• Journal of Food Hygiene and Safety
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• v.31 no.6
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• pp.471-475
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• 2016

Salmonella Typhimurium (S. Typhimurium) is one of the common food pathogens which may cause gastroenteritidis in human and animals. The microorganism also causes the economic loss in animal farming and food industry. In this study, the disinfection efficacy of a powder disinfectant containing phosphate compounds as a main ingredient, was evaluated against S. Typhimurium. A bactericidal efficacy test by broth dilution method was used to determine the lowest effective dilution of the disinfectant following exposure to test bacteria for 30 min at $4^{\circ}C$. The disinfectant and test bacteria were diluted with hard water (HW) or organic matter suspension (OM) according to treatment condition. On HW and OM conditions, the bactericidal activity of the disinfectant against S. Typhimurium was 4- and 2-fold dilutions, respectively. As the disinfectant composed to phosphate compounds possesses bactericidal efficacy against pathogenic bacteria such as S. Typhimurium, the powder disinfectant can be used to control the spread of bacterial diseases.

• Journal of Korean Academy of Nursing
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• v.24 no.2
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• pp.226-240
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• 1994

본 연구의 목적은 한국의 사회심리적 간호현상을 밝힘에 있다. 이를 위한 자료는9명의 임상경험이 많은 병원 간호사들을 심층면담하여 수집하였으며, 분석은 해석학적 현상학과 근거 이론 방법에서 사용하는 계속비교 분석 방법을 이용하였다. 사회심리적 간호는 ‘정보제공’, ‘위로’, ‘상담’, ‘지도’의 네가지 범주로 구분되었다. 이 중에서 정보제공이 가장 많이 사용되었고 중요하게 여겨 진 반면, 상담과 지도는 흔히 사용되지 않았다. 이는 상담과 지도는 고도의 의사소통 기술, 인간관계에 대한 이해, 그리고 타인에 대한 감성등이 요구되었기 때문이다. 사회심리적 간호제공에 방해를 주는 요인도 밝혀졌다. 첫째, 가족이나 보호자들의 상주로 인해 간호사들이 사회심리적 간호의 임을 이들에게 떠넘기는 경향이 있었다. 둘째, 간호사의 특성, 즉 치료적 인간관계를 확립할 수 있는 간호사의 능력부족이 방해요인이었다. 셋째, 신체적 간호만을 중시하고 높은 간호사대 환자 비율을 가진 병원 시스템이 방해요인이었다. 넷째, 조밀한 병상등의 병원환경도 해요인으로 나타났다. 사회심리적 간호는 간호사-환자-보호자 관계를 치료적으로 형성 유지할 수 있는 간호사의 능력에 따라 결정되었기 때문에 이에 대한 분석을 계속하였으며, 치료적 관계형성과 유지에 영향을 주는 요인은 다음과 같이 나타났다. 첫째, 간호사의 기술적, 신체적 간호의 유능성이 치료적 관계형성에 필요하였다. 둘째, 환자 및 보호자와 신뢰관계를 구축할 수 있는 능력이 필요하였다. 셋째, 환자의 요구에 따르는 역할을 제대로 수행할 수는 능력이 필요하였다. 즉, 치료적 관계형성은 환자와의 신뢰형성만으로는 부족하며, 환자와 보호자의 요구에 따르는 역할, 즉 정보제공자, 위로자, 상담자, 지도자의 역할까지도 수행할 수 있어야 함을 의미한다. 이 외에도, 간호사들이 치료적 관계를 형성하고 유지하기 위하여 사용한 대책들을 제시하고 논의하였다. 본 연구는 한국의 사회심리적 간호의 범주, 방해요인, 촉진요인 등을 설명하고 기술하였기에, 우리나라의 간호사들이 사회심리적 간호를 위해 어떠한 일들을 주로 수행하며, 어떻게 환자 및 보호자들과 상호작용하면서 간호중재를 펴나가는가를 이해하는데 도움을 주리라 여겨진다.

• The Korean Journal of Pesticide Science
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• v.18 no.3
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• pp.181-190
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• 2014

An entomopathogenic bacterium, Bacillus thuringiensis (Bt), can sporulate along with production of insecticidal Cry toxins. Bt Cry toxins exhibit relatively narrow spectrum to target insects due to their specific interactions with midgut receptors. This study designed several strategies to enhance Bt efficacy in target insect spectrum and insecticidal activity. Four Cry toxins were purified from four different Bt strains and showed relatively narrow target insect spectrum. However, the Cry mixtures significantly expanded their target insect spectra. The additional effect of baculovirus to Cry toxin was tested with recombinant baculoviruses expressing Cry1Ac or Cry1Ca. However, the baculovirus was little effective to expand target insect spectrum. Bacterial culture broth of Xenorhabdus nematophila (Xn) significantly suppressed insect cellular immune response and increased Cry toxicity. The addition of Xn culture broth to Cry mixture significantly enhanced Bt efficacy in target insect spectrum and insecticidal activity.

• Korean Journal of Organic Agriculture
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• v.26 no.1
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• pp.129-140
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• 2018

Pectobacterium carotovorum subsp. carotovorum was found to be highly virulent to various vegetables, including Chinese cabbage. The antibacterial isolate CAB12243-2 was tested in a field bioassay for suppressing soft rot disease. The nucleotide sequencing of the 16S rRNA gene identified, the CAB12243-2 strain used in this study as Bacillus toyonensis. B. toyonensis CAB12243-2 inhibited the pectate lyase process by soft rot pathogens, and used trehalose and glucose as carbon sources. In field tests, the antibacterial isolate B. toyonensis CAB12243-2 suppressed soft rot disease with 73.0% control efficacy on the spring cultivar "Norangbom" and with 68.9% efficacy on the fall cultivar "Bulam 3". These results suggest that B. toyonensis CAB12243-2 can be used as a biological control agent for the control of soft rot diseases on vegetables.

• Journal of Sericultural and Entomological Science
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• v.14 no.2
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• pp.81-92
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• 1972

Studies on Pathogenicity of Nosema bombycis Naegeli are summarized as follows: 1. The mortality of the parents, Jam 103 and Jam 104, is remarkbly higher than that of the hybrid, Jam 103$\times$Jam 104, whereas there is no difference in the mortality between the parents. 3. In the mortality of the pathogen-concentration, it is increased in order of the following concentrations inoculated, 10$^{8}$ , 10$^{7}$ , 10$^{8}$ and 10$^{5}$ /ml. 3. In the mortality of each instar, it is high in order of 5th, 4th, 3rd, and 2nd instar. 4. In the interaction between the mortalities of the varieties and the concentrations, 1) The mortality shows no differences between the parents and the hybrid in the high concentration of 10$^{8}$ /ml. 2) The mortality of the hybrid is lower than that of the parents in the low concentration of 10$^{5}$ /ml, whereas no difference is found between the parents. 3) The interaction appears at the same level in the middle concentration of 10$^{6}$ /ml to the parents and of 10$^{7}$ /ml to the hybrid. 5. It was pointed out that active immunity depends upon the volume of antigen injection, immunizing period, and injection intervals. In this experiment, it is noticed that the optimum volume of injection is above 20ml and D is the best one of the four treatment (A.B.C.D.). 6. The immune sera indicate such a 12,800 high titer in the indirect method can be obtained from the D immunizing method. Silkworm tissues and N. bombycis spores show self-fluorescence, but it is able to distinguish it from the F.I.T.C. by using the U.V. filter. 7. The midgut epithelium is examined to be the first site of the tissues which are penetrated into and multiplied by the inoculation of the pathogen per os.

• The Korean Journal of Mycology
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• v.41 no.2
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• pp.112-117
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• 2013

Phytophthora capsici is one of major limiting factors in production of pepper and other important crops worldwide by causing foliage blight and rot on fruit and root. Increased demand for the replacement of fungicides has led to searching a promising strategy to control the fungal diseases. To meet eco-friendly agriculture practice, we isolated microorganisms and assessed their beneficial effects on plant health and disease control efficacy. A total of 360 bacterial strains were isolated from rhizosphere soil of healthy pepper plants, and categorized to 5 representative isolates based on colony morphology. Among the 5 bacterial strains (GJ-1, GJ-4, GJ-5, GJ-11, GJ-12), three bacterial strains (GJ-1, GJ-11, GJ-12) presented antifungal activity against P. capsici in an fungal inhibition assay. In phosphate solubilization and siderophore production, the strain GJ-1 was more effective than others. The strain GJ-1 was identified as Bacillus sp. using 16S rDNA analysis. Bacillus sp. GJ-1 was also found to be effective in inhibiting other plant pathogenic fungi, including Rhizoctonia solani, Pythium ultimum and Fusarium solani. Therefore, the Bacillus sp. GJ-1 can serve as a biological control agent against fungal plant pathogens.

• Journal of Life Science
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• v.31 no.7
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• pp.641-653
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• 2021

The purpose of this study was to confirm the antifungal activity, plant growth-promoting activity, and mineral solubilizing activity of 18 types of bacteria isolated purely from rhizosphere soil. The potential of isolates of the genus Bacillus and Pseudomonas as biocontrol agents was confirmed through the antifungal activity of these isolates. This activity has been determined to be due to various hydrolytic enzymes on the cell wall of plant pathogenic fungi and the production of siderophores in isolates. In addition, most of the isolates have been found to have aminocyclopropane-1-carboxylate deaminase production activity, indole-3-acetic acid production activity, and nitrogen fixation activity. These characteristics are believed to have a positive effect on root development, growth, and the productivity of crops via a reduction in the concentration of ethylene under conditions of environmental stress, to which plants are commonly exposed. In addition, on testing for the solubilizing activity of the isolates for phosphoric acid, silicon, calcium carbonate, and zinc, some isolates were found to have mineral solubilizing activities. Inoculation of these isolates during plant growth is expected to assist plant growth by converting nutrients necessary for growth into usable forms that can be absorbed by plants. The 18 isolated strains can be used as biocontrol agents due to their antifungal activity, plant growthpromoting activity, and mineral solubilizing activity.

• Sleep Medicine and Psychophysiology
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• v.25 no.2
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• pp.51-57
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• 2018

Objectives: Previous studies have shown that periodic limb movements in sleep (PLMS) could be one of risk factors for cardiovascular morbidity. The purpose of this study was to investigate the association between PLMS and blood pressure changes during sleep. Methods: We analyzed data from 358 adults (176 men and 182 women) aged 18 years and older who were free from sleep apnea syndrome (Respiratory Disturbance Index < 5) and sleep disorders such as REM sleep behavior disorder or narcolepsy. Demographic characteristics, polysomnography records, and clinical variable data including blood pressure, body mass index, alcohol, smoking, and current medications were collected. In addition, self-report questionnaires including the Beck Depression Index, Epworth Sleepiness Scale and Pittsburgh Sleep Quality Index were completed. Blood pressure change from bedtime to awakening was compared between the two periodic limb movement index (PLMI) groups [low PLMI ($PLMI{\leq}15$) and high PLMI (PLMI > 15)]. Blood pressure change patterns were compared using repeated measures analysis of variance. Results: Systolic blood pressure in the high PLMI group was lower than that in the low PLMI group (p = 0.036). These results were also significant when adjusted for gender and age, but were not statistically significant when adjusted for BMI, alcohol, smoking, anti-hypertension medication use and sleep efficiency (p = 0.098). Systolic blood pressure dropped by 9.7 mm Hg in the low PLMI group, and systolic blood pressure in the high PLMI group dropped by 2.9 mm Hg. There was a significant difference in delta systolic blood pressure after sleep between the two groups in women when adjusted for age, BMI, alcohol, smoking, antihypertensive medication use and sleep efficiency (p = 0.023). Conclusion: PLMS was significantly associated with a decreasing pattern in nocturnal BP during sleep, and this association remained significant in women when adjusted for age, BMI, alcohol, smoking, antihypertension medication use and sleep efficiency related to blood pressure. We suggest that PLMS may be associated with cardiovascular morbidity.

• The Korean Journal of Pesticide Science
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• v.11 no.4
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• pp.305-312
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• 2007

It was the investigated the response to metalaxyl of Phytophthora capsici isolates collected in 2005 and 2006. With effective concentrations ($EC_{50}$) of metalaxyl causing 50% growth inhibition, resistance baseline was determined as more than $1.0\;{\mu}g\;mL^{-1}$. Based on the resistance baseline, isolation frequency (%) of P. capsici resistant to metalaxyl was 29.0% and 218% in 2005 and 2006, respectively. Among the isolates of P. capsici obtained in 2006, the isolation was variable; 33.3% in Chungnam, 26.3% in Chungbuk and 11.1% in Gyeongbuk. Two isolates of metalaxyl-sensitive (MS) and too isolates of metalaxyl-resistant (MR) P. capsici were selected and then used to investigate the activity of metalaxyl to their development stages. Even though there was a difference in mycelial growth inhibition by metalaxyl between MS and MR isolates, the fungicide was not active or nearly to sporangium germination, zoospore release, and zoospore germination of both MS and MR isolates. However, the fungicide showed weak activity against sporangium germination and zoospore release of P. capsici, not related with its resistance. Also, it was not inhibitory to zoospore germination of both resistant and sensitive isolates. In a greenhouse test, it showed 100% of control value against P. capsici 06-86 sensitive to metalaxyl, when it was applied by soil-drenching at $25\;{\mu}g\;mL^{-1}$. However, 06-130 and 16-155 resistant to metalaxyl showed less than 20% of control value.

• Applied Chemistry for Engineering
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• v.34 no.5
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• pp.479-487
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• 2023

Copper is cost-effective and abundantly available as a biocidal coating agent for a wide range of material surfaces. Natural oxidation does not compromise the efficacy of copper, allowing it to maintain antimicrobial activity under prolonged exposure conditions. Furthermore, copper compounds exhibit a broad spectrum of antimicrobial activity against pathogenic yeast, both enveloped and non-enveloped types of viruses, as well as gram-negative and gram-positive bacteria. Contact killing of copper-coated surfaces causes the denaturation of proteins and damage to the cell membrane, leading to the release of essential components such as nucleotides and cytoplasm. Additionally, redox-active copper generates reactive oxygen species (ROS), which cause permanent cell damage through enzyme deactivation and DNA destruction. Owing to its robust stability, copper has been utilized in diverse forms, such as nanoparticles, ions, composites, and alloys, resulting in the creation of various coating methods. This mini-review describes representative coating processes involving copper ions and copper oxides on various material surfaces, highlighting the antibacterial and antiviral properties associated with different oxidation states of copper.