• Title/Summary/Keyword: 분리 및 선별

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The Development of Electrostatic Separation Technique for Recycling of Life Circles Waste Plastic (생활계 폐플라스틱 재활용을 위한 정전선별 기술개발)

  • Jeon Ho-Seok;Park Chul-Hyun;Kim Byoung-Gon;Park Jai-Koo
    • Resources Recycling
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    • v.15 no.1 s.69
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    • pp.28-36
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    • 2006
  • The development of material separation technique for waste plastic recycling are the necessary situation restricted by law the reclamation and incineration of waste plastic after 2004, with enforcement of EPR (Extended Producer Responsibility) system. As the this study is the research on the development of electrostatic separation techniques for recycling of life circles waste plastic by development of charging material and charger, the separation efficiency can be improved. Therefore, we developed the charger and electrostatic separator to increase charging efficiency and material separation per object plastics, rising these equipments, we removed PVC up to $99\%$ from two kinds of mixed plastics. And in case of restricting PVC content such as PET, we developed the separation technique that can remove PVC up to $99.99\%$ from PET with PET recovery about $80\%$. Also, as we separated over $98\%$ for three kinds of mixed plastics, and then established material separation technique to increase recycling of plastic.

Isolation of the Bacteria Strains with Highly Active β-glucosidase from Traditional Wine and Use them for the Synthesis of Non G lucoside Product in Plant (식물유래 비배당체 활성 성분 증대를 위한 고활성 β-glucosidase 생산 균주 선별 및 특성 규명)

  • Tae-Su Kim;Sua Im;Do Yun Jeong;Byung Hee Chun;Yun Ji Kim;Myoung Lae Cho;Bo-Ram Kim;Su Hui Seong;Jin-Woo Jeong
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2022.09a
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    • pp.125-125
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    • 2022
  • 식물 내에는 배당체 형태의 생리활성 물질들이 다량 존재하고 있으며, 비배당체로 전환하였을 경우 생리활성이나 투과성이 증대된다는 연구들이 보고되어 있다. 따라서 본 연구에서는 고활성 β-glucosidase 활성을 갖는 균주를 선별함으로써 식물 내의 비배당체 활성 성분을 증대시키는 데 활용하고자 한다. 균주는 전남지역 제조된 막걸리부터 분리하였다. 분리된 균주는 성장 속도 등 산업적으로 활용될 수 있는 가치를 평가하여 11종 22점의 균주를 분리하고, 16S rRNA분석을 통해 균주를 동정하였다. 동정된 균주를 이용하여 β-glucosidase의 활성을 평가하여 고활성 균주를 1종을 선별하였다. 선별된 균주는 다른 균주에 비하여 상대적 활성이 57% ~ 99%정도 활성이 높았으며, 공시 균주와 비교하였을 때도 상대 활성이 높은 았다. 선별된 균주의 특성을 규명하기 위하여 glycerol, L-arbinose를 비롯한 49가지의 탄수화물의 대사 활성을 확인해본 결과 25가지의 탄수화물 대사 활성을 가지고 있는 것을 알수 있었다. 또한 Lipase, β-glucosidase 19가지의 다양한 효소활성을 확인해본 결과 8종의 효소활성이 있음을 확인할 수 있었다.

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Development of Embedded Controller Based Color-Sorter System (임베디드 제어기 기반의 색채선별기 개발)

  • Kim, Ki-Sun;Son, Hung-Min;Kim, Young-Min;Tak, Chul-Gon;Park, Sang-Seog;Lim, Sang-Kyung;Ha, Jeong-Seok;Jeong, Min-Jeong;Lee, Yun-Jung
    • IEMEK Journal of Embedded Systems and Applications
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    • v.5 no.2
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    • pp.84-92
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    • 2010
  • 색채선별기는 양질의 곡물 유통을 위해 불량곡물, 이물질을 선별하는 시스템이다. 기존의 색채 선별기는 PC기반 제어시스템으로서 비교적 고가이고 부피가 크며 현장 환경에서 기계적 충격이나 먼지등에 취약하다는 문제점을 가지고 있다. 본 논문에서는 이러한 문제점을 개선하기 위해 운영체제가 탑재된 임베디드 제어기 기반의 색채선별기를 제안한다. 색채선별기는 기본적으로 곡물의 유입량 및 속도를 조절해 주는 피터부, 곡물의 영상을 받아 이미지를 처리해 주는 카메라부, 카메라부에서 받은 정보를 이용해 양품과 불량품을 분리해 주는 이젝터부로 구성된다. 본 논문에서는 개발된 지능형 색채선별기용 ARM 프로세서 기반 임베디드 제어기의 하드웨어 구성, 피더부, 카메라부, 이젝터부 간의 통신프로토콜을 이용한 총괄관리 제어와 그래픽 사용자 인터페이스(GUI)를 소개한다.

Removal and Separation of Metallic Constituents from the By-product Recovered from Gold Mine Tailings (금(金) 광산(鑛山) 폐광미(廢鑛尾)로부터 회수(回收)된 금속광물(金屬鑛物) 부산물(副産物) 중의 금속성분(金屬成分) 분리(分離), 제거연구(除去硏究))

  • Youn, Ki-Byoung
    • Resources Recycling
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    • v.19 no.3
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    • pp.9-15
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    • 2010
  • Domestic gold mine tailings, generally, contain a lot of non-metallic silica and clay minerals. These minerals can be separated from the tailings by various physical separation methods and used as raw materials for cements and ceramic products. In these physical separation procedures, metallic complex sulfides, in which Au and metallic constituents such as Pb, As and Fe were concentrated, were obtained as a by-product. These metallic constituents should be removed or separated from the by-product to extract Au efficiently. In this work, removal and separation processes of Pb, As, and Fe from the by-product were investigated. Pb was removed to under 3% by using alkaline oxidative leaching at the leaching condition of $120^{\circ}C$, 2M NaOH, 100psi $Po_2$, 250r.p.m., 4 wt.% solid and 30 min. leaching time. The leached residue was roasted and separated magnetically to obtain a non-magnetic product contained <0.2% As, <3% Fe and high concentrated Au more than 8,000 ppm.

신호 전달 체계에 있어서의 Phospholipase C에 대한 연구

  • 민도식;김재호;이영한;서판길;류성호
    • Proceedings of the Korean Society of Applied Pharmacology
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    • 1992.05a
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    • pp.27-27
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    • 1992
  • 많은 홀몬, 성장인자 및 신경 전달물질들은 각각에 대한 세포막의 수용체와 결합하여 Phospholipase C (PLC)를 활성화시키므로서 신호를 세포내로 전달하여 세포의 성장, 대사, 신경 흥분, 수축 및 분비 등의 생리 현상을 나타내고 있다. 이 신호 전달의 중심 효소인 PLC는 현재까지의 효소 분리, 유전자 클로닝 등의 방법으로 3가지 Class에 적어도 8종유의 등위효소(Isozyme)들이 존재하고 있는 것으로 밝혀지고 있다. 본 연구에서는 이와 같은 등위효소에 특이적인 조절 물질을 선별할 수 있는 체계를 확립하기 위하여 새로운 동위효소의 분리 및 규명과 이 동위효소들에 대한 과발현 및 발현 세포주 개발을 추진하고 있다.

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Fibrinolytic, Immunostimulating, and Cytotoxic Activities of Microbial Strains Isolated from Kochujang (고추장 분리 균주의 혈전용해능, 면역활성능 및 세포독성 효과 조사)

  • Seo, Mi-Young;Kim, Seung-Ho;Lee, Cheol-Ho;Cha, Seong-Kwan
    • Korean Journal of Food Science and Technology
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    • v.39 no.3
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    • pp.315-322
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    • 2007
  • This study was carried out to investigate the functional activities of microorganisms isolated from kochujang, such as fibrinolytic, immunostimulating, and cytotoxical activities, and to apply these microorganisms to kochujang products. Ninety-one microbial strains with proteolytic activity were selected from 294 strains isolated from traditional and commercial kochujang. Three strains (TPP 0014, TPP 6013, and TPP 6015) with high fibrinolytic activity were tested for their immunostimulating and cytotoxical activities. For the assessment of macrophage activation, cytokines such as tumor necrosis factor, $interleukin-1{\alpha}$ and nitrogen oxide were measured with the murine macrophage cell line RAW 264.7. In addition, the cytotoxical activities of the three strains were examined by MTT assay on the colon cancer cell line SNU-C4 and normal cell line CHO-K1. Using an API identifying kit, two of the microbial strains (TPP 0014 and TPP 6015) were identified as Bacillus stearothermophilus and the other strain (TPP 6013) was identified as B. amyloliquefacience.

The Development of Material Separation Technique for Recycling of Waste Car Tail Lamp (폐자동차(廢自動車) Tail Lamp의 재활용(再活用)을 위한 재질분리(材質分離) 기술개발(技術開發))

  • Jeon, Ho-Seok;Park, Chul-Hyun;Baek, Sang-Ho;Park, Jai-Koo
    • Resources Recycling
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    • v.15 no.6 s.74
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    • pp.25-32
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    • 2006
  • In this study, waste car tau lamps that usually gets incinerated or buried has been recovered during the dispose process in order to increase the recycling rate of waste cars and the material separation research was performed by applying triboelectrostatic separation method. After a series of charging characteristic test, PMMA materials was confirmed of being an effective charging material for waste car tail lamp material separation, a cyclone charger using PMMA material for continuous process was developed. In optimum test conditions using triboelectrostatic separator developed in this study, high quality PMMA material with recovery rate of 99.0% and 90.2% was achieved from the waste car tail lamp. Therefore, a material separation technology that can recycle tail lamps of waste cars has been established.

Gravity Separation Characteristic for the Gold.Silver Ores on the Philippine Mankayan District (필리핀 만카얀 지역 금.은 광석의 비중선별 특성)

  • Kim, Hyung-Seok;Chae, Soo-Chun;Kim, Jeong-Yun;Sohn, Jeong-Soo;Kim, Sang-Bae
    • Journal of the Mineralogical Society of Korea
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    • v.21 no.4
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    • pp.383-395
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    • 2008
  • To enhance the grade and recovery rate of the gold/silver ores which yield at Philippine Mankayan mine, we studied the characteristics which are the geologic and mineralogical features of gold and silver ore, the liberation by crushing and grinding, the separation by sieving and shaking table. Gold/silver ore is composed of the sulfide minerals like pyrite, sphalerite, galena; and the gangue minerals which is quartz, clay. Gold/silver element are mainly contained in a sulfide minerals like pyrite, sphalerite and galena. To increase the liberation rate of sulfide minerals containing gold/silver element, the gold/silver ore has to be grounded under $100{\mu}m$ very finely because the crystal size of sulfide minerals is distributed from $1{\mu}m$ to $100{\mu}m$. The liberation rate of gold/silver ore increases to 92% when the particle size ($d_{90}$) of ore is grounded below $100{\mu}m$ by jaw crusher $\to$ cone crusher $\to$ rod mill by steps. The grade and recovery of sulfide minerals could not be enhanced by sieving separation because those crystal size is distributed homogeneously below $100{\mu}m$. But, when we separated the sieved ore using shaking table, the gold and silver grade increased to 40 ppm and 140 ppm, respectively. Then the recovery rate of gold reach almost 100% but that of silver is no more that 50%.

Identification of Fluorescent Pseudomonads Producing Siderophore and Construction of Siderophore Biosynthesis Defective Mutant (Siderophore를 생성하는 Fluorescent Pseudomonads의 분리, 동정 및 돌연번이 유기)

  • Park, Yeal;Kim, Hyun Hee;Myeong-gu Yeo;Young-woo Seo;Han-cheol Koh;Young-gi Yang;Hyeon-Sook Cheong;Sung-jun Kim
    • Korean Journal of Microbiology
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    • v.30 no.4
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    • pp.286-290
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    • 1992
  • The present study was performed to isolate the fluorescent pseudomonads from Kwang-Ju soil and to construct a mutant strain defective in siderophore biosynthesis. The siderophore-secreting pseudomonads were screened on Blue agar (Chrome Azuol S agar) plates and one strain of them was designated to Pseudominas fluorescens (P. fluorescens) PY002. To construct a mutant defective in siderophore biosynthesis, P. fluorescens PY002 was randomly mutagenized with a transposon Tn5. The location of Tn5 integrated into chromosomal of the mutants strain was determined by Southern blot analysis. The mutagenized strain showed non-fluorescent on a King's B agar plate and were defective in iron (III) acquisition ability.

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Selective Media for Isolation and Enumeration of Lactic Acid Bacteria from Kimchi (김치에서 젖산균의 선발 및 계수를 위한 선택배지)

  • 이명기;박완수;강국희
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.25 no.5
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    • pp.754-760
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    • 1996
  • Selective media for isolation and enumeration of lactic acid bacteria from kimchi were compared using 36 strains of lactic acid bacteria from kimchi and dairy products. Among the selective media, KF Streptococcus agar showed the best result for the selection of Enterococcus group and Pediococus group, and M-17 agar with pH indicator for Lactococcus group, the m-LBS agar for Lactobacillus group, and PES-3 agar for Leuconostoc group.

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