• Title/Summary/Keyword: 복합처리

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Effects of Cycloheximide on Development of In Vitro Matured Porcine Oocytes Activated following Eelectric Pulse (전기자극 후 Cycloheximide 처리가 돼지난자의 활성화에 미치는 효과)

  • 송상현;정기화;조헌조;박충생
    • Korean Journal of Animal Reproduction
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    • v.26 no.1
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    • pp.79-85
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    • 2002
  • We investigated the optimal concentration and exposure time of cycloheximide(CHX) on development of activated porcine oocytes following electrical pulse(EP). After 42~44 h maturation, oocytes were treated with 0.1% hyaluronidase, and denuded cumulus cells by pipetting. Oocytes were stimulated by electric pulse (1.2 kV/cm, 30 $\mu$sec, 1 pulse) or incubated for 3, 5 and 7 h in cycloheximide (1, 5 and 10 $\mu\textrm{g}$/$m\ell$, respectively) following electric pulse, and cultured for 8 days. Cleavage rate of oocytes activated with 10 $\mu\textrm{g}$/$m\ell$ CHX following EP was significantly (P<0.05) higher than those of 1 $\mu\textrm{g}$/$m\ell$ (86.8% vs. 74.4%). The developmental competence of oocytes incubated to 5 $\mu\textrm{g}$/$m\ell$ of CHX was significantly (P<0.05) higher development to blastocysts (13.3%), compared with 10 $\mu\textrm{g}$/$m\ell$ of CHX (5.6%). When the oocytes were activated with 5$\mu\textrm{g}$/$m\ell$ CHX for 3, 5, and 7 h following EP, the cleavage rate of oocytes in 5 h group(86.6%) was significantly (P<0.05) higher than that in 3 h group(73.2%). The developmental rate of oocytes to morula in 5 and 7 h groups(26.7% and 16.4%) were significantly (P<0.05) high than that in 3 h group(14.5%). Matured oocytes were activated with electric pulse (EP) or electric pulse combined with cycloheximide (EP + CHX) and cultured for 8 days. The rate of cleavage and development to blastocyst (80.1% and 11.6%) of activated with EP group were similar to EP combined with CHX group. When activated with EP or EP combined with CHX, the mean cell number of blastocysts were less in the activated with EP (18.67$\pm$5.53) than in the activated EP combined CHX (20.71$\pm$6.16), but not significantly different. This results suggest that, when the porcine oocytes were activated with CHX following EP, the developmental rate of activated oocytes can be improved by treated with a concentration of 5 $\mu\textrm{g}$/$m\ell$ CHX for 5 h exposure time.

Characteristics of Silane Treated Graphene Filled Nanocomposites Exposed to Low Earth Orbit Space Environment (저궤도 우주환경하의 실란처리된 그래핀 첨가 나노 복합재료의 물성특성)

  • Noh, Jae-Young;Jin, Seung-Bo;Kim, Chun-Gon
    • Composites Research
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    • v.28 no.3
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    • pp.130-135
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    • 2015
  • This study investigates the property of graphene filled polymer nanocomposites in LEO(Low Earth orbit) environment conditions. In order to improve compatibility with polymer matrices and resistance of carbon material against AO(Atomic oxygen) attack, silanization of graphene oxide with organosilane was carried out. The corresponding moieties were characterized through X-ray photoelectron spectroscopy (XPS). Graphene oxide filled nanocomposites were prepared using solution based processing methods. The sets of specimen series were tested in an accelerated LEO simulated space environment facility. Graphene oxide and silane treated graphene oxide reinforced nanocomposites were compared with neat epoxy. The comparison revealed that the silane treated graphene filled polymer composite shows inherent resistance against atomic oxygen attack while the lack of silane treatment resulted in a reduction in performance.

Effect of Ozone Treatment of Carbon Nanotube on PTC/NTC Behaviors of High-Density Polyethylene Matrix Composites (오존처리에 따른 탄소나노튜브 강화 고밀도 폴리에틸렌 기지 복합재료의 PTC/NTC 특성)

  • Park, Soo-Jin;Seok, Su-Ja;Lee, Jae-Rock;Hong, Sung-Kwon
    • Polymer(Korea)
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    • v.29 no.1
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    • pp.32-35
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    • 2005
  • In this paper, the carbon nanotubes (CNTs) were ozonized and the positive temperature coefficient (PTC) behaviors of CNTs-filled high-density polyethylene (HDPE) conductive composites were studied. The results of element analysis (EA) and FT-IR indicate that the oxygen-containing functional groups on the CNTs surfaces, such as O-H, C-O, and C=O groups, were increased with the ozonization. Electrical resistivities of the CNTs/HDPE composites were measured by using a digital multimeter. The resistivity of the composites was increased abruptly near the crystalline melting temperature of the HDPE used as matrix, which could be attributed to the destruction of conductive network by the thermal expansion of HDPE. And, the PTC intensity of the CNTs/HDPE composites was increased with the increase of the ozone treatment time. It was probably due to the growing of maximum volume resistivity of the composites induced by the increased oxygen-containing functional groups in the CNTs surfaces.

Comparison of Resin Impregnation and Mechanical Properties of Composites Based on Fiber Plasma Treatment (섬유 플라즈마 처리에 따른 복합재료의 수지 함침성 및 기계적 특성 비교)

  • Seong Baek Yang;Donghyeon Lee;Yongseok Lee;Dong-Jun Kwon
    • Composites Research
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    • v.36 no.6
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    • pp.388-394
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    • 2023
  • In composites manufacturing, increasing resin impregnation is a key way to speed up the manufacturing process and improve product quality. While resin improvement is important, simple fiber surface treatments can also improve resin flowability. In this study, different plasma treatment times were applied to carbon fiber fabrics to improve the impregnation between resin and fiber. Electrical resistivity measurements were used to evaluate the dispersion of resin in the fibers, which changed with plasma treatment. The effect of fiber surface treatment on resin spreadability could be observed in real time. When inserting a carbon fiber tow into the resin, the amount of resin that soaked into the tow was measured to objectively compare resin impregnation. Five minutes of plasma treatment improved the tensile and compressive strength of the composite by more than 50%, while reducing the void content and increasing the fire point impregnation flow rate. Finally, a dynamic flexural fatigue test was conducted using a portion of the composite used as an architectural composite part, and the composite part did not fail after one million cycles of a 3 kN load.

Nondestructive Microfailure and Interfacial Evaluation of Plasma-Treated PBO and Kevlar Fibers/Epoxy Composites using Micromechanical Test and Acoustic Emission (Micromechanical 시험법과 음향방출을 이용한 플라즈마 처리된 PBO와 Kevlar 섬유강화 Epoxy 복합재료의 비파괴적 파단특성 및 계면물성 평가)

  • 박종만;김대식;김성룡
    • Composites Research
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    • v.16 no.4
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    • pp.74-79
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    • 2003
  • Comparison of interfacial properties and microfailure mechanisms of oxygen-plasma treated poly(p-phenylene-2,6-benzobisoxazole(PBO. Zylon) and poly(p-phenylene terephthalamide)(PPTA, Kevlar) fibers/ epoxy composites were investigated using micromechanical technique and nondestructive acoustic emission(AE). Interfacial shear strength(IFSS) and work of adhesion, Wa of PBO or Kevlar fibers/epoxy composites increased by oxygen-plasma treatment. Plasma-treated Kevlar fiber shooed the maximum critical surface tension and polar term, whereas the untreated PBO fiber showed the minimum value. Microfibril fracture pattern of plasma-treated Kevlar fiber appeared obviously. Based on the propagation of microfibril failure toward core region. the number of AE events for plasma-treated PBO and Kevlar fibers increased significantly. The results oi nondestructive AE were consistent well with microfailure modes by optical observation in microdroplet and two-fiber composites tests.

Reduction of Microbial Load on Radish (Raphanus sativus L.) Seeds by Aqueous Chlorine Dioxide and Hot Water Treatments (이산화염소수 및 열수처리에 따른 무(Raphanus sativus L.) 새싹 종자의 미생물 제어 효과)

  • Park, Kee-Jai;Lim, Jeong-Ho;Kim, Ji-Hye;Jeong, Jin-Woong;Jo, Jin-Ho;Jeong, Seong-Woong
    • Food Science and Preservation
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    • v.14 no.5
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    • pp.487-491
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    • 2007
  • This study was conducted to investigate the effect of treatment with squeous chlorine dioxide and hot water on the germination of radish (Raphanus sativus L.) seeds, and reduction of microbial load on the seeds. Increases in treatment and the concentration of aqueous chlorine dioxide in water resulted in increasing reductions in the counts of total aerobic microbes. Seeds treated with aqueous chlorine dioxide (100 ppm/20min, 200ppm/20min) showed about a 10-fold decrease in microbial loads. Germination of seeds was not adversely affected by any treatment tested, although the germination rate of seeds in the group treated at $55^{\circ}C$ for 20 min was reduced by 10% compared to that of control. Combined treatment with hot water and aqueous chlorine dioxide yielded better out comes in both microbial reduction and seed germination rate than did single treatments. A combined treatment with 100 ppm aqueous chlorine dioxide and hot water($45^{\circ}C$ or $50^{\circ}C$) resulted in about a 100-fold decrease in microbial load whereas germination rate showed only a slight increase to $97.0{\sim}97.7%$. Total aerobic microbial counts in radish seeds were decreased by aqueous chlorine dioxide and hot water treatment in the order. aqueous $CIO_2$+ hot water > aqueous $CIO_2$ > chlorinated water > hot water > control.

A Study on Mixed Noise Removal using Standard Deviation and Noise Density (표준편차 및 잡음 밀도를 이용한 복합잡음 제거 알고리즘에 관한 연구)

  • Kwon, Se-Ik;Kim, Nam-Ho
    • Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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    • 2017.10a
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    • pp.173-175
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    • 2017
  • With the rapid progress of the digital area has come the increase in demand for multi-media services. Imaging processing as a result is being hailed as a technological field that can offer smart and efficient methods for the processing and analysis of images. In general, noise exist in various types, depending on the cause and form. Some leading examples of noise are AWGN(additive white Gaussian noise), salt and pepper noise and complex noise. This study suggests an algorithm to remove complex noise by using the standard deviation and noise density of the partial mask in order to effectively remove complex noise in images.

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Overview of Automatic Spacing and Compound Noun Decomposition: 2018 Korean Natural Language Processing Contest (자동띄어쓰기 오류 수정 및 복합명사 분해 개요: 2018 차세정 언어처리 경진대회)

  • Choi, Jin-Hyuk;Ryu, Pum-Mo;Oh, Hyo-Jung
    • Annual Conference on Human and Language Technology
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    • 2018.10a
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    • pp.193-196
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    • 2018
  • 차세대정보컴퓨팅 개발사업 협의회에서 주최하고 한국어 정보처리 원천기술 연구개발 사업단에서 주관하는 2018 차세정 언어처리 경진대회가 개최되었다. "한국어 자동 띄어쓰기"와 "한국어 복합명사 분해"의 두 태스크로 진행되었고 각각 4팀, 2팀이 참가하였다. 주최 측에서 제공한 데이터만을 활용하는 closed 트랙과 각 참가팀이 추가 데이터를 활용하는 open 트랙으로 구분하여 평가하였다.

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