• Title/Summary/Keyword: 분화소재

Search Result 195, Processing Time 0.035 seconds

Inhibitory Effects of Allium senescens L. Methanol Extracts on Reactive Oxygen Species Production and Lipid Accumulation during Differentiation in 3T3-L1 Cells (두메부추(Allium senescens L.) 메탄올 추출물의 지방세포 내 활성산소종 생성 및 지질축적 억제 효능)

  • Choi, Hye-Young;Kim, Gun-Hee
    • Korean Journal of Food Science and Technology
    • /
    • v.46 no.4
    • /
    • pp.498-504
    • /
    • 2014
  • Allium senescens L. is perennial plant of the Liliaceae family that grows throughout Korea. In this study, we investigated the effect of Allium senescens L. methanol extracts on reactive oxygen species (ROS) production and lipid accumulation during adipogenesis. Our results indicated that 1,1-diphenyl-2-picrylhydrazyl radical scavenging activity of Allium senescens L. methanol extracts increased in a dose-dependent manner. Allium senescens L. methanol extracts suppressed ROS production and lipid accumulation during adipogenesis. In addition, Allium senescens L. methanol extracts inhibited the mRNA expression of the pro-oxidant enzyme, such as G6PDH and lead to a reduction in the mRNA levels of the transcription factors, such as sterol regulatory element binding proteins 1c, peroxisome proliferator-activated receptor ${\gamma}$, and CCAAT/enhancer-binding proteins ${\alpha}$. These results indicate that Allium senescens L. methanol extracts inhibit adipogenesis by modulating ROS production associated with ROS-regulating genes and directly down-regulating adipogenic transcription factors.

Anti-Obesity Activity of Euptelea Pleiosperma Ethanol Extract (Euptelea pleiosperma 에탄올 추출물의 항비만 활성)

  • Park, Jung Ae;Jin, Kyong-Suk;Kwon, Hyun Ju;Kim, Byung Woo
    • Microbiology and Biotechnology Letters
    • /
    • v.43 no.4
    • /
    • pp.336-342
    • /
    • 2015
  • Previously, Euptelea pleiosperma was identified as one of the useful sources containing anti-oxidative and anti-inflammatory activities for the first time in our research group. In this study, anti-obesity effect of E. pleiosperma ethanol extract (EPEE) was evaluated by using a pancreatic lipase enzyme inhibition assay and a cell culture model system. EPEE suppressed effectively pancreatic lipase enzyme activity dose dependently. Furthermore, EPEE significantly suppressed adipocyte differentiation, lipid accumulation, triglyceride contents, and triggered lipolysis activity on 3T3-L1 preadipocytes in a dose-dependent manner without cytotoxicity. Anti-adipogenic effect of EPEE was modulated by cytidine-cytidine-adenosine-adenosine-thymidine (CCAAT)/enhancer binding proteins ${\alpha}(C/EBP{\alpha})$, $C/EBP{\beta}$ and peroxisome proliferator-activated receptor ${\gamma}(PPAR{\gamma})$ gene and protein expressions. Taken together, these results provide the important new insight that E. pleiosperma possesses anti-obesity activities such as pancreatic lipase inhibition, anti-adipogenic, and lipolysis effects. It might be utilized as promising sources in the fields of nutraceuticals. The identification of active compounds that confer anti-obesity activity of EPEE might be needed.

Antioxidant and Anti-adipogenic Effects of Kohlrabi and Radish Sprout Extracts (콜라비 새싹 추출물과 무순 추출물의 항산화 및 지방세포 분화 억제 활성)

  • Lee, Young-Jun;Kim, Jae-Hwan;Oh, Ji-Won;Shin, Gi-Hae;Lee, Jong Seok;Cho, Ju-Hyun;Park, Jin-Ju;Lim, Jeong-Ho;Lee, Ok-Hwan
    • Korean Journal of Food Science and Technology
    • /
    • v.46 no.5
    • /
    • pp.531-537
    • /
    • 2014
  • Common cruciferous vegetables, kohlabi (Brassica oleracea) and radish (Raphanus sativus), contain phytochemicals such as glucosinolates and carotenoids. Therefore, this study investigated the antioxidant and anti-adipogenic effects of kohlrabi sprout extract (KSE) and radish sprout extract (RSE). The total carotenoid and glucosinolate contents of KSE and RSE were $39.50{\pm}0.67$ and $76.73{\pm}2.75mg/g$, respectively. The total glucosinolate contents of KSE and RSE were $2.65{\pm}0.02$ and $8.13{\pm}0.54mg/g$, respectively. The in vitro-antioxidative activities of KSE and RSE were significantly increased in a dose-dependent manner. Furthermore, ${\beta}$-carotene and glucosinolate-enriched KSE and RSE significantly inhibited lipid accumulation and reactive oxygen species production during the adipogenesis of 3T3-L1 preadipocytes. These results suggest that glucosinolate-enriched KSE and RSE, especially RSE, can be used in the treatment of obesity and as a natural source of antioxidants.

Several Treatments of Veronica rotunda Nakai for Pot Cultivation (분화 재배를 위한 둥근산꼬리풀의 몇 가지 처리)

  • Yeon, Soo Ho;Lee, Sang In;Lee, Seung Yeon;Lee, Cheol Hee
    • Proceedings of the Plant Resources Society of Korea Conference
    • /
    • 2019.04a
    • /
    • pp.89-89
    • /
    • 2019
  • 둥근산꼬리풀(Veronica rotunda Nakai)은 다년생 초본으로 높은산 정상에서 자라며, 초장은 10~20 cm, 잎의 길이는 2~4 cm 정도이다. 또한, 기부의 잎은 엽병이 불분명하고, 줄기에는 털이 없는 것으로 알려져 있다. 관상소재로 이용되고 있으나, 식물정보는 매우 부족하며, 활용하기 위해서는 재배 연구와 식물에 대한 정보 수집이 필요하다. 이에 본 연구는 둥근산꼬리풀의 관상용 재배 시 적정 차광, 기비, 추비, 왜화제 및 적심 등의 처리 정도를 알아보고자 수행하였다. 재배방법의 확립을 위하여 선행연구에서 육묘한 유묘(2018년 5월 15일에 파종하여 생산)를 7월 16일에 정식하여 10월 15일까지 약 14주 동안 재배하였다. 공통조건은 플라스틱 화분 10호(이색포트, $9{\times}9cm$)에 원예상토를 충진하고, 200구 트레이에 셀 당 4립씩 파종하여 생산된 1셀을 정식하였다. 차광 정도 실험은 0, 55, 75% 차광막을 이용하였으며, 기비는 싱싱코트를 0, 10, 20알 처리하였고, 추비는 hyponex (N:P:K, 4:6:6)를 0, 1000, $2000mg{\cdot}L-1$의 농도로 엽면시비 하였다. 또한, 관상효과 증가를 위해 왜화제는 diniconazole, daminozide를 농도별(각 0, 1000, $2000mg{\cdot}L-1$)로 처리하였고, 적심은 4주차에 1회 처리 유무의 효과를 비교하였다. 연구의 결과, 둥근산꼬리풀에 기비 10알 처리는 생육에 유의적인 차이는 보이지 않았으나, 20알 처리는 무처리와 10알 처리에 비해 절간장, 경직경이 증가하는 결과가 나타났다. 추비는 절간장을 증가시켰으나, 측지수와 마디수는 유의적으로 감소시켰다. 차광처리별로는 55, 75% 차광 모두 무차광에 비해 측지수, 마디수, 엽수 및 개화율을 크게 감소시키는 결과를 보여, 둥근산꼬리풀는 광이 충분한 곳에서 재배하는 것이 적합한 것으로 생각된다. 왜화제의 처리는 종류와 농도에 관계없이 둥근산꼬리풀은 측지수, 마디수, 엽수를 감소시키는 결과를 보였다. 또한 초장이 짧아지고 경직경은 두꺼워졌으나, 유의적인 차이는 없었다. 종류별로는 daminozide $2000mg{\cdot}L-1$ 처리는 무처리에 비해 개화율이 증가하여, 둥근산꼬리풀의 분화용 재배시 daminozide $2000mg{\cdot}L-1$를 처리하는 것이 효과적이다. 적심처리는 무처리에 비해 전반적인 생육 및 개화를 억제하는 결과가 나타났다. 결론적으로 둥근산꼬리풀의 분화용 재배시 광량이 충분한 곳에서 재배하고, 기비와 추비는 절간장을 증가시키며, 측지수, 마디수, 엽수에 큰 차이가 없거나 감소하는 결과를 보여 관상용 재배에 적합하지 않은 것으로 판단된다. 추가적으로 daminozide $2000mg{\cdot}L-1$를 처리하며, 적심처리를 하지 않는 것이 좋을 것으로 생각된다.

  • PDF

Feature and Operation on Correlation for Royal (State operation) Storage of Baekjae (백제 왕실(국영) 창고시설의 특징과 운영)

  • So, Jae Yun
    • Korean Journal of Heritage: History & Science
    • /
    • v.45 no.4
    • /
    • pp.22-37
    • /
    • 2012
  • Storage facility of the pre-historic Korea is classified into the subterranean, ground and overground types. The rectangular-shaped subterranean Storage facilities have been identified in the Pungnaptoseong, the Gong mountain fortress site and the Gwanbukri site. Feature no. 196 at Gyeongdang District in Pungnaptoseong yielded a large quantity of glazed potteries, and a wooden storage at the Gwanbukri site contained a large amount of fruit seeds. These storage facilities might be functioned as the warehouse for the highest group rather than the storage for the emergency such as war and flooding and stipend of government officials. This article subdivided into "state storage" on the concept of the former that "royal storage" on the concept of the latter. If it look on the state storage at large, this include the royal storage too. But it subdivided to help article understand because Baekjae changed from state storage to royal storage by change and specialization of system after 4th. The reason why the diversification of storage pits was closely related to the unification of local polities and the concentration of political power in the state-level. Therefore, it might reflect the political circumstances the ruling elites attempted to heighten their authority in terms of the organizing tax collecting system. And divided the time of storage is confirmed separative storage pits in the suburbs of capital city. There is hight probability of top local polities or nation that have possessional a role. This is to cover on frequent war in the Three States. On the other hand, state storage is located around ancent road that linked castle gate that is divided into center and periphery depending on function and position of storage. Center is located royal storage focusing in the presumed royal palace that periphery is located state storage to provide service to the public. It is presumed that located with the government office.

Effects of Magnolia Officinalis Bark Extract on Improvement of Lip Wrinkles (요엽후박나무 추출물의 입술 주름 개선에 대한 연구)

  • Lee, Seonju;Kim, Mina;Park, Sung Bum;Kim, Ki Young;Park, Sun-Gyoo;Kim, Mi-Sun;Kang, Nae-Gyu
    • Journal of the Society of Cosmetic Scientists of Korea
    • /
    • v.45 no.1
    • /
    • pp.95-103
    • /
    • 2019
  • Lips have a defect in maintenance of moisture due to their thin layer. As aging progresses, lips lose volume and redness, and become wrinkled. Fat grafting and filler surgery have been used to achieve attractive lips, but little research has been reported to develop better materials to replace the present methods. Recently, a study suggests that the increase of adipocyte number can be enhancing the expansion endogenous fat. In previous study, we identified that the efficacy of Magnolia officinalis bark extract (MOBE) was effective on the induction of adipogenic differentiation. In this study, we confirmed that MOBE enhanced the differentiation of human adipose-derived stem cells on the fat mimic 3D structure built by 3D bioprinting method From further experiments in human, we established a method to quantify the severity of lip wrinkle by measurement of standard deviation of gray value using Image J software. Finally, we found that topical treatment with 1% MOBE formulated lip balm significantly improved the lip wrinkle after using for 12 weeks. In conclusion, these findings suggest that MOBE has great potential, as a cosmetic ingredient, to reduce the lip wrinkle through the effect of promoting adipogenic differentiation.

Effects of mulberry fruit juice powder on inflammation and microRNA-132/143 regulation in 3T3-L1 adipocytes (뽕나무 열매 착즙 분말이 3T3-L1 지방세포의 염증 및 microRNA-132/143 조절에 미치는 영향)

  • Lee, Mak-Soon;Kim, Yangha
    • Journal of Nutrition and Health
    • /
    • v.54 no.5
    • /
    • pp.448-458
    • /
    • 2021
  • Purpose: Mulberry (Morus alba L.) fruit is widely grown in Asia and consumed as fresh fruit, jam, and juices. The fruit has beneficial health effects, including anti-diabetic, anti-tumor, and anti-obesity properties. However, the mechanisms by which mulberry fruit juice powder (MJ) regulates inflammatory microRNAs (miRs) are not yet known. This study investigated the effect of mulberry fruit juice powder on the regulation of inflammation and miR-132/143 during 3T3-L1 adipocyte differentiation. Methods: The 3T3-L1 cells were induced to differentiate for 2 days and then treated with various concentrations of MJ for 7 days. Cytotoxicity was determined by evaluating cell viability using a water-soluble tetrazolium salt-8 assay kit. Intracellular lipid accumulation was evaluated by oil-red O staining. The levels of the expression of genes involved in adipogenesis and inflammation, and miR-132/143 were measured by quantitative real-time polymerase chain reactions. Results: MJ showed no cytotoxic effect on 3T3-L1 adipocytes at concentrations below 100 ng/mL. Intracellular lipid accumulation was reduced by MJ treatment at concentrations of 100 ng/mL. The messenger RNA (mRNA) levels of proliferator-activated receptor-γ, cytosine-cytosine-adenosine-adenosine-thymidine/enhancer-binding protein-α, and adipocyte protein 2, which are involved in adipogenesis, were suppressed by MJ. A reduction was also seen in mRNA levels of genes related to the inflammatory response, such as tumor necrosis factor-α, interleukin-6, monocyte chemoattractant protein-1, and inducible nitric oxide synthase. The expression of the inflammatory miR-132 and miR-143 was also decreased by MJ. Conclusion: These results suggest that MJ may suppress adipogenesis and inflammation through the regulation of miR-132/143 expression in 3T3-L1 adipocytes. Thus, MJ may be useful as a food agent that prevents obesity-associated inflammation.

Effects of dietary Gelidium elegans extract on fat metabolism in preadipocyte cell and mice fed a high-fat diet (개별인정원료 우뭇가사리추출물의 체지방 감소에 관한 기능성 고찰)

  • Lee, Boo-Yong;Chung, Hee-Chul
    • Food Science and Industry
    • /
    • v.53 no.4
    • /
    • pp.390-396
    • /
    • 2020
  • We investigated the anti-obesity effect of Gelidium elegans extract (GE) on 3T3-L1 preadipocytes and a high-fat-diet (HFD)-induced mouse model. The results of the present study demonstrated that GE prevents weight gain induced by a high-fat diet (HFD) by modulating the adenosine monophosphate-activated protein kinase (AMPK)-PR domain-containing 16 (PRDM16)-uncoupling protein-1 (UCP-1) pathway in a mice model. Moreover, in vitro results show that GE suppressed adipocyte differentiation by modulating adipogenic regulators, stimulated lipolysis by activating ATGL, and inhibited adipogenesis by downregulating various enzymes associated with triglyceride synthesis. GE was also found to upregulate AMPK phosphorylation as well as the expression of UCP1 and PRDM16 proteins, leading to measurable changes in the beige-like phenotype differentiation of 3T3-L1 cells. Taken together, these findings suggest the role of GE as a functional food ingredient extracted from Gelidium elegans to increase energy expenditure and anti-obesity efficacy.

Transcriptomic Analysis of the Difference of Bovine Satellite Cell Between Longissimus dorsi and Semimembranosus on Hanwoo Muscle Tissues (한우의 등심과 사태조직 유래 근육위성세포의 성장단계별 유전발현 차이 분석)

  • Kim, H.J.;Kang, D.H.;Park, B.H.;Lee, W.Y.;Choi, J.H.;Chung, K.Y.
    • Journal of Practical Agriculture & Fisheries Research
    • /
    • v.23 no.1
    • /
    • pp.117-128
    • /
    • 2021
  • The skeletal muscle development of Hanwoo steer has been processed in the prenatal and postnatal periods. Bovine satellite cell located in perimysium of muscle tissues has differentially distributed in peripheral tissues. The study of postnatal development of satellite cells can help understand the genetic and functional regulation of meat characteristics. Factors affecting muscle size increase are related to the accumulation of DNA or synthesis of RNA proteins. In this study, we observed muscle development and differentiation after culturing bovine satellite cells derived from longissimus dorsi and semimembranosus regions of Hanwoo muscle tissue. In addition, RNA sequencing data were analyzed for differentially expressed genes (DEG) involved in intracellular muscle development and growth. The DEG of the two muscle tissues were compared according to 1day, 2day, 4day, and 7day. The overall gene expression level was confirmed by the heat map. Gene Ontology (GO) classification method was used to compare the expression level of gene groups affecting LD and SM development. The histology of GO was consistent with the time-cause change of LD and SM cell morphology. SM showed more active skeletal muscle development than LD. Even within the same time, SM expressed more genes than LD, thus synthesizing more muscle fibers

Antioxidant and Anti-obesity Effects of Herbal Complex Extract (한방복합추출물의 항산화 및 항비만 효과)

  • Shim, Jae-Won;Lee, Seung-Ju;Kim, Hye Kyung;Choi, Yun-Sik;Jang, Young-Ah
    • Journal of Life Science
    • /
    • v.32 no.7
    • /
    • pp.523-531
    • /
    • 2022
  • The purpose of this study was to evaluate the effect of antioxidant and anti-adipogenic activities in ethanol extracts from herb mixture (Ephedra sinica, Atractylodes lance, Gypsum fibrosum, and Theobroma cacao). DPPH, ABTS+ radical and xanthine oxidase scavenging activities were measured for antioxidant activity. Extracts of the herb mixture had 75.0, 100.8, and 79.5% scavenging activities at 1,000 ㎍/ml concentration, respectively. We investigated the inhibition of adipogenesis and adipocyte differentiation with an extract of an herb mixture in 3T3-L1 preadipocytes. An extract from the herb mixture at concentrations between 0 and 50 ㎍/ml did not affect 3T3-L1 cell viability. Treatment with herb mixture extracts of 25, 50, and 75 ㎍/ml in 3T3-L1 preadipocytes inhibited lipid accumulation in a dose-dependent manner. As a result of a Western blot experiment, it was shown that the herb mixture inhibited the differentiation transcription factors, PPARγ and C/EBPa, by 44.2 and 77.6%, respectively, at a concentration of 75 ㎍/ml in MDI-induced differentiated 3T3-L1 cells. As a result of RT-PCR, the gene expression of C/EBPa, SREBP-1c, and PPARγ was significantly inhibited by 43.4%, 59.6%, and 55.3%, respectively, at the concentration of 75 ㎍/ml of the herb mixture compared with the MDI-treated group. In addition, the expression of fatty acid synthase (FAS), a fatty acid synthesis regulator, was suppressed. These results can be applied to develop a functional food for anti-obesity with a herb mixture.