms (Insects) on LM Rice (LM벼에 영향을 받는 비표적 생물체(곤충)에 대한 환경위해성 평가 (ERA) 방법)

  • Yi, Hoonbok;Kim, Hyun-jung;Na, Sumi
    • Korean Journal of Environment and Ecology
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    • v.30 no.4
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    • pp.712-723
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    • 2016
  • This paper is to suggest the methods about the environmental risk assessment (ERA) based on non-target insect species for LM rice crop produced by biotechnology. We used some data by the picture dictionary of agricultural pests in Korea, some books for ERA or rice pest ecology, articles about rice ecology, and internet sites and many specialists gave us good advice for this ERA system. We found about 140 insect species using rice crop as their habitat and we discriminated herbivores into Sap feeder, Grain feeder, Defoliator, and Pollen feeder according to their food functional group. We also clarified the potential damage possibility of insect species from LM rice crop. Then, we ranked 10 non-target insect species (Baliothrips biformis, Laodelphax striatellus, Nilaparvata lugens, Mythimna separata, Cnaphalocrocis medinalis, Cletus schmidti Kiritshenko, Scotinophara lurida, Nilaparvata bakeri, Oxya chinensis, Agromyza oryzae) with giving some scores using Geographic distribution, Habitat specialization, Prevalence on crop, Abundance. We showed some environmental risk hypotheses about herbivore, predator, and parasitoid, and infered the impacts of non-target species on LM rice crop. Conclusively, we want to serve this methodology to build the biosafety system from biotechnology crops, and the methodology is to keep public health, sustain the biodiversity, and conserve the natural ecosystems.

RFID Mutual Autentication Protocol Using AES (AES를 이용한 RFID 상호인증 프로토콜)

  • Kim, Seok;Han, Seung-Jo
    • Journal of the Korea Institute of Information and Communication Engineering
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    • v.16 no.9
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    • pp.1963-1969
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    • 2012
  • Recently use of RFID(Radio Frequency Identification) tends to be rapidly increased and will be also extended throughout the whole life. Using radio-frequency data can be recognized automatically in the RFID system is vulnerable to personal information protection or security. And passive tags have a hardware problem is the limit for applying cryptographic. This paper presents an authentication protocol using AES and Nounce. After completing mutual authentication server to access and strengthen security vulnerability to the use of the Nounce, because safety in denial of service attacks.

Label-free Detection of Biomolecular Specific Interaction by Optical Biosensors (광 바이오센서를 이용한 비표지 생계물질들의 특이 상호작용력의 측정)

  • 김의락;최정우
    • KSBB Journal
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    • v.17 no.1
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    • pp.1-13
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    • 2002
  • Label-free optical methods for the monitoring of interactions between biological molecules have become increasingly popular within the last decade. A rising number of publications have demonstrated the benefits of direct biomolecular interaction analysis(BIA) for biology and biochemistry, such as antigen-antibody Interactions, receptor-ligand interactions, protein-DNA, DNA- intercalator, and DNA-DNA interactions. This article gives an overview of the historical development, principle and application of label-free optical biosensor to examine the functional characteristics of biospecific interaction, such as kinetics, affinity, and binding position of biomolecular between an immobilized species at the transducer surface and its dissolved binding partner.

Quantitative Alpha Fetoprotein Detection with a Piezoelectric Microcantilever Mass Sensor (압전 마이크로캔틸레버 질량센서를 이용한 정량적 알파태아단백 검출)

  • Lee, Sangk-Yu;Cho, Jong-Yun;Lee, Yeol-Ho;Jeon, Sang-Min;Cha, Hyung-Joon;Moon, Wonk-Yu
    • Journal of the Korean Society for Nondestructive Testing
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    • v.31 no.5
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    • pp.487-493
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    • 2011
  • Alpha fetoprotein(AFP), which is serological marker for hepatocellular carcinoma, was quantitatively measured by its normal concentration, 10 ng/ml, with a label-free piezoelectric microcantilever mass sensor. The principle of detection is based on changes in the resonant frequency of the piezoelectric microcantilever before and after target molecules are attached to it, and its resonant frequency is measured electrically using a conductance spectrum. The resonant frequency of the developed sensor is approximately 1.34 MHz and the mass sensitivity is approximately 175 Hz/pg. The sensor has high reliability as mass sensor by reducing the effect of surface stress on resonant frequency due to attached proteins. 'Dip and dry' technique was used to react the sensor with reagents for immobilizing AFP antibody on the sensor and detecting AFP antigen. The measured mass of the detected AFP antigen was 6.02 pg at the concentration of 10 ng/ml, and 10.67 pg at 50 ng/ml when the immunoreaction time was 10 min.