• Analytical Science and Technology
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• v.14 no.5
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• pp.384-391
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• 2001

In this study, a new quantitative analytical method has been developed for the rapid determination of acylcarnitines and amino acids in human blood using electrospray ionization / tandem mass spectrometry (ESI-MS/MS). Acylarmitines and amino acids were analyzed by tandem mass spectrometry after conversion to their butylesters through treatment with 3N butanolic hydrogen chloride. Acylcrnitines were analyzed using precursor 85 ion scan and alanine, valine, leucine/isoleucine, methionine, phenylalanine, tyrosine, aspartic acid and glutamic acid were analyzed using neutral loss 102 scan, ornitine and citrulline were analyzed neutral loss 119 scan, glycine was analyzed using neutral loss 56 scan, arginine was analyzed using neutral loss 161 scan and argininosuccinic acid was analyzed product ion 459 scan. This method reduced sample preparation time compared to that with conventional amino acid analyzer and liquid chromatography, with high sensitivity and good reproducibility.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.19 no.3
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• pp.489-498
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• 2018

This study was conducted to investigate the effect of walking exercise with blood flow restriction (BFR) on insulin resistance, adipokines and gut hormones in middle aged obese women. Eleven obese women (BMI > 25kg/m2; body fat > 30%) wore pneumatic pressure belts at both femurs and performed walking exercise consisting of walking for 2 min and resting for 1min twice per day, 3 days/wk for 4 weeks. Insulin significantly increased after exercise (p<0.05), while glucose increased slightly, but not significantly. Additionally, HOMA-IR decreased significantly after exercise (p<0.05), while adiponectin and visfatin increased, but not significantly. Finally, ghrelin (p<0.05) and GLP-1 (p<0.05) increased significantly after exercise. These results suggest that walking exercise with blood flow restriction for 4 weeks improves insulin resistance, adipokines, and gut hormones in obese middle-aged women. Therefore, high intensity walking exercise with blood flow restriction for short periods of time has more positive effects on prevention and treatment of obesity-related metabolic diseases.

• Journal of Hospice and Palliative Care
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• v.17 no.1
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• pp.18-26
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• 2014

Purpose: Anorexia is a common symptom in terminal cancer patients. Some data have suggested a role of visfatin in regulating feeding behavior. We studied the relationship of a serum visfatin level and anorexia in cancer patients provided with hospice care. Methods: After informed consent was obtained, 69 cancer patients over 20 years old at a hospice center were enrolled in the study from July 2009 to July 2010. We characterized patients by age, sex, body mass index, primary cancer site, and Eastern Cooperative Oncology Group (ECOG) performance status, etc. Also, blood tests were performed to measure the level of hemoglobin, white blood cell (WBC), C-reactive protein (CRP), total cholesterol, albumin, lymphocyte count, glucose, blood urea nitrogen (BUN), creatinine, tumor necrosis factor-alpha (TNF-${\alpha}$), interukin-6, leptin and visfatin. Results: The mean age of subjects was 65.5 years old, According to univariate analysis, pulse, ECOG performance scale, opioid use and visfatin level were different between the anorexia and non-anorexic group (P<0.05). The median visfatin level was higher in the anorexia group than the good appetite group (P=0.0323). Serum visfatin concentrations were not related with either body mass index or serum leptin or total cholesterol levels, but it was negatively correlated with serum albumin levels (P=0.0198) and lymphocyte counts (P=0.0013). Conclusion: This study did not identify a novel link between visfatin levels and anorexia in cancer patients at a hospice.