• Proceeding of EDISON Challenge
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• 2017.03a
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• pp.708-711
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• 2017

전기적 신호의 이상으로 발생하는 심방 부정맥은 심방세동으로 발전하는 대표적 심장 질환이다. 이러한 원인에는 세포 내 이온 채널의 유전적 결함으로 인한 기전이 알려져 있다. 지속적인 연구로 밝혀진 대표적인 유전적 질환 중 하나로서 KCNH2 유전자 돌연변이가 있다. 본 연구에서는 KCNH2 유전자 돌연변이가 심방부정맥을 유발하는 연관성연구를 기반으로 심실에서의 심장 질환 발현 연관성을 확인하고 심실부정맥과 심실세동 가능성을 예측하였다. 이를 위해 Ten tusscher 세포 모델에 KCNH2 유전자의 N588K, L532P 변이를 적용하여 2차원과 3차원 시뮬레이션을 진행하였다. wild-type(WT)과 mutant-type(MT)의 전기전도 패턴을 비교했다. 그 결과 WT의 전도파형이 일찍 자가소멸(self-termination) 되는 것과 대조적으로 MT는 회귀성 파형이 유지되었다(WT : 3.6초간 유지, MT : 지속적). 따라서 본 연구를 통해 KCNH2 유전자 돌연변이가 심실 조직의 취약성 (Action Potential Duration 감소, WT : 270 ms, N588K : 130 ms, L532P : 100 ms)을 증가시켜 부정맥의 요인이 됨을 확인하였다.

• Korean Journal of Soil Science and Fertilizer
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• v.18 no.2
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• pp.128-133
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• 1985

Variability of soil characteristics and purity were statistically summarized within mapping units and estimates were given for sample sizes required to reach predetermined levels of accuracy in mountainous area soils. Total variances within mapped soil series (expressed as CV): 30-60% for physical properties and CEC relatively affected by management and 80-230% for chemical properties except for pH affected by management. Means of some chemical properties were estimated on the assumption of log-normal distribution. These soils were correctly classified with regard to order at 73.9, to great group 56.9, and to series 47.4%. Approximate sample sizes required to detect the difference of 10 and 20% of the true mean with 0.95 confidence level were presented.

• Journal of the Korean School Mathematics Society
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• v.21 no.2
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• pp.183-206
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• 2018

It arises that teachers' professional competence should be considered not only with a cognitive perspective but also with a situative perspective. In this study, we considered mathematics teacher noticing as situational professional competencies of a mathematics teacher, and explored how mathematics teachers noticing on children's development of reasoning about variability in a video club has changed with the situative perspective. Findings illustrate that the 'interpreting' component among the three components of noticing-attending, interpreting, and deciding how to respond-was critically decisive for the change of the participant teachers' noticing. We also discussed how the video club intervention(the framework of children's development of reasoning about variability) can support the development of teacher noticing as a professional competence. This study has implications on the design of a video club to improve mathematics teacher noticing.

• Applied Biological Chemistry
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• v.41 no.1
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• pp.18-22
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• 1998

A mutant strain which hyperproduced thermostable ${\alpha}-amylase$ was obtained by chemical mutagenesis of Bacillus licheniformis. The mutant strain, SK-5, produced the enzyme about 50 times higher than the original strain. The mutant was longer and slimmer in shape, slower in growth compared to the original strain. Nucleotide sequence analysis of the SK-5 ${\alpha}-amylase$ gene revealed no changes in the the structural gene. The changes found in the promoter region might be responsible for the hyperproduction of the enzyme by the mutant. No structural changes in the enzyme structure could be observed when the secreted enzymes at various culture times were analyzed by Western blot.

• Environmental Mutagens and Carcinogens
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• v.10 no.2
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• pp.107-112
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• 1990

돼지 췌장 유래 엘라스타제의 돌연변이원성을 알아보기 위하여 포유동물 세포를 이용한 돌연변이 시험과 마우스를 이용한 소핵시험을 실시하였다. 엘라스타아제에 의하여 CHO-K1-BH4 cell이 6-thioguanine에 대해 내성을 가지게 되는 변이의 빈도를 조사하였다. 시험결과 엘라스타아제의 농도 증가 (0-30mg/ml)에 따른 변이빈도의 증가가 관찰되지 않았으며, 변이빈도도 음성대조군 변이빈도 치의 2배 이상을 보이지 않았다. 마우스를 이용한 소핵시험에서는 엘라스타아제를 1250, 2500 그리고 5000mg/kg의 용량으로 마우스에 1회 경구 투여 하였으며 양성대조군과 음성대조군에는 각각 mitomycin C 2mg/kg, 10skim milk 20ml/kg 을 각각 복강내와 경구로 투여하였다. 시험결과 엘라스타제 투여군의 소핵 출현빈도는 음성대조군과 비교하여 증가하지 않았다. 위의 결과로 보아 엘라스탄제는 이 시험계들에서 돌연변이원성이 없는 것으로 사려된다.

• Proceedings of the Korean Society for Food Science of Animal Resources Conference
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• 2004.10a
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• pp.223-226
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• 2004

오골계의 초기성장 단계별 근육과 근섬유의 변화를 살펴보면 흉근 무게는 부화 직후에서 3주령까지 증가가 가장 뚜렷했으며 근섬유의 크기도 증가하였다. 이러한 결과를 바탕으로 가슴 근육과 근섬유 변화는 오골계의 초기성장단계에 있어서 체중변화와 연관성이 높음을 확인하였으며, 핵산 함량 및 단백질 변이 분석을 통해 근육내 이화학적 변이 및 성장잠재력의 변이를 심도있게 분석할 수 있었다. 또한 가자미근의 경우 0주령에서 3주령까지developmental muscle fiber 의 감소 및 소진과 adult muscle fiber의 형성 및 증가도 각 개체의 체중, 근육의 무게, 성장특성 및 성장가능성과 연관성이 있어, 이후 오골계의 근육성장특성 분석 시 조직학적 분석기법의 활용도 및 이화학적 변이 분석의 참고자료 및 활용도가 클 것으로 기대된다.

• Korean Journal of Ecology and Environment
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• v.52 no.3
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• pp.274-283
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• 2019

Beta diversity is an efficient means of assessing the spatial variation in community composition among sites. To present fish community variation and LCBD (Local Contribution to Beta Diversity) among sites in stream, 6 sampling sites were selected in Cheonggye stream. Fish communities, environmental and habitat variables were collected at sites from April 2014 to October 2015. We used the total variance of the fish community data table (site-by-species community table) based on different forms, presence-absence, abundance, and Hellinger transformation, to estimate and compare beta diversity and LCBD. Fish community data table transformed by Hellinger distance showed the higher values of beta diversity than presence-absence and abundance data table. A similar patterns of LCBD were observed with presence-absence and Hellinger transformed data table. Low value of beta diversity calculated by community data table with abundance was due to the non-normality of fish assemblage data. Additionally, correlation coefficients were calculated to evaluate the relationships among LCBD, community indices and physicochemical variables. LCBD showed negative correlation coefficients with Shannon diversity. Overall, application of beta diversity analysis is an efficient method of addressing spatial variation of fish communities and ecological uniqueness of the sites in stream.

• Korean Journal of Soil Science and Fertilizer
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• v.18 no.3
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• pp.254-259
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• 1985

Variability of soil characteristics and purity were statistically summarized within mapping units and estimates were given for sample sizes required to reach predetermined levels of accuracy in mountain-foot slope soils. Total variances within mapped soil series (expressed as CV): 12-44% for pH, CEC, and particle size distribution was relatively unaffected and 56-196% for OM, ext.-cations, and available $P_2O_5$, affected by management. Means of some chemical properties were estimated on the assumption of lognormal distribution. These soils were correctly classified with regard to order at 52.2%, to great group 53.7%, and to series 39.7%. respectively. Approximate sample sizes required to detect the difference of 10 and 20% of the true mean with 0.95 confidence level were presented.

• Journal of Plant Biotechnology
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• v.47 no.1
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• pp.46-52
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• 2020

This experiment study aimed to identify the continuous genetic variation caused by benzyladenine (BA) treatment in strawberry tissue culture. The 'Goha' cultivar was used and treated with different concentrations of BA (0.0, 0.5, 1.0, 2.0 mg·L^-1). Morphological and genetic variation tests were performed, and genetic continuity tests were performed for three years. The morphological variation induced by BA was distinctively high (10.5 ~ 20.0%) and the genetic variation was 7.0 ~ 15.0%, 1.8 ~ 10.0%, and 5.0% in the first, second, and third year of cultivation, respectively. The rate of genetic variation decreased with increasing cultivation years. In addition, genetic variation caused by BA 1.0 mg·L^-1 and BA 2.0 mg·L^-1 occurred in the first and second years of cultivation, whereas only BA 2.0 mg·L^-1 caused genetic variation in the third year of cultivation. Therefore, a concentration of less than 1.0 mg·L^-1 BA was used for the propagation of strawberry tissue culture plants, and it was necessary to identify their variation.

• Korean Journal of Soil Science and Fertilizer
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• v.17 no.2
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• pp.141-146
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• 1984

Variation of soil characteristics and purity were examined within mapping unit and estimates were given for the number of samples required to reach predetermined levels of accuracy for black soil, VDBr. soil, DBr. soil, and brown soil in Jeju. Variation of chemical properties except pH and CEC was higher than that of physical properties. Brown soil showed the lowest variation and hence required the smallest sample size. The soils examined were correctly classified with regard to brown soil at purity 74.1, to DBr. soil 60.8, to black soil 55.6, and to VDBr. soil 46.9%. Sample calculation was presented which showed approximate sample sizes required to detect the difference of 10 and 20% of the mean with 0.95 confidence level.