• Title/Summary/Keyword: 분리기구

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Chorion Gene Expression in the Cellular Differentiation and Accumulation of Chorion Protein Synthesis of Silkmoth, Bombyx mandarina(Lepidoptera: Bombycidae). II. Synthesis Programme of Chorion Proteins (한국산 멧누에나방(Bombyx mandarina)에 있어서 난각유전자의 형질발현. II. 난각단백질의 합성과정)

  • 노시갑
    • Korean journal of applied entomology
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    • v.32 no.4
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    • pp.420-425
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    • 1993
  • The programme of silkrnoth chorion protem synthetIc changes is autonomous i. e.,it is imple I mented WIth normal kinetics by follicles cultured in isolation in a defined tIssue culture medium. On the basis of protem synthetic profiles, 8 stages of chorwgenesis are defined. Detail a analysis of the proteins of chorion of Bombyx mandarina, reveals more than 17 components by eletrophoretic mobility and synthetic kinetics. We have defined three abbreviated synthetlc s stages, based on the pattern of protein synthesized at early, middle, and late synthetic stages

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Electrochemical Study on the 3-Phenyl-4-Nitrosydnone (3-Phenyl-4-Nitrosydnone의 전기화학적 연구)

  • Il-Kwang Kim;Youn-Geun Kim;Soon-Jong Han
    • Journal of the Korean Chemical Society
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    • v.32 no.3
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    • pp.195-202
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    • 1988
  • An electrochemical reduction on the 3-phenyl-4-nitrosydnone in acetonitrile solution has been studied by direct current, differential pulse polarography, cyclic voltammetry and controlled potential coulometry. Before the cleavage of phenyl-N single bond a irreversible electron transfer-chemical reaction(EC) mechanism of nitro functional group proceeded to form amino (or-hydroxylamino) group by multielectron transfer which is followed to give phenyl hydrazine by single electron transfer-chemical reaction at the 2nd and 3rd irreversible reduction wave of high negative potential region. The cathodic half-wave potentials shown to be shift negative due to inhibitory effect of cetyl-trimethyl ammonium bromide micelle while reversible anodic peaks on the 2nd and 3rd reduction waves in the presence of NaLS at high negative potential region.

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The Particle-Initiated Breakdown Characteristics on the Spacer Surface for $SF_6$GIS ($SF_6$GIS용 스페이서 표면 파티클에 의한 절연파괴 특성)

  • 김정달;이세훈
    • Journal of the Korean Institute of Illuminating and Electrical Installation Engineers
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    • v.12 no.1
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    • pp.3-11
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    • 1998
  • The influence due to particle contaminated on spacer surface is remarkable in the decreasing of dielectric strength in SF6 GIS. In relation with this problem, We studied, AC flash-over voltage characteristics and breakdown mechanism are investigated under metallic and insulating particle initiated condition in SF6 gas by varying the particle position, particle shape with a plane-plane electrode. The main results are as follows $\circled1$ Influence of the flash-over voltage decrease for particle position is lowest in mid gap and is highest in separated small gap on electrode. $\circled2$ The metallic particle shape which results in the more reduced flash-over voltage, the FOV saturation moved in to the region of low pressure. $\circled3$Insulating particle less than 0.6[mm] are not almost influenced by the flash-over Voltage.oltage.

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The Operation Concept and Procedure of Mechanical Ground Support Equipment for KSLV-II Launch Complex (한국형발사체 발사대시스템 지상기계설비 운용개념 및 절차)

  • Lim, Chankyoung;Kim, Daerae;Yang, Seongpil;Lee, Yeongho;Kang, Sunil
    • Journal of the Korean Society of Propulsion Engineers
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    • v.22 no.4
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    • pp.125-132
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    • 2018
  • The mechanical ground support equipment of a Korea Space Launch Vehicle-II launch complex is a collection of systems used for transporting, erecting and lowering the launch vehicle. It also provides an interface for supplying propellants. In this paper, compositions, functions and design results of mechanical ground support equipment are introduced. Additionally, the operational concept of each piece of equipment is presented with operation procedure.

Isolation and Characterization of Microorganisms with Broad Antifungal Activity against Phytopathogenic Fungi (식물병원균에 광범위 항균활성을 가진 미생물의 분리 및 특성)

  • Kim, Min-Hee;Ko, Hee-Sun;Yook, Young-Min;Kim, Hyun-Soo
    • KSBB Journal
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    • v.23 no.3
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    • pp.219-225
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    • 2008
  • For the production of an antifungal compound, one strain (I-8) was selected from approximately 400 strains isolated from various soil samples. The optimum carbon source, nitrogen source and pH culture conditions for the production of the antifungal compound were investigated. ISP No. 2 medium (yeast extract 0.4%, malt extract 1% and dextrose 0.4%, at pH 8) was determined to be the optimum medium. Strain I-8 showed broad antifungal activity against the plant pathogenic fungi tested, including Sclerotinia sclerotiorum KACC 41065, as well as cellulase and chitinase activities in an agar plate assay. The extraction of antifungal compounds was performed using ethyl ether and ethyl acetate. In a culture broth of strain I-8, the ethyl acetate extract exhibited effective growth inhibition against 14 of the 20 phytopathogenic fungi tested. By mixing the ethyl acetate extract from I-8 with the ethyl ether extract from the fungus 13-16, which shows specific antifungal activity against Colletotrichum orbiculare KACC 40808, the antifungal activity of I-8 against phytopathogenic fungi was confirmed to be slightly increased. Strain I-8 showed strong growth inhibition against 16 phytopathogenic strains in agar plate tests.

Isolation and Characterization of xylR/TMutants in Escherichia coli (대장균(大腸菌)의 xylRjT 변이주(變異株)의 분리(分離) 및 그 특성(特性))

  • Roh, Dong Hyun;Rhee, In Koo
    • Current Research on Agriculture and Life Sciences
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    • v.10
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    • pp.125-135
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    • 1992
  • Nine strains of xyl mutants that could not utilize xylose as a carbon source were isolated from E. coli JM109 by the treatment of NTG in order to investigate the regulation of xylose operon and to use recipient cells for the cloning of xylR gene. For the characterization of all isolated mutants, colony colors of all mutants on MacConkey-xylose and MacConkey-xylulose agar plate were observed for the utilization of xylose and xylulose, and the growth level and the activity of xylose isomerase and xylulokinase were determined in need. The isolated xylR/T mutants formed the white colony on MacConkey-xy-lose and MacConkey-xylulose agar plate. They did not detect the activity of xylose isomerase, and the activity of xylose isomerase was not restored in transformants of xylR/T mutant with pEX13 which contained xylA gene. xylR and xylT mutants were classified from xylR/T mutants depending upon the growth level in minimal medium. xylT mutants; DH13, DH121 and DH125 could grow a little in that medium, but xylR mutants; DH10, DH53, and DH60 could not grow that medium.

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Studies on the $\beta$-Galactosidase from Thermphilic Bacterium - Physiological Characteristics of the Selected Thermophile - (고온성 세균의 $\beta$-Galactosidase에 관한 연구 ( I ) - 분리고온균의 생리적 특성 -)

  • 이종수;오만진;이석건;김찬조
    • Microbiology and Biotechnology Letters
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    • v.11 no.1
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    • pp.5-13
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    • 1983
  • This experiment was carried out to elucidate the thermotolerant properties of a thermophilic bacterium which isolated from soils of the hot springs area and selected for the $\beta$-galactosidase production. Biochemical and physiological characteristics of this strain were studied, including the investigation on the fatty acid composition for its neutral fats. The results obtained were summarized as follows. 1. This bacterium was identified as a strain belong to the genus Thermus. 2. Optimal temperature and pH for growth of this strain were $65^{\circ}C$ and pH 6.5 respectively, and it was found to be an absolute thermophilic bacterium which could not grow at 37$^{\circ}C$. 3. No growth was obtained in the medium which contained more than 1.0% of sodium chloride. 4. The tolerable concentration of antobiotics were 10$\mu\textrm{g}$ of penicillin G per $m\ell$ of medium and 0.5$\mu\textrm{g}$ of chloramphenicol per $m\ell$ respectively 5. This strain had autotrophilic requirements for calcium-pantothenate and pyridoxine-HCO as an-essential factor and for niacin as a stimulative factor. 6. Fatty acid composition of neutral fats of the strain was palmitic acid. 60.20%; lauric acid, 11.8%; myristic acid, 7.56%, behenic acid, 4.25%; capric acid, 1.77%; stearic acid, 2.13%; arachidic acid, 1.53%; and others unidentified, 10.7%.

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Analytical study to the Brake Lever in Basic Brake System for Railway Vehicle (철도차량용 기초제동장치의 제동레버 강도에 대한 해석적 연구)

  • Park, Su-Myung;Park, Jae-young
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.17 no.8
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    • pp.624-629
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    • 2016
  • A brake lever in a basic railway brake system is an important safety device that delivers braking force from the brake cylinder to the brake pad. The safety guidelines for designing rolling stock only qualitatively describe that the brake lever should have sufficient strength. Each train has a different type of brake lever. One brake lever that was designed with a factor of safety of 1.27 has failed, so the material was changed to increase the strength. Therefore, the stress distribution and weak points of the lever were identified by theoretical analysis. and structural analysis. Different brake lever designs were examined for KTX high-speed trains, which have a split-type structure, as well as for electric locomotives, which use an electric multiple unit (EMU) with a unity-type structure. A fracture test was also done to look at the relationship between the vertical stress and the bending stress during braking. The results were used to find a safety factor to apply to each train and suggest quantitative minimum guidelines. We also looked at changing the unity-type EMU brake lever to the split type under the same conditions and analyzed how much the design change affected the factor of safety.

Extraction of Yttrium and Europium with Fatty Acids as Extractants (지방산계 추출제에 의한 이트륨과 유로피움의 분리)

  • Bang, Kyung-Mo;Ryu, Ho-Jin;Choi, Min-Bae;Kang, Ho-Cheol
    • Applied Chemistry for Engineering
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    • v.21 no.5
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    • pp.509-513
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    • 2010
  • Recently, an amount of waste electronic devices such as LED and trichromatic fluorescent lamp has increased with the development of electronic industry. Reportedly, rare earth metals such as yttrium and europium have been discovered in the waste electronic devices. In order to improve the selectivity of yttrium and europium, the effects of the following factors on recovery experiment have been considered : i) fatty acids with various alkyl chain lengths, ii) the concentration of extractant, and iii) pH. The results show that the extraction efficiencies decrease at the same pH with decreasing the concentration of extractant and so $pH_{0.5}$ (That value of pH in an aqueous phase at which the distribution ratio is unity at equilibrium: 50% of the solute is extracted (E = 0.5) only when the phase ratio is unity.) moves into higher pH. The highest selectivity of yttrium and europium was obtained with tetradecanoic acid as extractant. The extraction mechanism of yttrium and europium was varied with the change of concentration of the tetradecanoic acid. $MR_3$single-species was formed from the yttrium and europium ion in the extractant concentration less than 0.1 M. On the other hand, the yttrium or europium ion is solvated with three molecules of tetradecanoic acid monomer like $MR_3{\cdot}$ 3RH in the extractant concentration more than 0.1 M.

Template-free Hydrothermal Synthesis of High Phase Purity Mordenite Zeolite Particles Using Natural Zeolite Seed for Zeolite Membrane Preparation (제올라이트 분리막 제조를 위한 유기주형 없는 고순도 모데나이트 제올라이트 입자 수열합성에 관한 연구)

  • Lee, Du-Hyoung;Alam, Syed Fakhar;Lee, Hye-Rheon;Sharma, Pankaj;Cho, Churl-Hee;Han, Moon-Hee
    • Membrane Journal
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    • v.26 no.5
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    • pp.381-390
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    • 2016
  • In this study, the natural mordenite (MOR) zeolite seeds were used for the synthesis of high purity mordenite crystals. The effect of seed concentration and crystallization time on the phase purity and surface morphology of MOR crystals has also been reported. The diffraction, elemental and scanning analysis of MOR zeolite particles obtained from 100 g hydrothermal solution batch containing 3 g natural seed, hydrothermally treated at $140^{\circ}C$ for 72 h reveal the high phase-purity of as-synthesized sample having crystals of uniform size ($1-2{\mu}m$). Moreover, high seed concentration leads to the production of mesoporous MOR particles composed of needle shape primary nano crystallites. The gases adsorption performances of as-synthesized MOR particle were carried out at $25^{\circ}C$ and 0-1 bar. Surprisingly, MOR particles show good adsorption potential for $CO_2$ (97.19 mg/g) compared to other gases. Thus it confirms that high purity MOR particles can be synthesized without using any organic template which gives an advantage of separation performance at lower price.