• The KIPS Transactions:PartB
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• v.15B no.6
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• pp.561-574
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• 2008

The spot detection phase of the 2-DE image analysis program segments a gel image into spot regions by an image segmentation algorithm and fits the spot regions to a spot shape model and quantifies the spot informations for the next phases. Currently the watershed algorithm is generally used as the segmentation algorithm and there are the Gaussian model and the diffusion model for the shape model. The diffusion model is closer to real spot shapes than the Gaussian model however spots have very various shapes and especially an asymmetric formation in x-coordinate and y-coordinate. The reason for asymmetric formation of spots is known that a protein could not be diffused completely because the 2-DE could not be processed under the ideal environment usually. Accordingly we propose an asymmetric diffusion model in this paper. The asymmetric diffusion model assumes that a protein spot is diffused from a disc at initial time of diffusing process, but is diffused asymmetrically for x-axis and y-axis respectively as time goes on. In experiments we processed spot matching for 19 gel images by using three models respectively and evaluated averages of SNR for comparing three models. As averages of SNR we got 14.22dB for the Gaussian model, 20.72dB for the diffusion model and 22.85dB for the asymmetric diffusion model. By experimental results we could confirm the asymmetric diffusion model is more efficient and more adequate for spot matching than the Gaussian model and the diffusion model.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.18 no.3
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• pp.687-696
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• 2014

This paper presents a semantic object extraction method using user's stroke input, color, and depth information. It is supposed that a semantically meaningful object is surrounded with a few strokes from a user, and has similar depths all over the object. In the proposed method, deciding the region of interest (ROI) is based on the stroke input, and the semantically meaningful object is extracted by using color and depth information. Specifically, the proposed method consists of two steps. The first step is over-segmentation inside the ROI using color and depth information. The second step is semantically meaningful object extraction where over-segmented regions are classified into the object region and the background region according to the depth of each region. In the over-segmentation step, we propose a new marker extraction method where there are two propositions, i.e. an adaptive thresholding scheme to maximize the number of the segmented regions and an adaptive weighting scheme for color and depth components in computation of the morphological gradients that is required in the marker extraction. In the semantically meaningful object extraction, we classify over-segmented regions into the object region and the background region in order of the boundary regions to the inner regions, the average depth of each region being compared to the average depth of all regions classified into the object region. In experimental results, we demonstrate that the proposed method yields reasonable object extraction results.