• Applied Biological Chemistry
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• v.47 no.2
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• pp.192-196
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• 2004

In order to measure cellular physiological activity including ion channel activity, protoplasts were isolated from the root tissue of tomato plant. The general methods recommended were not efficient enough to make protoplasts from the root tissue. Among various conditions tested, we found that a two-step treatment of osmosis is very efficient for the isolation of protoplasts. In this procedure, root tissues were preincubated in a solution containing 300 mM sorbitol for 30 min. Then, they moved to the reaction solution containing 700 mM sorbitol as well as cell wall-digesting enzymes. The formation of protoplast was greatly increased by this method. In order to find the optimal condition of the two-step method, various conditions of pH, osmotic pressure, incubation time, and the concentrations of cell wall-digesting enzymes were tested. The yield of protoplast isolation was maximal at pH 5.0 after 2 hr incubation. Mixed enzymes of 3% cellulase, 1 % macerozyme, and 0.1 % pectolyase showed maximal protoplast isolation. The physiological activity of isolated protoplast evaluated by measuring the cellular ATPase activity was as high as that measured from the preparation of root tissue. The protoplasts isolated by this method were remained healthy up to 4 hrs which is enough time to measure the cellular physiological activity. These results show that the two-step treatment of osmotic pressure was successful to obtain high yield of healthy protoplast from tomato root tissue.

• Journal of the Korean Society for Aeronautical & Space Sciences
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• v.38 no.10
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• pp.1031-1037
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• 2010

This paper proposed lightweight aluminum bipolar plates as an alternative for conventional graphite bipolar plates in fuel cell systems used as a power source for small reconnaissance UAVs. Since bipolar plates occupy more than 80% of the total weight of the fuel cell system, lightweight aluminum bipolar plates can improve the overall payload and flight time of the fuel cell UAV. The aluminum and graphite bipolar plates were fabricated to compare the performance of each of them. A 15% higher performance per weight was obtained from aluminum bipolar plates than the graphite bipolar plates. Also, the performance of a single cell using aluminum bipolar plates was evaluated under various operating conditions.

• Current Industrial and Technological Trends in Aerospace
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• v.7 no.1
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• pp.97-104
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• 2009

Successful separation of satellites from launch vehicles and release its appendages such as solar arrays and antennas are one of the most important tasks for mission accomplishment during in-orbit maneuver. Especially, specific release devices called NEA(Non-Explosive Actuator) have been widely adopted to perform safe separation and release due to its outstanding performance of low functional shock (below 500g), no contamination and easy handing as opposed to the pyroshock device. In the paper, various kinds of NEA and its history of development are reviewed along with a summary on the domestic research trend.

• Proceedings of the Korean Vacuum Society Conference
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• 2011.08a
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• pp.344-344
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• 2011

진공인터럽터는 진공차단기에서 실제 사고전류차단시 발생하는 아크소호 및 차단을 담당하는 매우 중요한 부분이다. 일반적으로 교류차단기는 일시적으로 에너지가 공급되지 않는 전류 영점에서 아크소호 및 전류차단이 이루어진다. 일반적으로 아크가 발생하는 전극분리시점에서 아크가 소호되는 전류영점까지의 시간을 아크지속시간(arcing time)이라고 한다. 일단 진공 인터럽터 내부의 접점이 분리됨과 동시에 아크가 발생하게되면 이때 진공인터럽터는 아크지속시간동안 고온의 아크에 의한 접점손상을 최소화하기 위해 아크의 거동을 적절히 제어하여 아크에너지를 접점표면상에 골고루 분산시켜야 한다. 현재 진공인터럽터에 사용되는 아크제어 방식에는 크게 횡자계 방식과 축자계 방식이 있다. 본 논문에서는 횡자계 방식 중 spiral type 접점을 사용하여 25 kA의 전류차단시험을 진행하면서 아크전압을 취득하는 동시에 아크이미지를 촬영하여 아크지속시간동안의 아크의 거동을 분석하였는데 특히 본 실험에서는 전체 아크지속시간동안의 아크상태천이과정이 가장 잘 관찰되는 비교적 긴 아크지속시간을 설정하여 실험을 진행하였으며 이때 접점손상이 아크거동 및 차단성능에 미치는 영향을 중점적으로 다루었다.

• Proceedings of the Korean Vacuum Society Conference
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• 2014.02a
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• pp.398.2-398.2
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• 2014

유기태양전지는 낮은 공정 단가, 저온공정 및 기계적 유연성과 같은 다양한 장점을 지니고 있어서 실리콘 기반의 태양전지를 대체하기 위해서 많은 연구가 진행되고 있다. 유기태양전지는 실리콘 기반의 태양전지에 비해서 낮은 광전변환효율을가지고 있기 때문에 효율을 높이기 위한 다양한 연구가 진행되고 있다. 그 중에서 엑시톤 분리 효율을 높이기 위한 방안으로 나노 구조가 많이 연구되고 있다. 하지만 나노 구조를 제작하기 위해서는 식각 과정을 거치거나, 금속 템플레이트를 사용하여 공정상 복잡하고 어려움을 갖는다. 본 연구에서는 간단한 용액 공정을 이용하여 초음파 처리시간 변화에 따른 나노 구조를 가지는 광활성층을 제작하였다. 전자주게 물질인 P3HT를 혼합 용매에 녹여서 초음파 처리를 통해서 나노 구조를 제작하였고, 초음파 처리 시간에 따른 구조의 변화를 광류미네에센스 측정과 원자간 힘 현미경으로 관찰하였다. 나노 구조를 가지는 태양 전지는 엑시톤을 분리할 수 있는 전자주게와 전자받게의 계면이 증가함으로 엑시톤 분리 효율이 향상되는 장점을 가진다. 초음파 처리 사긴 변화에 따른 나노 구조 P3HT 층을 가진 태양전지의 전류밀도-전압 측정을 통해 효율의 변화를 비교하였다. 15분 동안 초음파 처리를 하였을 때, 가장 높은 효율을 가지는 것을 확인할 수 있었고, 나노 구조를 가지지 않는 유기태양전지에 비해서 20% 정도 효율이 향상되는 결과를 볼 수 있었다.

• Korean Journal of Food Science and Technology
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• v.31 no.3
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• pp.864-869
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• 1999

Synergistic effects of the falcarindiol mixed with monoglyceride in broth exhibited strong growth inhibition on all test strains. It was confirmed that the falcarindiol mixed with monoglyceride exhibited bactericidal effect in broth against L. monocytogenes ATCC 19111 and ATCC 19114 at the same level.

• Korean Journal of Veterinary Research
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• v.24 no.2
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• pp.137-147
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• 1984

Cells with an epithelioid morphology were isolated from the rabbit myometrium and were grown in culture. The cells had a doubling time of 53 hours when grown in the presence of 10% fetal calf serum in Basal Eagle's medium with 3mM glutamine. In the presence of estrogen plus insulin, doubling time was reduced to 40 hours. Creatine kinase activity upon reaching confluency was determined to be 0.019 unit per mg protein. Approximately 30% of the activity was extractable only in high ionic strength buffer. Cells also contained plasminogen activator with a specific activity of 140 CTA units per million cells. Creatine kinase was mainly BB form. The cells contained a cross reactive protein against bovine smooth muscle uterine anti-myosin.

• KSBB Journal
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• v.21 no.3
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• pp.220-223
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• 2006

Lysozyme is an important antibacterial material, as effective food preservative. A number of lysozymes are found in nature such as egg white, where exists about 3.5% of egg proteins. In this study, carboxymethyl cation exchange chromatography has been used for separation of lysozyme. A simulation study by ASPEN was also performed for saving time and cost in chromatography purification experiments. Important parameters in experimental chromatography were sample loading amount, NaCl concentration, and pH of eluent. Simulation results were successfully fitted with chromatograms from experiments with change of parameters mentioned above.

• Korean Chemical Engineering Research
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• v.47 no.5
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• pp.615-619
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• 2009

(+)-$Tr{\ddot{o}}ger$'s base in $Tr{\ddot{o}}ger$'s base racemates that inhibits thromboxane A2($T{\times}A2$) synthase has been used to treat arteriosclerosis. Separation of (+)-$Tr{\ddot{o}}ger$'s base by chromatography has become a major concern. However separation experiments of (+)-$Tr{\ddot{o}}ger$'s base need time and consumables so that simulation with Aspen Chromatography could save time and costs by predicting the efficiency of separation. Injection amount and eluent flow rate were varied to compare the resolutions and yields of TB(-) and TB(+). Highest resolution and yield were attained at the eluent rate of 0.25 mL/min. Isotherms representing the relationship between mobile phase concentration and stationary phase concentration were changed to get the best separation with Ideal Adsorbed Solution(IAS) Statistical Lanmuir isotherms.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.34 no.5C
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• pp.505-513
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• 2009

This paper proposes a real-time foreground segmentation and background substitution method for protecting the privacy on visual communication. Previous works on this topic have some problems with the color and shape of foreground and the capture device such as stereo camera. we provide a solution which can segment the foreground in real-time using fixed mono camera. For improving the performance of a foreground extraction, we propose the Temporal Foreground Probability Model (TFPM) by modeling temporal information of a video. Also we provide an boundary processing method for natural and smooth synthesizing that using alpha matte and simple post-processing method.