• Journal of Aquaculture
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• v.13 no.2
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• pp.129-135
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• 2000

Genetic variabilities of local populations of Anthocidaris crassispina and Hemicentrotus pulcherrimus were analysed by random amplified polymorphic DNA (RAPD) technique with phenotypic variabilities in timing of gonadal maturation, reproductive output and size of individuals in Cheju. H, pulcherrimus, collected from 4 locations during March 1997, indicated that Daepo individuals were significantly smaller than that at Hamdok, Wimi site A and Wimi site B (ANOVA, p>0.001). Gonodal-somatic index (GSI) of the Wimi site B population was significantly higher than that of three other locations (ANOVA, P>0.0001). An ANOVA test conducted on test size of A. crassispina, harvested from six different locations of Cheju during June 1997, indicated that the size of individuals from Pophwan was significantly smaller (P>0.0001) than that from five other locations. GSIs of urchin m Wimi and Hanlim were significantly higher than that from Pohwan and Oedo (ANOVA, p>0.0001). Genetic similarity, calculated from k13 primer analysis of total DNA, among the six different populations of A. crassispina varied from 0.67 to 0.92, and the values for H. pulcherrimus from 0.60 to 0.73; thus there was no genetic variation among different populations of the same species. Therefore, the populations are genetically homologous and the observed phenotypic variabilities were possibly associated with water temperature and food.

• Journal of the Korean Society of Food Science and Nutrition
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• v.41 no.4
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• pp.550-560
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• 2012

This study was performed to investigate the physicochemical properties of sea urchin ($Anthocidaris$ $crassispina$, $Pseudocentrotus$ $depressus$, $Hernicentrotus$ $pulcherrimus$) roe as a processed or canned food by steaming treatment. Proximate compositions of $A.$ $crassispina$ roe and $P.$ $depressus$ roe were similar, but water, crude ash, and carbohydrate contents of $H.$ $pulcherrimus$ roe showed little differences. Proximate compositions of sea urchin roe showed slight differences with steaming time, raw samples showed no differences. Glycine content of the three raw sea urchin roe samples showed the highest concentration among free amino acids, followed by arginine, alanine, and lysine, in order. Total free amino acid contents of raw sea urchin roe were 754.70 mg% ($A.$ $crassispina$), 567.75 mg% ($P.$ $depressus$), and 449.44 mg% ($H.$ $pulcherrimus$). Total free amino acid content of 5 min steaming sample was highest among steaming and canning conditions. ATP, ADP, and AMP contents of raw $P.$ $depressus$ roe sample was higher than those of $A.$ $crassispina$and $H.$ $pulcherrimus$ roe. Major fatty acids of the three raw sea urchin roe samples were myristic acid, palmitic acid, and EPA. S.F.A. content of raw samples of $A.$ $crassispina$and $H.$ $pulcherrimus$ roe was higher than U.F.A content, whereas U.F.A. content of $P.$ $depressus$ roe was highest among the three raw samples. For minerals K, P, Fe, and Zn contents were highest in $A.$ $crassispina$roe while Ca, Mg, Na, and Cu contents were highest in $H.$ $pulcherrimus$ roe. For heavy metals, Cd, Pb and As were detected in all samples in trace amounts under the criteria of the Korea food codex.

• Journal of Aquaculture
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• v.21 no.3
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• pp.139-145
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• 2008

Morphological classification of echinoid species has many difficulties because of their phenotypic variations. In the present study, we analyzed morphotypes and partial mitochondrial 12S rDNA sequences of four sea urchin species classified as Pseudocentrotus depressus, Anthocidaris crassispina, Hemicentrotus pulcherrimus and Strongylocentrotus nudus, and unidentified four species collected from the coasts of the East sea. Their genomic DNAs were extracted from gonads and mitochondrial 12S rDNA sequences were amplified by the polymerase chain reaction (PCR) method. The sequence identities among the known four sea urchin species were 87.4-95.6%. The sequence identities among the unidentified four species were 99.4-99.6% and showed the highest homology to S. intermedius(99.8%). Thus, our phylogenetic tree indicates that the unidentified four species belong to S. intermedius.

• Proceedings of the Korean Society of Fisheries Technology Conference
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• 1997.06a
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• pp.209.1-210
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• 1997

• Korean Journal of Fisheries and Aquatic Sciences
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• v.15 no.4
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• pp.345-358
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• 1982

1980.8-1982.8월까지 일광, 주문진, 돌산, 어청도 및 성산포에서 채집한 1,663미의 보라성게의 성장 및 산란에 관한 조사이다. 1세부터 5세까지의 평균각경은 가각 19, 32, 43, 51, 58mm로 추정됐으며 $l_\infty=84.4 mm,\;k=0.223,\;t_o=-0.187$의 성장식을 보였다. 가경에 대한 각고, 보대, 간보대, 위홍문부경 및 극 길이의 상관관계는 매우 높았다. 연령에 따른 가부분의 상대성장은차이가 없으나 극의 성장에서는 3세까지는 각경에 비례하여 성장하나 그 이후의 연령부터는 반비례하고 있다. 일광해역에서의 산란은 6-10월에 일어나며 주 산란은 8월로 사료한다. 연령에 따른 주 산란시기는 다르며 고연령군은 저연령군 보다 일찍 산란하고 있다. 제주산 보라성게는 8월 이전에 산란을 이미 마친 상태이며 여수산, 일광산은 주로 8월에, 주문진산, 어청도산은 8월 이후에 산란하는 것으로 판단된다.

• Proceedings of KOSOMES biannual meeting
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• 2017.11a
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• pp.243-243
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• 2017

• Proceedings of the Korean Environmental Sciences Society Conference
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• 2003.11b
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• pp.202-205
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• 2003

• Proceedings of the Korean Society of Fisheries Technology Conference
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• 1997.06a
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• pp.139-140
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• 1997

• Journal of Aquaculture
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• v.11 no.3
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• pp.401-419
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• 1998

The structure of benthic algal flora and feeding behavior of sea urchins (Anthocidaris crassispina and Hemicentrotus pulcherrimus), were investigated from the eight different locations along the coast of Cheju Island, Korea. Biomass distribution and dominance of the algal communities were studied in relation to the gut contents and population parameters of the sea urchins. As a result, although the largest algal biomass and biological characteristics of sea urchins, such as test diameter, test hight, body weight, gonad weight, etc. This inhdicated that sea urchins would not be dependednt sorely on the abundance of algal foods, and they might feed on other food items. In order to know such a trend with regard to food selectivity, the diets consumed by sea urchins were analyzed, and it was found that they were composed of benthic and drifted algae, various animal components (sponges, bryozoans, crustaceans, and various unidentified capsulated eggs, etc.) and biets turned out to be smaller than might be expected, and it is postulated that sea urchins were not likely to be the major grazing animals for the formation of algal bed and also canopy deterioration, so-called "white incrustation."

• Korean Journal of Food Science and Technology
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• v.47 no.4
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• pp.460-467
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• 2015

Sea urchin roe obtained from Anthocidaris crassispina and Pseudocentrotus depressus was briefly salt-fermented (5%), followed by ethanol treatment (1%) and the physicochemical properties as well as antioxidant activity were investigated. Compared to raw sea urchin roes, the salted one showed a significantly low amount of water (p<0.001) high salinity (p<0.05), ash content (p<0.001) and Na content (p<0.001). With salt-fermentation, the redness (p<0.05) and yellowness (p<0.001) of roe decreased noticeably, indicating the decomposition of endogenous carotenoids. Accordingly, the salted roe showed a lower DPPH radical scavenging activity than its unsalted counterpart. Additionally, it showed a significantly lower metal-chelating activity (p<0.05) and metal chelator content (e.g. ortho-phenolics) displayed by a negligible difference in titratable acidity. The salted roe showed significantly increased hardness (p<0.05) and total reducing capacity (p<0.001), which were attributed to the protein coagulation and the release of antioxidants bound to macromolecules after the ethanol treatment, respectively.