• Title/Summary/Keyword: 불활성화

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Mutagenic Deactivation of 7, 12-Dimethylbenz(a)anthtacene in Nonacclimated Soil (불순응된 토양에서 이메틸벤조안트라센 돌연변이 유발성의 불활성화)

  • 임동준;박갑성
    • KSBB Journal
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    • v.4 no.1
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    • pp.8-10
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    • 1989
  • Mutagenic characteristics deactivation of 7, 12-dimethylbenz(a)anthraracene was studied in a nonacclimated sandy loam soil at low and neutral pH soil conditions. Soil extracts containing transformation products were separated into three fractions based on HPLC retention time(polarity). Highly polar transformation products of 7, 12-dimethylbenz(a)anthracene demonstrated a negative mutagenic response with the Ames mutagenicity assay, strain TA-100, for both low and neutral pH soils. Moderate and low polar fractions, however, induced mutagenicity for both soil samples with mutagenic ratios similar to those of the parent compound.

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Stabilization of Rice Bran by Microwave Energy (마이크로파 에너지에 의한 쌀겨의 안정화)

  • Rhee, Joon-S.;Yoon, Heeny H.N.
    • Korean Journal of Food Science and Technology
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    • v.16 no.1
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    • pp.113-119
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    • 1984
  • Initial moisture content and weight of the rice bran and treatment time were identified as important variables for the inactivation of lipase and peroxidase present in rice bran. Multiple regression analysis was used to obtain a prediction equation to measure the effects of moisture content and weight of the sample and microwave treatment time on the residual lipase activity and loss in weight. It was found that the microwave treatment did not affect acid value and extractability of the rice bran oil.

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Isolation and Thermal Inactivation of Horseradish Peroxidase Isozymes (서양고추냉이에 있는 페르옥시다아제 이소짐의 분리(分離)와 열불활성화(熱不活性化))

  • Yoon, Jung-Ro;Park, Kwan-Hwa
    • Korean Journal of Food Science and Technology
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    • v.14 no.2
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    • pp.125-129
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    • 1982
  • Four peroxidase isozymes from horseradish roots (isozymes A, B, C and D) were isolated by chromatography and were thermally inactivated at $70{\sim}97^{\circ}C$ and pH 7.0. The four isozymes had different inactivation rates and the inactivation of each isozymes did not follow first order kinetics. D values of isozymes A, B, C, D and crude enzyme were 594s, 1850s, 2050s, 78s, 130s and z values were $24.0^{\circ}C$, $12.5^{\circ}C$, $18.0^{\circ}C$, $23.7^{\circ}C$ and $24.0^{\circ}C$, respectively. Sephadex gel chromatogram of the thermally treated isozyme C indicated that the shape and molecular weight of the native isozyme changed during inactivation.

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Control of Bacterial Adhesion and Biofilm Using Electric Field (전기장을 이용한 미생물 부착과 생물막 제어)

  • Shim, Soo-Jin;Kim, Choon-Soo;Yoon, Je-Yong
    • Journal of Korean Society of Environmental Engineers
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    • v.33 no.9
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    • pp.692-700
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    • 2011
  • The use of electric field has been studied as an alternative for biofilm control dominated by disinfectants and antibiotics. This technology would be advantageous in the environmental respect that biofilm can be controlled based on electron transfer, not using chemical disinfectants and antibiotics. Control mechanisms which were reported by earlier studies are organized as; (1) bacterial adhesion control by electrostatic repulsion at a negative current, (2) bacterial adhesion control using bacterial motion and (3) bacterial inactivation by direct oxidation at a positive current, (4) bioelectric effect leading to biofilm inactivation. In this review article, we summarized the technologies for biofilm control using electric field and provided some application examples from previous studies.

High Pressure Inactivation of Alliinase and Its Effects on Flavor of Garlic (고압처리에 의한 Alliinase의 불활성화가 마늘의 풍미에 미치는 영향)

  • Sohn, Kyung-Hyun;Lim, Jae-Kag;Kong, Un-Young;Park, Ji-Yong
    • Korean Journal of Food Science and Technology
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    • v.28 no.3
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    • pp.593-599
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    • 1996
  • The effects of high pressure on alliinase and on flavor of garlic (Alliiium sativum L.) were investigated. After pressurized at 150 MPa, 300 MPa, and 500 MPa for 10 min, the activities of purified alliinase were reduced approximately 30%, 80%, and 100%, respectively, while the enzyme activities of pressurized garlic cloves were reduced 0%, 7%, and 100%, respectively. This indicated that the intact garlic has a protective effect against pressure-inactivation of alliinase. Alliinase was more effectively inactivated when high pressure treatment was carried out at high ($>40^{\circ}C$) or low temperature ($>10^{\circ}C$) than ambient temperature. Pressure treated garlic at 500 MPa had little pungency and sulfuryl odor compared to raw garlic indicating that high-pressure processing can be used to produce garlic without pungent flavor.

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Effect of Hydrogen Ratio and Tin Addition on the Coke Formation of Platinum Catalyst for Propane Dehydrogenation Reaction (프로판 탈수소화 반응용 백금촉매의 코크 생성에 미치는 수소비와 주석첨가의 영향)

  • Kim, Soo Young;Kim, Ga Hee;Koh, Hyoung Lim
    • Clean Technology
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    • v.22 no.2
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    • pp.82-88
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    • 2016
  • The loss of activity by coke is an important cause of catalyst deactivation during industrial operation. In this study, hydrogen ratio of reaction condition, which has influenced on coke formation over Pt-Sn catalyst, and regeneration of catalysts activity by coke burning, Pt sintering of coke burning as coke contents, effects of coke formation and deactivation with different Sn contents were confirmed. Pt-Sn-K catalyst supported on θ-alumina and γ-alumina was prepared progressively. Activity of regenerated catalyst for propane dehydrogenation was compared with fresh catalyst by coke burning, after propane dehydrogenation was carried out with different hydrogen ratio at 620 ℃ on fresh catalyst. Regenerated catalyst’s physical characterization such as BET, coke analysis and XRD was investigated. Through catalytic activity test and characterization, Sn contents of catalyst and hydrogen ratio in feed stream could affect coke formation on catalyst surface. Excessive coke makes loss of activity and Pt sintering during air regeneration process.

Detection of Poliovirus in Water by Cell Culture and PCR Methods (세포배양법과 PCR 방법에 의한 물에서의 폴리오 바이러스 검출)

  • 조연희;이찬희
    • Korean Journal of Microbiology
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    • v.38 no.3
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    • pp.198-204
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    • 2002
  • Poliovirus is a member of enterovirus which causes paralytic poliomyelitis, encephalitis and aseptic meningitis. Since poliovirus is spread by the fecal-oral route and poliovirus-contaminated water could be a potential threat for public health, detection of poliovirus in drinking water resource is important. Infectious poliovirus and poliovirus inactivated by heat or UV were used to test three detection methods such as cell culture method, reverse transcription-polymerase chain reaction (RT-PCR) and integrated cell culture (ICC)-PCR. Infectious poliovirus was detected by all three methods and ICC-PCR was the most sensitive and fast in detecting poliovirus. Inactivated polioviruses could not be detected by cell culture or ICC-PCR methods. On the other hand, heat- inactivated viruses could be detected by RT-PCR. Thus it is suggested that ICC-PCR method is the most sensitive and effective in detecting infectious polioviruses in water sample.

Antimicrobial Activity and Mechanism of Supercritical Fluids (초임계 유체의 미생물 불활성화 특성 및 기작)

  • Mun, Sungmin;Kim, Jungchan;Lee, Youn-Woo;Yoon, Jeyong
    • Applied Chemistry for Engineering
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    • v.22 no.5
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    • pp.453-460
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    • 2011
  • Recently, there is growing interests in the application of supercritical fluids for food and medical fields since supercritical fluids ($CO_2$ and $N_2O$) have known to be safe and effective as a non-thermal sterilization technique. Although supercritical fluids have been investigated for various kinds of products, they have not yet been used in common currency due to their lack of knowledge related to the antimicrobial activity or detailed mechanisms. In this review paper, we summarized the characteristics, antimicrobial activity and mechanisms, important factors, and applicability of supercritical fluids to help the investigation and commercialization of supercritical fluids sterilization technique.

Effect of Microwave Vacuum Heating on Inactivation of Enzymes (마이크로파 진공가열방법이 효소의 불활성화에 미치는 영향)

  • Moon, Eun-Kyung;Han, Ki-Young;Kim, Suk-Shin;Kim, Sang-Young;Noh, Bong-Soo
    • Korean Journal of Food Science and Technology
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    • v.29 no.2
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    • pp.284-291
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    • 1997
  • Microwave vacuum heating method (2450 MHz) was used for a low intensity of heat treatments. High vacuum under the microwave heating could bring low temperature condition. Inactivation of ${\alpha}-amylase,\;{\beta}-amylase$, glucoamylase and peroxidase by microwave vacuum heating were investigated at 60-$80^{\circ}C$. It was compared with conventional heating. The heating condition of microwave vacuum heating was confirmed by the destruction of ascorbic acid. When thermal inactivations of the enzymes by microwave vacuum heating were determined, it was less effective than that of conventional method at the initial stage of heating. It was due to a lag time of microwave heating. However, the heating time for complete inactivation of the enzymes by microwave vacuum heating could be reduced comparing with that of conventional heating. Optimum conditions for inactivation of the enzymes could be obtained by microwave vacuum heating.

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Synergistic Effect of Grapefruit Seed Extract, EDTA and Heat on Inactivation of Bacillus cereus Spore (자몽종자추출물, EDTA와 열 병행에 의한 Bacillus cereus 포자 불활성화 상승효과)

  • Yang, Seung-Kuk;Kim, Jung-Jee;Kim, Seok-Joong;Oh, Se-Wook
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.40 no.10
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    • pp.1469-1473
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    • 2011
  • The efficacy of antimicrobial agents and heat treatments on spore inactivation was investigated. Grapefruit seed extract (GFE) and ethylenediaminetetraacetic acid (EDTA) were used and as antimicrobial agents, and heat treatments were conducted at $70^{\circ}C$, $80^{\circ}C$, and $90^{\circ}C$ for 30 minutes. Heat treatments at $90^{\circ}C$ were the most effective on spore inactivation as a single treatment and caused a 2.3 log reduction. When combined with a single treatment to discover synergistic effects, 1% GFE with $80^{\circ}C$ heat treatments and 0.5 mM EDTA with $80^{\circ}C$ heat treatments resulted in 2.1 log and 3.2 log reductions, respectively, though they did not show reductions at each single treatment (GFE 1% (v/v), EDTA 0.5 mM, $80^{\circ}C$). So it was concluded that by combining GFE, EDTA in low concentration treatment, and heat treatment, B. cereus spores can be effectively inactivated.