• The Science & Technology
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• no.1 s.404
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• pp.12-13
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• 2003

미생물 조작 새로운 생명체 창조/ 병을 따지 않고도 포도주 맛 감지/ 나노튜브를 분자 단위로 용접/ 초전도체의 원자 구조 영상/ 다이아몬드 칩 제조 방법 개발/ 쥐 유전자 99%가 인간과 비슷/ 목성의 위성 아말데아에 많은 구멍/ 의대에서 살아있는 동물 실험 줄어든다

• Nuclear Medicine and Molecular Imaging
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• v.41 no.3
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• pp.226-233
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• 2007

Purpose: Dual reporter gene imaging has several advantages for more sophisticated molecular imaging studies such as gene therapy monitoring. Herein, we have constructed hepatoma cell line expressing dual reporter genes of sodium iodide symporter (NIS) and enhanced green fluorescence protein (EGFP), and the functionalities of the genes were evaluated in vivo by nuclear and optical imaging. Materials and Methods: A pRetro-PN vector was constructed after separating NIS gene from pcDNA-NIS. RSV-EGFP-WPRE fragment separated from pLNRGW was cloned into pRetro-PN vector. The final vector expressing dual reporter genes was named pRetro-PNRGW. A human hepatoma (HepG2) cells were transfected by the retrovirus containing NIS and EGFP gene (HepG2-NE). Expression of NIS gene was confirmed by RT-PCR, radioiodine uptake and efflux studies. Expression of EGFP was confirmed by RT-PCR and fluorescence microscope. The HepG2 and HepG2-NE cells were implanted in shoulder and hindlimb of nude mice, then fluorescence image, gamma camera image and I-124 microPET image were undertaken. Results: The HepG2-NE cell was successfully constructed. RT-PCR showed NIS and EGFP mRNA expression. About 50% of cells showed fluorescence. The iodine uptake of NIS-expressed cells was about 9 times higher than control. In efflux study, $T_{1/2}$ of HepG2-NE cells was 9 min. HepG2-NE xenograft showed high signal-to-background fluorescent spots and higher iodine-uptake compared to those of HepG2 xenograft. Conclusion: A hepatoma cell line expressing NIS and EGFP dual reporter genes was successfully constructed and could be used as a potential either by therapeutic gene or imaging reporter gene.

• Journal of the Korean earth science society
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• v.31 no.4
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• pp.370-377
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• 2010

In this study, we investigated the kinematical properties of the L1521F-IRS in Taurus region using HCN (J=1-0) molecular line. The high resolution mapping has carried out by $5{\times}5$ point observations covering $3.7'{\times}3.7'$ area using a 12-m telescope of Arizona Radio Observatory in Tucsan, USA. L1521F which harbors the faint infrared L1521F-IRS, displayed a strong central concentration of integrated intensity in HCN without serious molecular depletion. It showed a symmetric kinematical structure with the opposite infall motion in either side of the central cores. It is a direct evidence of bipolar outflows in the core of L1521F.

• Analytical Science and Technology
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• v.15 no.3
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• pp.229-235
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• 2002

Plasma source ion implantation (PSII) technique was utilized to improve the wettability of polystyrene surfaces. It is well known that treated surfaces undergo aging, leading to hydrophobic recovery with time. We investigated the aging effect of polystyrene thin film on the various molecular weights. Polystyrenes with several molecular weights ($M_w$ = 760, 2430, 31600, 115700, 280000, 903600) were treated in different experimental conditions including gas species and pulse energy, and their hydrophilicity was measured by contact angle goniometer. To study wettability decay as a function of the molecular weight, PSII-treated samples were aged at different temperatures. Hydrophobic recovery of high molecular weight polystyrene was much slower than that of low molecular weight, even at high temperatures. The methods used to characterize treated surfaces were water contact angle measurement, TOF-SIMS, XPS, SEM and AFM.

• Applied Chemistry for Engineering
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• v.28 no.2
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• pp.141-152
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• 2017

Inverse electron-demand Diels-Alder reactions (IEDDA) between tetrazine derivatives and strained dienophiles have attracted a lot of attention for the efficient conjugation of biomolecules, polymers, and nanomaterials. Excellent specificity, exceptionally fast reaction rate, and biocompatibility are key features of IEDDA. Therefore, it has also been applied to the development of new labeling methods using several radioisotopes and development of radiotracers to carry out various nuclear imaging as well as therapeutic studies. The purpose of this review is to introduce the reader to the recent advances and applications of IEDDA in the fields of radiochemistry and nuclear medicine.

• Proceedings of the KSMRM Conference
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• 1999.04a
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• pp.1-9
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• 1999

핵자기 공명(NMR:nuclear magnetic resonance)은 19451건 Bloch, Procell등에 의해 처음으로 실험적으로 입증된 후에 생화학 및 약학 분야에서 분자단위의 물질의 성분, 구조, 대사물질의 역학적 운동 및 상태에 관한 정보를 아는데 유용한 기법으로 사용 되어 왔다. 의학분야에서는 Lauterber등에 의해 자기공명영상볍(Magnetic Resonance Imaging:MRI)으로 개발되어 질병의 진단 및 치료에 많은 공헌을 하게되었다. 해부학적영상 뿐 아니라 분석방법으로 병변 부위 및 인체 기관에서의 각종 대사물질의 정량적인 양과 변화를 알 수 있는 자기공명분광기법(magnetic resonance spectroscopy:MRS)도 활발한 임상적용이 이루어지고 있다. 자기공명영상법의 활발한 임상적 응용 및 새로운 technique이해를 위해서는 물리학적 개념(MR Physics)을 이해하는 것이 매우 중요하다.

• The Journal of the Korea Contents Association
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• v.5 no.4
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• pp.71-77
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• 2005

On contraction of the muscles, marked changes in X-ray reflections are observed, suggesting that conformational changes of contractile molecules and the movement of myosin heads during muscle contraction. It was found that the successive twitches decreased not only the time needed to the peak tension after the onset of stimulation but also the time needed to the maximum change of the X-ray intensity. However, the difference of the time between the peak tension and the maximum intensity change$(T_i-I_i)$ is nearly the same at any twitch.

• The Korean Journal of Nuclear Medicine
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• v.38 no.2
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• pp.140-142
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• 2004

As a molecular imaging is the most up-to-date technology in Nuclear Medicine, it has complicate ethical and regulatory problems. For animal experiment, we have to follow institutional animal care committee. for clinical experiment, we have to get approval of Institutional Review Board according to Helsinki declaration. In addition, approval from Korea Food and Drug Administration (KFDA) is essential for manufacturing and commercialization. However, too much regulation would suppress development of new technology, which would result in the loss of national competitive power. In addition, most new radioactive ligands for molecular imaging are administered to human at sub-pharmacological and sub-toxicological level. In conclusion, a balanced regulation is essential for the safety of clinical application and development of new technology.

• Nuclear Medicine and Molecular Imaging
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• v.43 no.3
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• pp.229-239
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• 2009

Molecular imaging strives to visualize processes in living subjects at the molecular level. Monitoring biochemical processes at this level will allow us to directly track biological processes and signaling events that lead to pathophysiological abnormalities, and help make personalized medicine a reality by allowing evaluation of therapeutic efficacies on an individual basis. Although most molecular imaging techniques emerged from the field of oncology, they have now gradually gained acceptance by the cardiovascular community. Hence, the availability of dedicated high-resolution small animal imaging systems and specific targeting imaging probes is now enhancing our understanding of cardiovascular diseases and expediting the development of newer therapies. Examples include imaging approaches to evaluate and track the progress of recent genetic and cellular therapies for treatment of myocardial ischemia. Other areas include in vivo monitoring of such key molecular processes as angiogenesis and apoptosis, Cardiovascular molecular imaging is already an important research tool in preclinical experiments. The challenge that lies ahead is to implement these techniques into the clinics so that they may help fulfill the promise of molecular therapies and personalized medicine, as well as to resolve disappointments and controversies surrounding the field.

• Journal of the Korean Chemical Society
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• v.63 no.5
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• pp.323-326
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• 2019